ALDOB (NM_000035) Human Untagged Clone

CAT#: SC118108

ALDOB (untagged)-Human aldolase B, fructose-bisphosphate (ALDOB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALDOB
• Synonyms: ALDB; ALDO2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118108 sequence for NM_000035 edited (data generated by NextGen Sequencing)
  ATGGCCCACCGATTTCCAGCCCTCACCCAGGAGCAGAAGAAGGAGCTCTCAGAAATTGCC
  CAGAGCATTGTTGCCAATGGAAAGGGGATCCTGGCTGCAGATGAATCTGTAGGTACCATG
  GGGAACCGCCTGCAGAGGATCAAGGTGGAAAACACTGAAGAGAACCGCCGGCAGTTCCGA
  GAAATCCTCTTCTCTGTGGACAGTTCCATCAACCAGAGCATCGGGGGTGTGATCCTTTTC
  CACGAGACCCTCTACCAGAAGGACAGCCAGGGAAAGCTGTTCAGAAACATCCTCAAGGAA
  AAGGGGATCGTGGTGGGAATCAAGTTAGACCAAGGAGGTGCTCCTCTTGCAGGAACAAAC
  AAAGAAACCACCATTCAAGGGCTTGATGGCCTCTCAGAGCGCTGTGCTCAGTACAAGAAA
  GATGGTGTTGACTTTGGGAAGTGGCGTGCTGTGCTGAGGATTGCCGACCAGTGTCCATCC
  AGCCTCGCTATCCAGGAAAACGCCAACGCCCTGGCTCGCTACGCCAGCATCTGTCAGCAG
  AATGGACTGGTACCTATTGTTGAACCAGAGGTAATTCCTGATGGAGACCATGACCTGGAA
  CACTGCCAGTATGTTACTGAGAAGGTCCTGGCTGCTGTCTACAAGGCCCTGAATGACCAT
  CATGTTTACCTGGAGGGCACCCTGCTAAAGCCCAACATGGTGACTGCTGGACATGCCTGC
  ACCAAGAAGTATACTCCAGAACAAGTAGCTATGGCCACCGTAACAGCTCTCCACCGTACT
  GTTCCTGCAGCTGTTCCTGGCATCTGCTTTTTGTCTGGTGGCATGAGTGAAGAGGATGCC
  ACTCTCAACCTCAATGCTATCAACCTTTGCCCTCTACCAAAGCCCTGGAAACTAAGTTTC
  TCTTATGGACGGGCCCTGCAGGCCAGTGCACTGGCTGCCTGGGGTGGCAAGGCTGCAAAC
  AAGGAGGCAACCCAGGAGGCTTTTATGAAGCGGGCCATGGCTAACTGCCAGGCGGCCAAA
  GGACAGTATGTTCATACGGGTTCTTCTGGGGCTGCTTCCACCCAGTCGCTCTTCACAGCC
  TGCTATACCTACTAG
  
  Clone variation with respect to NM_000035.3
  1035 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000035 unedited
  TCAGCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACCCAGGCCTCAC
  CCACAGCTTTTGATATCTAGGAGGACTCTTCTCTCCCAAACTACCTGTCACCATGGCCCA
  CCGATTTCCAGCCCTCACCCAGGAGCAGAAGAAGGAGCTCTCAGAAATTGCCCAGAGCAT
  TGTTGCCAATGGAAAGGGGATCCTGGCTGCAGATGAATCTGTAGGTACCATGGGGAACCG
  CCTGCAGAGGATCAAGGTGGAAAACACTGAAGAGAACCGCCGGCAGTTCCGAGAAATCCT
  CTTCTCTGTGGACAGTTCCATCAACCAGAGCATCGGGGGTGTGATCCTTTTCCACGAGAC
  CCTCTACCAGAAGGACAGCCAGGGAAAGCTGTTCAGAAACATCCTCAAGGAAAAGGGGAT
  CGTGGTGGGAATCAAGTTAGACCAAGGAGGTGCTCCTCTTGCAGGAACAAACAAAGAAAC
  CACCATTCAAGGGCTTGATGGCCTCTCAGAGCGCTGTGCTCAGTACAAGAAAGATGGTGT
  TGACTTTGGGAAGTGGCGTGCTGTGCTGAGGATTGCCGACCAGTGTCCATCCAGCCTCGC
  TATCCAGGAAAACGCCAACGCCCTGGCTCGCTACGCCAGCATCTGTCAGCAGAATGGACT
  GGTACCTATTGTTGAACCAGAGGTAATTCCTGATGGAGACCATGACCTGNAACACTGCCA
  GTATGTTACTGAGAAGGTCCTGGCTGCTGTCTACAANGCCCTGAATGACCATCATGTNNT
  ACCTGGAGGCACCCTGCTAAAGCCCACATGGTGACTGCTGGACATGCCTGCACCAAGAGT
  ATACTNCAGAACAGTAGCTAGGCCACCGTAACAGCTCNTCACGTACTGGTCCTGCAGCTG
  TNCCTGGCATCTGCTTTTTGTCTGGTGGCATGAGTGAAAAGGAGCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000035 unedited
  AACCTTGATAGGATATTTATTTCTCTTTGGTTGCAGCTATCTCCTTCCCAACCTACCACT
  GCTAATACTGTTTCATAAGATGCACTTGCTCTACACTCAGCTAATGAGGTGTTTCAATCA
  GCAGTTGTTCTTTGGATGAGGAGCCGATATTGTTTTAAAGCCTATTATTTTCTTGGGTGG
  GTATTCTGGAGCATGGGGAGCTGAAAGTGTTGCAAGGTATACTGCTGTGTGAAATTTGAT
  CAGGTGCGGGGGATTCATTTTTATGTTTCAATAACTGATTTATTTTTTCCCCCTTGTACT
  TAAGATTTAACATGTGTTGTATTTCCAGCAGTTCAAATCTAATTGTGTCGAATTTCCAGG
  ATTGGAGGAAAAGTTGCTCCCTTTCAGCCCTCCTACTAGAAGCACTGGAGCTAGGCTGGC
  GGGCATTGGACCCTAGTAGGTATAGCAGGCTGTGAAGAGCGACTGGGTGGAAGCAGCCCC
  AGAAGAACCCGTATGAACATACTGTCCTTTGGCCGCCTGGCAGTTAGCCATGGCCCGCTT
  CATAAAAGCCTCCTGGGTTGCCTCCTTGTTTGCAGCCTTGCCACCCCAGGCAGCCAGTGC
  ACTGGCCTGCAGGGCCCGTCCATAAGAGAAACTTAGTTTCCAGGGCTTTGGTAGAGGGCA
  AAGGTTGATAGCATTGAGGTTGAGAGTGGCATTCTCTTCACTCATGCCACCAGACAAAAA
  GCAGATGCCAAGAACAGCTGCAGGAACAGTACGGTGGAGAGCTGTTACCGTGGGCATAGC
  TACTTGTTCTTGAGTATACTTCTTGGGTGCAGGCATGTCCAGCAGTCACCATGTTTGGGC
  TTTACCAGGGTGCCCTTCCAGTTAACATGATGGGCATTCACGGCCCTGTAGACAGCAGCC
  CAGGACTTTTTAGTAACATCTGGGAAGTGTTCCAGTAATGGCCTA
  
• Restriction Sites: Please inquire
• ACCN: NM_000035
• Insert Size: 1680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000035.2, NP_000026.2
• RefSeq Size: 1669 bp
• RefSeq ORF: 1095 bp
• Locus ID: 229
• Cytogenetics: 9q31.1
• Domains: glycolytic_enzy
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway
• Gene Summary: 'Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]'