GPBB (PYGB) (NM_002862) Human Untagged Clone

CAT#: SC118380

PYGB (untagged)-Human phosphorylase, glycogen, brain (PYGB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PYGB
• Synonyms: GPBB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118380 sequence for NM_002862 edited (data generated by NextGen Sequencing)
  ATGGCGAAGCCGCTGACGGACAGCGAGAAGCGGAAGCAGATCAGCGTGCGCGGCCTGGCG
  GGGCTAGGCGACGTGGCCGAGGTGCGGAAGAGCTTCAACCGGCACTTGCACTTCACGCTG
  GTCAAGGACCGCAATGTGGCCACGCCCCGCGACTACTTCTTCGCGCTGGCGCACACGGTG
  CGCGACCACCTCGTGGGCCGCTGGATCCGCACGCAGCAGCACTACTACGAGCGCGACCCC
  AAGCGCATTTATTATCTTTCCCTGGAATTCTACATGGGTCGCACGCTGCAGAACACGATG
  GTGAACCTGGGCCTTCAGAATGCCTGCGATGAAGCCATCTATCAGTTGGGGTTAGACTTG
  GAGGAACTCGAGGAGATAGAAGAAGATGCTGGCCTTGGGAATGGAGGCCTGGGGAGGCTG
  GCAGCGTGTTTCCTTGACTCAATGGCTACCTTGGGCCTGGCAGCATACGGCTATGGAATC
  CGCTATGAATTTGGGATTTTTAACCAGAAGATTGTCAATGGCTGGCAGGTAGAGGAGGCC
  GATGACTGGCTGCGCTACGGCAACCCCTGGGAGAAAGCGCGGCCTGAGTATATGCTTCCC
  GTGCACTTCTACGGACGCGTGGAGCACACCCCCGACGGCGTGAAGTGGCTGGACACACAG
  GTGGTGCTGGCCATGCCCTACGACACCCCAGTGCCCGGCTACAAGAACAACACCGTCAAC
  ACCATGCGGCTGTGGTCCGCCAAGGCTCCCAACGACTTCAAGCTGCAGGACTTCAACGTG
  GGAGACTACATCGAGGCGGTCCTGGACCGGAACTTGGCTGAGAACATCTCCAGGGTCCTG
  TATCCAAATGATAACTTCTTTGAGGGGAAGGAGCTGCGGCTGAAGCAGGAGTACTTCGTG
  GTGGCCTCCACGCTCCAGGACATCATCCGCCGCTTCAAGTCGTCCAAGTTCGGCTGCCGG
  GACCCTGTGAGAACCTGTTTCGAGACGTTCCCAGACAAGGTGGCCATCCAGCTGAACGAC
  ACCCACCCCGCCCTCTCCATCCCTGAGCTCATGCGGATCCTGGTGGACGTGGAGAAGGTG
  GACTGGGACAAGGCCTGGGAAATCACGAAGAAGACCTGTGCGTACACCAACCACACTGTG
  CTGCCTGAGGCCTTGGAGCGCTGGCCCGTGTCCATGTTTGAGAAGCTGCTGCCGCGGCAC
  CTGGAGATAATCTATGCCATCAACCAGCGGCACCTGGACCACGTGGCCGCGCTGTTTCCC
  GGCGATGTGGACCGCCTGCGCAGGATGTCTGTGATCGAGGAGGGGGACTGCAAGCGGATC
  AACATGGCCCACCTGTGTGTGATTGGGTCCCATGCTGTCAATGGTGTGGCGAGGATCCAC
  TCGGAGATCGTGAAACAGTCGGTCTTTAAGGATTTTTATGAACTGGAGCCAGAGAAGTTC
  CAGAATAAGACCAATGGCATCACCCCCCGCCGGTGGCTGCTGCTGTGCAACCCGGGGCTG
  GCCAATACCATCGTGGAGAAAATTGGGGAGGAGTTCCTGACTGACCTGAGCCAGCTGAAG
  AAGCTGCTGCCGCTGGTCAGTGACGAGGTGTTCATCAGGGACGTGGCCAAGGTCAAACAG
  GAGAACAAGCTCAAGTTCTCGGCCTTCCTGGAGAAGGAGTACAAGGTGAAGATCAACCCC
  TCCTCCATGTTCGACGTGCATGTGAAGAGGATCCACGAGTACAAGCGGCAGCTGCTCAAC
  TGCCTGCACGTCGTCACCCTGTACAATCGAATCAAGAGAGACCCGGCCAAGGCTTTTGTG
  CCCAGGACTGTTATGATTGGGGGCAAGGCAGCGCCCGGTTACCACATGGCCAAGCTGATC
  ATCAAGTTGGTCACCTCCATCGGCGACGTCGTCAATCATGACCCAGTTGTGGGTGACAGG
  TTGAAAGTGATCTTCCTGGAGAACTACCGTGTGTCCTTGGCTGAGAAAGTGATCCCGGCC
  GCTGATCTGTCGCAGCAGATCTCCACTGCAGGCACCGAGGCCTCAGGCACAGGCAACATG
  AAGTTCATGCTCAACGGGGCCCTCACCATCGGCACCATGGACGGCGCCAACGTGGAGATG
  GCCGAGGAGGCCGGGGCCGAGAACCTCTTCATCTTCGGCCTGCGGGTGGAGGATGTCGAG
  GCCTTGGACCGGAAAGGGTACAATGCCAGGGAGTACTACGACCACCTGCCCGAGCTGAAG
  CAGGCCGTGGACCAGATCAGCAGTGGCTTTTTTTCTCCCAAGGAGCCAGACTGCTTCAAG
  GACATCGTGAACATGCTGATGCACCATGACAGGTTCAAGGTGTTTGCAGACTATGAAGCC
  TACATGCAGTGCCAGGCACAGGTGGACCAACTGTACCGGAACCCCAAGGAGTGGACCAAG
  AAGGTCATCAGGAACATCGCCTGCTCGGGCAAGTTCTCCAGTGACCGGACCATCACGGAG
  TATGCACGGGAGATCTGGGGTGTGGAGCCCTCCGACCTGCAGATCCCGCCCCCCAACATC
  CCCCGGGACTAG
  
