ID2 (NM_002166) Human Untagged Clone

CAT#: SC118791

ID2 (untagged)-Human inhibitor of DNA binding 2, dominant negative helix-loop-helix protein (ID2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ID2
• Synonyms: bHLHb26; GIG8; ID2A; ID2H
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118791 sequence for NM_002166 edited (data generated by NextGen Sequencing)
  ATGAAAGCCTTCAGTCCCGTGAGGTCCGTTAGGAAAAACAGCCTGTCGGACCACAGCCTG
  GGCATCTCCCGGAGCAAAACCCCTGTGGACGACCCGATGAGCCTGCTATACAACATGAAC
  GACTGCTACTCCAAGCTCAAGGAGCTGGTGCCCAGCATCCCCCAGAACAAGAAGGTGAGC
  AAGATGGAAATCCTGCAGCACGTCATCGACTACATCTTGGACCTGCAGATCGCCCTGGAC
  TCGCATCCCACTATTGTCAGCCTGCATCACCAGAGACCCGGGCAGAACCAGGCGTCCAGG
  ACGCCGCTGACCACCCTCAACACGGATATCAGCATCCTGTCCTTGCAGGCTTCTGAATTC
  CCTTCTGAGTTAATGTCAAATGACAGCAAAGCACTGTGTGGCTGA
  
  Clone variation with respect to NM_002166.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002166 unedited
  GATTTTGTAATACGACTTTCTATAGGCGCGCCGCGAATTCGCACGAGGCCGGTGCCATTC
  GCAGCTAGCTCAGCAGGCGGCAGCGGCGGCCTGAGCTTCAGGGACTGCCAGCTCCCTCCC
  GGTCTCGCCTTCCCTCGCGGTCAGCATGAAAGCCTTCAGTCCCGTGAGGTCCGTTAGGAA
  AAACAGCCTGTCGGACCACAGCCTGGGCATCTCCCGGAGCAAAACCCCTGTGGACGACCC
  GATGAGCCTGCTATACAACATGAACGACTGCTACTCCAAGCTCAAGGAGCTGGTGCCCAG
  CATCCCCCAGAACAAGAAGGTGAGCAAGATGGAAATCCTGCAGCACGTCATCAACTACAT
  CTTGGACCTGCAGATCGCCCTGGACTCGCATCCCACTATTGTCAGCCTGCATCACCAGAG
  ACCCGGGCAGAACCAGGCGTNCAGGACGCCGCTGACCACCCTCAACACGGATATCAGCAT
  CCTGTCCTTGCAGGCTTCTGAATTCCCTTCTGAGTTAATGTCAAATGACAGCAAAGCACT
  GTGTGGCTGAATAAGCGGCGTTCATGACTTCTTTTATTCTTTGCACAACAACAACAACAA
  CAAATTCACGGAATCTTTTAAGTGCTGACCTATATTTTTCAACCATCTTCCCCACGACGA
  CAAGTTGAATGGACCTTTTTAAAAAGAAAACAAAAATGGAAGGAACACTAAGAATGATCA
  CTCTTCCCAGGGTGTCCTCTTACTTGCACTGTGATATTCCGTATTTATGAAAAAGACTTT
  TAAATGCCCTTTCTGCAGTTGGAAGGGTCTCCTTTATATACTATTCCCACCATGGGGAAG
  CGAAAACGGTTAACATCACCAGGAATTGCCCAATCTTAGCCAGACTTTGCCCTCTTTTCA
  AAGGTGGAGCCGTGAATACCATNAAGGTATCCAGTTTCCAACCCTTAAAATGAAGGCTTN
  TGGTCACGAAATTCCCTTCTTGACACAACCTTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002166 unedited
  NNNNNNNNCCCCCCCCNCCCGCCCCCNTNCNGGGTTTGCNTTGCCCGGCGCCGCATTTCA
  CGATCGGTTTTTTTTTTTTTTTTTTNTGATTCAGAACTGTTTTATTATAATCTTATACAG
  TCTACATAAATTTGAACTTGCATTTATTTGGGTTCAGCTATAACATAGCATAATATAAAT
  CAAAGCACTGGCCCTCTGAAACAAAGCAGGCACTCACCATTCACTAAACACCCTTGACTT
  ATTTTGTATAAAAGGGTTAAGTTTACCACTCAACTTTCATACAAAGTTCACCATGAATAA
  GCACATCATTACCCTTTTGAATGCAGAAATAGACATCTCTGCCACTATACAACACAACTC
  TAATTAACCAGCTCACAACGGTTTCACCTCAAATAGATATATTTATCCCTAAATATATAC
  ACAGCACTCAGCCGGCTTGTGTCACAAAAGGTAATTTCTGACCAAAAGACTTCATTTCAC
  TGCCCGAACTCCTGGATCCTTCTGCCATTCACGCTCCACCTTTGAAAAAAGGCAAAGTCT
  GCTTACATTGGGCCATTCCTCTGTGATCCTAACGCTCTCGCTCCCCATGGTGGGAATAGT
  ATATAAACAAAATCTTCCACCTGCCGACAGGCCCTCTCCCCAACGCCTTTCTCATACCCA
  CCGAAATATCACAGCCCCACGCAAGAAAACCCCCTGCCAAGATGAACATTCCTAACCCTC
  CCTTCATTCTTTCTTTTTTTTTCAAACCCGGTCCATTCCACCCGTCCCTCCTTGCGAAAG
  GCTCCCCAAATCATCCCCACCCTTCCCCATTCCCCCGAATTCCTCGTTCCCCCCTGTCCC
  CCCCAAAACAAAAAACCCCCCCACCCCGCTCATTCACCACCACCGGCGCTTTGCTGTATT
  TGTCCCCACCCTCAACGGCACTTTAAAACCCGCCAGGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002166
• Insert Size: 1280 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002166.4, NP_002157.2
• RefSeq Size: 1402 bp
• RefSeq ORF: 405 bp
• Locus ID: 3398
• Cytogenetics: 2p25.1
• Domains: HLH
• Protein Families: ES Cell Differentiation/IPS, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq, Aug 2011]'