POLD1 (NM_002691) Human Untagged Clone

CAT#: SC118520

POLD1 (untagged)-Human polymerase (DNA directed), delta 1, catalytic subunit 125kDa (POLD1)


  "NM_002691" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol POLD1
Synonyms CDC2; CRCS10; MDPL; POLD
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_002691, the custom clone sequence may differ by one or more nucleotides


ATGGATGGCAAGCGGCGGCCAGGCCCAGGGCCCGGGGTGCCCCCAAAGCGGGCCCGTGGGGGCCTCTGGG
ATGATGATGATGCACCTCGGCCATCCCAATTCGAGGAGGACCTGGCACTGATGGAGGAGATGGAGGCAGA
ACACAGGCTGCAGGAGCAGGAGGAGGAGGAGCTGCAGTCAGTCCTGGAGGGGGTTGCAGACGGGCAGGTC
CCACCATCAGCCATAGATCCTCGCTGGCTTCGGCCCACACCACCAGCGCTGGACCCCCAGACAGAGCCCC
TCATCTTCCAACAGTTGGAGATTGACCATTATGTGGGCCCAGCGCAGCCTGTGCCTGGGGGGCCCCCACC
ATCCCGCGGCTCCGTGCCTGTGCTCCGCGCCTTCGGGGTCACCGATGAGGGGTTCTCTGTCTGCTGCCAC
ATCCACGGCTTCGCTCCCTACTTCTACACCCCAGCGCCCCCTGGTTTCGGGCCCGAGCACATGGGTGACC
TGCAACGGGAGCTGAACTTGGCCATCAGCCGGGACAGTCGCGGGGGGAGGGAGCTGACTGGGCCGGCCGT
GCTGGCTGTGGAACTGTGCTCCCGAGAGAGCATGTTTGGGTACCACGGGCACGGCCCCTCCCCGTTCCTG
CGCATCACCGTGGCGCTGCCGCGCCTCGTGGCCCCGGCCCGCCGTCTCCTGGAACAGGGCATCCGTGTGG
CAGGCCTGGGCACGCCCAGCTTCGCGCCCTACGAGGCCAACGTCGACTTTGAGATCCGGTTCATGGTGGA
CACGGACATCGTCGGCTGCAACTGGCTGGAGCTCCCAGCTGGGAAATACGCCCTGAGGCTGAAGGAGAAG
GCTACGCAGTGCCAGCTGGAGGCGGACGTGCTGTGGTCTGACGTGGTCAGTCACCCACCGGAAGGGCCAT
GGCAGCGCATTGCGCCCTTGCGCGTGCTCAGCTTCGATATCGAGTGCGCCGGCCGCAAAGGCATCTTCCC
TGAGCCTGAGCGGGACCCTGTCATCCAGATCTGCTCGCTGGGCCTGCGCTGGGGGGAGCCGGAGCCCTTC
CTACGCCTGGCGCTCACCCTGCGGCCCTGTGCCCCCATCCTGGGTGCCAAGGTGCAGAGCTACGAGAAGG
AGGAGGACCTGCTGCAGGCCTGGTCCACCTTCATCCGTATCATGGACCCCGACGTGATCACCGGTTACAA
CATCCAGAACTTCGACCTTCCGTACCTCATCTCTCGGGCCCAGACCCTCAAGGTACAAACATTCCCTTTC
CTGGGCCGTGTGGCCGGCCTTTGCTCCAACATCCGGGACTCTTCATTCCAGTCCAAGCAGACGGGCCGGC
GGGACACCAAGGTTGTCAGCATGGTGGGCCGCGTGCAGATGGACATGCTGCAGGTGCTGCTGCGGGAGTA
CAAGCTCCGCTCCTACACGCTCAATGCCGTGAGCTTCCACTTCCTGGGCGAGCAGAAGGAGGACGTGCAG
