NDUFB6 (NM_002493) Human Untagged Clone

CAT#: SC118621

NDUFB6 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFB6
• Synonyms: B17; CI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002493 edited
  ATGACGGGGTACACTCCGGATGAGAAACTGCGGCTGCAGCAGCTGCGAGAGCTGAGAAGG
  CGATGGCTGAAGGACCAGGAGCTGAGCCCTCGGGAGCCGGTGCTGCCCCCACAGAAGATG
  GGGCCTATGGAGAAATTCTGGAATAAATTTTTGGAGAATAAATCCCCTTGGAGGAAAATG
  GTCCATGGGGTATACAAAAAGAGTATCTTTGTTTTCACTCATGTACTTGTACCTGTCTGG
  ATTATTCATTATTACATGAAGTATCATGTTTCTGAAAAACCATATGGCATAGTTGAAAAG
  AAGTCCAGAATATTCCCTGGTGATACAATTCTGGAGACTGGAGAAGTAATTCCACCAATG
  AAAGAATTTCCTGATCAACATCATTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002493 unedited
  GAGTCGGATTTGTAACCGACTTTACTATAGGGCGGCACGCGCAATTCGGCACGAGGCTTT
  GCAGAGCGGGAGCGCGCTTAAGTAACTAGTCCGTAGTTCGAGGGTGCGCCGTGTCCTTTT
  GCGTTGGTACCAGCGGCGACATGACGGGGTACACTCCGGATGAGAAACTGCGGCTGCAGC
  AGCTGCGAGAGCTGAGAAGGCGATGGCTGAAGGACCAGGAGCTGAGCCCTCGGGAGCCGG
  TGCTGCCCCCACAGAAGATGGGGCCTATGGAGAAATTCTGGAATAAATTTTTGGAGAATA
  AATCCCCTTGGAGGAAAATGGTCCATGGGGTATACAAAAAGAGTATCTTTGTTTTCACTC
  ATGTACTTGTACCTGTCTGGATTATTCATTATTACATGAAGTATCATGTTTCTGAAAAAC
  CATATGGCATAGTTGAAAAGAAGTCCAGAATATTCCCTGGTGATACAATTCTGGAGACTG
  GAGAAGTAATTCCACCAATGAAAGAATTTCCTGATCAACATCATTAAAGATTATGTAAAA
  AGTTAAAAGGCTTATGAGCCTAAGTTTGTTCCTATATTACCATATTTACTGAATTTTCTG
  GAAAAGTAACTTTAATAAAGTTTAATCTCAGAAAANAAAANNNNAANNNAAANNNANNNN
  NNNNANAANAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCGCCTCTAAATTGCGGCCG
  GGGACTTAGTTGTTCCTGAACCAGATCCGGGTTGAATCCTTGTACCCCTTCCCCAGGGCC
  TTTCCGGGCCTGGAAATTTGCCCTTCCGTGCCCCCCAGCCTTGCCCTAAAAAAATAAGTG
  CATAATTTTGCCGCACTGGTGCCCTTCTATAAATAAGGGGTGGAGGGGGTGTGAATGGAA
  CCAAGGGCAATTTTGAAAACACACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002493 unedited
  GGGGTGGGTGTGTATTAAATAAAGCTAACTTATCTAANCCNNGGCNTCCGTTATTANAAC
  TTTCTATCAAGATTTTCTTTTNCCANNAAATTNCAGTNAATTATTGGTAATATAGGGAAC
  AAACTTAGGCTCATAAGCCTTTTAACTTTTTACATAATCTTTAATGATGTTGATCAGGAA
  ATTCTTTCATTGGTGGAATTACTTCTCCAGTCTCCAGAATTGTATCACCAGGGAATATTC
  TGGACTTCTTTTCAACTATGCCATATGGTTTTTCAGAAACATGATACTTCATGTAATAAT
  GAATAATCCAGACAGGTACAAGTACATGAGTGAAAACAAAGATACTCTTTTTGTATACCC
  CATGGACCATTTTCCTCCAAGGGGATTTATTCTCCAAAAATTTATTCCAGAATTTCTCCA
  TAGGCCCCATCTTCTGTGGGGGCAGCACCGGCTCCCGAGGGCTCAGCTCCTGGTCCTTCA
  GCCATCGCCTTCTCAGCTCTCGCAGCTGCTGCAGCCGCAGTTTCTCATCCGGAGTGTACC
  CCGTCATGTCGCCGCTGGTACCAACGCAAAAGGACACGGCGCACCCTCGAACTACGGACT
  AGTTACTTAAGCGCGCTCCCGCTCTGCAAAGCCTCGTGCCGAATTCGCGGCCGCCCTATA
  GTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCC
  CACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGGCGGGTTATTACGACATTTTGGA
  AAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGGTGGAGAC
  TTGGAAATCCCCGTGAATCAAACCGCTATCCACCCCCATTGGTGTACTGGCCAAAACCCC
  ATTACCATGGTTAATAGCGAATGACTAATACCTTTCATGTTCCTGCCCAATAAGTAAAGT
  CCCGCTAAGTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002493
• Insert Size: 650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002493.3, NP_002484.1
• RefSeq Size: 862 bp
• RefSeq ORF: 387 bp
• Locus ID: 4712
• Cytogenetics: 9p21.1
• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).