Semaphorin 3F (SEMA3F) (NM_004186) Human Untagged Clone

CAT#: SC117510

SEMA3F (untagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (SEMA3F)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEMA3F
• Synonyms: SEMA-IV; SEMA4; SEMAK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117510 sequence for NM_004186 edited (data generated by NextGen Sequencing)
  
  
  ATGCTTGTCGCCGGTCTTCTTCTCTGGGCTTCCCTACTGACTGGGGCCTGGCCATCCTTCCCCACCCAGG
  ACCACCTCCCGGCCACGCCCCGGGTCCGGCTCTCATTCAAAGAGCTGAAGGCCACAGGCACCGCCCACTT
  CTTCAACTTCCTGCTCAACACAACCGACTACCGAATCTTGCTCAAGGACGAGGACCACGACCGCATGTAC
  GTGGGCAGCAAGGACTACGTGCTGTCCCTGGACCTGCACGACATCAACCGCGAGCCCCTCATTATACATT
  GGGCAGCCTCCCCACAGCGCATCGAGGAATGCGTGCTCTCAGGCAAGGATGTCAACGGCGAGTGTGGGAA
  CTTCGTCAGGCTCATCCAGCCCTGGAACCGAACACACCTGTATGTGTGCGGGACAGGTGCCTACAACCCC
  ATGTGCACCTATGTGAACCGCGGACGCCGCGCCCAGGCCACACCATGGACCCAGACTCAGGCGGTCAGAG
  GCCGCGGCAGCAGAGCCACGGATGGTGCCCTCCGCCCGATGCCCACAGCCCCACGCCAGGATTACATCTT
  CTACCTGGAGCCTGAGCGACTCGAGTCAGGGAAGGGCAAGTGTCCGTACGATCCCAAGCTGGACACAGCA
  TCGGCCCTCATCAATGAGGAGCTCTATGCTGGTGTGTACATCGATTTTATGGGCACTGATGCAGCCATCT
  TCCGCACACTTGGAAAGCAGACAGCCATGCGCACGGATCAGTACAACTCCCGGTGGCTGAACGACCCGTC
  GTTCATCCATGCTGAGCTCATTCCTGACAGTGCGGAGCGCAATGATGATAAGCTTTACTTCTTCTTCCGT
  GAGCGGTCGGCAGAGGCGCCGCAGAGCCCCGCGGTGTACGCCCGCATCGGGCGCATTTGCCTGAACGATG
  ACGGTGGTCACTGTTGCCTGGTCAACAAGTGGAGCACATTCCTGAAGGCGCGGCTCGTCTGCTCTGTCCC
  GGGCGAGGATGGCATTGAGACTCACTTTGATGAGCTCCAGGACGTGTTTGTCCAGCAGACCCAGGACGTG
  AGGAACCCTGTCATTTACGCTGTCTTTACCTCCTCTGGCTCCGTGTTCCGAGGCTCTGCCGTGTGTGTCT
  ACTCCATGGCTGATATTCGCATGGTCTTCAACGGGCCCTTTGCCCACAAAGAGGGGCCCAACTACCAGTG
  GATGCCCTTCTCAGGGAAGATGCCCTACCCACGGCCGGGCACGTGCCCTGGTGGAACCTTCACGCCATCT
  ATGAAGTCCACCAAGGATTATCCTGATGAGGTGATCAACTTCATGCGCAGCCACCCACTCATGTACCAGG
  CCGTGTACCCTCTGCAGCGGCGGCCCCTGGTAGTCCGCACAGGTGCTCCCTACCGCCTTACCACTATTGC
  CGTGGACCAGGTGGATGCAGCCGACGGGCGCTATGAGGTGCTTTTCCTGGGCACAGACCGCGGGACAGTG
  CAGAAGGTCATTGTGCTGCCCAAGGATGACCAGGAGATGGAGGAGCTCATGCTGGAGGAGGTGGAGGTCT
  TCAAGGATCCAGCACCCGTCAAGACCATGACCATCTCTTCTAAGAGGCAACAACTCTACGTGGCGTCAGC
  CGTGGGTGTCACACACCTGAGCCTGCACCGCTGCCAGGCGTATGGGGCTGCCTGTGCTGACTGCTGCCTT
  GCCCGGGACCCTTACTGTGCCTGGGATGGCCAGGCCTGCTCCCGCTATACAGCATCCTCCAAGAGGCGGA
  GCCGCCGGCAGGACGTCCGGCACGGAAACCCCATCAGGCAGTGCCGTGGGTTCAACTCCAATGCCAACAA
  GAATGCCGTGGAGTCTGTGCAGTATGGCGTGGCCGGCAGCGCAGCCTTCCTTGAGTGCCAGCCCCGCTCG
  CCCCAAGCCACTGTTAAGTGGCTGTTCCAGCGAGATCCTGGTGACCGGCGCCGAGAGATTCGTGCAGAGG
  ACCGCTTCCTGCGCACAGAGCAGGGCTTGTTGCTCCGTGCACTGCAGCTCAGCGATCGTGGCCTCTACTC
  CTGCACAGCCACTGAGAACAACTTTAAGCACGTCGTCACACGAGTGCAGCTGCATGTACTGGGCCGGGAC
  GCCGTCCATGCTGCCCTCTTCCCACCACTGTCCATGAGCGCCCCGCCACCCCCAGGCGCAGGCCCCCCAA
  CGCCTCCTTACCAGGAGTTAGCCCAGCTGCTGGCCCAGCCAGAAGTGGGCCTCATCCACCAGTACTGCCA
  GGGTTACTGGCGCCATGTGCCCCCCAGCCCCAGGGAGGCTCCAGGGGCACCCCGGTCTCCTGAGCCCCAG
  GACCAGAAAAAGCCCCGGAACCGCCGGCACCACCCTCCGGACACATGA
  
