RSK1 p90 (RPS6KA1) (NM_002953) Human Untagged Clone

CAT#: SC118295

RPS6KA1 (untagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS6KA1
• Synonyms: HU-1; MAPKAPK1A; p90Rsk; RSK; RSK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002953 edited
  ATGCCGCTCGCCCAGCTCAAGGAGCCCTGGCCGCTCATGGAGCTAGTGCCGCTGGACCCG
  GAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGATGAGGGCGTC
  CTCAAGGAGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCC
  CATTTCGAGCTCCTCAAGGTTCTGGGCCAGGGATCCTTTGGCAAAGTCTTCCTGGTGCGG
  AAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGAAGGTGCTGAAGAAGGCAACG
  CTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAAAT
  CACCCATTCGTGGTGAAGCTGCACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATT
  CTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGATGTTCACG
  GAGGAGGATGTGAAGTTTTACCTGGCCGAGCTGGCTCTGGGCCTGGATCACCTGCACAGC
  CTGGGTATCATTTACAGAGACCTCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCAC
  ATCAAACTCACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCACGAGAAGAAGGCCTAT
  TCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACTCC
  CATAGTGCGGACTGGTGGTCCTATGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTG
  CCCTTCCAGGGGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGAAGCTAGGC
  ATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGAAT
  CCTGCCAACCGGCTCGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTC
  TACTCCACCATTGACTGGAATAAGCTATACCGTCGTGAGATCAAGCCACCCTTCAAGCCA
  GCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCGAGTTCACGTCCCGCACACCC
  AAGGATTCCCCAGGCATCCCCCCCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGC
  TTCGTGGCCACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCAGGCACCCCTG
  CACTCGGTGGTACAGCAACTCCATGGGAAGAACCTGGTTTTTAGTGACGGCTACGTGGTA
  AAGGAGACAATTGGTGTGGGCTCCTACTCTGAGTGCAAGCGCTGTGTCCACAAGGCCACC
  AACATGGAGTATGCTGTCAAGGTCATTGATAAGAGCAAGCGGGATCCTTCAGAAGAGATT
  GAGATTCTTCTGCGGTATGGCCAGCACCCCAACATCATCACTCTGAAAGATGTGTATGAT
  GATGGCAAACACGTGTACCTGGTGACAGAGCTGATGCGGGGTGGGGAGCTGCTGGACAAG
  ATCCTGCGGCAGAAGTTCTTCTCAGAGCGGGAGGCCAGCTTTGTCCTGCACACCATTGGC
  AAAACTGTGGAGTATCTGCACTCACAGGGGGTTGTGCACAGGGACCTGAAGCCCAGCAAC
  ATCCTGTATGTGGACGAGTCCGGGAATCCCGAGTGCCTGCGCATCTGTGACTTTGGTTTT
  GCCAAACAGCTGCGGGCTGAGAATGGGCTCCTCATGACACCTTGCTACACAGCCAACTTT
  GTGGCGCCTGAGGTGCTGAAGCGCCAGGGCTACGATGAAGGCTGCGACATCTGGAGCCTG
  GGCATTCTGCTGTACACCATGCTGGCAGGATATACTCCATTTGCCAACGGTCCCAGTGAC
  ACACCAGAGGAAATCCTAACCCGGATCGGCAGTGGGAAGTTTACCCTCAGTGGGGGAAAT
  TGGAACACAGTTTCAGAGACAGCCAAGGACCTGGTGTCCAAGATGCTACACGTGGATCCC
  CACCAGCGCCTCACAGCTAAGCAGGTTCTGCAGCATCCATGGGTCACCCAGAAAGACAAG
  CTTCCCCAAAGCCAGCTGTCCCACCAGGACCTACAGCTTGTGAAGGGAGCCATGGCTGCC
