CDH12 (NM_004061) Human Untagged Clone

CAT#: SC117606

CDH12 (untagged)-Human cadherin 12, type 2 (N-cadherin 2) (CDH12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDH12
• Synonyms: CDHB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004061, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTTACAAGGAACTGTTTATCCCTGCTTCTCTGGGTTCTGTTTGATGGAGGTCTCCTAACACCACTAC
  AACCACAGCCACAGCAGACTTTAGCCACAGAGCCAAGAGAAAATGTTATCCATCTGCCAGGACAACGGTC
  ACATTTCCAACGTGTTAAACGTGGCTGGGTATGGAATCAATTTTTTGTGCTGGAAGAATACGTGGGCTCC
  GAGCCTCAGTATGTGGGAAAGCTCCATTCCGACTTAGACAAGGGAGAGGGCACTGTGAAATACACCCTCT
  CAGGAGATGGCGCTGGCACCGTTTTTACCATTGATGAAACCACAGGGGACATTCATGCAATAAGGAGCCT
  AGATAGAGAAGAGAAACCTTTCTACACTCTTCGTGCTCAGGCTGTGGACATAGAAACCAGAAAGCCCCTG
  GAGCCTGAATCAGAATTCATCATCAAAGTGCAGGATATTAATGATAATGAGCCAAAGTTTTTGGATGGAC
  CTTATGTTGCTACTGTTCCAGAAATGTCTCCTGTGGGTGCATATGTACTCCAGGTCAAGGCCACAGATGC
  AGATGACCCGACCTATGGAAACAGTGCCAGAGTCGTTTACAGCATTCTTCAGGGACAACCTTATTTCTCT
  ATTGATCCCAAGACAGGTGTTATTAGAACAGCTTTGCCAAACATGGACAGAGAAGTCAAAGAACAATATC
  AAGTACTCATCCAAGCCAAGGATATGGGAGGACAGCTTGGAGGATTAGCCGGAACAACAATAGTCAACAT
  CACTCTCACCGATGTCAATGACAATCCACCTCGATTCCCCAAAAGCATCTTCCACTTGAAAGTTCCTGAG
  TCTTCCCCTATTGGTTCAGCTATTGGAAGAATAAGAGCTGTGGATCCTGATTTTGGACAAAATGCAGAAA
  TTGAATACAATATTGTTCCAGGAGATGGGGGAAATTTGTTTGACATCGTCACAGATGAGGATACACAAGA
  GGGAGTCATCAAATTGAAAAAGCCTTTAGATTTTGAAACAAAGAAGGCATACACTTTCAAAGTTGAGGCT
  TCCAACCTTCACCTTGACCACCGGTTTCACTCGGCGGGCCCTTTCAAAGACACAGCTACGGTGAAGATCA
  GCGTGCTGGACGTAGATGAGCCACCGGTTTTCAGCAAGCCGCTCTACACCATGGAGGTTTATGAAGACAC
  TCCGGTAGGGACCATCATTGGCGCTGTCACTGCTCAAGACCTGGATGTAGGCAGCAGTGCTGTTAGGTAC
  TTCATAGATTGGAAGAGTGATGGGGACAGCTACTTTACAATAGATGGAAATGAAGGAACCATCGCCACTA
  ATGAATTACTAGACAGAGAAAGCACTGCGCAGTATAATTTCTCCATAATTGCGAGTAAAGTTAGTAACCC
  TTTATTGACCAGCAAAGTCAATATACTGATTAATGTCTTAGATGTAAATGAATTTCCTCCAGAAATATCT
  GTGCCATATGAGACAGCCGTGTGTGAAAATGCCAAGCCAGGACAGATAATTCAGATAGTCAGTGCTGCAG
  ACCGAGATCTTTCACCTGCTGGGCAACAATTCTCCTTTAGATTATCACCTGAGGCTGCTATCAAACCAAA
  TTTTACAGTTCGTGACTTCAGAAACAACACAGCGGGGATTGAAACCCGAAGAAATGGATACAGCCGCAGG
  CAGCAAGAGTTGTATTTCCTCCCTGTTGTAATAGAAGACAGCAGCTACCCTGTCCAGAGCAGCACAAACA
  CAATGACTATTCGAGTCTGTAGATGTGACTCTGATGGCACCATCCTGTCTTGTAATGTGGAAGCAATTTT
  TCTACCTGTAGGACTTAGCACTGGGGCGTTGATTGCAATTCTACTATGCATTGTTATACTCTTAGCCATA
  GTTGTACTGTATGTAGCACTGCGAAGGCAGAAGAAAAAAGACACCCTGATGACCTCTAAAGAAGACATCA
  GAGACAACGTCATCCATTACGATGATGAAGGAGGTGGGGAGGAAGATACCCAGGCTTTCGACATCGGGGC
  TCTGAGAAACCCAAAAGTGATTGAGGAGAACAAAATTCGCAGGGATATAAAACCAGACTCTCTCTGTTTA
  CCTCGTCAGAGACCACCCATGGAAGATAACACAGACATAAGGGATTTCATTCATCAAAGGCTACAGGAAA
  ATGATGTGGATCCAACTGCCCCACCATACGATTCACTGGCCACATATGCCTACGAAGGGAGTGGGTCCGT
  GGCAGAGTCCCTCAGCTCTATAGACTCTCTCACCACAGAAGCCGACCAGGACTATGACTATCTGACAGAC
