B4GALT3 (B4GALT2) (NM_003780) Human Untagged Clone

CAT#: SC117761

B4GALT2 (untagged)-Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2 (B4GALT2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: B4GALT2
• Synonyms: B4Gal-T2; B4Gal-T3; beta4Gal-T2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117761 sequence for NM_003780 edited (data generated by NextGen Sequencing)
  ATGAGCAGACTGCTGGGGGGGACGCTGGAGCGCGTCTGCAAGGCTGTGCTCCTTCTCTGC
  CTGCTGCACTTCCTCGTGGCCGTCATCCTCTACTTTGACGTCTACGCCCAGCACCTGGCC
  TTCTTCAGCCGCTTCAGTGCCCGAGGCCCTGCCCATGCCCTCCACCCAGCTGCTAGCAGC
  AGCAGCAGCAGCAGCAACTGCTCCCGGCCCAACGCCACCGCCTCTAGCTCCGGGCTCCCT
  GAGGTCCCCAGTGCCCTGCCCGGTCCCACGGCTCCCACGCTGCCACCCTGTCCTGACTCG
  CCACCTGGTCTTGTGGGCAGACTGCTGATCGAGTTCACCTCACCCATGCCCCTGGAGCGG
  GTGCACAGGGAGAACCCAGGCGTGCTCATGGGCGGCCGATACACACCGCCCGACTGCACC
  CCAGCCCAGACGGTGGCGGTCATCATCCCCTTTAGACACCGGGAACACCACCTGCGCTAC
  TGGCTCCACTATCTACACCCCATCTTGAGGCGGCAGCGGCTGCGCTACGGCGTCTATGTC
  ATCAACCAGCATGGTGAGGACACCTTCAACCGGGCCAAGCTGCTTAACGTGGGCTTCCTA
  GAGGCGCTGAAGGAGGATGCCGCCTATGACTGCTTCATCTTCAGCGATGTGGACCTGGTC
  CCCATGGATGACCGCAACCTATACCGCTGCGGCGACCAACCCCGCCACTTTGCCATTGCC
  ATGGACAAGTTTGGCTTCCGGCTTCCCTATGCTGGCTACTTTGGAGGTGTGTCAGGCCTG
  AGTAAGGCTCAGTTTCTGAGAATCAATGGCTTCCCCAATGAGTACTGGGGCTGGGGTGGC
  GAGGATGATGACATCTTCAACCGGATCTCCCTGACTGGGATGAAGATCTCACGCCCAGAC
  ATCCGAATTGGCCGCTACCGCATGATCAAGCACGACCGCGACAAGCATAACGAACCTAAC
  CCTCAGAGGTTTACCAAGATTCAAAACACGAAGCTGACCATGAAGCGGGACGGCATTGGG
  TCAGTGCGGTACCAGGTCTTGGAGGTGTCTCGGCAACCACTCTTCACCAATATCACAGTG
  GACATTGGGCGGCCTCCGTCGTGGCCCCCTCGGGGCTGA
  
