NDUFS8 (NM_002496) Human Untagged Clone

CAT#: SC118623

NDUFS8 (untagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) (NDUFS8), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFS8
• Synonyms: CI-23k; CI23KD; MC1DN2; TYKY
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118623 sequence for NM_002496 edited (data generated by NextGen Sequencing)
  ATGCGCTGCCTGACCACGCCTATGCTGCTGCGGGCCCTGGCCCAGGCTGCACGTGCAGGA
  CCTCCTGGTGGCCGGAGCCTCCACAGCAGTGCAGTGGCAGCCACCTACAAGTATGTGAAC
  ATGCAGGATCCCGAGATGGACATGAAGTCAGTGACTGACCGGGCAGCCCGCACCCTGCTG
  TGGACTGAGCTCTTCCGAGGCCTGGGCATGACCCTGAGCTACCTGTTCCGGGAACCGGCC
  ACCATCAACTACCCGTTCGAGAAGGGCCCGCTGAGCCCTCGCTTCCGTGGGGAGCATGCG
  CTGCGCCGGTACCCATCCGGGGAGGAGCGTTGCATTGCCTGCAAGCTCTGCGAGGCCATC
  TGCCCCGCCCAGGCCATCACCATCGAGGCTGAGCCAAGAGCTGATGGCAGCCGCCGGACC
  ACCCGCTATGACATCGACATGACCAAGTGCATCTACTGCGGCTTCTGCCAGGAGGCCTGT
  CCCGTGGATGCCATCGTCGAGGGCCCCAACTTTGAGTTCTCCACGGAGACCCATGAGGAG
  CTGCTGTACAACAAGGAGAAGTTGCTCAACAACGGGGACAAGTGGGAGGCCGAGATCGCC
  GCCAACATCCAGGCTGACTACTTGTATCGGTGA
  
  Clone variation with respect to NM_002496.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002496 unedited
  AGGTCGAATTTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACGAGGAGCCTCCG
  CCTCCCGATTGACTGGCCTGCTTGGCAAGGCAAGTAGCGGCGGCGCTTCAAGATGCGCTG
  CCTGACCACGCCTATGCTGCTGCGGGCCCTGGCCCAGGCTGCACGTGCAGGACCTCCTGG
  TGGCCGGAGCCTCCACAGCAGTGCAGTGGCAGCCACCTACAAGTATGTGAACATGCAGGA
  TCCCGAGATGGACATGAAGTCAGTGACTGACCGGGCAGCCCGCACCCTGCTGTGGACTGA
  GCTCTTCCGAGGCCTGGGCATGACCCTGAGCTACCTGTTCCGGGAACCGGCCACCATCAA
  CTACCCGTTCGAGAAGGGCCCGCTGAGCCCTCGCTTCCGTGGGGAGCATGCGCTGCGCCG
  GTACCCATCCGGGGAGGAGCGTTGCATTGCCTGCAAGCTCTGCGAGGCCATCTGCCCCGC
  CCAGGCCATCACCATCGAGGCTGAGCCAAGAGCTGATGGCAGCCGCCGGACCACCCGCTA
  TGACATCGACATGACCAAGTGCATCTACTGCGGCTTCTGCCAGGAGGCCTGTCCCGTGGA
  TGCCATCGTCGAGGGCCCCAACTTTGAGTTCTCCACGGAGACCCATGAGGAGCTGCTGTA
  CAACAAGGAGAAGTTGCTCAACAACGGNGACAAGTGGGAGGCCGAGATCGCCGCCAACAT
  CCAGGCTGACTACTTGTATCGGTGACGCCCCACCGGNNCCCGCAGCCCTGCTGCCCAATA
  AAACCACTCCGACCCCCANAAANANANNAANNNNNNAACAAACAAAAAAAACAANNNNAC
  AAAAAAANAAAAAAAAACACAANAAAAAAAAAAAAACCCCCTTTTAAATGCGGCCGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002496 unedited
  GCCCGCGCCGGCCCCGCCGGCCCNCCCGCCCCCCGGTTTTTCGGCGTGGTTTTTTGGGCA
  GCAGGGCTGCGGGCCGGTGGGGCGTCACCGCTACAAGTAGTCAGCCTGGCTGTTGGCGGC
  GATCTCGGCCTCCCACTTGTCCCCGTTGTTGAGCAACTTCTCCTTGTTGTACAGCAGCTC
  CTCATGGGTCTCCGTGGAGAACTAAATTGGGGCCCTCGACGATGGCATCCACGGGACAGG
  CCTCCTGGCAGAAGCCGCAGTAGATGCACTTGGTCATGTCGATGTCATAGCGGGTGGTCC
  GGCGGCTGCCATCAGCTCTTGGCTCAGCCTCGATGGTGATGGCCTGCGCGGGGCAGATGG
  CCTCGCAGAGCTTGCAGGCAATGCAACGCTCCTCCCCGGATGGGTACCGGCGCAGCGCAT
  GCTCCCCACGGAAGCGAGGGCTCATCGGGCCCTTCTCGAACGGGTATTTGATGGTGGCCG
  GTTCCCGGAACAGGTAGCTCAGGGTCATGCCCACGCCTCTGAAGAGCTCAGTCCACAGCA
  GGGTGCGGGCTGCCCGGTCAGTCACTGACTTCCTGTCCATTTTCGGATCCTGCATTGTTC
  ACATACTTGTTACGTGGCTGTCACTGCACTGCTGCCGAGGCTTCTGCCCACCCATGAGGT
  TCCTGCACGTGCACCCTGGGTCCCCTGCCCCCACCCTCCTACGTCTTGTATATGCCTCGC
  ATCTTGCAAACGCATCCCCTCCTTCGCCTGCCTACCTCGGCCATTCTATCTGTATTGCCG
  ATGCTTCCTTTTTGCCAAATCCTCTGTCGCCCGTTTCTGGACTTGGTTTACAAAATTTTT
  TACTGGTTATACTAATTTATCTTCTATCTTTATTACACCTAACTACCTTATAAGTAGAAC
  CCTCCATCTTTCCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002496
• Insert Size: 850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002496.1, NP_002487.1
• RefSeq Size: 779 bp
• RefSeq ORF: 633 bp
• Locus ID: 4728
• Cytogenetics: 11q13.2
• Domains: fer4
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq, Mar 2010]'