BCS1L (NM_004328) Human Untagged Clone

CAT#: SC117432

BCS1L (untagged)-Human BCS1-like (S. cerevisiae) (BCS1L), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BCS1L
• Synonyms: BCS; BCS1; BJS; FLNMS; GRACILE; h-BCS; h-BCS1; Hs.6719; MC3DN1; PTD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117432 sequence for NM_004328 edited (data generated by NextGen Sequencing)
  ATGCCACTTTCAGACTTTATTCTGGCTCTGAAGGACAATCCCTACTTTGGGGCTGGATTT
  GGGCTGGTGGGTGTGGGCACAGCCCTGGCCCTGGCCCGGAAGGGTGTCCAACTGGGCCTG
  GTGGCATTCCGGCGCCATTACATGATCACACTGGAAGTCCCTGCTCGAGACAGGAGCTAT
  GCCTGGTTGCTTAGCTGGCTCACCCGCCACAGTACCCGTACTCAGCACCTCAGTGTCGAG
  ACTTCGTACCTTCAGCATGAGAGTGGCCGCATTTCCACTAAGTTTGAATTTGTCCCCAGC
  CCTGGAAACCATTTTATCTGGTATCGGGGGAAATGGATTCGGGTAGAACGAAGTCGAGAG
  ATGCAGATGATAGACTTGCAGACGGGGACTCCTTGGGAATCTGTCACCTTCACGGCCCTG
  GGCACTGACCGAAAGGTTTTCTTCAACATCCTGGAGGAAGCTCGAGAGCTAGCCTTGCAG
  CAGGAGGAAGGGAAGACCGTGATGTACACAGCTGTGGGCTCTGAATGGCGTCCCTTTGGC
  TATCCACGCCGCCGGCGACCACTGAATTCTGTGGTTCTACAACAGGGTCTGGCTGACCGA
  ATTGTCAGAGACGTCCAGGAATTCATCGATAACCCCAAGTGGTACACTGACAGAGGCATT
  CCTTACAGACGTGGCTACCTGCTTTATGGGCCCCCTGGTTGCGGAAAGAGCAGTTTTATC
  ACAGCCCTGGCTGGGGAACTGGAGCACAGCATCTGCCTGCTGAGCCTCACGGACTCCAGC
  CTCTCTGATGACCGACTCAACCACCTGCTGAGCGTGGCCCCGCAGCAGAGCCTGGTACTC
  CTGGAGGATGTGGATGCTGCTTTTCTCAGTCGAGACTTGGCTGTGGAGAACCCAGTAAAG
  TACCAAGGCCTAGGTCGCCTCACCTTCAGTGGACTGCTCAATGCCTTGGATGGTGTGGCT
  TCCACCGAGGCCCGCATCGTGTTCATGACCACCAACCACGTTGACAGGCTGGACCCTGCC
  CTGATACGCCCGGGGCGAGTGGACCTGAAGGAGTACGTGGGCTACTGCTCACACTGGCAG
  CTGACCCAGATGTTCCAGAGGTTCTATCCAGGGCAGGCACCTTCCTTAGCTGAGAACTTT
  GCAGAACATGTCCTTCGAGCTACAAACCAGATCAGTCCTGCCCAGGTGCAGGGCTACTTC
  ATGCTGTATAAAAATGACCCTGTAGGGGCAATTCACAATGCTGAGTCTCTGAGGAGGTGA
  
