Entactin (NID1) (NM_002508) Human Untagged Clone

CAT#: SC118601

NID1 (untagged)-Human nidogen 1 (NID1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NID1
• Synonyms: NID
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118601 sequence for NM_002508 edited (data generated by NextGen Sequencing)
  ATGTTGGCCTCGAGCAGCCGGATCCGGGCTGCGTGGACGCGGGCGCTGCTGCTGCCGCTG
  CTGCTGGCGGGGCCTGTGGGCTGCCTGAGCCGCCAGGAGCTCTTTCCCTTCGGCCCCGGA
  CAGGGGGACCTGGAGCTGGAGGACGGGGATGACTTCGTCTCTCCTGCCCTGGAGCTGAGT
  GGGGCGCTCCGCTTCTACGACAGATCCGACATCGACGCAGTCTACGTCACCACAAATGGC
  ATCATTGCTACGAGTGAACCCCCGGCCAAAGAATCCCATCCCGGGCTCTTCCCACCAACA
  TTCGGTGCAGTCGCCCCTTTCCTGGCGGACTTGGACACGACCGATGGCCTGGGGAAGGTT
  TATTATCGAGAAGACTTATCCCCCTCCATCACTCAGCGAGCAGCAGAGTGTGTCCACAGA
  GGGTTCCCGGAGATCTCTTTCCAGCCTAGTAGCGCGGTGGTTGTCACTTGGGAATCCGTG
  GCCCCCTACCAAGGGCCCAGCAGGGACCCAGACCAGAAAGGCAAGAGAAACACGTTCCAG
  GCTGTTCTAGCCTCCTCTGATTCCAGCTCCTATGCCATTTTCCTTTATCCTGAGGATGGT
  CTGCAGTTCCATACGACATTCTCAAAGAAGGAAAACAACCAAGTTCCTGCCGTGGTTGCA
  TTCAGTCAAGGTTCAGTGGGATTCTTATGGAAGAGCAACGGAGCTTATAACATATTTGCT
  AATGACAGGGAATCAATTGAAAATTTGGCCAAGAGTAGTAACTCTGGGCAGCAGGGTGTC
  TGGGTGTTTGAGATTGGGAGTCCAGCCACCACCAATGGCGTGGTGCCTGCAGACGTGATC
  CTCGGAACTGAAGATGGGGCAGAGTATGATGATGAGGATGAAGATTATGACCTGGCGACC
  ACTCGTCTGGGCCTGGAGGATGTGGGCACCACGCCCTTCTCCTACAAGGCTCTGAGAAGG
  GGAGGTGCTGACACATACAGTGTGCCCAGCGTCCTCTCCCCGCGCCGGGCAGCTACCGAA
  AGGCCCCTTGGACCTCCCACAGAGAGAACCAGGTCTTTCCAGTTGGCAGTGGAGACTTTT
  CACCAGCAGCACCCTCAGGTCATAGATGTGGATGAAGTTGAGGAAACAGGAGTTGTTTTC
  AGCTATAACACGGATTCCCGCCAGACGTGTGCTAACAACAGACACCAGTGCTCGGTGCAC
  GCAGAGTGCAGGGACTACGCCACGGGCTTCTGCTGCAGCTGTGTCGCTGGCTATACGGGC
  AATGGCAGGCAATGTGTTGCAGAAGGTTCCCCCCAGCGAGTCAATGGCAAGGTGAAAGGA
  AGGATCTTTGTGGGGAGCAGCCAGGTCCCCATTGTCTTTGAGAACACTGACCTCCACTCT
  TACGTAGTAATGAACCACGGGCGCTCCTACACAGCCATCAGCACCATTCCCGAGACCGTT
  GGATATTCTCTGCTTCCACTGGCCCCAGTTGGAGGCATCATTGGATGGATGTTTGCAGTG
  GAGCAGGACGGATTCAAGAATGGGTTCAGCATCACCGGGGGTGAGTTCACTCGCCAGGCT
  GAGGTGACCTTCGTGGGGCACCCGGGCAATCTGGTCATTAAGCAGCGGTTCAGCGGCATC
  GATGAGCATGGGCACCTGACCATCGACACGGAGCTGGAGGGCCGCGTGCCGCAGATTCCG
  TTCGGCTCCTCCGTGCACATTGAGCCCTACACGGAGCTGTACCACTACTCCACCTCAGTG
  ATCACTTCCTCCTCCACCCGGGAGTACACGGTGACTGAGCCCGAGCGAGATGGGGCATCT
  CCTTCACGCATCTACACTTACCAGTGGCGCCAGACCATCACCTTCCAGGAATGCGTCCAC
