RAF1 (NM_002880) Human Untagged Clone

CAT#: SC118322

RAF1 (untagged)-Human v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1)


  "NM_002880" in other vectors (9)

Reconstitution Protocol

SC118322 is the updated version of SC118323.

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RAF1
Synonyms c-Raf; CMD1NN; CRAF; NS5; Raf-1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_002880 edited
ATGGAGCACATACAGGGAGCTTGGAAGACGATCAGCAATGGTTTTGGATTCAAAGATGCC
GTGTTTGATGGCTCCAGCTGCATCTCTCCTACAATAGTTCAGCAGTTTGGCTATCAGCGC
CGGGCATCAGATGATGGCAAACTCACAGATCCTTCTAAGACAAGCAACACTATCCGTGTT
TTCTTGCCGAACAAGCAAAGAACAGTGGTCAATGTGCGAAATGGAATGAGCTTGCATGAC
TGCCTTATGAAAGCACTCAAGGTGAGGGGCCTGCAACCAGAGTGCTGTGCAGTGTTCAGA
CTTCTCCACGAACACAAAGGTAAAAAAGCACGCTTAGATTGGAATACTGATGCTGCGTCT
TTGATTGGAGAAGAACTTCAAGTAGATTTCCTGGATCATGTTCCCCTCACAACACACAAC
TTTGCTCGGAAGACGTTCCTGAAGCTTGCCTTCTGTGACATCTGTCAGAAATTCCTGCTC
AATGGATTTCGATGTCAGACTTGTGGCTACAAATTTCATGAGCACTGTAGCACCAAAGTA
CCTACTATGTGTGTGGACTGGAGTAACATCAGACAACTCTTATTGTTTCCAAATTCCACT
ATTGGTGATAGTGGAGTCCCAGCACTACCTTCTTTGACTATGCGTCGTATGCGAGAGTCT
GTTTCCAGGATGCCTGTTAGTTCTCAGCACAGATATTCTACACCTCACGCCTTCACCTTT
AACACCTCCAGTCCCTCATCTGAAGGTTCCCTCTCCCAGAGGCAGAGGTCGACATCCACA
CCTAATGTCCACATGGTCAGCACCACCCTGCCTGTGGACAGCAGGATGATTGAGGATGCA
ATTCGAAGTCACAGCGAATCAGCCTCACCTTCAGCCCTGTCCAGTAGCCCCAACAATCTG
AGCCCAACAGGCTGGTCACAGCCGAAAACCCCCGTGCCAGCACAAAGAGAGCGGGCACCA
GTATCTGGGACCCAGGAGAAAAACAAAATTAGGCCTCGTGGACAGAGAGATTCAAGCTAT
TATTGGGAAATAGAAGCCAGTGAAGTGATGCTGTCCACTCGGATTGGGTCAGGCTCTTTT
GGAACTGTTTATAAGGGTAAATGGCACGGAGATGTTGCAGTAAAGATCCTAAAGGTTGTC
GACCCAACCCCAGAGCAATTCCAGGCCTTCAGGAATGAGGTGGCTGTTCTGCGCAAAACA
CGGCATGTGAACATTCTGCTTTTCATGGGGTACATGACAAAGGACAACCTGGCAATTGTG
ACCCAGTGGTGCGAGGGCAGCAGCCTCTACAAACACCTGCATGTCCAGGAGACCAAGTTT
CAGATGTTCCAGCTAATTGACATTGCCCGGCAGACGGCTCAGGGAATGGACTATTTGCAT
GCAAAGAACATCATCCATAGAGACATGAAATCCAACAATATATTTCTCCATGAAGGCTTA
ACAGTGAAAATTGGAGATTTTGGTTTGGCAACAGTAAAGTCACGCTGGAGTGGTTCTCAG
CAGGTTGAACAACCTACTGGCTCTGTCCTCTGGATGGCCCCAGAGGTGATCCGAATGCAG
GATAACAACCCATTCAGTTTCCAGTCGGATGTCTACTCCTATGGCATCGTATTGTATGAA
CTGATGACGGGGGAGCTTCCTTATTCTCACATCAACAACCGAGATCAGATCATCTTCATG
GTGGGCCGAGGATATGCCTCCCCAGATCTTAGTAAGCTATATAAGAACTGCCCCAAAGCA
ATGAAGAGGCTGGTAGCTGACTGTGTGAAGAAAGTAAAGGAAGAGAGGCCTCTTTTTCCC
CAGATCCTGTCTTCCATTGAGCTGCTCCAACACTCTCTACCGAAGATCAACCGGAGCGCT
TCCGAGCCATCCTTGCATCGGGCAGCCCACACTGAGGATATCAATGCTTGCACGCTGACC
ACGTCCCCGAGGCTGCCTGTCTTCTAG
