UBA52 (NM_003333) Human Untagged Clone

CAT#: SC118043

UBA52 (untagged)-Human ubiquitin A-52 residue ribosomal protein fusion product 1 (UBA52), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBA52
• Synonyms: CEP52; HUBCEP52; L40; RPL40
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118043 sequence for NM_003333 edited (data generated by NextGen Sequencing)
  ATGCAGATCTTTGTGAAGACCCTCACTGGCAAAACCATCACCCTTGAGGTCGAGCCCAGT
  GACACCATTGAGAATGTCAAAGCCAAAATTCAAGACAAGGAGGGTATCCCACCTGACCAG
  CAGCGTCTGATATTTGCCGGCAAACAGCTGGAGGATGGCCGCACTCTCTCAGACTACAAC
  ATCCAGAAAGAGTCCACCCTGCACCTGGTGTTGCGCCTGCGAGGTGGCATTATTGAGCCT
  TCTCTCCGCCAGCTTGCCCAGAAATACAACTGCGACAAGATGATCTGCCGCAAGTGCTAT
  GCTCGCCTTCACCCTCGTGCTGTCAACTGCCGCAAGAAGAAGTGTGGTCACACCAACAAC
  CTGCGTCCCAAGAAGAAGGTCAAATAA
  
  Clone variation with respect to NM_003333.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003333 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTGGTTGGTGG
  CGGCGGTCGTGCGGACGCAAACATGCAGATCTTTGTGAAGACCCTCACTGGCAAAACCAT
  CACCCTTGAGGTCGAGCCCAGTGACACCATTGAGAATGTCAAAGCCAAAATTCAAGACAA
  GGAGGGTATCCCACCTGACCAGCAGCGTCTGATATTTGCCGGCAAACAGCTGGAGGATGG
  CCGCACTCTCTCAGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGTTGCGCCT
  GCGAGGTGGCATTATTGAGCCTTCTCTCCGCCAGCTTGCCCAGAAATACAACTGCGACAA
  GATGATCTGCCGCAAGTGCTATGCTCGCCTTCACCCTCGTGCTGTCAACTGCCGCAAGAA
  GAAGTGTGGTCACACCAACAACCTGCGTCCCAAGAAGAAGGTCAAATAAGGTGGTTCTTT
  CCTTGAAGGGCAGCCTCCTGCCCAGGCCCCGTGGCCCTGGAGCCTCAATAAAGTGTCCCT
  TTCATTGACTGGAGCAGCAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGG
  TCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTC
  TCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTT
  GCATCATTTTGTCTGACTAGGTGTNCTTCTATATATTATGGGGTGGAGGGGGGTGGGTAT
  GGAGCAAGGGGCAAGNTGGGAAGACAACCTGTAGGNCCTGCCGNGTCTATTGGGAACCAA
  GCTGGAGTGCAGTGGCACAATCTTGGNCTCACTGCATCTCCGCCTNCTGGGTTCAAGCGA
  TTCTCCTGCCTCAGCCTCCCGAGTTGNTGGGATTCCAGGCATGCATGACCACGCTCAGCT
  ATTNTTGTTTTTTTGTAAAGACGGGGTTTACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003333 unedited
  GACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCTGCTCCAGTCAAT
  GAAAGGGACACTTTATTGAGGCTCCAGGGCCACGGGGCCTGGGCAGGAGGCTGCCCTTCA
  AGGAAAGAACCACCTTATTTGACCTTCTTCTTGGGACGCAGGTTGTTGGTGTGACCACAC
  TTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCGAGCATAGCACTTGCGGCAGATC
  ATCTTGTCGCAGTTGTATTTCTGGGCAAGCTGGCGGAGAGAAGGCTCAATAATGCCACCT
  CGCAGGCGCAACACCAGGTGCAGGGTGGACTCTTTCTGGATGTTGTAGTCTGAGAGAGTG
  CGGCCATCCTCCAGCTGTTTGCCGGCAAATATCAGACGCTGCTGGTCAGGTGGGATACCC
  TCCTTGTCTTGAATTTTGGCTTTGACATTCTCAATGGTGTCACTGGGCTCGACCTCAAGG
  GTGATGGTTTTGCCAGTGAGGGTCTTCACAAAGATCTGCATGTTTGCGTCCGCACGACCG
  CCGCCACCAACCAGCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAA
  AATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACCACGCCTA
  CCGCCCATTTGCGTCAACGGCGCCGGCGTTATTACGACATTTTGGAAAGTCCCCGTGATT
  TTGGTGCCAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGTAAATCCCCGTGAG
  TCAAACCGCTATCCACGCCCCATTGGTGTACTGGCCAAACCCGCATCACCATGGTTAATA
  GCGATGACCTATTCCCTACAATGTCCGCACAAGTAGCAAAGCCCCGTAAGGTCATGCACT
  GGGCATATTGCCAGCCGGCCATTTACCGTCTTGGACGTAATAGGGGGCGAACTTGCATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003333
• Insert Size: 550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003333.2, NP_003324.1
• RefSeq Size: 515 bp
• RefSeq ORF: 387 bp
• Locus ID: 7311
• Cytogenetics: 19p13.11
• Domains: UBQ, Ribosomal_L40e
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Ribosome
• Gene Summary: 'Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This predominant variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.