PCOLCE (NM_002593) Human Untagged Clone

CAT#: SC118529

PCOLCE (untagged)-Human procollagen C-endopeptidase enhancer (PCOLCE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCOLCE
• Synonyms: PCPE; PCPE-1; PCPE1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118529 sequence for NM_002593 edited (data generated by NextGen Sequencing)
  ATGCTGCCTGCAGCCACAGCCTCCCTCCTGGGGCCCCTCCTCACTGCCTGCGCCCTGCTG
  CCTTTTGCCCAGGGCCAGACCCCCAACTACACCAGACCCGTGTTCCTGTGCGGAGGGGAT
  GTGAAGGGGGAATCAGGTTACGTGGCAAGTGAGGGGTTCCCCAACCTCTACCCCCCTAAT
  AAGGAGTGCATCTGGACCATAACGGTCCCCGAGGGCCAGACTGTGTCCCTCTCATTCCGA
  GTCTTCGACCTGGAGCTGCACCCCGCCTGCCGCTACGATGCTCTGGAGGTCTTCGCTGGG
  TCTGGGACTTCCGGCCAGCGGCTCGGACGCTTTTGTGGGACCTTCCGGCCTGCGCCCCTA
  GTCGCCCCCGGCAACCAGGTGACCCTGAGGATGACGACGGATGAGGGCACAGGAGGACGA
  GGCTTCCTGCTCTGGTACAGCGGGCGGGCCACCTCGGGCACTGAGCACCAATTTTGCGGG
  GGGCGGCTGGAGAAGGCCCAGGGAACCCTGACCACGCCCAACTGGCCCGAGTCCGATTAC
  CCCCCGGGCATCAGCTGTTCCTGGCACATCATCGCGCCCCCGGACCAGGTCATCGCGCTG
  ACCTTCGAGAAGTTTGACCTGGAGCCGGACACCTACTGCCGCTATGACTCGGTCAGCGTG
  TTCAACGGAGCCGTGAGCGACGACTCCCGGAGGCTGGGGAAGTTCTGCGGCGACGCAGTC
  CCGGGCTCCATCTCCTCCGAAGGGAATGAACTCCTCGTCCAGTTCGTCTCAGATCTCAGT
  GTCACCGCTGATGGCTTCTCAGCCTCCTACAAGACCCTGCCGCGGGGCACTGCCAAAGAA
  GGGCAAGGGCCCGGCCCCAAACGGGGAACTGAGCCTAAAGTCAAGCTGCCCCCCAAGTCC
  CAACCTCCGGAGAAAACAGAGGAATCTCCTTCAGCCCCTGATGCACCCACCTGCCCAAAG
  CAGTGCCGCCGGACAGGCACCTTGCAGAGCAACTTCTGTGCCAGCAGCCTTGTGGTGACT
  GCGACAGTGAAGTCCATGGTTCGGGAGCCAGGGGAGGGCCTTGCCGTGACTGTCAGTCTT
  ATTGGTGCTTATAAAACTGGAGGACTGGACCTGCCTTCTCCACCCACTGGTGCCTCCCTG
  AAGTTTTACGTGCCTTGCAAGCAGTGCCCCCCCATGAAGAAAGGAGTCAGTTATCTGCTG
  ATGGGCCAGGTAGAAGAGAACAGAGGCCCCGTCCTTCCTCCAGAGAGCTTTGTGGTTCTC
  CACCGGCCCAACCAGGACCAGATCCTCACCAACCTAAGCAAGAGGAAGTGCCCCTCTCAA
  CCTGTGCGGGCTGCTGCGTCCCAGGACTGA
  
