GALNT3 (NM_004482) Human Untagged Clone

CAT#: SC117385

GALNT3 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3) (GALNT3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALNT3
• Synonyms: GalNAc-T3; HFTC; HFTC1; HHS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117385 sequence for NM_004482 edited (data generated by NextGen Sequencing)
  ATGGCTCACCTAAAGCGACTAGTAAAATTACACATTAAAAGACATTACCATAAAAAGTTC
  TGGAAGCTTGGTGCAGTAATTTTTTTCTTTATAATAGTTTTGGTTTTAATGCAAAGAGAA
  GTAAGTGTTCAATATTCCAAAGAGGAATCAAGGATGGAAAGGAACATGAAAAACAAAAAC
  AAGATGTTGGATTTAATGCTAGAAGCTGTAAACAATATTAAGGATGCCATGCCAAAAATG
  CAAATAGGAGCACCTGTCAGGCAAAACATTGATGCTGGTGAGAGACCTTGTTTGCAAGGA
  TATTATACAGCAGCAGAATTGAAGCCTGTCCTTGACCGTCCACCTCAGGATTCAAATGCA
  CCTGGTGCTTCTGGTAAAGCATTCAAGACAACCAATTTAAGTGTTGAAGAGCAAAAGGAA
  AAGGAACGTGGGGAAGCTAAACACTGCTTTAATGCTTTCGCAAGTGACAGGATTTCTTTG
  CACCGAGATCTTGGACCAGACACTCGACCTCCTGAATGTATTGAACAAAAATTTAAGCGC
  TGCCCTCCCCTGCCCACCACCAGTGTCATAATAGTTTTTCATAATGAAGCGTGGTCCACG
  TTGCTTAGAACTGTCCACAGTGTGCTCTATTCTTCACCTGCAATACTGCTGAAGGAAATC
  ATTTTGGTGGATGATGCTAGTGTAGATGAGTACTTACATGATAAACTAGATGAATATGTA
  AAACAATTTTCTATAGTAAAAATAGTCAGACAAAGAGAAAGAAAAGGTCTGATCACTGCT
  CGGTTGCTAGGAGCAACAGTCGCAACAGCTGAAACGCTCACATTTTTAGATGCTCACTGT
  GAGTGTTTCTATGGTTGGCTAGAACCTCTGTTGGCCAGAATAGCTGAGAACTACACGGCT
  GTCGTAAGTCCAGATATTGCATCCATAGATCTGAACACGTTTGAATTCAACAAACCTTCT
  CCTTATGGAAGTAACCATAACCGTGGAAATTTTGACTGGAGTCTTTCATTTGGCTGGGAG
  TCGCTTCCTGATCATGAGAAGCAAAGAAGGAAAGATGAAACCTACCCAATTAAAACACCC
  ACTTTTGCAGGAGGACTTTTTTCCATATCAAAAGAATATTTTGAGTATATTGGAAGCTAT
  GATGAAGAAATGGAAATCTGGGGAGGTGAAAATATAGAAATGTCTTTCAGAGTATGGCAA
  TGTGGTGGGCAGTTGGAGATTATGCCTTGCTCTGTTGTTGGACATGTTTTTCGCAGCAAA
  AGCCCTCATAGCTTTCCAAAAGGCACTCAGGTGATTGCTAGAAACCAAGTTCGCCTTGCA
  GAAGTCTGGATGGATGAATACAAGGAAATATTTTATAGGAGAAATACAGATGCAGCAAAA
  ATTGTTAAACAAAAAGCATTTGGTGATCTTTCAAAAAGATTTGAAATAAAACACCGCCTT
  CAGTGTAAAAATTTTACATGGTATCTGAACAACATTTATCCAGAGGTGTATGTGCCAGAC
  CTTAATCCTGTTATATCTGGATACATTAAAAGCGTTGGTCAGCCTCTATGTCTGGATGTT
  GGAGAAAACAATCAAGGAGGCAAACCATTAATTATGTATACATGTCATGGACTTGGGGGA
  AACCAGTACTTTGAATACTCTGCTCAACATGAAATTCGGCACAACATCCAGAAGGAATTA
  TGTCTTCATGCTGCTCAAGGTCTCGTTCAGCTGAAGGCATGTACCTACAAAGGTCACAAG
  ACAGTTGTCACTGGAGAGCAGATATGGGAGATCCAGAAGGATCAACTTCTATACAATCCA
  TTCTTAAAAATGTGCCTTTCAGCAAATGGAGAGCATCCAAGTTTAGTGTCATGCAACCCA
  TCAGATCCACTCCAAAAATGGATACTTAGCCAAAATGATTAA
  
