TAF2 (NM_003184) Human Untagged Clone

CAT#: SC118117

TAF2 (untagged)-Human TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (TAF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAF2
• Synonyms: CIF150; MRT40; TAF2B; TAFII150
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118117 sequence for NM_003184 edited (data generated by NextGen Sequencing)
  ATGCCGCTGACTGGTGTAGAGCCCGCCAGAATGAACAGGAAGAAAGGAGACAAGGGCTTT
  GAAAGCCCAAGGCCATATAAATTAACCCATCAGGTCGTCTGCATCAACAACATAAATTTC
  CAGAGAAAATCTGTTGTGGGATTTGTGGAACTGACTATATTTCCCACAGTTGCAAACTTG
  AATAGAATCAAGTTGAACAGCAAACAGTGTAGAATATACCGAGTAAGGATCAATGATTTA
  GAGGCTGCTTTTATTTATAATGACCCAACCTTGGAAGTTTGTCACAGTGAATCAAAACAG
  AGAAACCTCAATTATTTTTCCAATGCTTATGCAGCTGCAGTTAGTGCTGTGGACCCTGAT
  GCAGGAAATGGAGAACTTTGCATTAAGGTTCCATCAGAGCTATGGAAACACGTTGATGAG
  TTAAAGGTCCTGAAGATACACATCAATTTTTCTTTGGATCAGCCCAAAGGAGGTCTTCAT
  TTTGTGGTACCCAGTGTAGAGGGAAGTATGGCAGAGAGAGGTGCTCATGTTTTCTCTTGT
  GGGTATCAAAATTCTACAAGATTTTGGTTCCCTTGTGTTGATTCATACTCTGAATTGTGT
  ACATGGAAATTAGAATTTACAGTAGATGCTGCAATGGTTGCTGTTTCTAATGGCGATTTG
  GTGGAGACAGTGTATACTCATGATATGAGGAAGAAAACTTTCCATTATATGCTTACCATT
  CCTACAGCAGCGTCAAATATCTCCTTGGCCATTGGACCATTTGAAATACTGGTAGATCCA
  TACATGCATGAGGTTACTCATTTTTGTTTGCCCCAACTTCTTCCATTGCTGAAACATACC
  ACATCATACCTTCATGAAGTCTTTGAATTTTATGAAGAAATTCTTACATGTCGTTACCCA
  TACTCCTGTTTTAAGACTGTCTTCATTGATGAGGCTTATGTTGAAGTGGCTGCTTATGCT
  TCCATGAGCATTTTTAGCACAAATCTTTTACACAGTGCCATGATTATAGATGAGACACCT
  TTGACTAGAAGGTGTTTAGCCCAATCCTTGGCCCAGCAGTTTTTTGGTTGTTTCATATCT
  AGAATGTCTTGGTCTGATGAATGGGTGCTGAAGGGAATTTCAGGCTATATCTATGGACTT
  TGGATGAAAAAAACTTTTGGTGTTAATGAGTACCGCCATTGGATTAAAGAGGAGCTAGAC
  AAAATAGTGGCATATGAACTAAAAACTGGTGGGGTTTTACTACATCCCATATTTGGTGGA
  GGAAAAGAGAAGGATAATCCGGCTTCCCATCTACACTTTTCAATAAAGCATCCACATACA
  CTGTCCTGGGAATACTACACTATGTTTCAGTGTAAAGCCCACCTTGTGATGAGATTGATT
  GAAAATAGGATCAGTATGGAATTTATGCTACAAGTTTTCAATAAACTGCTAAGTCTGGCT
  AGTACTGCTTCATCTCAGAAGTTCCAGTCACATATGTGGAGTCAGATGTTGGTTTCCACA
  TCTGGGTTTTTGAAATCCATTTCAAATGTCTCTGGCAAAGATATTCAGCCGTTAATAAAG
  CAGTGGGTAGATCAGAGTGGAGTGGTAAAATTTTATGGAAGTTTTGCATTTAATAGAAAA
  CGAAATGTCTTGGAACTGGAAATAAAACAGGACTATACATCTCCTGGAACTCAGAAATAC
  GTGGGACCACTTAAAGTGACAGTGCAGGAGTTAGATGGATCCTTCAATCATACACTGCAA
  ATTGAAGAAAACAGCCTTAAACATGATATACCCTGCCATTCCAAAAGTAGAAGGAATAAA