  Clone variation with respect to NM_002862.3
  907 g=>t;1122 a=>g;1504 g=>a;1695 t=>c;2370 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002862 unedited
  AGATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCGCCAGAGCAGCTG
  CACCATCCCGGCGTTCGCGTGTGCCGCCGCTTTCCTCCTCCATCTCTTTTCCTCCGCCTC
  CGCCGGCGCGATGGCGAAGCCGCTGACGGACAGCGAGAAGCGGAAGCAGATCAGCGTGCG
  CGGCCTGGCGGGGCTAGGCGACGTGGCCGAGGTGCGGAAGAGCTTCAACCGGCACTTGCA
  CTTCACGCTGGTCAAGGACCGCAATGTGGCCACGCCCCGCGACTACTTCTTCGCGCTGGC
  GCACACGGTGCGCGACCACCTCGTGGGCCGCTGGATCCGCACGCAGCAGCACTACTACGA
  GCGCGACCCCAAGCGCATTTATTATCTTTCCCTGGAATTCTACATGGGTCGCACGCTGCA
  GAACACGATGGTGAACCTGGGCCTTCAGAATGCCTGCGATGAAGCCATCTATCAGTTGGG
  GTTAGACTTGGAGGAACTCGAGGAGATAGAAGAAGATGCTGGCCTTGGGAATGGAGGCCT
  GGGGAGGCTGGCAGCGTGTTTCCTTGACTCAATGGCTACCTTGGGCCTGGCAGCATACGG
  CTATGGAATCCGCTATGAATTTGGGATTTTTAACCAGAAGATTGTCAATGGCTGGCAGGT
  AGAGGAGGCCGATGACTGGCTGCGCTACGGCAACCCCTGGGAGAAAGCGCGGCCTGAGTA
  TATGCTTNCCGTGCACTTCTACGGACGCGTGGAGCACACCCNCGACGGCGTGAAGTGGCT
  GGACACACANGTGGTGCTGGCCATGCCCTACGACACCCCAGTGCCCGGGCTACAGAACAC
  ACCGTCAACACATGCGGNTGTGGTCCGNNCAGGCTCCNNACGACTCAGCTGCAGACTTCA
  CGTGGGAACTAATCGAGGCGGTCTGGACGNNACTGCTGANACACTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002862 unedited
  NTTTTTACTCTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTCAACACCCACAAAGGTTAAATAATTTTTTATT
  TAACAAGGTAAAAACAATGAGGGCCACAGGGGGGGTAAGGGGGGGGCCCCCAGGGCCCAA
  TTAGGGGAGTTTGGCCTCTTCCAAAAACCCGTTGCTTGGGAAAAATGTTGTAAGGGCACA
  ACCACAGCCAAAAATTTGCCCCACCCCATTAAAAAAAAGGTTGAAGGGTTTTGGGGGGTT
  GGCCACCGTGCCCAAATCCTCTTGGGGGGCATTGGAGGCCAGGAAAAAAAAAACCCTCCC
  CGAACCCAAAATCCAGATTGCAGGGGGTTAAATTTTTCGGAATTTCCACAGGAAAGGACA
  GAACCCTGTTCCTTGCCTTTTTTTTTGGCCAAGGAAAGGCAGTTTCCCACCCTTGGGAAG
  GGGCAAGGTTGTAAGGGGTTAACCCCCACTTCCAAGTGCCAGGCCTGTTGTCAAAAAAAA
  GAAAAAATGGGTTCCTCCGGAACCCCTGGTCTTTTTTCAAAACAAGGTTGTGGCATTGGT
  GAAAAGGGAGGAAAAATGGACCCTGCCCTTTGTGTGAACAACAGGAAATGCACCTAAGAA
  CACAGCAGGAGTCAGGGGGAGGGCACAAACCTGCCCCCTGCCAGGAAAAAAATGGCCCTC
  CTAAACCACAAAAGTGACCAAGTCCATTTTTCAGTTGTTAAAACAAGAAAAGGCTTCAAC
  TAATTCAATTCCCATGGGNAGTTATTTTTCTCTTTGAAAAAGCAAAACCAAGCTAAGCTG
  NGTTAACCAATTTAATCCTGAACAAGGCCTTTGAAAAAAAAAACCAGCCCCCACTCCAGG
  TCCCCCCTAATCTTCCGGGTTTCCAACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002862
• Insert Size: 4370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002862.3, NP_002853.2
• RefSeq Size: 4131 bp
• RefSeq ORF: 2532 bp
• Locus ID: 5834
• Cytogenetics: 20p11.21
• Domains: phosphorylase
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism
• Gene Summary: 'The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]'