  TGGGGACCCCGCTTTAAAGTCTTGGCAGACATGTTTGGCGAAGAAGAGAGTTATAACCCTGATAAAGTCA
  CTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004061 unedited
  NGGGTCATATATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCTTTA
  TCCGGGTGACTGCATGGCACTGAATTTCTGCATTGAAAGTTCTAACTGACTAAACTGACA
  TTCTGCACTGCAGCAAGTGACTGTGTCAGGAAAAGCTTAATTTATAAGCAAGTTTCCTGG
  AGTGAAAACGGCGTTGGAGATAATGATATCTCAGGGCAATTTCTTCTGAAATACATTCAT
  TCTGCCTTGTATCCAGAGAAAATAAAAGGGAACATGAAAAGATAGTTAATTTCTCATTTT
  ACCTTTTTAATAATCTATTTGGAGAGTTGTGCATTGCCAAGGCCCCTTTAGATGGAATAT
  AATACAGCAGAAGCCTTTTCATCAAAAGGATTAATGCCTGCATCATCATTTCTGCAATAA
  AACAAGAAGCTAAGTGGTGGAGGCCACAGACACCTCAAACCTGGATTCCACAATTCTACG
  TTAAGTGTTGGAGTTTTTATTACTCTGCTGTAGGAAAGCCTTTGCCAATGCTTACAAGGA
  ACTGTTTATCCCTGCTTCTCTGGGTTCTGTTTGATGGAGGTCTCCTAACACCACTACAAC
  CACAGCCACAGCAGACTTTAGCCACAGAGCCAAGAGAAAATGTTATCCATCTGCCAGGAC
  AACGGTCACATTTCCAACGTGTTAAACGTGGCTGGGTATGGAATCAATTTTTTGTGCTGG
  AAAGATACGTGGGCTCCGAGCCTCAGTATGTGGGAAAGCTCCATTCCGACTTAGACAAGG
  NAGAGGNCACTGTGAAATACACCCTCTCCAGAGATGCGCTGGCACCGTTTTTACATTGAT
  GAAACACAGGGGACATTCATGCCATTAAGAGCCTAGATAGAGAAGAGAAACTTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004061 unedited
  GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTTCTTTTTTTTTTGGAAAAGAAAG
  TAACATTTTAATGAACAGCTTTAATGTGAACTAATGTTAAGATCAAACATTGTTTCTAGA
  ATTTGGGACTTACATATTGAACTTGATATTTTCATATTTATTTCAATTTTCTGAAAGGAT
  AGGATAAACAAATACGTTTTAGCATACAGTTTAAAAAAGAAAAAGAAAACCTTTTGAATT
  AGAAGAAGGTGGAGATCAGAAAGTGTTCTTCTTTTGTTCTCAAAAGGAATGAGGAGAGTC
  AGAGTGTCGCTATTGCTTCTTGACATAAAGACGGAGTCTGTTTTATGTCTTTATCTCTGA
  ATCCCGCAATACCATCACAGCAGAGTGTACTGTACACCACTTTAAATGATAAGAGTTTCA
  ATAGGCACTTTAGTGGAATTAAGAAAAATCTAACACTCTTCCACCGTGATGCCACATAGC
  TATCTTCGTTCTAGGTTGTCATGTCAGTAAATTGTATTGAATAGGCCTGAGCTTGTCTCA
  GTGTGATTGTGAAAAAACAAAACAACAAAACAAAGCAAGTCACATTATAGGATGTATCTC
  TTGTTGGCAGAATAAACCAAAACTGACACTATGATTAATTTAGTAACTTACTAGAAACCA
  GGTAATCAAAGGAATCTTGTCCCAAAANAATGTGTGTGTGTGTGTGTGTGTTTGTGTGTG
  TGTGTGTGTGTGTGTGTGTCTAAAAAAAATTTCTATTAATATTTGGGGTTTCTTTTCCTT
  TTTAAAAAACCTCCCTCTCGGGTGTATTTAGCCTNCACGACTNCCTTAGTGACTTTACAA
  GGGTATTACTCTCTTCTTCGNCAACATGTCTGCCAAGACTTAAAGCGGGTCCCCAGTCTG
  CAGATATNCATAGCCTGGCCGGTTTGGGGGAGAAGAGTCTTAGAGCTGAGGACTCTGCAC
  GGACCACTCCCTTTAGCTATGTGGCATAATCNTTGTGGGGCATTGGCCCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004061
• Insert Size: 3850 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004061.2, NP_004052.2
• RefSeq Size: 4164 bp
• RefSeq ORF: 2385 bp
• Locus ID: 1010
• Cytogenetics: 5p14.3
• Domains: Cadherin_C_term, CA
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature cadherin protein. These integral membrane proteins mediate calcium-dependent cell-cell adhesion and are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1, 2, and 4-8 all encode the same isoform (1).