AP2M1 (NM_004068) Human Untagged Clone

CAT#: SC117611

AP2M1 (untagged)-Human adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP2M1
• Synonyms: AP50; CLAPM1; mu2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117611 sequence for NM_004068 edited (data generated by NextGen Sequencing)
  ATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTCATCTCCCGAGTCTAC
  CGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAATGTTATCCATGCCCGG
  CAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTCTTCCACGTTAAGCGG
  TCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCTGCCATGGTCTTCGAA
  TTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAGATCAGCGAGGAAAAC
  ATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATTCTAGACTTTGGCTAC
  CCACAGAATTCCGAGACAGGCGCGCTGAAAACCTTCATCACGCAGCAGGGCATCAAGAGT
  CAGCATCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGGCAGATTGGC
  TGGCGGCGAGAGGGTATCAAGTATCGTCGGAATGAGCTCTTCCTGGATGTGCTGGAGAGT
  GTGAACCTGCTCATGTCCCCACAAGGGCAGGTGCTGAGTGCCCATGTGTCGGGCCGGGTG
  GTGATGAAGAGCTACCTGAGTGGCATGCCTGAATGCAAGTTTGGGATGAATGACAAGATT
  GTTATTGAAAAGCAGGGCAAAGGCACAGCTGATGAAACAAGCAAGAGCGGGAAGCAATCA
  ATTGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGCAAGTTTGACTCTGAA
  CGCAGCATCAGCTTTATCCCGCCAGATGGAGAGTTTGAGCTTATGAGGTATCGCACAACC
  AAGGACATCATCCTTCCCTTCCGGGTGATCCCGCTAGTGCGAGAAGTGGGACGCACCAAA
  CTGGAGGTCAAGGTGGTCATCAAGTCCAACTTTAAACCCTCACTGCTGGCTCAGAAGATC
  GAGGTGAGGATCCCAACCCCACTGAACACAAGCGGGGTGCAGGTGATCTGCATGAAGGGG
  AAGGCCAAGTACAAGGCCAGCGAGAATGCCATCGTGTGGAAGATCAAGCGCATGGCAGGC
  ATGAAGGAATCGCAGATCAGCGCAGAGATTGAGCTTCTGCCTACCAACGACAAGAAGAAA
  TGGGCTCGACCCCCCATTTCCATGAACTTTGAGGTGCCATTCGCGCCCTCTGGCCTCAAG
  GTGCGCTACTTGAAGGTGTTTGAACCGAAGCTGAACTACAGCGACCATGATGTCATCAAA
  TGGGTGCGCTACATTGGCCGCAGTGGCATTTATGAAACTCGCTGCTAG
  
  Clone variation with respect to NM_004068.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004068 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGAGGCAGCGGGCAGACGAG
  CAGGGGGCGGGCGGACATCTTGGGATCCGGAGAGTGGCCGGGCCGGCAGAGCAGGGGGCC
  GAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGAGACTGATCTGCCGCCATGATT
  GGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTCATCTCCCGAGTCTACCGAGAT
  GACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAATGTTATCCATGCCCGGCAGCAG
  GTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTCTTCCACGTTAAGCGGTCCAAC
  ATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCTGCCATGGTCTTCGAATTCCTC
  TATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAGATCAGCGAGGAAAACATCAAG
  AACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATTCTAGACTTTGGCTACCCACAG
  AATTCCGAGACAGGCGCGCTGAAAACCTTCATCACGCAGCAGGGCATCAAGAGTCAGCAT
  CAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGGCAGATTGGCTGGCGG
  CGAGAGGGTATCAAGTATCGTCGGAATGAGCTCTTCCTGGATGTGCTGGAGAGTGTGAAC
  CTGCTCATGTCCCCACAGGGGCAGGTGCTGAGTGCCCATGTGTCGGGCCGGGTGGTGATG
  AANAGCTACCTGAGTGGCATGCCTGAAATGCAGTTTGGGATGAAATGACAAGATTGTTAT
  TGAAAAGCAGGNCANAGGCACAGCTGATGAAACAAAGCAGAGCGGAAGCATCAATTGCAT
  TGTGACTGCACCTTCACCATGTGTGCGATCANCAGTTGACTCGACGCACATAGCTTTATC
  CGCGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004068 unedited
  TTTTTTTTCTTCCACACCTTCTACACAAATTTTATTGGGTTTGTAACTGGACGAAGTCAC
  ACGTGTACAAAATGAAGCTCACACTATCCCAAAGCAGAAGNTAGGGACTGAGGCCGGGGA
  TCCATTACTGGCCTTTGGGGGGAGCTCTGAGGTGAGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004068
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004068.3, NP_004059.2
• RefSeq Size: 1958 bp
• RefSeq ORF: 1308 bp
• Locus ID: 1173
• Cytogenetics: 3q27.1
• Domains: Adap_comp_sub
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Huntington's disease
• Gene Summary: 'This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]'
  Transcript Variant: This variant (1) lacks two alternate in-frame exons compared to variant 3. The resulting isoform (a) has the same N- and C-termini but is shorter compared to isoform c.