DDX6 (NM_004397) Human Untagged Clone

CAT#: SC117398

DDX6 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX6
• Synonyms: HLR2; P54; RCK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117398 sequence for NM_004397 edited (data generated by NextGen Sequencing)
  ATGAGCACGGCCAGAACAGAGAACCCTGTTATAATGGGTCTGTCCAGTCAAAATGGTCAG
  CTGAGAGGCCCTGTGAAACCCACTGGTGGCCCTGGAGGAGGGGGCACACAGACACAGCAA
  CAGATGAACCAGCTGAAAAACACCAACACAATCAATAATGGCACTCAGCAGCAAGCACAG
  AGTATGACCACCACTATTAAACCTGGTGATGACTGGAAAAAGACTTTAAAACTCCCTCCA
  AAGGATCTAAGAATCAAAACTTCGGATGTGACCTCCACAAAAGGAAATGAGTTTGAAGAT
  TACTGTTTGAAACGGGAGTTACTGATGGGAATTTTTGAAATGGGCTGGGAAAAGCCATCT
  CCTATTCAGGAGGAGAGCATTCCCATTGCTTTATCTGGTAGGGATATCTTAGCTAGAGCA
  AAAAATGGAACAGGCAAGAGCGGTGCCTACCTCATTCCCTTACTTGAACGGCTAGACCTG
  AAGAAGGACAATATACAAGCAATGGTGATTGTTCCCACTAGAGAACTTGCTCTACAGGTC
  AGTCAAATTTGCATCCAGGTCAGCAAACACATGGGAGGGGCCAAAGTGATGGCAACCACA
  GGAGGAACCAATTTACGAGATGACATAATGAGGCTTGATGATACAGTGCACGTGGTGATT
  GCTACCCCTGGGAGAATCCTGGATCTTATTAAGAAAGGAGTAGCAAAGGTTGATCATGTC
  CAGATGATAGTATTGGATGAGGCAGATAAGTTGCTGTCACAGGATTTTGTGCAGATAATG
  GAGGATATTATTCTCACGCTACCTAAAAACAGGCAGATTTTACTATATTCCGCTACTTTC
  CCTCTTAGTGTACAGAAGTTCATGAATTCCCATTTGCAGAAACCCTATGAGATTAACCTG
  ATGGAGGAACTAACTCTGAAGGGAGTAACCCAGTACTACGCATATGTAACTGAGCGCCAA
  AAAGTACACTGCCTCAACACACTTTTCTCCAGGCTTCAGATAAACCAGTCGATCATTTTC
  TGTAACTCCTCTCAGCGAGTTGAATTGCTAGCCAAGAAGATTTCTCAACTGGGTTATTCT
  TGCTTCTATATTCATGCTAAAATGAGGCAGGAACATCGAAATCGTGTATTTCATGATTTC
  CGAAATGGCTTATGCCGCAATCTTGTTTGCACTGATCTGTTTACCCGAGGTATTGATATA
  CAAGCTGTGAATGTGGTAATAAACTTTGATTTCCCAAAGCTGGCAGAGACCTATCTCCAT
  CGTATTGGAAGATCAGGTCGCTTTGGTCATCTTGGCTTAGCCATCAACTTGATCACATAT
  GATGATCGCTTCAACCTGAAAAGTATTGAGGAGCAGCTGGGAACAGAAATTAAACCTATT
  CCGAGCAACATTGATAAGAGCCTGTATGTGGCAGAATACCACAGCGAGCCTGTAGAAGAT
  GAGAAACCTTAA
  
