SOAT 1 (SOAT1) (NM_003101) Human Untagged Clone

CAT#: SC118156

SOAT1 (untagged)-Human sterol O-acyltransferase 1 (SOAT1), nuclear gene encoding mitochondrial protein, transcript variant 688113

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SOAT1
• Synonyms: ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118156 sequence for NM_003101 edited (data generated by NextGen Sequencing)
  ATGGTGGGTGAAGAGAAGATGTCTCTAAGAAACCGGCTGTCAAAGTCCAGGGAAAATCCT
  GAGGAAGATGAAGACCAGAGAAACCCTGCAAAGGAGTCCCTAGAGACACCTAGTAATGGT
  CGAATTGACATAAAACAGTTGATAGCAAAGAAGATAAAGTTGACAGCAGAGGCAGAGGAA
  TTGAAGCCATTTTTTATGAAGGAAGTTGGCAGTCACTTTGATGATTTTGTGACCAATCTC
  ATTGAAAAGTCAGCATCATTAGATAATGGTGGGTGCGCTCTCACAACCTTTTCTGTTCTT
  GAAGGAGAGAAAAACAACCATAGAGCGAAGGATTTGAGAGCACCTCCAGAACAAGGAAAG
  ATTTTTATTGCAAGGCGCTCTCTCTTAGATGAACTGCTTGAAGTGGACCACATCAGAACA
  ACATATCACATGTTTATTGCCCTCCTCATTCTCTTTATCCTCAGCACACTTGTAGTAGAT
  TACATTGATGAAGGAAGGCTGGTGCTTGAGTTCAGCCTCCTGTCTTATGCTTTTGGCAAA
  TTTCCTACCGTTGTTTGGACCTGGTGGATCATGTTCCTGTCTACATTTTCAGTTCCCTAT
  TTTCTGTTTCAACATTGGGCCACTGGCTATAGCAAGAGTTCTCATCCGCTGATCCGTTCT
  CTCTTCCATGGCTTTCTTTTCATGATCTTCCAGATTGGAGTTCTAGGTTTTGGACCAACA
  TATGTTGTGTTAGCATATACACTGCCACCAGCTTCCCGGTTCATCATTATATTCGAGCAG
  ATTCGTTTTGTAATGAAGGCCCACTCATTTGTCAGAGAGAACGTGCCTCGGGTACTAAAT
  TCAGCTAAGGAGAAATCAAGCACTGTTCCAATACCTACAGTCAACCAGTATTTGTACTTC
  TTATTTGCTCCTACCCTTATCTACCGTGACAGCTATCCCAGGAATCCCACTGTAAGATGG
  GGTTATGTTGCTATGAAGTTTGCACAGGTCTTTGGTTGCTTTTTCTATGTGTACTACATC
  TTTGAAAGGCTTTGTGCCCCCTTGTTTCGGAATATCAAACAGGAGCCCTTCAGCGCTCGT
  GTTCTGGTCCTATGTGTATTTAACTCCATCTTGCCAGGTGTGCTGATTCTCTTCCTTACT
  TTTTTTGCCTTTTTGCACTGCTGGCTCAATGCCTTTGCTGAGATGTTACGCTTTGGTGAC
  AGGATGTTCTATAAGGATTGGTGGAACTCCACGTCATACTCCAACTATTATAGAACCTGG
  AATGTGGTGGTCCATGACTGGCTATATTACTATGCTTACAAGGACTTTCTCTGGTTTTTC
  TCCAAGAGATTCAAATCTGCTGCCATGTTAGCTGTCTTTGCTGTATCTGCTGTAGTACAC
  GAATATGCCTTGGCTGTTTGCTTGAGCTTTTTCTATCCCGTGCTGTTCGTGCTCTTCATG
  TTCTTTGGAATGGCTTTCAACTTCATTGTCAATGATAGTCGGAAAAAGCCGATTTGGAAT
  GTTCTGATGTGGACTTCTCTTTTCTTGGGCAATGGAGTCTTACTCTGCTTTTATTCTCAA
  GAATGGTATGCACGTCGGCACTGTCCTCTGAAAAATCCCACATTTTTGGATTATGTCCGG
  CCACGTTCCTGGACTTGTCGTTACGTGTTTTAG
  