  AAGAAAAAAATCCCACTGATGAATGGAGAAGAAGTTGATATGGATCTTTCTGCAATGGAT
  GCTGATTCCCCTTTGCTGTGGATAAGGATAGACCCAGATATGTCAGTATTGAGGAAGGTA
  GAATTTGAGCAAGCTGATTTTATGTGGCAGTATCAGCTCCGCTATGAGAGAGATGTTGTT
  GCACAGCAGGAATCCATTTTGGCTTTGGAAAAATTCCCTACTCCAGCATCTCGGCTTGCA
  CTCACTGATATATTAGAACAAGAGCAGTGTTTCTACAGAGTAAGAATGTCAGCTTGCTTC
  TGTCTTGCAAAGATTGCAAATTCAATGGTGAGCACATGGACAGGACCACCAGCCATGAAG
  TCACTCTTCACTAGGATGTTTTGTTGTAAAAGTTGTCCAAACATTGTGAAAACAAACAAC
  TTTATGAGCTTTCAAAGCTATTTTCTACAGAAGACTATGCCAGTTGCAATGGCTTTATTA
  AGAGATGTTCATAATCTTTGTCCTAAAGAAGTCTTAACGTTTATTTTAGACTTAATCAAG
  TACAATGACAACAGGAAAAATAAGTTTTCAGATAACTATTATCGTGCAGAAATGATTGAT
  GCCCTGGCCAACTCTGTTACACCTGCAGTCAGTGTGAATAATGAAGTTAGAACTTTGGAT
  AACTTAAATCCTGATGTGCGACTCATTCTTGAAGAAATCACCAGATTTTTGAATATGGAA
  AAACTTCTTCCGAGTTACAGGCATACCATCACTGTCAGTTGTTTGAGAGCCATACGGGTA
  CTTCAGAAGAACGGACATGTGCCAAGTGATCCAGCTCTTTTTAAATCTTATGCTGAATAT
  GGCCACTTTGTGGACATTAGGATAGCAGCTTTGGAAGCAGTTGTTGATTATACTAAAGTG
  GACAGAAGTTATGAAGAACTGCAATGGCTACTTAATATGATTCAGAATGACCCTGTACCC
  TATGTAAGGCATAAGATTCTCAACATGTTGACTAAGAACCCACCATTTACTAAGAACATG
  GAGTCTCCCTTATGCAATGAAGCCCTGGTAGATCAACTTTGGAAACTTATGAATTCTGGT
  ACTTCACATGACTGGAGGTTACGGTGTGGTGCTGTGGACTTGTACTTCACACTTTTTGGC
  CTCAGTAGACCTTCCTGTTTACCCTTGCCAGAGCTTGGGTTGGTTCTTAATCTAAAGGAG
  AAAAAAGCTGTCTTGAATCCTACCATAATTCCAGAGTCAGTAGCAGGCAACCAAGAAGCT
  GCAAATAATCCAAGCAGTCACCCACAGCTAGTTGGATTTCAGAACCCTTTTTCCAGTTCT
  CAAGATGAGGAGGAGATTGATATGGATACTGTTCATGATAGCCAGGCCTTCATTTCCCAT
  CATTTAAACATGCTTGAAAGGCCGTCAACTCCAGGGCTCTCGAAATATCGGCCAGCTAGC
  TCCCGATCTGCTTTAATACCCCAGCACTCAGCAGGCTGTGACAGCACACCCACCACAAAA
  CCCCAGTGGAGTTTGGAACTTGCACGGAAGGGAACAGGTAAAGAACAAGCACCTTTGGAG
  ATGAGTATGCATCCAGCGGCAAGCGCTCCACTCTCAGTCTTTACTAAGGAATCTACAGCC
  TCCAAACACAGTGACCACCATCACCACCATCACCATGAGCACAAGAAAAAGAAGAAGAAG
  CATAAACATAAGCACAAACACAAGCATAAGCATGACAGTAAAGAAAAGGACAAGGAGCCT
  TTCACTTTCTCCAGCCCTGCCAGTGGCAGGTCTATTCGTTCTCCTTCCCTTTCAGACTGA
  
  Clone variation with respect to NM_003184.3
  2319 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003184 unedited
  NGGTTCACATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACCAGGCGGA
  TGGTAGCTTCGAGCCCTTGCGGAGAGGAGCATCTCTGTGACAGAAGCTTGTCGACGGCGG
  CTTCTAGGAGCTAGTCGAAGGAGCGAGGTTGAGGCGGGCAGCGACCCGTCAGGTCGCTCA