  Clone variation with respect to NM_004186.4
  42:t=>c 279:t=>c 1507:a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004186 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGAGTGGAG
  CCTGCTTCCTGGGCCCTAGGCCCCTCCCACAATGCTTGTCGCCGGTCTTCTTCTCTGGGC
  TTCCCTACTGACTGGGGCCTGGCCATCCTTCCCCACCCAGGACCACCTCCCGGCCACGCC
  CCGGGTCCGGCTCTCATTCAAAGAGCTGAAGGCCACAGGCACCGCCCACTTCTTCAACTT
  CCTGCTCAACACAACCGACTACCGAATCTTGCTCAAGGACGAGGACCACGACCGCATGTA
  CGTGGGCAGCAAGGACTACGTGCTGTCCCTGGACCTGCACGACATCAACCGCGAGCCCCT
  CATTATACATTGGGCAGCCTCCCCACAGCGCATCGAGGAATGCGTGCTCTCAGGCAAGGA
  TGTCAACGGCGAGTGTGGGAACTTCGTCAGGCTCATCCAGCCCTGGAACCGAACACACCT
  GTATGTGTGCGGGACAGGTGCCTACAACCCCATGTGCACCTATGTGAACCGCGGACGCCG
  CGCCCAGGCCACACCATGGACCCAGACTCAGGCGGTCAGAGGCCGCGGCAGCAGAGCCAC
  GGATGGTGCCCTCCGCCCGATGCCCACAGCCCCACGCCAGGATTACATCTTCTACCTGGA
  GCCTGAGCGACTCGAGTCAGGGAAGGGCAAGTGTCCGTACGATCCCAAGCTGGACACAGC
  ATCGGCCCTCATCAATGAGGAGCTCTATGCTGGTGTGTACATCGATTTTATGGGCACTGA
  TGCAGCCATCTTCCGCACACTTGGGAAGCAGACAGCCATGCGCACGGATCAGTACAACTN
  CCCGGTGGGCTGACGACCCCGTCGTTCATCCATGCCTGAGCTCATTNCCTGACAGTGCGG
  GAGCGCAATGATGATAAGCTTTACTTCTCCTTCCGTGAGCGGTCCGCAAAGGCGCGCAAA
  GCCCCGCGNTGTCGCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004186 unedited
  TTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAAGTTTTGGAGGAT
  GCTGCCACTGAGGCAGGCTATATTCAACCCACCAATAAATACTGTCTTTGCAGGGGCAGG
  GTGTGTATAGAATATAGATATTTTATATATATATATATATATATATATATATATCTTTTA
  TATTAAAAGGGACAAGGGCTAGGCTGGCCCATGGCAGGCACAGGCAGCTGGCCTCATGTG
  TCCGGAGGGTGGTGCCGGCGGTTCCGGGGCTTTTTCTGGTCCTGGGGCTCAGGAGACCGG
  GGTGCCCCTGGAGCCTCCCTGGGGCTGGGGGGCACATGGCGCCAGTAACCCTGGCAGTAC
  TGGTGGATGAGGCCCACTTCTGGCTGGGCCAGCAGCTGGGCTAACTCCTGGTAAGGAGGC
  GTTGGGGGGCCTGCGCCTGGGGGTGGCGGGGCGCTCATGGACAGTGGTGGGAAGAGGGCA
  GCATGGACGGCGTCCCGGCCCAGTACATGCAGCTGCACTCGTGTGACGACGTGCTTAAAG
  TTGTTCTCAGTGGCTGTGCAGGAGTAGAGGCCACGATCGCTGAGCTGCAGTGCACGGAGC
  AACAAGCCCTGCTCTGTGCGCAGGAAGCGGTCCTCTGCACGAATCTCTCGGCGCCGGTCA
  CCAGGATCTCGCTGGAACAGCCACTTAACAGTGGCTTGGGGCGAGCGGNGCTGGCACTCA
  AGGAAGCTGCGCTNGCCGCCACGCCATACTGCACAGACTNCACGGCATTCTTGTTGGCAT
  TNGGAGTGAACCCCACGCACTGCCTGATGGGTTTTCGTGCCGGAGTNCTGNCGCNNNGCT
  CGCTCTGNAGGAGCTGATAGNGGAGCAGCCTGCCATCCCAGCCAGTAGGGTCCCGGGCAG
  CANAGTCACACAGCAGCCCCTACNCTGCNANGTGCAGCTCAGTGTTGACACCCGCTGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004186
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004186.2, NP_004177.2
• RefSeq Size: 3535 bp
• RefSeq ORF: 2358 bp
• Locus ID: 6405
• Cytogenetics: 3p21.31
• Domains: Sema, IG, PSI
• Protein Families: Secreted Protein
• Protein Pathways: Axon guidance
• Gene Summary: 'This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin loop and a C-terminal basic domain. This gene is expressed by the endothelial cells where it was found to act in an autocrine fashion to induce apoptosis, inhibit cell proliferation and survival, and function as an anti-tumorigenic agent. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).