  Clone variation with respect to NM_003101.4
  422 t=>c;969 c=>t;1425 c=>g;1577 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003101 unedited
  TTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCCTGGGCGCCAGGAGAGC
  TTCCCGGAGTCGACCTTCCTGCTGGCTGCTCTGTGACCGCTTCCCGGCTCTGCCCTCTTG
  GCCGAAGTGCCCGCTGCCGGGCGCGGGCCTCAGACAATACAATGGTGGGTGAAGAGAAGA
  TGTCTCTAAGAAACCGGCTGTCAAAGTCCAGGGAAAATCCTGAGGAAGATGAAGACCAGA
  GAAACCCTGCAAAGGAGTCCCTAGAGACACCTAGTAATGGTCGAATTGACATAAAACAGT
  TGATAGCAAAGAAGATAAAGTTGACAGCAGAGGCAGAGGAATTGAAGCCATTTTTTATGA
  AGGAAGTTGGCAGTCACTTTGATGATTTTGTGACCAATCTCATTGAAAAGTCAGCATCAT
  TAGATAATGGTGGGTGCGCTCTCACAACCTTTTCTGTTCTTGAAGGAGAGAAAAACAACC
  ATAGAGCGAAGGATTTGAGAGCACCTCCAGAACAAGGAAAGATTTTTATTGCAAGGCGCT
  CTCTCTTAGATGAACTGCTTGAAGTGGACCACATCAGAACAACATATCACATGTTTATTG
  CCCTCCTCATTCTCTTTATCCTCAGCACACTTGTAGTAGATTACATTGATGAAGGAAGGC
  TGGTGCTTGAGTTCAGCCTCCTGTCTTATGCTTTTGGCAAATTTCCTACCGTTGTTTGGA
  CCTGGTGGATCATGTTCCTGTCTAATTTTCAGTTCCCTATTTTCTGTTTCACATTGGGCC
  ACTGGCTATAGNNCAGAGTCTCATCCGCTGATCCGTTCTCTCTTCATGGCTNTCTTTTCA
  TGATCTTCCANATGGNAGTTCTAGGTTNTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003101 unedited
  AGCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTCCTTCCCAAAAAAGTCATATTTATAGTATTTCTTAACAGACATAT
  AAACCTAAAAATTCCATTCTCTAAATTCAAAAAATTGGCTTGCTCATAACCAGGTCTCAG
  CGCAATCTGTCCCCAATATATTTAAAATGCAGTTATAAATTCAAAATAAAAATTCGTTTT
  AATTTTAAAAAAAAATGTCAAGAATTACAAGCCATATTAAAGCAAACTAGTTAGCAGGTT
  ATGATTCCATTACAATTACAATACTGTTGTGCAAAACATTACTGCGGGAACATGCAAAGT
  ACCTTTTTAAAAATAACTTCCCTTGAGTGTATTTGTTTCCGTTGAAGTTGGATTTAATGT
  TTGAAAATTACCTAAACTCATCTGGACCAGTGGTATCGTGAGTAAAATACGTGATTAAGC
  TGGATACTTGGGGTTCAAATTAAGTTGGGACTATTAAAAAACTCTACTTACCTTCTTGTG
  AGACTCAAACTGACTTTAAAACAATTCCAAACAATACCAAGAGAGCCTCTCAAGTGGCTG
  TTCCAAGTAACAACTATCATTTAAATAATTCATAAATCTTACTTTGAGTGGAAAGTTGAA
  CTACATGGTCTCTGATACCCCTTCCATGCTCAATCTTTACAATCATACCTCATACATACA
  AATAATTTCTACACACTTGTATTAGAAATGGGCCTCTTATAAAATGGAAGTTTTACCTAA
  TAGAGTACATTTCCCTTGAACCCAATCCATATTAATTTTTCCCCCCTAAAACATCCACCC
  TTAAAAGTTAACTGGACAATGCCGAACCCCGCTTCTGGGATCTTAGGATCCCTTAATATG
  AGGGGAGGGATTAAACTTAGGTACGAACTCCCATCTGCAAAAAATTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003101
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003101.4, NP_003092.4
• RefSeq Size: 3407 bp
• RefSeq ORF: 1653 bp
• Locus ID: 6646
• Cytogenetics: 1q25.2
• Domains: MBOAT
• Protein Families: Transmembrane
• Protein Pathways: Steroid biosynthesis
• Gene Summary: 'The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.