  CCTGGGCACCGGCCAGCTGCGAGACGTGACTTGGGGACCGCAGGGGAGTGGAGAGTGTGA
  GGTGCCAAAGACTAGTAATGCCCCGTATCCCCCTAGGAAGCCGGGAAGCCAAGCTCCGCG
  GGACCGCTTCATGCCGCTGACTGGTGTAGAGCCCGCCAGAATGAACAGGAAGAAAGGAGA
  CAAGGGCTTTGAAAGCCCAAGGCCATATAAATTAACCCATCAGGTCGTCTGCATCAACAA
  CATAAATTTCCAGAGAAAATCTGTTGTGGGATTTGTGGAACTGACTATATTTCCCACAGT
  TGCAAACTTGAATAGAATCAAGTTGAACAGCAAACAGTGTAGAATATACCGAGTAAGGAT
  CAATGATTTAGAGGCTGCTTTTATTTATAATGACCCAACCTTGGAAGTTTGTCACAGTGA
  ATCAAAACAGAGAAACCTCAATTATTTTTCCAATGCTTATGCAGCTGCAGTTAGTGCTGT
  GGACCCTGATGCACGAAATGGAGAACTTTGCATTAAGGTTCCATCAGAGCTATGGAAACA
  CGTTGATGAGTTAAAGGTCCTGGAGATACACATCAATTTTCCTTTGGGACAGCCCAAAAG
  AGGTCTTTATTTTGTGGTACCCACTGTATAGGGTAGTTTGGCAGAAAAGAGGGCTCATGT
  TTCTCTTGTGGCATTCAAAATTTTACAGATTTGGTTCCCTTGGGGGGAATCATACTCTGC
  AATGGGTACCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003184 unedited
  GGGGACAATGNGTGNACCGCGGACCGTCATTTTAGGANCGGATTTTTTTTTTTTTTTTTT
  TTTAAACTTAAAAGTTATTTATTAAACAAGTTAAACACAACTAAGAATATATTTAGAGGT
  CCAAGATTCAAGTATTTTTGTCAAACTTTCTAATGATAAGGGGAATGATAAAAATTGAAC
  AGATATAAAAAATATTCTTAAACAAATATTAAAGCACATGGTAAAATTCAGAAATAAAAA
  CACACCACCATATAAAGAAATCAAAATATTTCATATGTTTTTAAATGCTTATGGTATGAG
  AGCCAAATTGTCTATTTCCAGGTTAATAAACAATATATAAGCTCACCTTTTTAAAGGTAT
  CATACTTTGTGTCATATAGAAATAATTTTGGAAACAGTATGTGTTGGGTGTGTAAATTGT
  CCACATTAAGCAAAACATATTTTACATATGAATATTTTCATTTATACTTACTGGAAAACA
  AAACAAAAAACTTATAATTTAAACATCTTGATTTGAAAATAATTTTGGATTTGATAGTTC
  TGCTAAATTCCAACATCGTAACCGCCGAATGTGTTATCAAAAGATAGTTATTCAAAATTT
  TTTTAAAAAAGAATTGCTTCCCCAAATCTCAGTATTTTAGAGCAAAAGGAAAAATAACAT
  ATTCTTTGTTCATAAATTCTGATGCTTAGTTTCTACGTTAATACAAAAATGGTGCAATAG
  GGAAGAGTACTGTAGAAAAGCCACTGTCTTTTCCAGTAGTTCCAAAATGGTGTTATACAA
  TATTTCACTGGGAGATAGTGGTTGCACCACAGATTTGCCAGATGCTTTTTGTGTAAATCA
  TAACTTTGCCCCATAACATCCACCAAGGCTTNTCAACATAATTGACTTTGGCGGTACACA
  TGGAAACCATGAGCTACCGGGACTGCCAATGGAATGAGGGCTTTATGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003184
• Insert Size: 10120 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003184.2, NP_003175.1
• RefSeq Size: 5019 bp
• RefSeq ORF: 3600 bp
• Locus ID: 6873
• Cytogenetics: 8q24.12
• Domains: Peptidase_M1
• Protein Families: Protease, Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. [provided by RefSeq, Jul 2008]'