CHIT1 (NM_003465) Human Untagged Clone

CAT#: SC117950

CHIT1 (untagged)-Human chitinase 1 (chitotriosidase) (CHIT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHIT1
• Synonyms: CHI3; CHIT; CHITD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117950 sequence for NM_003465 edited (data generated by NextGen Sequencing)
  ATGGTGCGGTCTGTGGCCTGGGCAGGTTTCATGGTCCTGCTGATGATCCCATGGGGCTCT
  GCTGCAAAACTGGTCTGCTACTTCACCAACTGGGCCCAGTACAGACAGGGGGAGGCTCGC
  TTCCTGCCCAAGGACTTGGACCCCAGCCTTTGCACCCACCTCATCTACGCCTTCGCTGGC
  ATGACCAACCACCAGCTGAGCACCACTGAGTGGAATGACGAGACTCTCTACCAGGAGTTC
  AATGGCCTGAAGAAGATGAATCCCAAGCTGAAGACCCTGTTAGCCATCGGAGGCTGGAAT
  TTCGGCACTCAGAAGTTCACAGATATGGTAGCCACGGCCAACAACCGTCAGACCTTTGTC
  AACTCGGCCATCAGGTTTCTGCGCAAATACAGCTTTGACGGCCTTGACCTTGACTGGGAG
  TACCCAGGAAGCCAGGGGAGCCCTGCCGTAGACAAGGAGCGCTTCACAACCCTGGTACAG
  GACTTGGCCAATGCCTTCCAGCAGGAAGCCCAGACCTCAGGGAAGGAACGCCTTCTTCTG
  AGTGCAGCGGTTCCAGCTGGGCAGACCTATGTGGATGCTGGATACGAGGTGGACAAAATC
  GCCCAGAACCTGGATTTTGTCAACCTTATGGCCTACGACTTCCATGGCTCTTGGGAGAAG
  GTCACGGGACATAACAGCCCCCTCTACAAGAGGCAAGAAGAGAGTGGTGCAGCAGCCAGC
  CTCAACGTGGATGCTGCTGTGCAACAGTGGCTGCAGAAGGGGACCCCTGCCAGCAAGCTG
  ATCCTTGGCATGCCTACCTACGGACGCTCCTTCACACTGGCCTCCTCATCAGACACCAGA
  GTGGGGGCCCCAGCCACAGGGTCTGGCACTCCAGGCCCCTTCACCAAGGAAGGAGGGATG
  CTGGCCTACTATGAAGTCTGCTCCTGGAAGGGGGCCACCAAACAGAGAATCCAGGATCAG
  AAGGTGCCCTACATCTTCCGGGACAACCAGTGGGTGGGCTTTGATGATGTGGAGAGCTTC
  AAAACCAAGGTCAGCTATCTGAAGCAGAAGGGACTGGGCGGGGCCATGGTCTGGGCACTG
  GACTTAGATGACTTTGCCGGCTTCTCCTGCAACCAGGGCCGATACCCCCTCATCCAGACG
  CTACGGCAGGAACTGAGTCTTCCATACTTGCCTTCAGGCACCCCAGAGCTTGAAGTTCCA
  AAACCAGGTCAGCCCTCTGAACCTGAGCATGGCCCCAGCCCTGGACAAGACACGTTCTGC
  CAGGGCAAAGCTGATGGGCTCTATCCCAATCCTCGGGAACGGTCCAGCTTCTACAGCTGT
  GCAGCGGGGCGGCTGTTCCAGCAAAGCTGCCCGACAGGCCTGGTGTTCAGCAACTCCTGC
  AAATGCTGCACCTGGAATTGA
  