>OriGene 5' read for NM_002880 unedited
TTGGTTTTGTATCCGACTTCTATAGGCGGCCGCGAATTCGGCACGAGCCGATGTGACCGC
CTCCCGCTCCCTCACCCGCCGCGGGGAGGAGGAGCGGGCGAGAAGCTGCCGCCGAACGAC
AGGACGTTGGGGCGGCCTGGCTCCCTCAGGTTTAAGAATTGTTTAAGCTGCATCAATGGA
GCACATACAGGGAGCTTGGAAGACGATCAGCAATGGTTTTGGATTCAAAGATGCCGTGTT
TGATGGCTCCAGCTGCATCTCTCCTACAATAGTTCAGCAGTTTGGCTATCAGCGCCGGGC
ATCAGATGATGGCAAACTCACAGATCCTTCTAAGACAAGCAACACTATCCGTGTTTTCTT
GCCGAACAAGCAAAGAACAGTGGTCAATGTGCGAAATGGAATGAGCTTGCATGACTGCCT
TATGAAAGCACTCAAGGTGAGGGGCCTGCAACCAGAGTGCTGTGCAGTGTTCAGACTTCT
CCACGAACACAAAGGTAAAAAAGCACGCTTAGATTAGGAATACTAGATGCTGCGTCTTTG
ATTGGNNAGAAGAACTTCAAGTAGATTTCCTGGATCATGTTCCCCTCACAACACACAACT
TTGCTCGGAAGACGTTCCTGAAGCTTGCCTTCTGTGACATCTGTCAGAAATTCCTGCTAA
TGGATTTTCGATGTCAGACTTGTGGCTACAAATTTCATGAGCACTGTAGCACCAAAGTAC
CTACTATGTGTGGTAGACCTTGGAGTAACCATCAGACAACTCTTATTGTTTCCAAATTCC
ACTATTGGTGATAGTGGGAGTCCCAGCCCTACCTTCTTAGACTTTGCGTCGTTTGCGAGA
GTCTGTTTCCAGATGCCTGTTAGTTTTCAGACAGATTTTCTACCCTCACGCCTTCACCTT
AACACCTCCGTCCCTCTTTGAAGGTCCTCTCCAGAGGCAGAGGTCGATTCCCT
>OriGene 3' read for NM_002880 unedited
TGAGGAAGTGGGTCACAGTGGCATGCCCACTCCGGTGTATCTGTATCAGGAAAACAGTCT
ATGCACTCGCGCGCCCCGCAATACTAGTAGTACGAGCTTTTTCTTTTTTTTCTTTTTAAA
TTTAATTTATTTTATTAAAATAACATATTTGAGGGACCATCATATAACTGTTTTTTGTCA
GGTGCAATAAAAACAAAATTAATTTTTTTTTCATCAAGAAAACCTGTTTTCCTGGCTTCC
TTGTATACACATGATGTGACTAAGAAAAACAAGGCTGTTTGTTTGTTTGTTTGATAAAAA
AACAAGGCTGGCCCTGCGGCCCCGCCCCATAGGGGCAGCTCCTGGAAGACAAAATTCAGC
ATGATGGAAGACTGCTCCCTGAGAGGGCTGATATGCGGATTGGCCGAGTGCCTTGCCTGG
AAAACCATCCCAATGCACTGGACACCTTATAATCTGTGAAAGGAGGACGTGTCCCCTAAG
AAAAGTTCCATAGTACCAAAGCAGGCTCCTTCGGGCGGCCAGAGTCTCGGCAGTCCTGGG
CTGTTTGGTGCCTTATGTGCAAAATGTCTGGCGCTGCACCACTCTCTGAAGAAAGTCCCG
CCTGTGACATGCATTCCTCCAGAAGCTGATTTCCAAAATCCCATGTGTCTCCACATCATG
GCTGGACTGCCTGCTACCTTACTTCCTCTAAATACTCATGTACCCAACAGCTGGGGCTGG
GCCCCTGCTTTTTGTCTACCATCAACATCCACTTGCGCATCTACAGAAAGCTGGGCCCTT
GAGCATGGGGTATGTGGGGAGGGAGCAAGACACACCAGCACTGGATATGGCTTCCCTTTT
CCAGGGCCCATGGGNTTGAAAAAAAGGCAATATTATTTTATGCCA
Restriction Sites NotI-NotI     
ACCN NM_002880
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002880.2, NP_002871.1
RefSeq Size 3245 bp
RefSeq ORF 1947 bp
Locus ID 5894
Cytogenetics 3p25.2
Domains pkinase, TyrKc, DAG_PE-bind, S_TKc, RBD
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway
Gene Summary 'This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2), as well as variant 3, encodes isoform b.

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