  Clone variation with respect to NM_004482.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004482 unedited
  GGTTTGCGNATTTGTATACGATCATATAGGGCGGCCGCGAATTCGCACCAGTCCAGGCAA
  CGCGGGAGGCCCAGCGGGAAGGCAGGAGGCGGCGGCGGAGGAGGAGCTCTACTGAGCCGC
  AACTGTGGCGACAGCAACCGGAGTCGCAGCCGCCGCCACCTGCACCTGGCGCCTAGCCCA
  CGTCCAGCGCCTGCCCGGCCGCCGCTTCCCGCCACCCTGCCCTGCCCACCCGCCAGGTAC
  TACCATTAAAGATACCTTCTTCTCAGCAAATCTATGATAAAAAATATAAGTAACAGAAGA
  AGAAATAACTGTTATTTGTCAAGTGACAAGCTTTTAATGTCAGAATGGCTCACCTAAAGC
  GACTAGTAAAATTACACATTAAAAGACATTACCATAAAAAGTTCTGGAAGCTTGGTGCAG
  CAATTTTTTTCTTTATAATAGTTTTGGTTTTAATGCAAAGAGAAGTAAGTGTTCAATATT
  CCAAAGAGGAATCAAGGATGGAAAGGAACATGAAAAACAAAAACAAGATGTTGGATTTAA
  TGCTAGAAGCTGTAAACAATATTAAGGATGCCATGCCAAAAATGCAAATAGGAGCACCTG
  TCAGGCAAAACATTGATGCTGGTGAGAGACCTTGTTTGCAAGGATATTATACAGCAGCAG
  AATTGAAGCCTGTCCTTGACCGTCCACCTCAGGATTCAAATGCACCTGGTGCTTCTGGTA
  AAGCATTCAAGACAACCAATTTAAGTGTTGAAGAGCANAAGGAAAAGGAACGTGNNGAAG
  CTAAACACTGCTNTAATGCTTTCGCAAGTGACAGGNATTCTTTGCACCGAGATCTGGNAC
  AGACACTCGACCTNCTGAATGTATNGNNAACAAAATTAAGCGCTGNCCTCCCCTGCCCCA
  CACAGTGTCATATTAGNNTTTCATAATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004482 unedited
  CTTGGCCGCGGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTAGGGT
  TGAGGATTTTTTATTGGGTTTTCCACATAGATAAAAAAAAAGGCTTTTTGATGAAAAGAA
  TCCATTACAAAGTCAAAAATCCATTACAATTATAATTGAATCAGTAACAAAATTTAGCTT
  TAAATGAGTCAAGTATTCTGCATTTGAAATTTAATATCACAAACATTCAAGATTAGTGAA
  TTTTGGTAACAAAAAAATACTAGAAGAAAGGAAAAGGACACCTTTTCAACAGATAGTAAT
  TTATAAAAATTTTTTTAAAAGTGCTTTGGGAAAACACACAGTATCATTACTTAAGAAAAG
  TCATTTAAGGAAGACTTAAGTGCTTCAAGTGGAGTGTATTACAGACTAAAAAATGTTTTA
  AAATTTGCCAAGAAATTTAAGTGTTAAAAATACTCTTCTCCTTATTCAGTTTCATGTTTA
  AGGAAACATTTGACAGACAAGTAAACCAAACGCAAAAAAAAGTTCACCTGCATTTTAAAC
  TAATAAATTCTGGATCTGTAAAAGCTCTTGGTTTGTACACAGAGGCCAATGCTGACATTT
  ATTGATCTATTTTTATGCAGCTAAAAAAATACAGTAACAAATCTTTCTTCCTATCCCCCC
  AAAAAACTAGGGGAAATATTTTAAATTGTGAGTGTGTGAATGTAGCTATATATATATATC
  CCCTAGTGTACAAAACACACAAACCTCACTTTACTTGGAAAATATTCTCATCATACTGAA
  AACATCTTTTCCCCTACCTCTGGACATTTTGAAATAGTCTTCGGGACTACTAGTACTGGG
  CTTTGAAACAGACCCTTGCAAAATTCTGTACCGGGCCCCTAAGAAATAATACGAAAAGGC
  CTTGGAAAAGCTCACTTACCCGCTCGCATATCTCCCAACCACCGGTGCCTACCCACTTAA
  AACACAAATTCCTTTTAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004482
• Insert Size: 4760 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004482.2, NP_004473.1
• RefSeq Size: 3874 bp
• RefSeq ORF: 1902 bp
• Locus ID: 2591
• Cytogenetics: 2q24.3
• Domains: RICIN, Glycos_transf_2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: 'This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities. [provided by RefSeq, Jul 2008]'