  Clone variation with respect to NM_004328.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004328 unedited
  NGGCAGTCAGAATTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACCAAATCGAGA
  CGGAGGGCCAGAGAGTCACGGCGGTTTTCGTAACACCCCAGGCCTGTAAGGTTTGGTGTT
  TCCCTTTCAAGATGCCACTTTCAGACTTTATTCTGGCTCTGAAGGACAATCCCTACTTTG
  GGGCTGGATTTGGGCTGGTGGGTGTGGGCACAGCCCTGGCCCTGGCCCGGAAGGGTGTCC
  AACTGGGCCTGGTGGCATTCCGGCGCCATTACATGATCACACTGGAAGTCCCTGCTCGAG
  ACAGGAGCTATGCCTGGTTGCTTAGCTGGCTCACCCGCCACAGTACCCGTACTCAGCACC
  TCAGTGTCGAGACTTCGTACCTTCAGCATGAGAGTGGCCGCATTTCCACTAAGTTTGAAT
  TTGTCCCCAGCCCTGGAAACCATTTTATCTGGTATCGGGGGAAATGGATTCGGGTAGAAC
  GAAGTCGAGAGATGCAGATGATAGACTTGCAGACGGGGACTCCTTGGGAATCTGTCACCT
  TCACGGCCCTGGGCACTGACCGAAAGGTTTTCTTCAACATCCTGGAGGAAGCTCGAGAGC
  TAGCCTTGCAGCAGGAGGAAGGGAAGACCGTGATGTACACAGCTGTGGGCTCTGAATGGC
  GTCCCTTTGGCTATCCACGCCGCCGGCGACCACTGAATTCTGTGGTTCTACAACAGGGTC
  TGGCTGACCGAATTGTCAGAGACGTCCAGGAAATTCATCGATACCCCAAGTGGTACACTG
  ACANGAGCATTCCTTACAGACGTGGCTACCTGCTTTATGGCCCCNCTNNGGTGCGAAAGA
  CAGTTTTATCACAGCCCTGGNCTGGGAACTGGAGCACAGCATCTGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004328 unedited
  AAGTTAAAACCCTTTTTTTTTTTTTTTTTTTTTTTTTTAGTGGGGCAAATGTTTATTGAG
  CTAAAGGAAGAAAGCTGAACTGAGCCCAGCCTGATCACCTCCTCAAAAACTCAACATTGT
  GAATTGCCCCTACAGGGTCATTTTTATACAGCATGAAGTAACCCTGCACCTGGGCAGGAC
  TGATCTGGTTTGTAACTCGAAAGACATGTTCTGCAAAGTTCTCAACTAAAGAAGGTGCCT
  GCCCTGGATAAAACCTCTGGAACATCTGGGTCAGCTGCCAGTGTGAGCAGTAGCCCACGT
  ACTCCTTCAGGTCCACTCGCCCCGGGCGTATCAAGGCAGGGTCCAGCCTGTCAACGTGGT
  TGGGGGTCATGAACACGATGCGGGCCTCGGTGGAAGCCACACCATCCAAGGCATTGAGCA
  GTCCACTGAAGGTGAGGCGACCTAAGCCTTGGTACTTTACTGGGTTCTCCACAGCCAAGT
  CTCGACTGAAAAAAGCAGCATCCACATCCTCCAAGAGTACCAGGCTCTGCTGCGGGGCCA
  CGCTCAACAAGTGGTTGAGTCGGTCATCAAAGAGGCTGGAGTCCGTGAGGCTCAACAAGC
  AGATGCTGTGCTCCAGTTCCCCAGCCAGGGCTGTGATAAAACTGCTCTTTCCGCAACCAG
  GGGGCCCATAAAGCAAGTAGCCACGTCTGTAAGGAATGCCTCTGTCAGTGTACCACTTGG
  GGTTATCGATGAATTCCTGGACGTCTCTGACAATTCGGTCAGCCAGACCCTGGTGTAGGA
  CCACAAAATTCAGTGGTCCCCGGCCGCGTGGATAGCCAAAGGGACCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004328
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004328.3, NP_004319.1
• RefSeq Size: 1603 bp
• RefSeq ORF: 1260 bp
• Locus ID: 617
• Cytogenetics: 2q35
• Domains: AAA
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a homolog of the S. cerevisiae bcs1 protein which is involved in the assembly of complex III of the mitochondrial respiratory chain. The encoded protein does not contain a mitochondrial targeting sequence but experimental studies confirm that it is imported into mitochondria. Mutations in this gene are associated with mitochondrial complex III deficiency and the GRACILE syndrome. Several alternatively spliced transcripts encoding two different isoforms have been described. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, 3, 4, 5, and 7 all encode the same isoform (a).