  Clone variation with respect to NM_003780.4
  366 g=>c;909 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003780 unedited
  TTGTATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCCGCCGGAGCCGG
  GAGCCCTGCCCAAGTCGGAGCGGCGTCCCCTGCTGAGCCCCGAGCGCCGGGCCAGCAGCC
  GGATGCCCGGGCCCACTGGGCGGGCCAGTGGCCGCCTGCGGGATGAGCAGACTGCTGGGG
  GGGACGCTGGAGCGCGTCTGCAAGGCTGTGCTCCTTCTCTGCCTGCTGCACTTCCTCGTG
  GCCGTCATCCTCTACTTTGACGTCTACGCCCAGCACCTGGCCTTCTTCAGCCGCTTCAGT
  GCCCGAGGCCCTGCCCATGCCCTCCACCCAGCTGCTAGCAGCAGCAGCAGCAGCAGCAAC
  TGCTCCCGGCCCAACGCCACCGCCTCTAGCTCCGGGCTCCCTGAGGTCCCCAGTGCCCTG
  CCCGGTCCCACGGCTCCCACGCTGCCACCCTGTCCTGACTCGCCACCTGGTCTTGTGGGC
  AGACTGCTGATCGAGTTCACCTCACCCATGCCCCTGGAGCGGGTGCACAGGGAGAACCCA
  GGCGTGCTCATGGGCGGCCGATACACACCGCCCGACTGCACCCCAGCCCAGACGGTGGCG
  GTCATCATCCCCTTTAGACACCGGGAACACCACCTGCGCTACTGGCTCCACTATCTACAC
  CCCATCTTGAGGCGGCAGCGGCTGCGCTACGGCGTCTATGTCATCAACCAGCATGGTGAG
  GACACCTTTCACCGGGCCAAGCTGCTTAACGTGGGCTTCCTANAGGCGCTGAAGGGAGAT
  GCCGNCTATGACTGCTTCATCTTCAGCGATGTGGACCTGGTCCCCATGGATGNNACGCAC
  CTATACCGCTGCGGCGACCAACCCCGCCACTTTGCCATTGNCATGGNACAGTTNGGNCTT
  NCGGCTTNCCTCATGCTGCTACNTTGGNNAGGGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003780 unedited
  TTTTTTACTACANCACCTCCACAAGTTTCTGTGCAATGTACAAACACAAGCCGGCCCTGG
  GGCTCCTGCCTCCTCTCTCAGACAGGGAGGCGGGAACCAGACTTACTGATCCAAGCCCCA
  CCTGCCTCCCATGCAAAGGCCAACCCCAAGATCCCAGGCAGCGACACAGCATCCCCTCTG
  GTCCCCTCCTGGGGCTGCTCCTCAGCAGGGGGCGCACCAAAGCTTGCCCCCTACAATGTT
  GAATGAAAAAGGTTTCCCCTCCCCTGGGAGCAAGGGGGTTCCCAAGAGCTGAGAATTCCC
  CCTGGAAGAGACACTGCCCCCTAGACATACCTCCCACCCTTCCAGGCCCTAGGGGACTCA
  AGGGGGGGAAACTGAGGCACAGAGAGGTGGAGTGACCTAGCCCAGGCCACTCTGCGAGGC
  AGTGCAGGGGCTGGCCCGACCCTGACAGTGACTGGGGCTGGGCTAGGGGGAGGGGGTCGG
  GAAGGAGGGTTGACTCCAAAGAGGGCCACACTTGAAGAGCCTGCCCAGGCAGGAGGCCCC
  CCAAAGTGGGTTCTGAAACTTCCAGGTGTAGCTAGGGGGCCTAGTGAAGACCCTTGGAAA
  ACAGAGCCCAGTCTCAGTCCTGGAGGTCCTCCACAGAGCAGCTGCCAGCCAGGCTGTGGT
  CAGTCCTCTGGCAGGCAATCTTCGGCACCGAGAGCCTCTGTCCATTAGTGTCAGCCCCGA
  GGGGGCCACGACGGAGGCCGNCCAATGTCCACTGTGATATTGGTGAAGAAGTGGTGCCGA
  GACACCTCCAAGACCTGGTACCGNACTGACCCAATGCCGTCCNGCTNCATGGGTCAGCTC
  GTGTTTNGAAATCTTGGTAACCCTCTGAGGNNTANGNTCGTTATGCTTGTCGCGGTCGTG
  CTTGATCCATGCGTAGCGGCCAATTCGGATGTCTGGCGTGAGATCTCCATCCATCAGGGA
  GACCCGNNTGAGATGTANATNCTNGCACCCAGCCCAGACTATTGGGAAGCCATGATCTAA
  ACTGAGCTACTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003780
• ORF Size: 1119 bp
• Insert Size: 2000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_003780.3, NP_003771.1
• RefSeq Size: 2227
• RefSeq ORF: 1119
• Locus ID: 8704
• Domains: Galactosyl_T_2
• Protein Families: Transmembrane
• Protein Pathways: Galactose metabolism, Glycosphingolipid biosynthesis - lacto and neolacto series, Keratan sulfate biosynthesis, Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode isoform b.