CACAGCATCATCACCGACCTGCAGAATGGGAACGACCAGACCCGCCGCCGCCTGGCTGTGTACTGCCTGA
AGGATGCCTACCTGCCACTGCGGCTGCTGGAGCGGCTCATGGTGCTGGTGAACGCCGTGGAGATGGCGAG
GGTCACTGGCGTGCCCCTCAGCTACCTGCTCAGTCGTGGCCAGCAGGTCAAGGTCGTATCCCAGCTGTTG
CGGCAGGCCATGCACGAGGGGCTGCTGATGCCCGTGGTGAAGTCAGAGGGCGGCGAGGACTACACGGGAG
CCACTGTCATCGAGCCCCTCAAAGGGTACTACGACGTCCCCATCGCCACCCTGGACTTCTCCTCGCTGTA
CCCGTCCATCATGATGGCCCACAACCTGTGTTACACCACGCTCCTTCGGCCCGGGACTGCACAGAAACTG
GGCCTGACTGAGGATCAGTTCATCAGGACCCCCACCGGGGACGAGTTTGTGAAGACCTCAGTGCGGAAGG
GGCTGCTGCCCCAGATCCTGGAGAACCTGCTCAGTGCCCGGAAGAGGGCCAAGGCCGAGCTGGCCAAGGA
GACAGACCCCCTCCGGCGCCAGGTCCTGGATGGACGGCAGCTGGCGCTGAAGGTGAGCGCCAACTCCGTA
TACGGCTTCACTGGCGCCCAGGTGGGCAAGTTGCCGTGCCTGGAGATCTCACAGAGCGTCACGGGGTTCG
GACGTCAGATGATCGAGAAAACCAAGCAGCTGGTGGAGTCTAAGTACACAGTGGAGAATGGCTACAGCAC
CAGTGCCAAGGTGGTGTATGGTGACACTGACTCCGTCATGTGCCGATTCGGCGTGTCCTCGGTGGCTGAG
GCGATGGCCCTGGGGCGGGAGGCCGCGGACTGGGTGTCAGGTCACTTCCCGTCGCCCATCCGGCTGGAGT
TTGAGAAGGTCTACTTCCCATACCTGCTTATCAGCAAGAAGCGCTACGCGGGCCTGCTCTTCTCCTCCCG
GCCCGACGCCCACGACCGCATGGACTGCAAGGGCCTGGAGGCCGTGCGCAGGGACAACTGCCCCCTCGTG
GCCAACCTGGTCACTGCCTCACTGCGCCGCCTGCTCATCGACCGAGACCCTGAGGGCGCGGTGGCTCACG
CACAGGACGTCATCTCGGACCTGCTGTGCAACCGCATCGATATCTCCCAGCTGGTCATCACCAAGGAGCT
GACCCGCGCGGCCTCCGACTATGCCGGCAAGCAGGCCCACGTGGAGCTGGCCGAGAGGATGAGGAAGCGG
GACCCCGGGAGTGCGCCCAGCCTGGGCGACCGCGTCCCCTACGTGATCATCAGTGCCGCCAAGGGTGTGG
CCGCCTACATGAAGTCGGAGGACCCGCTGTTCGTGCTGGAGCACAGCCTGCCCATTGACACGCAGTACTA
CCTGGAGCAGCAGCTGGCCAAGCCCCTCCTGCGCATCTTCGAGCCCATCCTGGGCGAGGGCCGTGCCGAG
GCTGTGCTACTGCGGGGGGACCACACGCGCTGCAAGACGGTGCTCACGGGCAAGGTGGGCGGCCTCCTGG
CCTTCGCCAAACGCCGCAACTGCTGCATTGGCTGCCGCACAGTGCTCAGCCACCAGGGAGCCGTGTGTGA
GTTCTGCCAGCCCCGGGAGTCTGAGCTGTATCAGAAGGAGGTATCCCATCTGAATGCCCTGGAGGAGCGC
TTCTCGCGCCTCTGGACGCAGTGCCAGCGCTGCCAGGGCAGCCTGCACGAGGACGTCATCTGCACCAGCC
GGGACTGCCCCATCTTCTACATGCGCAAGAAGGTGCGGAAGGACCTGGAAGACCAGGAGCAGCTCCTGCG
GCGCTTCGGACCCCCTGGACCTGAGGCCTGGTGA


