NDUFB3 (NM_002491) Human Untagged Clone

CAT#: SC118618

NDUFB3 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa (NDUFB3), nuclear gene encoding mitochondrial protein


  "NM_002491" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NDUFB3
Synonyms B12; CI-B12; MC1DN25
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_002491 edited
ATGGCCCATGAACATGGACATGAGCATGGACATCATAAAATGGAACTTCCAGATTATAGA
CAATGGAAGATAGAAGGGACACCATTAGAAACTATCCAGAAGAAGCTGGCTGCAAAAGGG
CTAAGGGATCCATGGGGCCGCAATGAAGCTTGGAGATACATGGGTGGCTTTGCAAAGAGT
GTTTCCTTTTCTGATGTATTCTTTAAAGGATTCAAATGGGGATTTGCTGCATTTGTGGTA
GCTGTAGGAGCTGAATATTACCTGGAGTCCCTGAATAAAGATAAGAAGCATCACTGA
>OriGene 5' read for NM_002491 unedited
TGCTGTCAGACATGGCCCATGAACATGGACATGAGCATGGACATCATAAAATGGAACTTC
CAGATTATAGACAATGGAAGATAGAAGGGACACCATTAGAAACTATCCAGAAGAAGCTGG
CTGCAAAAGGGCTAAGGGATCCATGGGGCCGCAATGAAGCTTGGAGATACATGGGTGGCT
TTGCAAAGAGTGTTTCCTTTTCTGATGTATTCTTTAAAGGATTCAAATGGGGATTTGCTG
CATTTGTGGTAGCTGTAGGAGCTGAATATTACCTGGAGTCCCTGAATAAAGATAAGAAGC
ATCACTGAAGATAATACCTGGAAGCATAATNAGTGGTTTCTTAACTCTCCAAAATAAGAT
TTCTTCTCTGTAGCCTACNNTGTCTGGTTTATCCCTTACAGAATATTAGTAAGATTTAAT
CAATTAAAATATATATATATGCCAAAAAAAAAAAAAAAAAACTCGATCTAGATTGCGGCC
>OriGene 3' read for NM_002491 unedited
NNGGTTACGACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTT
GGCATATATATATATTTTAATTGATTAAATCTTACTAATATTCTGTAAGGGATAAACCAG
ACAAGTAGGCTACAGGAGAAGAAATCTTATTTTGGAGAGTTAAGAAACCACTATGATGCT
TCCAGGTATTATCTTCAGTGATGCTTCTTATCTTTATTCAGGGACTCCAGGTAATATTCA
GCTCCTACAGCTACCACAAATGCAGCAAATCCCCATTTGAATCCTTTAAAGAATACATCA
GAAAAGGAAACACTCTTTGCAAAGCCACCCATGTATCTCCAAGCTTCATTGCGGCCCCAT
GGATCCCTTAGCCCTTTTGCAGCCAGCTTCTTCTGGATAGTTTCTAATGGTGTCCCTTCT
ATCTTCCATTGTCTATAATCTGGAAGTTCCATTTTATGATGTCCATGCTCATGTCCATGT
TCATGGGCCATGTCTGACAGCAATCTGACCTCGTGCCGAATTCGCGGCCGCCCTATAGTG
AGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCAC
CGTACACGCCTACCGCCCATTTGCGTCAACGGGGGCGGGGTTATTACGACATTTTGGAAA
GTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGGTGGAGACTTG
GAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGCCCAAACCGCATCA
CCATGGTAATAGCGATGACTAATACGTAGATGTACTGGCAAGTAAGAAAGTCCCGTAAGG
TCATGTACTGGGCATAATGCCCAGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCG
GACTTGGCCTATGATAC
Restriction Sites NotI-NotI     
ACCN NM_002491
Insert Size 500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002491.1, NP_002482.1
RefSeq Size 693 bp
RefSeq ORF 297 bp
Locus ID 4709
Cytogenetics 2q33.1
Protein Families Transmembrane
Protein Pathways Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Gene Summary 'This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency. Alternative splicing results in multiple transcript variants encoding the same protein. Humans have multiple pseudogenes of this gene. [provided by RefSeq, Mar 2012]'
Transcript Variant: This variant (1) lacks an exon in the 5' UTR, compared to variant 2. Variants 1 and 2 encode the same protein.

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