NDUFB3 (NM_002491) Human Untagged Clone

CAT#: SC118618

NDUFB3 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa (NDUFB3), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFB3
• Synonyms: B12; CI-B12; MC1DN25
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002491 edited
  ATGGCCCATGAACATGGACATGAGCATGGACATCATAAAATGGAACTTCCAGATTATAGA
  CAATGGAAGATAGAAGGGACACCATTAGAAACTATCCAGAAGAAGCTGGCTGCAAAAGGG
  CTAAGGGATCCATGGGGCCGCAATGAAGCTTGGAGATACATGGGTGGCTTTGCAAAGAGT
  GTTTCCTTTTCTGATGTATTCTTTAAAGGATTCAAATGGGGATTTGCTGCATTTGTGGTA
  GCTGTAGGAGCTGAATATTACCTGGAGTCCCTGAATAAAGATAAGAAGCATCACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002491 unedited
  TGCTGTCAGACATGGCCCATGAACATGGACATGAGCATGGACATCATAAAATGGAACTTC
  CAGATTATAGACAATGGAAGATAGAAGGGACACCATTAGAAACTATCCAGAAGAAGCTGG
  CTGCAAAAGGGCTAAGGGATCCATGGGGCCGCAATGAAGCTTGGAGATACATGGGTGGCT
  TTGCAAAGAGTGTTTCCTTTTCTGATGTATTCTTTAAAGGATTCAAATGGGGATTTGCTG
  CATTTGTGGTAGCTGTAGGAGCTGAATATTACCTGGAGTCCCTGAATAAAGATAAGAAGC
  ATCACTGAAGATAATACCTGGAAGCATAATNAGTGGTTTCTTAACTCTCCAAAATAAGAT
  TTCTTCTCTGTAGCCTACNNTGTCTGGTTTATCCCTTACAGAATATTAGTAAGATTTAAT
  CAATTAAAATATATATATATGCCAAAAAAAAAAAAAAAAAACTCGATCTAGATTGCGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002491 unedited
  NNGGTTACGACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTT
  GGCATATATATATATTTTAATTGATTAAATCTTACTAATATTCTGTAAGGGATAAACCAG
  ACAAGTAGGCTACAGGAGAAGAAATCTTATTTTGGAGAGTTAAGAAACCACTATGATGCT
  TCCAGGTATTATCTTCAGTGATGCTTCTTATCTTTATTCAGGGACTCCAGGTAATATTCA
  GCTCCTACAGCTACCACAAATGCAGCAAATCCCCATTTGAATCCTTTAAAGAATACATCA
  GAAAAGGAAACACTCTTTGCAAAGCCACCCATGTATCTCCAAGCTTCATTGCGGCCCCAT
  GGATCCCTTAGCCCTTTTGCAGCCAGCTTCTTCTGGATAGTTTCTAATGGTGTCCCTTCT
  ATCTTCCATTGTCTATAATCTGGAAGTTCCATTTTATGATGTCCATGCTCATGTCCATGT
  TCATGGGCCATGTCTGACAGCAATCTGACCTCGTGCCGAATTCGCGGCCGCCCTATAGTG
  AGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCAC
  CGTACACGCCTACCGCCCATTTGCGTCAACGGGGGCGGGGTTATTACGACATTTTGGAAA
  GTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGGTGGAGACTTG
  GAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGCCCAAACCGCATCA
  CCATGGTAATAGCGATGACTAATACGTAGATGTACTGGCAAGTAAGAAAGTCCCGTAAGG
  TCATGTACTGGGCATAATGCCCAGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCG
  GACTTGGCCTATGATAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002491
• Insert Size: 500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002491.1, NP_002482.1
• RefSeq Size: 693 bp
• RefSeq ORF: 297 bp
• Locus ID: 4709
• Cytogenetics: 2q33.1
• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency. Alternative splicing results in multiple transcript variants encoding the same protein. Humans have multiple pseudogenes of this gene. [provided by RefSeq, Mar 2012]'
  Transcript Variant: This variant (1) lacks an exon in the 5' UTR, compared to variant 2. Variants 1 and 2 encode the same protein.