  Clone variation with respect to NM_002593.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002593 unedited
  TCANATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGGGCCATGC
  TGCCTGCAGCCACAGCCTCCCTCCTGGGGCCCCTCCTCACTGCCTGCGCCCTGCTGCCTT
  TTGCCCAGGGCCAGACCCCCAACTACACCAGACCCGTGTTCCTGTGCGGAGGGGATGTGA
  AGGGGGAATCAGGTTACGTGGCAAGTGAGGGGTTCCCCAACCTCTACCCCCCTAATAAGG
  AGTGCATCTGGACCATAACGGTCCCCGAGGGCCAGACTGTGTCCCTCTCATTCCGAGTCT
  TCGACCTGGAGCTGCACCCCGCCTGCCGCTACGATGCTCTGGAGGTCTTCGCTGGGTCTG
  GGACTTCCGGCCAGCGGCTCGGACGCTTTTGTGGGACCTTCCGGCCTGCGCCCCTAGTCG
  CCCCCGGCAACCAGGTGACCCTGAGGATGACGACGGATGAGGGCACAGGAGGACGAGGCT
  TCCTGCTCTGGTACAGCGGGCGGGCCACCTCGGGCACTGAGCACCAATTTTGCGGNGGGC
  GGCTGGAGAAGGCCCAGGGAACCCTGACCACGCCCAACTGGCCCGAGTCCGATTACCCCC
  CGGGCATCAGCTGTTCCTGGCACATCATCGCGCCCCCGGACCAGGTCATCGCGCTGACCT
  TCGAGAAGTTTGACCTGGAGCCGGACACCTACTGCCGCTATGACTCGGTCAGCGTGTTCA
  ACGGAGCCGTGAGCGACGACTCCCGGAGGCTGGNGAAGTTCTGCGGCGACGCAGTCCCGG
  GCTTCATCTCCTCCGAAAGGAATGAACTNCTCGTNNCAGTCGTCTCAGATCTCAGTGTCA
  CCGCTGATGCCTTCTCAGCCTCCTACAAGACCTTGCCGCGGGCACTGCCCAAGAAGGGCA
  GGGCCCGCCCCAAACGNGAACTGACCCTAAGTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002593 unedited
  CCGCAATCTAGTAGTCGGTTTTTTTTTTTTTTTTTTCCTCATTAAGAAACATTTATTTGG
  ATAAGAAAGAAGGCCTGAGGGCTAGGGGCCGGGGCTGGCCTGCGTCTCATTCCTGGGACG
  CATCAGCCCGCACAGGTTGAGAGGGGCACTTCCTCTTGCTTAGGTTGGTGAGGATCTGGT
  CCTGGTTGGGCCGGTGGAGAACCACAAAGCTCTCTGGAGGAAGGACGGGGCCTCTGTTCT
  CTTCTACCTGGCCCATCAGCAGATAACTGACTCCTTTCTTCATGGGGGGGCACTGCTTGC
  AAGGCACGTAAAACTTCAGGGAGGCACCAGTGGGTGGAGAAGGCAGGTCCAGTCCTCCAG
  TTTTATAAGCACCAATAAGACTGACAGTCACGGCAAGGCCCTCCCCTGGCTCCCGAACCA
  TGGACTTCACTGTCGCAGTCACCACAAGGCTGCTGGCACAGAAGTTGCTCTGCAAGGTGC
  CTGTCCGGCGGCACTGCTTTGGGCAGGTGGGTGCATCAGGGGCTGAAGGAGATTCCTCTG
  TTTTCTCCGGAGGTTGGGACTTGAGGGGCAGCTTGACTTTAGGCTCAGTTCCCCGTTTGG
  GGCCGGGCCCTTGCCCTTTTTGGCAGTGCCCCGCGGCAGGGTCTTGTAAGATGCTGAGAA
  GCCATCAGCGGTGACACTGAGATCTGAGACGATCTGGACGATGAGCTCATTCCCTTTTCG
  AGAGATGGAGCCCGGNACTGCGTCGCCGCANTACTTTCCCAGTCCTCCGGAGTCGTCGCT
  CACGGCTCCGTTGACACGCTGACCGATCATAGCGCAGTAGGTGTCCGCTTCAGTCAAACT
  TCTCGAAGTCATCGCGATGACCTGGTCCGGGGCGCGATGATGTGCAGTACATCGATGCCC
  GGGGGTATCGGCTCGGCCATTGGNCGTGTAAGGTTCCTGGCCTCTCCAGCGCCCCGAAAT
  TGTGCTATGCCGAGTTGGCCCCGCTGTCAAATAGTATCTTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002593
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002593.2, NP_002584.1
• RefSeq Size: 1510 bp
• RefSeq ORF: 1350 bp
• Locus ID: 5118
• Cytogenetics: 7q22.1
• Domains: CUB, NTR
• Protein Families: Secreted Protein
• Gene Summary: 'Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]'