  ACGTACTCCGCACTCAACAGCTCCAAGCCCACCCCCCAGCTGAAGCCCATCGAGTCATCC
  ATCCTGGCCCAGCGGCGAGTGAGGAAGTTGCCATCCACCACCCTGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002953 unedited
  GGGGTGCATATATTTGTATACGACTCACTATAGGGCGGCCGCGCCGAATGCTAGTGCCGC
  GGCGGCGGCGGCGGACGGCCCAGCCGGAGCGCGAGGGGCTCGGGGGGGCGCGGCGGTTCG
  GGTCGCAGAGCCAGGGACCCCAGGACCCGGGAGGCGGCGCAGCCGGGGCCGCCGGAGGAG
  CGCGGGTGACCTGGCGGCGGCGAGATGCCGCTCGCCCAGCTCAAGGAGCCCTGGCCGCTC
  ATGGAGCTAGTGCCGCTGGACCCGGAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTT
  CAGCCGTCCAAGGATGAGGGCGTCCTCAAGGAGATCTCCATCACGCACCACGTCAAGGCT
  GGCTCTGAGAAGGCTGATCCATCCCATTTCGAGCTCCTCAAGGTTCTGGGCCAGGGATCC
  TTTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCT
  ATGAAGGTGCTGAAGAAGGCAACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAG
  AGAGACATCCTGGCTGATGTAAATCACCCATTCGTGGTGAAGCTGCACTATGCCTTCCAG
  ACCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGG
  CTCTCAAAAGAGGTGATGTTCACGGAGGAGGATGTGAAGTTTTACCTGGCCGAGCTGGCT
  CTGGGCCTGGATCACCTGCACAGCCTGGGTATCATTTACAGAGACCTCAAGCCCTGAGAC
  ATCCTTCTGGATTGAGGAGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAAGAGCCA
  TTGACCACGAGAGAGGCCTATTCTTTCTGCGGGACAGTGAAGTACATGCCCCCTGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002953 unedited
  ACCGCGGGCCGCAATTTAGNATCGAGTTTTTTTTTTTTTTTTTTAAACTCAACAGTTTAT
  TAAAAAGGCCATGGACAGATGAACCCTGAGTAACCAGCTGAGGAAAAAATGAAAAAAGAC
  CCTGTCCCTCATGGCCCGCCCACTGGCCTCCTGTGAACTCTGTCCTGTTGCCAACCCCAG
  ATGAAGTCAGCCAAAAAGTGCTTTCCACATCCTTTCTCTGGGGCTGCCCAGCCTGACCGC
  AGGGGATCCACTGGCAAAGCCAAGGTGGATGCTGGTGCCTGAAGCTGGAAGCCAGCAGGA
  CATGAGACCCCTCCTGTAGCAGGAAGTGGTTCTAGAACTCCCAGCAGAACAGAACGGAAA
  AGGAGCTGACTGGGGATAGAATGAGTTCTGCTAAACAGCCAAATGCTTTGAGAGAGGTGA
  CACTGGACTGTCTCGGAGGTGTGTGCAAATGGCTACAGGTGGCCAATCATGGGGGTCCCC
  AGGGTGGGATCCCAAAGCTGCTCTAAAGAGTCTCAAAGAGCCCCAGCGTGACTCAGTTCC
  CCTCCCTGGGCCGAAATGGCTGTTTCTGCTCCCAGACGGCAAATGGATCTTGTAAAAATC
  CATGGTGGTTGATGCCATTTTTTTCATAAAAAATAGTTATACAGTGAATCGCTTTCACCA
  GCAGAGCCTGAGCCGATAAGAGTCCATCCTGGGCGAGGGAAAAGGCACTTCTCACCCCCC
  TCATTAGGGGTGTTTCTGGGGTTGGCAGCCTTCCCGGCCCCCTCGGTTACAGCTCCCTCT
  GGCCTTGTGTTCCCGGCTGCTCCCTTTGGAAGCATGTTGACTTTGTAAGCTAACACCCCC
  GTGTGGCCCAAAGTCTTGGGGCCCCACAGGCGGCGGAAGGAAACTTCCTACTCGCCGCTG
  GGCCAGAAGGATGACTCGATGGCTTCAATCGAGGGGCGGCCTTGAACTTCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002953
• Insert Size: 3670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002953.3, NP_002944.2
• RefSeq Size: 3199 bp
• RefSeq ORF: 2208 bp
• Locus ID: 6195
• Cytogenetics: 1p36.11
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: 'This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a.