TFIIA2 (GTF2A2) (NM_004492) Human Untagged Clone

CAT#: SC117338

GTF2A2 (untagged)-Human general transcription factor IIA, 2, 12kDa (GTF2A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GTF2A2
• Synonyms: HsT18745; T18745; TF2A2; TFIIA; TFIIA-12; TFIIA-gamma; TFIIAS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117338 sequence for NM_004492 edited (data generated by NextGen Sequencing)
  ATGGCATATCAGTTATACAGAAATACTACTTTGGGAAACAGTCTTCAGGAGAGCCTAGAT
  GAGCTCATACAGTCTCAACAGATCACCCCCCAACTTGCCCTTCAAGTTCTACTTCAGTTT
  GATAAGGCTATAAATGCAGCACTGGCTCAGAGGGTCAGGAACAGAGTCAATTTCAGGGGC
  TCTCTAAATACGTACAGATTCTGCGATAATGTGTGGACTTTTGTACTGAATGATGTTGAA
  TTCAGAGAGGTGACAGAACTTATTAAAGTGGATAAAGTGAAAATTGTAGCCTGTGATGGT
  AAAAATACTGGCTCCAATACTACAGAATGA
  
  Clone variation with respect to NM_004492.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004492 unedited
  TTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTTCTGCGGTTTCTGCG
  GCGGCTAGAGAGGTGGTCGGAGAAGTAGGAACCTCCTGCCGGGCTCGTGGCGGCTTCTGT
  CCGCTCCGCGGCGGGAAGCGCCTTCCCCACAGGACATCAATGCAAGCTTGAATAAGAAAA
  ACAAATTCTTCCTCCTAAGCCATGGCATATCAGTTATACAGAAATACTACTTTGGGAAAC
  AGTCTTCAGGAGAGCCTAGATGAGCTCATACAGTCTCAACAGATCACCCCCCAACTTGCC
  CTTCAAGTTCTACTTCAGTTTGATAAGGCTATAAATGCAGCACTGGCTCAGAGGGTCAGG
  AACAGAGTCAATTTCAGGGGCTCTCTAAATACGTACAGATTCTGCGATAATGTGTGGACT
  TTTGTACTGAATGATGTTGAATTCAGAGAGGTGACAGAACTTATTAAAGTGGATAAAGTG
  AAAATTGTAGCCTGTGATGGTAAAAATACTGGCTCCAATACTACAGAATGAATAGAAAAA
  ATATGACTTTTTTACACCATCTTCTGTTATTCATTGCTTTTGAAGAGAAGCATAGAAGAG
  ACTTTTTATTTATTCTAGAATTGCAGAAATGACTACACTGTGCTATACCAGAGAATTCCA
  GTAGAAAGAAACTTGTAACTCTGTAGCCTCTTACATCACCTTTATTATACAGCATGAAAA
  AACCATAACTTCTTTTTAGGGACAAAAGNTGNTGCCCTTCTAAGAACCTTNCTTATAAAC
  TCATNTAAAACTCTGAGTATAACTGGTGAAGCTATNAGGTAGCATATGAAGAATAGATAT
  GCATTTTCCATTTCTTGCAGGACATGATGCCTTTCAAATGCTATTCATAAGNCATGTTTG
  GC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004492 unedited
  AGGTCCTATCTCTGNACCGCGGCCCGCTTTCTAGNANCGAGTTTTTTCTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACACCCGACAAAACATGACTTTATTGA
  AATAAGCTTTCTGAAAGGGCATCATGTTCCTTGCAAAGGAAAGGGGAAAAGGCATTATCT
  AATATCTTCATATTGCTACCTTAATAGCTTCACCAGTTATTACTCAAAGTTTTAAAAGGA
  GTTTATTAAAAAAGGTTTTTAGGAAGGCAACAACTTTTGTCCTTAAAAAAAAGTTATGGT
  TTTTCATGCTGTATAAAAAAGGGGATGTAAGAGGCTACAGAGTTACAAGTTTCTTTTTAC
  TGGAATTCTCTGGTATAGCACAGGGTACTCATTTCTGCAATTCTAAAATAAATAAAAAGT
  CTCTTCTATGCTTCTCTTCAAAAGCAATGAATAACAGAAGATGGGGTAAAAAAGGCATAT
  TTTTTCTATTCATTCCGAACAATCGGACCCAGTATTTTTACCATCACAGGCTACAATTTT
  CACTTTATCCACTTTAATAAGTTCTGCCCCCCTTCTGAATTCACCATTCTTCAGTACAAA
  AGTCCACACATTATTCGCAAAATCTGTACCTATTTAAAGAGCCCCTGAAATTGACTCTGT
  TCCTGACCCTCTGAGCCCAGGCTGCATTTATAGCCTTATCAAACTGAAGTAAAACTTTGA
  GGCCACGTCGGGGGGCGATCCGTTGACACTGTATGAACCTATTTAGGCTTCCCTGAAGAT
  TGTTTCCCAAAAAACATTTTCGTATAACTGATATCCCGGGTCTTAGGAGGAAAAATTTGC
  TTTCTTATTAACTTCGGCTTGAGTCCCGCGGGGAAGCCTCTTCCCCCGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004492
• Insert Size: 920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004492.1, NP_004483.1
• RefSeq Size: 736 bp
• RefSeq ORF: 330 bp
• Locus ID: 2958
• Cytogenetics: 15q22.2
• Domains: TFIIA_gamma
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM, Mar 2008]'