>OriGene 5' read for NM_002691 unedited
ATATCCCCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
CGAATTCGGCACGAGGGCGGCGTAGGCTGTGGCGGGAAACGCTGTTTGAAGCGGGATGGA
TGGCAAGCGGCGGCCAGGCCCAGGGCCCGGGGTGCCCCCAAAGCGGGCCCGTGGGGGCCT
CTGGGATGATGATGATGCACCTCGGCCATCCCAATTCGAGGAGGACCTGGCACTGATGGA
GGAGATGGAGGCAGAACACAGGCTGCAGGAGCAGGAGGAGGAGGAGCTGCAGTCAGTCCT
GGAGGGGGTTGCAGACGGGCAGGTCCCACCATCAGCCATAGATCCTCGCTGGCTTCGGCC
CACACCACCAGCGCTGGACCCCCAGACAGAGCCCCTCATCTTCCAACAGTTGGAGATTGA
CCATTATGTGGGCCCAGCGCAGCCTGTGCCTGGGGGGCCCCCACCATCCCGCGGCTCCGT
GCCTGTGCTCCGCGCCTTCGGGGTCACCGATGAGGGGTTCTCTGTCTGCTGCCACATCCA
CGGCTTCGCTCCCTACTTCTACACCCCAGCGCCCCCTGGTTTCGGGCCCGAGCACATGGG
TGACCTGCAACGGGAGCTGAACTTGGCCATCAGCCGGGACAGTCGCGGGGGGAGGGGAGC
TGACTGGGCCGGCCGTGCTGGCTGTGGAACTGTGCTCCCGAGAGAGCATGTTTGGGTACC
ACGGGCACGNGCCCTCCCCGTTCCTGCGCTCANCGTTGGCGCTGCCGCGCCTCGTGGCCC
CGGCCCGCCGTCTCCTGGGACAGGGCATCCGTGTGGCAGGCCTGGGCACGCCCAGCTTCG
CGCCCTACGAGCCNACGTCN
>OriGene 3' read for NM_002691 unedited
CATATTATGTACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTAACAAAAGTCCAAAACTTTATTAATTCTCCCTGGTCCCGCCCCCGCCC
CATGGGATGCTTGCAAGGTCACCAAGCCTCAAGTCCAGGGGGTCCGAAACGCCGCAAGAG
CTGCTCCTGGTCTTCCAGGTCCTTCCGCACCTTTTTGCGCATGTAAAAAATGGGGCAGTC
CCGGCTGGTGCAAATGACGTCCTCGTGCAAGCTGCCCTGGCAGCGCTGGCACTGCGTCCA
AAGGCGCGAGAAGCGCTCCTCCAGGGCATTCAAATGGGATACCTCCTTCTGATACAGCTC
AAACTCCCGGGGCTGGCAGAACTCACACACGGCTCCCTGGTGGCTGAGCACTGTGCGGCA
GCCAATGCAACATTTGCGGCGTTTGGCGAAAGCCAAGAAGCCGCCCACCTTGCCCGTGAA
CACCGTCTTGCAGCGCGTGTGGTCCCCCCGCAGTAGCACAACCTCGGCACGGCCCTCGCC
CAAGATGGGCTCGAATATGCGCAAGAAGGGCTTGGCCAACTGCTGCTCCAAGTAGTACTG
CGTGTCAATGGGCAAGCTGTGCTCCAGCACGAACAGCGGGTCCTCCGACTTTATGTAAGC
GGGCACACCCTTGGCGGGACTGATGATCACCTATGGGACGCGGGTCCCCAAGCTGGGCGC
ACTTCCAGGGTCCCGCTTTCTCATACTCTTGGGCAACTCCACGTGGGCCTTGTTGCCNGC
TTATTCGGAGGGCCCCCGGGTCAACTTCTTGTGGATGACCCACTGGGAGATATCGATTGC
GTTTGCCAACAAGGTCCCAAAAACCTCCCGTGCGTGACCCACCGGCCCCCCAA
Restriction Sites NotI-NotI     
ACCN NM_002691
Insert Size 3600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002691.1, NP_002682.1
RefSeq Size 3443 bp
RefSeq ORF 3324 bp
Locus ID 5424
Cytogenetics 19q13.33
Domains DNA_pol_B_exo, DNA_pol_B, POLBc
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Base excision repair, DNA replication, Homologous recombination, Metabolic pathways, Mismatch repair, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
Gene Summary 'This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]'
Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR and uses an alternate splice site in the 3' coding region, compared to variant 4. The encoded isoform (1) is shorter than isoform 2.

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