  Clone variation with respect to NM_003465.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003465 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCTGAGCTGCATCATGGT
  GCGGTCTGTGGCCTGGGCAGGTTTCATGGTCCTGCTGAGATCCCTGGGGCTCTGCTGCAA
  AACTGGTCTGCTACTTCACCAACTGGGCCCAGTACAGACAGGGGGAGGCTCGCTTCCTGC
  CCAAGGACTTGGACCCCAGCCTTTGCACCCACCTCATCTACGCCTTCGCTGGCATGACCA
  ACCACCAGCTGAGCACCACTGAGTGGAATGACGAGACTCTCTACCAGGAGTTCAATGGCC
  TGAAGAAGATGAATCCCAAGCTGAAGACCCTGTTAGCCATCGGAGGCTGGAATTTCGGCA
  CTCAGAAGTTCACAGATATGGTAGCCACGGCCAACAACCGTCAGACCTTTGTCAACTCGG
  CCATCAGGTTTCTGCGCAAATACAGCTTTGACGGCCTTGACCTTGACTGGGAGTACCCAG
  GAAGCCAGGGGAGCCCTGCCGTAGACAAGGAGCGCTTCACAACCCTGGTACAGGACTTGG
  CCAATGCCTTCCAGCAGGAAGCCCAGACCTCAGGGAAGGAACGCCTTCTTCTGAGTGCAG
  CGGTTCCAGCTGGGCAGACCTATGTGGATGCTGGATACGAGGTGGACANAATCGCCCAGA
  ACCTGNATTNTGTCAACCTTATGGCCTACGACTTCCATGGGGCTCTGGAGAAGTCACGGG
  ACATAACAGCCCCCTCTACAAAGAGCAGAAGAGAGTGTGCAGCAGCCAGCCTCACGNTGA
  TGCTGCTGTGCACAGTGGCTGCAAAAGGGGACCCCTGNNCACAGCTGATCCTTGCATGCC
  TACTACNGACGCTCTTCAACTGGNCTNCTATCAACACCAAAGTGGGGGCCCAGCCACAGG
  TCTGCACTTNCAGCCCTTCACANGNANNGAGGNAGCTGNCTACCTATGAACTGCTCTGAA
  GGGGNNCACAACAAGAATCAGATCGAAGTGCCTAACTCTCGAAACCATNGNTGGCTTGTA
  ATGNGAAGCTCAANCGGTCACTTTGACAAGGATGGCGGGCTGNTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003465 unedited
  GTCGAGTTAAATCCTTTTTTTTTTTGAAGAGGGGCACAAACCAAAGATTTATTTTGCAAG
  TGAAAGGGGCAGCCCAGGAGACCCAGAAAAAAGGAAGGCAAGGCTGAGAGCAGAAAGCCT
  GGATAAAGGAAGACCACAGAAAGGCCTGCAGGAGCCAGATTGCGGCCCCCAGGGAAAACC
  CAGGAGGCAGGCTGTAGAGTGATCCTGGGCCCAGCCTCAAAGCTGGGACTGGAGGGGCTT
  TAGCGACTCAATTCCAGGTGCAGCATTTGCAGGAGTTGCTGAACACCAGGCCTGTCGGGC
  AGCTTTGCTGGAACAGCCGCCCCGCTGCACAGCTGTAGAAGCTGGACCGTTCCCGAGGAT
  TGGGATAGAGCCCATCAGCTTTGCCCTGGCAGAACGTGTCTTGTCCAGGGCTGGGGCCAT
  GCTCAGGTTCAGAGGGCTGACCTGGTTTTGGAACTTCAAGCTCTGGGGTGCCTGAAGGCA
  AGTATGGAAGACTCAGTTCCTGCCGTAGCGTCTGGATGAGGGGGTATCGGCCCTGGTTGC
  AGGAGAAGCCGGCAAAGTCATCTAAGTCCAGTGCCCAGACCATGGCCCCGCCCAGTCCCT
  TCTGCTTCAGATAGCTGACCTTGGTTTTGAAGCTCTCCACATCATCAAAGCCCACCCACT
  GGTTGTCCCGGAAGATGTANGGCACCTTCTGATCCTGGATTCTCTGTTTGGTGGCCCCCT
  TCCAGGAGCAGACTTCATAGTAGGCCAGCATCCCTCCTTCCTTGGTGAAGGGGCCTGGNA
  GTGCAGACCCTGTGGCTGGGGCCCCCACTCTGGTGTCTGATGAGGGAGCCAGTGTGAAAG
  AACCTCCGTTAGTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003465
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003465.1, NP_003456.1
• RefSeq Size: 1633 bp
• RefSeq ORF: 1401 bp
• Locus ID: 1118
• Cytogenetics: 1q32.1
• Domains: Glyco_18, CBM_14
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Amino sugar and nucleotide sugar metabolism
• Gene Summary: 'Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (1) is the predominant transcript and it encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. A downstream translational start codon is selected for this RefSeq based on its better conservation in mammalian species and on the presence of a predicted signal peptide in the protein N-terminus. An upstream in-frame start codon is also present but is poorly conserved, and use of the upstream start codon would result in a protein that is 10 aa longer at the N-terminus and lacks a predicted signal peptide. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon.