CDH12 (NM_004061) Human Untagged Clone

CAT#: SC117606

CDH12 (untagged)-Human cadherin 12, type 2 (N-cadherin 2) (CDH12)


  "NM_004061" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CDH12
Synonyms CDHB
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_004061, the custom clone sequence may differ by one or more nucleotides


ATGCTTACAAGGAACTGTTTATCCCTGCTTCTCTGGGTTCTGTTTGATGGAGGTCTCCTAACACCACTAC
AACCACAGCCACAGCAGACTTTAGCCACAGAGCCAAGAGAAAATGTTATCCATCTGCCAGGACAACGGTC
ACATTTCCAACGTGTTAAACGTGGCTGGGTATGGAATCAATTTTTTGTGCTGGAAGAATACGTGGGCTCC
GAGCCTCAGTATGTGGGAAAGCTCCATTCCGACTTAGACAAGGGAGAGGGCACTGTGAAATACACCCTCT
CAGGAGATGGCGCTGGCACCGTTTTTACCATTGATGAAACCACAGGGGACATTCATGCAATAAGGAGCCT
AGATAGAGAAGAGAAACCTTTCTACACTCTTCGTGCTCAGGCTGTGGACATAGAAACCAGAAAGCCCCTG
GAGCCTGAATCAGAATTCATCATCAAAGTGCAGGATATTAATGATAATGAGCCAAAGTTTTTGGATGGAC
CTTATGTTGCTACTGTTCCAGAAATGTCTCCTGTGGGTGCATATGTACTCCAGGTCAAGGCCACAGATGC
AGATGACCCGACCTATGGAAACAGTGCCAGAGTCGTTTACAGCATTCTTCAGGGACAACCTTATTTCTCT
ATTGATCCCAAGACAGGTGTTATTAGAACAGCTTTGCCAAACATGGACAGAGAAGTCAAAGAACAATATC
AAGTACTCATCCAAGCCAAGGATATGGGAGGACAGCTTGGAGGATTAGCCGGAACAACAATAGTCAACAT
CACTCTCACCGATGTCAATGACAATCCACCTCGATTCCCCAAAAGCATCTTCCACTTGAAAGTTCCTGAG
TCTTCCCCTATTGGTTCAGCTATTGGAAGAATAAGAGCTGTGGATCCTGATTTTGGACAAAATGCAGAAA
TTGAATACAATATTGTTCCAGGAGATGGGGGAAATTTGTTTGACATCGTCACAGATGAGGATACACAAGA
GGGAGTCATCAAATTGAAAAAGCCTTTAGATTTTGAAACAAAGAAGGCATACACTTTCAAAGTTGAGGCT
TCCAACCTTCACCTTGACCACCGGTTTCACTCGGCGGGCCCTTTCAAAGACACAGCTACGGTGAAGATCA
GCGTGCTGGACGTAGATGAGCCACCGGTTTTCAGCAAGCCGCTCTACACCATGGAGGTTTATGAAGACAC
TCCGGTAGGGACCATCATTGGCGCTGTCACTGCTCAAGACCTGGATGTAGGCAGCAGTGCTGTTAGGTAC
TTCATAGATTGGAAGAGTGATGGGGACAGCTACTTTACAATAGATGGAAATGAAGGAACCATCGCCACTA
ATGAATTACTAGACAGAGAAAGCACTGCGCAGTATAATTTCTCCATAATTGCGAGTAAAGTTAGTAACCC
TTTATTGACCAGCAAAGTCAATATACTGATTAATGTCTTAGATGTAAATGAATTTCCTCCAGAAATATCT
GTGCCATATGAGACAGCCGTGTGTGAAAATGCCAAGCCAGGACAGATAATTCAGATAGTCAGTGCTGCAG
ACCGAGATCTTTCACCTGCTGGGCAACAATTCTCCTTTAGATTATCACCTGAGGCTGCTATCAAACCAAA
TTTTACAGTTCGTGACTTCAGAAACAACACAGCGGGGATTGAAACCCGAAGAAATGGATACAGCCGCAGG
CAGCAAGAGTTGTATTTCCTCCCTGTTGTAATAGAAGACAGCAGCTACCCTGTCCAGAGCAGCACAAACA
CAATGACTATTCGAGTCTGTAGATGTGACTCTGATGGCACCATCCTGTCTTGTAATGTGGAAGCAATTTT
TCTACCTGTAGGACTTAGCACTGGGGCGTTGATTGCAATTCTACTATGCATTGTTATACTCTTAGCCATA
GTTGTACTGTATGTAGCACTGCGAAGGCAGAAGAAAAAAGACACCCTGATGACCTCTAAAGAAGACATCA
GAGACAACGTCATCCATTACGATGATGAAGGAGGTGGGGAGGAAGATACCCAGGCTTTCGACATCGGGGC
TCTGAGAAACCCAAAAGTGATTGAGGAGAACAAAATTCGCAGGGATATAAAACCAGACTCTCTCTGTTTA
CCTCGTCAGAGACCACCCATGGAAGATAACACAGACATAAGGGATTTCATTCATCAAAGGCTACAGGAAA
ATGATGTGGATCCAACTGCCCCACCATACGATTCACTGGCCACATATGCCTACGAAGGGAGTGGGTCCGT
GGCAGAGTCCCTCAGCTCTATAGACTCTCTCACCACAGAAGCCGACCAGGACTATGACTATCTGACAGAC
TGGGGACCCCGCTTTAAAGTCTTGGCAGACATGTTTGGCGAAGAAGAGAGTTATAACCCTGATAAAGTCA
CTTAA


>OriGene 5' read for NM_004061 unedited
NGGGTCATATATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCTTTA
TCCGGGTGACTGCATGGCACTGAATTTCTGCATTGAAAGTTCTAACTGACTAAACTGACA
TTCTGCACTGCAGCAAGTGACTGTGTCAGGAAAAGCTTAATTTATAAGCAAGTTTCCTGG
AGTGAAAACGGCGTTGGAGATAATGATATCTCAGGGCAATTTCTTCTGAAATACATTCAT
TCTGCCTTGTATCCAGAGAAAATAAAAGGGAACATGAAAAGATAGTTAATTTCTCATTTT
ACCTTTTTAATAATCTATTTGGAGAGTTGTGCATTGCCAAGGCCCCTTTAGATGGAATAT
AATACAGCAGAAGCCTTTTCATCAAAAGGATTAATGCCTGCATCATCATTTCTGCAATAA
AACAAGAAGCTAAGTGGTGGAGGCCACAGACACCTCAAACCTGGATTCCACAATTCTACG
TTAAGTGTTGGAGTTTTTATTACTCTGCTGTAGGAAAGCCTTTGCCAATGCTTACAAGGA
ACTGTTTATCCCTGCTTCTCTGGGTTCTGTTTGATGGAGGTCTCCTAACACCACTACAAC
CACAGCCACAGCAGACTTTAGCCACAGAGCCAAGAGAAAATGTTATCCATCTGCCAGGAC
AACGGTCACATTTCCAACGTGTTAAACGTGGCTGGGTATGGAATCAATTTTTTGTGCTGG
AAAGATACGTGGGCTCCGAGCCTCAGTATGTGGGAAAGCTCCATTCCGACTTAGACAAGG
NAGAGGNCACTGTGAAATACACCCTCTCCAGAGATGCGCTGGCACCGTTTTTACATTGAT
GAAACACAGGGGACATTCATGCCATTAAGAGCCTAGATAGAGAAGAGAAACTTCT
>OriGene 3' read for NM_004061 unedited
GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTTCTTTTTTTTTTGGAAAAGAAAG
TAACATTTTAATGAACAGCTTTAATGTGAACTAATGTTAAGATCAAACATTGTTTCTAGA
ATTTGGGACTTACATATTGAACTTGATATTTTCATATTTATTTCAATTTTCTGAAAGGAT
AGGATAAACAAATACGTTTTAGCATACAGTTTAAAAAAGAAAAAGAAAACCTTTTGAATT
AGAAGAAGGTGGAGATCAGAAAGTGTTCTTCTTTTGTTCTCAAAAGGAATGAGGAGAGTC
AGAGTGTCGCTATTGCTTCTTGACATAAAGACGGAGTCTGTTTTATGTCTTTATCTCTGA
ATCCCGCAATACCATCACAGCAGAGTGTACTGTACACCACTTTAAATGATAAGAGTTTCA
ATAGGCACTTTAGTGGAATTAAGAAAAATCTAACACTCTTCCACCGTGATGCCACATAGC
TATCTTCGTTCTAGGTTGTCATGTCAGTAAATTGTATTGAATAGGCCTGAGCTTGTCTCA
GTGTGATTGTGAAAAAACAAAACAACAAAACAAAGCAAGTCACATTATAGGATGTATCTC
TTGTTGGCAGAATAAACCAAAACTGACACTATGATTAATTTAGTAACTTACTAGAAACCA
GGTAATCAAAGGAATCTTGTCCCAAAANAATGTGTGTGTGTGTGTGTGTGTTTGTGTGTG
TGTGTGTGTGTGTGTGTGTCTAAAAAAAATTTCTATTAATATTTGGGGTTTCTTTTCCTT
TTTAAAAAACCTCCCTCTCGGGTGTATTTAGCCTNCACGACTNCCTTAGTGACTTTACAA
GGGTATTACTCTCTTCTTCGNCAACATGTCTGCCAAGACTTAAAGCGGGTCCCCAGTCTG
CAGATATNCATAGCCTGGCCGGTTTGGGGGAGAAGAGTCTTAGAGCTGAGGACTCTGCAC
GGACCACTCCCTTTAGCTATGTGGCATAATCNTTGTGGGGCATTGGCCCCTA
Restriction Sites NotI-NotI     
ACCN NM_004061
Insert Size 3850 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004061.2, NP_004052.2
RefSeq Size 4164 bp
RefSeq ORF 2385 bp
Locus ID 1010
Cytogenetics 5p14.3
Domains Cadherin_C_term, CA
Protein Families Transmembrane
Gene Summary 'This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature cadherin protein. These integral membrane proteins mediate calcium-dependent cell-cell adhesion and are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. [provided by RefSeq, Nov 2015]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1, 2, and 4-8 all encode the same isoform (1).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.