  GATGACTCCCGGCCAGCCCTGCCCAGCACCCAGCAGCTCTCGGTGGACAGCGTGTTCGTC
  CTGTACAACCAGGAGGAGAAGATCTTGCGCTATGCTCTCAGCAACTCCATTGGGCCTGTG
  AGGGAAGGCTCCCCTGATGCTCTTCAGAATCCCTGCTACATCGGCACTCATGGGTGTGAC
  ACCAACGCGGCCTGTCGCCCTGGTCCCAGGACACAGTTCACCTGCGAGTGCTCCATCGGC
  TTCCGAGGAGACGGGCGAACCTGCTATGATATTGATGAATGTTCAGAACAACCCTCAGTG
  TGTGGGAGCCACACAATCTGCAATAATCACCCAGGAACCTTCCGCTGCGAGTGTGTGGAG
  GGCTACCAGTTTTCAGATGAGGGAACGTGTGTGGCTGTCGTGGACCAGCGCCCCATCAAC
  TACTGTGAAACTGGCCTTCATAACTGCGACATACCCCAGCGGGCCCAGTGTATCTACACA
  GGAGGCTCCTCCTACACCTGTTCCTGCTTGCCAGGCTTTTCTGGGGATGGCCAAGCCTGC
  CAAGATGTGGATGAATGCCATCCAAGCCGATGTCACCCTGACGCCTTCTGCTACAACACT
  CCAGGCTCTTTCACGTGCCAGTGCAAACCTGGTTATCAGGGAGACGGCTTCCGTTGCGTG
  CCCGGAGAGGTGGAGAAAACCCGGTGCCAGCACGAGCGAGAACACATTCTCGGGGCAGCG
  GGGGCGACAGACCCACAGCGACCCATTCCTCCGGGGCTGTTCGTTCCTGAGTGCGATGCG
  CACGGGCACTACGCGCCCACCCAGTGCCACGGCAGCACCGGCTACTGCTGGTGCGTGGAT
  CGCGACGGCCGCGAGGTGGAGGGCACCAGGACCAGGCCCGGGATGACGCCCCCGTGTCTG
  AGTACAGTGGCTCCCCCGATTCACCAAGGACCTGCGGTGCCTACCGCCGTGATCCCCTTG
  CCTCCTGGGACCCATTTACTCTTTGCCCAGACTGGGAAGATTGAGCGCCTGCCCCTGGAG
  GGAAATACCATGAGGAAGACAGAAGCAAAGGCGTTCCTTCATGTCCCGGCTAAAGTCATC
  ATTGGACTGGCCTTTGACTGCGTGGACAAGATGGTTTACTGGACGGACATCACTGAGCCT
  TCCATTGGGAGAGCTAGTCTACATGGTGGAGAGCCAACCACCATCATTAGACAAGATCTT
  GGAAGTCCAGAAGGTATCGCTGTTGATCACCTTGGCCGCAACATCTTCTGGACAGACTCT
  AACCTGGATCGAATAGAAGTGGCGAAGCTGGACGGCACGCAGCGCCGGGTGCTCTTTGAG
  ACTGACTTGGTGAATCCCAGAGGCATTGTAACGGATTCCGTGAGAGGGAACCTTTACTGG
  ACAGACTGGAACAGAGATAACCCCAAGATTGAAACTTCCTACATGGACGGCACGAACCGG
  AGGATCCTTGTGCAGGATGACCTGGGCTTGCCCAATGGACTGACCTTCGATGCGTTCTCA
  TCTCAGCTCTGCTGGGTGGATGCAGGCACCAATCGGGCGGAATGCCTGAACCCCAGTCAG
  CCCAGCAGACGCAAGGCTCTCGAAGGGCTCCAGTATCCTTTTGCTGTGACGAGCTACGGG
  AAGAATCTGTATTTCACAGACTGGAAGATGAATTCCGTGGTTGCTCTCGATCTTGCAATT
  TCCAAGGAGACGGATGCTTTCCAACCCCACAAGCAGACCCGGCTGTATGGCATCACCACG
  GCCCTGTCTCAGTGTCCGCAAGGCCATAACTACTGCTCAGTGAACAATGGCGGCTGCACC
  CACCTATGCTTGGCCACCCCAGGGAGCAGGACCTGCCGTTGCCCTGACAACACCTTGGGA
  GTTGACTGTATCGAACAGAAATGA
  
  Clone variation with respect to NM_002508.2
  736 g=>a;2409 a=>g;2421 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002508 unedited
  GCTCCTTTCCACGCTAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGATCTC
  ATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGG
  CACGAGGGTCCTGGCCGCGGGACCGGGTTTGCGGGACCGCAGTTCGGGAACATGTTGGCC
  TCGAGCAGCCGGATCCGGGCTGCGTGGTCTCGGGCGCTGCTGCTGCCGCTGCTGCTGGCG
  GGGCCTGTGGGCTGCCTGAGCCGCCAGGAGCTCTTTCCCTTCGGCCCCGGACAGGGGGAC
  CTGGAGCTGGAGGACGGGGATGACTTCGTCTCTCCTGCCCTGGAGCTGAGTGGGGCGCTC
  CGCTTCTACGACAGATCCGACATCGACGCAGTCTACGTCACCACAAATGGCATCATTGCT
  ACGAGTGAACCCCCGGCCAAAGAATCCCATCCCGGGCTCTTCCCACCAACATTCGGTGCA
  GTCGCCCCTTTCCTGGCGGACTTGGACACGACCGATGGCCTGGGGAAGGTTTATTATCGA
  GAAGACTTATCCCCCTCCATCACTCAGCGAGCAGCAGAGTGTGTCCACAGAGGGTTCCCG
  GAGATCTCTTTCCAGCCTAGTAGCGCGGTGGTTGTCACTTGGGAATCCGTGGCCCCCTAC
  CAAGGGCCCAGCAGGGACCCAGACCAGAAAGGCAAGAGAAACACGTTCCAGGCTGTTCTA
  GCCTCCTCTGATTCCAGCTCCTATGCCATTTTCCTTTATCCTGAGGATGGTCTGCAGTTC
  CATACGACATTCTCAAAAAGGAAAAACACCAAGTTCCTGCCGTGGTTGCATTCAGTCAAG
  GTTCAGTGGGATTCTTATGGAAGAGCNAACGAGCTTATACATATTTGCTAATGACAGGGA
  ATCAATTGAAAATTTGGCNANGATAGTTACTCTGGGCAGCAGGGTGTCTTGGTGTTTGAG
  ATTGGGAGTCAGCCACACCCATGCGGTGTGCCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002508 unedited
  CTTAACTAGGNTTCGTTTTCCGCTCTCTAAGTTCTAGTTTTTTTTTTTTTTTTTTGTGAT
  ACTTTTCATTGAATCATCTCGACTCCTTTATCCCATCCTTTACTGACTCAAACTCCTTAT
  GCTGAACTTTTCAATCCAAAATTTCTATTGTTTTAGGACTCTGGGAAGCAGTTGATAAAA
  AGATAACACGAGCTACTGATATGGTCAGGAGATGTTTTCTAAACAAATTGTAGAAATATA
  AACATGAAATGTGGCAATGATCCCTTTAATTAGATCATTGAGCAATAAAGGATATGAACC
  ATTAGTATAAATATTCAATTCAGTCTTTCCGGATTGTGTTGTTAGATAAGAATACATCCA
  AAAAGGGCCACAGATCGAGGGAGAAACCAAGGAGGGGTGAGGTCATGGTTGAGTGTAAAT
  TGGTTCTGTCATGTGGAGGCCGGAGGTGAGGGACTGTGGTGGGACATCATCTGGTGCCAG
  CCCCATCCTGTACCTCAGATAGGTCTCCGTGAAAAGGAGGGACATCAGTGCAAGCAGTGG
  GGCTAAAGACCAGCACAGCAACAGAGACTAGAACCTTGGATGCAAGTATTTTGGGCAAAG
  AGGAATAATTAGTAATCTTTGGTATAATTTAAGGCACATCANANAAATCCCCTATTCTCA
  AATATCTGACCTACACCTTGAAATTCCTACCATTGGACATTCTTTTGATTTAAGCCCTTT
  ACTAACANATGCCAATATCCTACTGCTCAAATATTGACATTCTATCTGGGAAGGGCTGCT
  CATATCATCCACAAATGCCCATAGATCTCTAGACAGGGTAGTTTTGATGAAAAAGCACGC
  ATGAATATATTATGGGGGTCTTAAACAGNATGATGATGGGAGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002508
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002508.1, NP_002499.1
• RefSeq Size: 4898 bp
• RefSeq ORF: 3744 bp
• Locus ID: 4811
• Cytogenetics: 1q42.3
• Domains: ldl_recept_b, thyroglobulin_1, EGF_CA, NIDO, EGF, EGF, G2F
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008]'