CtIP (RBBP8) (NM_002894) Human Untagged Clone

CAT#: SC118335

RBBP8 (untagged)-Human retinoblastoma binding protein 8 (RBBP8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RBBP8
• Synonyms: COM1; CTIP; JWDS; RIM; SAE2; SCKL2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118335 sequence for NM_002894 edited (data generated by NextGen Sequencing)
  ATGAACATCTCGGGAAGCAGCTGTGGAAGCCCTAACTCTGCAGATACATCTAGTGACTTT
  AAGGACCTTTGGACAAAACTAAAAGAATGTCATGATAGAGAAGTACAAGGTTTACAAGTA
  AAAGTAACCAAGCTAAAACAGGAACGAATCTTAGATGCACAAAGACTAGAAGAATTCTTC
  ACCAAAAATCAACAGCTGAGGGAACAGCAGAAAGTCCTTCATGAAACCATTAAAGTTTTA
  GAAGATCGGTTAAGAGCAGGCTTATGTGATCGCTGTGCAGTAACTGAAGAACATATGCGG
  AAAAAACAGCAAGAGTTTGAAAATATCCGGCAGCAGAATCTTAAACTTATTACAGAACTT
  ATGAATGAAAGGAATACTCTACAGGAAGAAAATAAAAAGCTTTCTGAACAACTCCAGCAG
  AAAATTGAGAATGATCAACAGCATCAAGCAGCTGAGCTTGAATGTGAGGAAGACGTTATT
  CCAGATTCACCGATAACAGCCTTCTCATTTTCTGGCGTTAACCGGCTACGAAGAAAGGAG
  AACCCCCATGTCCGATACATAGAACAAACACATACTAAATTGGAGCACTCTGTGTGTGCA
  AATGAAATGAGAAAAGTTTCCAAGTCTTCAACTCATCCACAACATAATCCTAATGAAAAT
  GAAATTCTAGTAGCTGACACTTATGACCAAAGTCAATCTCCAATGGCCAAAGCACATGGA
  ACAAGCAGCTATACCCCTGATAAGTCATCTTTTAATTTAGCTACAGTTGTTGCTGAAACA
  CTTGGACTTGGTGTTCAAGAAGAATCTGAAACTCAAGGTCCCATGAGCCCCCTTGGTGAT
  GAGCTCTACCACTGTCTGGAAGGAAATCACAAGAAACAGCCTTTTGAGGAATCTACAAGA
  AATACTGAAGATAGTTTAAGATTTTCAGATTCTACTTCAAAGACTCCTCCTCAAGAAGAA
  TTACCTACTCGAGTGTCATCTCCTGTATTTGGAGCTACCTCTAGTATCAAAAGTGGTTTA
  GATTTGAATACAAGTTTGTCCCCTTCTCTTTTACAGCCTGGGAAAAAAAAACATCTGAAA
  ACACTCCCTTTTAGCAACACTTGTATATCTAGATTAGAAAAAACTAGATCAAAATCTGAA
  GATAGTGCCCTTTTCACACATCACAGTCTTGGGTCTGAAGTGAACAAGATCATTATCCAG
  TCATCTAATAAACAGATACTTATAAATAAAAATATAAGTGAATCCCTAGGTGAACAGAAT
  AGGACTGAGTACGGTAAAGATTCTAACACTGATAAACATTTGGAGCCCCTGAAATCATTG
  GGAGGCCGAACATCCAAAAGGAAGAAAACTGAGGAAGAAAGTGAACATGAAGTAAGCTGC
  CCCCAAGCTTCTTTTGATAAAGAAAATGCTTTCCCTTTTCCAATGGATAATCAGTTTTCC
  ATGAATGGAGACTGTGTGATGGATAAACCTCTGGATCTGTCTGATCGATTTTCAGCTATT
  CAGCGTCAAGAGAAAAGCCAAGGAAGTGAGACTTCTAAAAACAAATTTAGGCAAGTGACT
  CTTTATGAGGCTTTGAAGACCATTCCAAAGGGCTTTTCCTCAAGCCGTAAGGCCTCAGAT
  GGCAACTGCACGTTGCCCAAAGATTCCCCAGGGGAGCCCTGTTCACAGGAATGCATCATC
  CTTCAGCCCTTGAATAAATGCTCTCCAGACAATAAACCATCATTACAAATAAAAGAAGAA
  AATGCTGTCTTTAAAATTCCTCTACATCCACGTGAAAGTTTGGAGACTGAGAATGTTTTA
  GATGACATAAAGAGTGCTGGTTCTCATGAGCCAATAAAAATACAAACCAGGTCAGACCAT
  GGAGGATGTGAACTTGCATCAGTTCTTCAGTTAAATCCATGTAGAACTGGTAAAATAAAG
  TCTCTACAAAACAACCAAGATGTATCCTTTGAAAATATCCAGTGGAGTATAGATCCGGGA
  GCAGACCTTTCTCAGTATAAAATGGATGTTACTGTAATAGATACAAAGGATGGCAGTCAG
  TCAAAATTAGGAGGAGAGACAGTGGACATGGACTGTACATTGGTTAGTGAAACCGTTCTC
  TTAAAAATGAAGAAGCAAGAGCAGAAGGGAGAAAAAAGTTCAAATGAAGAAAGAAAAATG
  AATGATAGCTTGGAAGATATGTTTGATCGGACAACACATGAAGAGTATGAATCCTGTTTG
  GCAGACAGTTTCTCCCAAGCAGCAGATGAAGAGGAGGAATTGTCTACTGCCACAAAGAAA
  CTACACACTCATGGTGATAAACAAGACAAAGTCAAGCAGAAAGCGTTTGTGGAGCCGTAT
  TTTAAAGGTGATGAAAGAGAGACTAGCTTGCAAAATTTTCCTCATATTGAGGTGGTTCGG
  AAAAAAGAGGAGAGAAGAAAACTGCTTGGGCACACGTGTAAGGAATGTGAAATTTATTAT
  GCAGATATGCCAGCAGAAGAAAGAGAAAAGAAATTGGCTTCCTGCTCAAGACACCGATTC
  CGCTACATTCCACCCAACACACCAGAGAATTTTTGGGAAGTTGGTTTTCCTTCCACTCAG
  ACTTGTATGGAAAGAGGTTATATTAAGGAAGATCTTGATCCTTGTCCTCGTCCAAAAAGA
  CGTCAGCCTTACAACGCAATATTTTCTCCAAAAGGCAAGGAGCAGAAGACATAG
  