  Clone variation with respect to NM_004397.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004397 unedited
  ATCAAGTTATTATAGGGCGGCCGCGAATTCGGCACGAGGGCGACTTCGGCGGCGCCACGA
  GAGCGGGCAGCGGAGGAGATTGACGTGAGTGAATTCAGATATAACTCAAGCTTGTTAGAG
  GGCTTTTTAAAAATAAAAAGTTGATTCCGTGCAAGAGAGCAAGTTACTGCTGCTTACCGT
  TCAGAGACTTACAGGTGCTTGCCTGCATTGCAATAAAGGACTCATTTATTGAGCAAGACT
  TATATTTATCTCTTCATTTTGGAGAGCCTAATAAACTGTTATTACAGTTTCTCTACTGAC
  TTTCAAAAGTTTTGAAGTTTGAAAGACCTTTGCAATTAAAACAGCATGAGCACGGCCAGA
  ACAGAGAACCCTGTTATAATGGGTCTGTCCAGTCAAAATGGTCAGCTGAGAGGCCCTGTG
  AAACCCACTGGTGGCCCTGGAGGAGGGGGCACACAGACACAGCAACAGATGAACCAGCTG
  AAAAACACCAACACAATCAATAATGGCACTCAGCAGCAAGCACAGAGTATGACCACCACT
  ATTTAAACCTGGTGATGACTGAAAAAAGACTTTAAAACTCCCTCAAAGGATCTAAGAATC
  AAACTTCCGATGTGACCCTCCACAAAGGAAATGAGTTTGAAGATTACTGTTTGAAACGGA
  AGTTACTGATGGGAATTTTTGAAATGGGCTGGGAAAAGCCCTCTCCTATTCAGCANGAAA
  GCATTCCCATTGTTTATCTGGTAGGGATATCTTAGCTAGAGCAAAAAAATGGACCAGGCA
  GAAGCGGGGCCTACCTTAATCCCTTAATTGAACGGGTTAGACCTGAAGAAGGACAATTTA
  CCAGCCAATGTTGATTGTGTCCCCTTAAAGACTTTGTTTTAAAGGTCAATCCAAAATTGC
  ATTCCCAGTCACCACACCCTTGTGGAAGGGGCCCAAGTGGAGGCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004397 unedited
  TTTGGACGCGGCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTTTTTTAAATA
  CATGTATTTACAGTGCGGATGAACTGCAGTTGCATATCAACTCCTCCCCAAAAAGAAAAA
  AAAAATGGTGTTTAACATTACTAGTGAGTTTCAACTTCTAGCCCTGGGCTCGAGTACTGG
  GTAGAAAGGGAAGAGACTTCAACCTGAAACCAAAATAAGAAGACATTTTTTACAAGAAAA
  AGAAAAAAAAACGGAAAGAAAGAGTTACTATTGTTGATTCCTCGGGCTGTGTCTAAAAGA
  TGATATTCTGAATGGTCTCATACCATAAGGCACTTCGCACAAATAAGTAATAAGCTCTTC
  AGGCTTAAAAAACGGATGAGGAATCAGGGAGAAGTTGAAATGCACGAGAGTCAGCTGTTG
  GAGAATTTTGAAATCATTGACAAGCTTGTGTGTTCATTAAGATTCTTCTCTTTTTAGGGT
  TTCTCCCCTTCTCTTTTTTCTTTTCCTTCCTTGTCCCCTTTCCCCAGAAACAATTTCTTA
  AAAACCAGCAGTTAGTGCAACTAATGTTCAGTCAGCACACAGTGCAAACAAGTGGAACAA
  AAAAGGTATATTCCTTCTTTTCACCCTTTCCCTCTTACCAGTTAAAAAAAGAAAATGCCC
  GAGCTCTTCTTTAAGTCTTCATAGCTCCAACATACAAGATGAACAACCCCCACAGAGACG
  ACCTTTCCCCCAAATCGATGCGCCACAGATCCAAACGAGCGCTTTGTCATTCGCCAAACC
  CTGCCTTGTCAAGGTTCTCTCATTCTTTACAGCGCCCGTTGGGCATTCCCGCCACATACC
  GGTTCCTCATAATGCGGCCCGAAAAAGCGCTAATTTTTGTTCCAGCTGGCCCTCCATACT
  TTTCAGCGTGGAGCGGATCCTATTCGCGAACACAGTGTAGGGCTAATCCCGCAGACCCAA
  GCGCCCTGATCTCCACTCCCACGAAATACGCCTCCCACCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004397
• Insert Size: 2540 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004397.3, NP_004388.1
• RefSeq Size: 4180 bp
• RefSeq ORF: 1419 bp
• Locus ID: 1656
• Cytogenetics: 11q23.3
• Domains: DEAD, helicase_C
• Protein Pathways: RNA degradation
• Gene Summary: 'This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.