OAS2 (NM_002535) Human Untagged Clone

CAT#: SC118582

OAS2 (untagged)-Human 2'-5'-oligoadenylate synthetase 2, 69/71kDa (OAS2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OAS2
• Synonyms: MGC78578
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118582 sequence for NM_002535 edited (data generated by NextGen Sequencing)
  ATGGGAAATGGGGAGTCCCAGCTGTCCTCGGTGCCTGCTCAGAAGCTGGGTTGGTTTATC
  CAGGAATACCTGAAGCCCTACGAAGAATGTCAGACACTGATCGACGAGATGGTGAACACC
  ATCTGTGACGTCCTGCAGGAACCCGAACAGTTCCCCCTGGTGCAGGGAGTGGCCATAGGT
  GGCTCCTATGGACGGAAAACAGTCTTAAGAGGCAACTCCGATGGTACCCTTGTCCTCTTC
  TTCAGTGACTTAAAACAATTCCAGGATCAGAAGAGAAGCCAACGTGACATCCTCGATAAA
  ACTGGGGATAAGCTGAAGTTCTGTCTGTTCACGAAGTGGTTGAAAAACAATTTCGAGATC
  CAGAAGTCCCTTGATGGGTTCACCATCCAGGTGTTCACAAAAAATCAGAGAATCTCTTTC
  GAGGTGCTGGCCGCCTTCAACGCTCTGAGCTTAAATGATAATCCCAGCCCCTGGATCTAT
  CGAGAGCTCAAAAGATCCTTGGATAAGACAAATGCCAGTCCTGGTGAGTTTGCAGTCTGC
  TTCACTGAACTCCAGCAGAAGTTTTTTGACAACCGTCCTGGAAAACTAAAGGATTTGATC
  CTCTTGATAAAGCACTGGCATCAACAGTGCCAGAAAAAAATCAAGGATTTACCCTCGCTG
  TCTCCGTATGCCCTGGAGCTGCTTACGGTGTATGCCTGGGAACAGGGGTGCAGAAAAGAC
  AACTTTGACATTGCTGAAGGCGTCAGAACCGTTCTGGAGCTGATCAAATGCCAGGAGAAG
  CTGTGTATCTATTGGATGGTCAACTACAACTTTGAAGATGAGACCATCAGGAACATCCTG
  CTGCACCAGCTCCAATCAGCGAGGCCAGTAATCTTGGATCCAGTTGACCCAACCAATAAT
  GTGAGTGGAGATAAAATATGCTGGCAATGGCTGAAAAAAGAAGCTCAAACCTGGTTGACT
  TCTCCCAACCTGGATAATGAGTTACCTGCACCATCTTGGAATGTTCTGCCTGCACCACTC
  TTCACGACCCCAGGCCACCTTCTGGATAAGTTCATCAAGGAGTTTCTCCAGCCCAACAAA
  TGCTTCCTAGAGCAGATTGACAGTGCTGTTAACATCATCCGTACATTCCTTAAAGAAAAC
  TGCTTCCGACAATCAACAGCCAAGATCCAGATTGTCCGGGGAGGATCAACCGCCAAAGGC
  ACAGCTCTGAAGACTGGCTCTGATGCCGATCTCGTCGTGTTCCATAACTCACTTAAAAGC
  TACACCTCCCAAAAAAACGAGCGGCACAAAATCGTCAAGGAAATCCATGAACAGCTGAAA
  GCCTTTTGGAGGGAGAAGGAGGAGGAGCTTGAAGTCAGCTTTGAGCCTCCCAAGTGGAAG
  GCTCCCAGGGTGCTGAGCTTCTCTCTGAAATCCAAAGTCCTCAACGAAAGTGTCAGCTTT
  GATGTGCTTCCTGCCTTTAATGCACTGGGTCAGCTGAGTTCTGGCTCCACACCCAGCCCC
  GAGGTTTATGCAGGGCTCATTGATCTGTATAAATCCTCGGACCTCCCGGGAGGAGAGTTT
  TCTACCTGTTTCACAGTCCTGCAGCGAAACTTCATTCGCTCCCGGCCCACCAAACTAAAG
  GATTTAATTCGCCTGGTGAAGCACTGGTACAAAGAGTGTGAAAGGAAACTGAAGCCAAAG
  GGGTCTTTGCCCCCAAAGTATGCCTTGGAGCTGCTCACCATCTATGCCTGGGAGCAGGGG
  AGTGGAGTGCCGGATTTTGACACTGCAGAAGGTTTCCGGACAGTCCTGGAGCTGGTCACA
  CAATATCAGCAGCTCTGCATCTTCTGGAAGGTCAATTACAACTTTGAAGATGAGACCGTG
  AGGAAGTTTCTACTGAGCCAGTTGCAGAAAACCAGGCCTGTGATCTTGGACCCAGCCGAA
  CCCACAGGTGACGTGGGTGGAGGGGACCGTTGGTGTTGGCATCTTCTGGCAAAAGAAGCA
  AAGGAATGGTTATCCTCTCCCTGCTTCAAGGATGGGACTGGAAACCCAATACCACCTTGG
  AAAGTGCCGGTAAAAGTCATCTAA
  