  Clone variation with respect to NM_002894.2
  1766 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002894 unedited
  GGATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCGGAGGGGT
  CGGCTTTCCCACCGAGGATTTGGCACTCTGGTGAGGGAAAAGGCGAAAGAGAAAAGCGAG
  CAGCCGTCCTTTCACAGCCTCAGAAAGTGCTCGCTTCCCTTCGGGGGCTTTCGCGAATCC
  CGAGGCAATCTCGGAGGCGGTATTTGACCTGTCCAAAGACGACTTGATACCTCTATAATG
  TAACAGAAAAGGTCAGAAAATATTAAGCAAGTAGAAGTGTGGAGCATATTAAGCAAGATG
  AACATCTCGGGAAGCAGCTGTGGAAGCCCTAACTCTGCAGATACATCTAGTGACTTTAAG
  GACCTTTGGACAAAACTAAAAGAATGTCATGATAGAGAAGTACAAGGTTTACAAGTAAAA
  GTAACCAAGCTAAAACAGGAACGAATCTTAGATGCACAAAGACTAGAAGAATTCTTCACC
  AAAAATCAACAGCTGAGGGAACAGCAGAAAGTCCTTCATGAAACCATTAAAGTTTTAGAA
  GATCGGTTAAGAGCAGGCTTATGTGATCGCTGTGCAGTAACTGAAGAACATATGCGGAAA
  AAACAGCAAGAGTTTGAAAATATCCGGCAGCAGAATCTTAAACTTATTACAGAACTTATG
  AATGAAAGGAATACTCTACAGGAAGAAAATAAAAAGCTTTCTGAACAACTCCAGCAGAAA
  ATTGAGAATGATCAACAGCATCAAGCAGCTGAGCTTGAATGGTGAGAAAGACGTATTCCA
  GATTACCGATNACAGCCTTNCTCATTTCTGGCGTTAACCGGCTACGAAGAAGGAGAAACC
  CCCTGTCCGAACATAGAACAAACACTACCTAATGGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002894 unedited
  GCGGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTGTATTTTCAAAATTGTATTAG
  GTATCACTACATAAAGTAAAAACTCTTAAGTTAGAGTGCAAAATGAAAGCGCCTTATTGT
  TTTAAAGGTGCAAAAGCAAAATATCACAAACATTTTCAATAGAAAACTGATTTATAAATC
  CATTTACAGAAGATCAAAAAAATCAATGTTTAGTACCAACTTTAACTATAAATAACTAAG
  AAGGAAAAAACTGGCCTTCATCCTTCTGTTTCTGTTTCAACGTCTATGTCTTCTGCTCCT
  TGCCTTTTGGAGAAAATATTGCGTTGTAAGGCTGACGTCTTTTTGGACGAGGACAAGGAT
  CAAGATCTTCCTTAATATAACCTCTTTCCATACAAGTCTGAGTGGAAGGAAAACCAACTT
  CCCAAAAATTCTCTGGTGTGTTGGGTGGAATGTACCGGAATCGGTGTCTTGAGCAAGAAG
  CCAATTTCTTTACTCTTTCCTCCGCTGGCATATCTGCATAATAAAATGCACATTCCTTTC
  ACCTGGGCCACAACAGGTGGTTCCGCCCGCCGTTTTTTCCCACCCCCCCGTCTCCACGGA
  TATCCTCCCCGCGGCCGCGTCCCCATCACCCCCCCACTCCCCCCCCTCTATCTCCCCCTT
  ATACCATTTTTATCCGACCCGTTCCCCTCCCCCCCGCTCTCCGCCCCTTCACAGTCTTTG
  TCGTGTACTCCTCCCTCCCCCGATTCCCCCCCCTTCCGCCCATCCCCTACCTTTCACCTC
  CCTTTTCTTGTTCCCTCTTCGCTTTCACTCATTCCTCGTCTGCCATCGACCCGTCCCCCC
  TCTTTTTCTCTTTTCAATTTCCGACCCTCCTCATACAATTCGCCTATCCTCCTCCGACTT
  CGCCCGCGCCCCGCGCTTTCTTTTCCCGTGTGGTGCGCGCTCCCTCCCTTTGCTCTTGTT
  A
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002894
• Insert Size: 3240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002894.2, NP_002885.1
• RefSeq Size: 3279 bp
• RefSeq ORF: 2694 bp
• Locus ID: 5932
• Cytogenetics: 18q11.2
• Gene Summary: 'The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 both encode isoform a.