PSMB3 (NM_002795) Human Untagged Clone

CAT#: SC118416

PSMB3 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 3 (PSMB3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMB3
• Synonyms: HC10-II
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118416 sequence for NM_002795 edited (data generated by NextGen Sequencing)
  ATGTCTATTATGTCCTATAACGGAGGGGCCGTCATGGCCATGAAGGGGAAGAACTGTGTG
  GCCATCGCTGCAGACAGGCGCTTCGGGATCCAGGCCCAGTTGGTGACCACGGACTTCCAG
  AAGATCTTTCCCATGGGTGACCGGCTGTACATCGGTCTGGCCGGGCTCGCCACTGACGTC
  CAGACAGTTGCCCAGCGCCTCAAGTTCCGGCTGAACCTGTATGAGTTGAAGGAAGGTCGG
  CAGATCAAACCTTATACCCTCATGAGCATGGTGGCCAACCTCTTGTATGAGAAACGGTTT
  GGCCCTTACTACACTGAGCCAGTCATTGCCGGGTTGGACCCGAAGACCTTTAAGCCCTTC
  ATTTGCTCTCTAGACCTCATCGGCTGCCCCATGGTGACTGATGACTTTGTGGTCAGTGGC
  ACCTGCGCCGAACAAATGTACGGAATGTGTGAGTCCCTCTGGGAGCCCAACATGGATCCG
  GATCACCTGTTTGAAACCATCTCCCAAGCCATGCTGAATGCTGTGGACCGGGATGCAGTG
  TCAGGCATGGGAGTCATTGTCCACATCATCGAGAAGGACAAAATCACCACCAGGACACTG
  AAGGCCCGAATGGACTAA
  
  Clone variation with respect to NM_002795.2
  100 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002795 unedited
  GGTCAGGAATAGTATCCGATTCATATAGGCGGCCGCGCAATTCGCACGAGGGCTAGACCC
  GGTTTACTGGAATTGCTCTGGCGATCGAGGGGTCCTAGTACACCGCAATCATGTCTATTA
  TGTCCTATAACGGAGGGGCCGTCATGGCCATGAAGGGGAAGAACTGTGTGGCCATCGCTG
  CAGACAGGCGCTTCGGGATCCAGGCCCTGTTGGTGACCACGGACTTCCAGAAGATCTTTC
  CCATGGGTGACCGGCTGTACATCGGTCTGGCCGGGCTCGCCACTGACGTCCAGACAGTTG
  CCCAGCGCCTCAAGTTCCGGCTGAACCTGTATGAGTTGAAGGAAGGTCGGCAGATCAAAC
  CTTATACCCTCATGAGCATGGTGGCCAACCTCTTGTATGAGAAACGGTTTGGCCCTTACT
  ACACTGAGCCAGTCATTGCCGGGTTGGACCCGAAGACCTTTAAGCCCTTCATTTGCTCTC
  TAGACCTCATCGGCTGCCCCATGGTGACTGATGACTTTGTGGTCAGTGGCACCTGCGCCG
  AACAAATGTACGGAATGTGTGAGTCCCTCTGGGAGCCCAACATGGATCCGGATCACCTGT
  TTGAAACCATCTCCCAAGCCATGCTGAATGCTGTGGACCGGGATGCAGTGTCAGGCATGG
  GAGTCATTGTCCACATCATCGAGAAAGACAAAATCACCACCAGGACACTGAAGGCCCGAA
  TGGACTAACCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTCTTGACATAAAATAGCCTT
  GTTTTTCAAAAAACAAAAAAAAAACTCGACTTTAGATTGCGGCCGCGGCCATACCTGTTT
  CCTGAACAGAACCCCGGTGGGATCCCTGTGACCCCTCCCATGGCCTTTCCTGCCCCTGGA
  AATTGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002795 unedited
  TAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAAGACAGGCTATTTTATTTCAAAAAAAGAA
  AAAAAAGTGGGCCTCTGGGAACAGGGTTAGTCCATTCGGGCCTTCAGTGTCCTGGTGGTG
  ATTTTGTCCTTCTCGATGATGTGGACAATGACTCCCATGCCTGACACTGCATCCCGGTCC
  ACAGCATTCAGCATGGCTTGGGAGATGGTTTCAAACAGGTGATCCGGATCCATGTTGGGC
  TCCCAGAGGGACTCACACATTCCGTACATTTGTTCGGCGCAGGTGCCACTGACCACAAAG
  TCATCAGTCACCATGGGGCAGCCGATGAGGTCTAGAGAGCAAATGAAGGGCTTAAAGGTC
  TTCGGGTCCAACCCGGCAATGACTGGCTCAGTGTAGTAAGGGCCAAACCGTTTCTCATAC
  AAGAGGTTGGCCACCATGCTCATGAGGGTATAAGGTTTGATCTGCCGACCTTCCTTCAAC
  TCATACAGGTTCAGCCGGAACTTGANGCGCNTGGGCACTGTCTGGACGTCAGTGGCGAAG
  CCCGCCAGACCGATGTACAGCCGGTCACCCCATGGAAAGATCTTCTGGAGTCCGTGGTCC
  CCAACTGGGCCTGAATCCCGAACCCCTGTCTGCAACAAGGCCACCAGTTCTTCCCCTTCA
  TGCCCTGACGGCCCTCCGTTATGGACATAAAAAACTGAATGGGGGGTCCTGACCCCCCAA
  TCCCCAAAAAATTCCCGTAAACGGGTTTACCCCCTTGGCCAATATCGGGCCGCCCTAAGG
  GGGCCCTATCCAAATTTTACGCGGTCAAAACACACCTTGTTTTATTACCCCCCCCCCGAC
  CCCCCCCCCCCTTTTTGTACCCGGGGGGGGTTTTACCCATTTTTAAAAACCCCCCTTTTT
  TGGCCCAAACCCCCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002795
• Insert Size: 780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002795.2, NP_002786.2
• RefSeq Size: 784 bp
• RefSeq ORF: 618 bp
• Locus ID: 5691
• Cytogenetics: 17q12
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: 'The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The 26 S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants [provided by RefSeq, Sep 2013]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the functional protein.