  Clone variation with respect to NM_002535.2
  753 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002535 unedited
  ATACTTACTTGTCGTACGACTCGATATAGGGCGGCCGCGAATTCGGCACCAGGAGTTGGT
  AAGCTCGCTGCAGTGGGTGGAGAGAGGCCTCTAGACTTCAGTTTCAGTTTCCTGGCTCTG
  GGCAGCAGCAAGAATTCCTCTGCCTCCCATCCTACCATTCACTGTCTTGCCGGCAGCCAG
  CTGAGAGCAATGGGAAATGGGGAGTCCCAGCTGTCCTCGGTGCCTGCTCAGAAGCTGGGT
  TGGTTTATCCAGGAATACCTGAAGCCCTACGAAGAATGTCAGACACTGATCGACGAGATG
  GTGAACACCATCTGTGACGTCCTGCAGGAACCCGAACAGTTCCCCCTGGTGCAGGGAGTG
  GCCATAGGTGGCTCCTATGGACGGAAAACAGTCTTAAGAGGCAACTCCGATGGTACCCTT
  GTCCTCTTCTTCAGTGACTTAAAACAATTCCAGGATCAGAAGAGAAGCCAACGTGACATC
  CTCGATAAAACTGGGGATAAGCTGAAGTTCTGTCTGTTCACGAAGTGGTTGAAAAACAAT
  TTCGAGATCCAGAAGTCCCTTGATGGGTTCACCATCCAGGTGTTCACAAAAAATCAGAGA
  ATCTCTTTCGAGGTGCTGGCCGCCTTCAACGCTCTGAGCTTAAATGATAATCCCAGCCCC
  TGGATCTATCGAGAGCTCAAAAGATCCTTGGATAAGACAAATGCCAGTCCTGGTGAGTTT
  GCAGTCTGCTTCACTGAACTCCAGCAGAAGTTTTTTGACAACCGTCCTGAAAACTAAAGG
  ATTTGATCCTCTTGATAAAGCACTGGCATCAACAGTGCCAGAAAAAAATCAAGATTTACC
  CTCCGCTGTCTCCGTATGCCCTGAGCTGCTACGTGTATGCTGGGACAGGGGTGCAGAAAA
  GACAACTTGACATTGCTGAGGCGTCAGACCCGTTCTGGGAGCCTG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002535
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002535.2, NP_002526.2
• RefSeq Size: 3647 bp
• RefSeq ORF: 2064 bp
• Locus ID: 4939
• Cytogenetics: 12q24.13
• Domains: NTP_transf_2
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains an additional segment in the 3' coding region, compared to variant 1, which leads to a premature stop codon. It encodes isoform 2, also known as p69, which has a shorter, distinct C-terminus compared to isoform 1.