GYPB (NM_002100) Human Untagged Clone

CAT#: SC118832

GYPB (untagged)-Human glycophorin B (MNS blood group) (GYPB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GYPB
• Synonyms: CD235b; GPB; GYP; GYPA; MNS; PAS-3; SS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002100 edited
  ATGTATGGAAAAATAATCTTTGTATTACTATTGTCAGAAATTGTGAGCATATCAGCATTA
  AGTACCACTGAGGTGGCAATGCACACTTCAACCTCTTCTTCAGTCACAAAGAGTTACATC
  TCATCACAGACAAATGGAGAAACGGGACAACTTGTCCATCGTTTCACTGTACCAGCTCCT
  GTAGTGATAATACTCATTATTTTGTGTGTGATGGCTGGTATTATTGGAACGATCCTCTTA
  ATTTCTTACACTATTCGCCGACTGATAAAGGCATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002100 unedited
  GGAATTTGTAATACGACTCACTATAGGGCGGCACGCGAATTCGCACGAGCAGGAACCAGC
  TCATGATCTCAGGATGTATGAGAAAAATAATCTTTGTATTACTATTGTCAGAAATTGTGA
  GCATATCAGCATTAAGTACCACTGAGGTGGCAATGCACACTTCAACCTCTTCTTCAGTCA
  CAAAGAGTTACATCTCATCACAGACAAATGGAGAAACGGGACAACTTGTCCATCGTTTCA
  CTGTACCAGCTCCTGTAGTGATAATACTCATTATTTTGTGTGTGATGGCTGGTATTATTG
  GAACGATCCTCTTAATTTCTTACACTATTCGCCGACTGATAAAGGCATGAGGATGTGGCC
  TGCATGCTGCCTGATCTTGCCTAGAACCGGCTGCACCTGCTGTTCTCTTGTTTATGCAAA
  CTGGCTGCACCTGCTATTCCTTTGCTTATGCCCCTACCCCTGGCTATCCTAATTCCCTGT
  TCTCCTGCCTCACTATTACTGTATTCTCTACTTCTAAATAAAAATAAAACAAAATACAAA
  CCGTTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTC
  CTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTNCCCAGTGCCTCTCCTGGCCCTGAA
  GTTGCCACTCCAGTGCCCACCAGCCTTGTCCTATTAAAATTAAGTTGCATCATTTTGTCT
  GACTAGGTGTCCTTCTATAATATATGGGGTGGAGGGGGGTGGTATGGAGCAGGGGCAAGT
  TTGGAAAACACCTGTAGGCCTGCCGGGTCTTTGGGACCCAGCTGGATGCATGGCNCATCT
  GGCTACTGCATCTCGCTCCGGGTCAGCGATTTCTGCCTACCCCGAGTTGTGGATTCAGCC
  TGCTGACAGCTCACTATTTGGTTTTAGAAAACGGGTTACATATGGCAGCTGGCTCACTCT
  ATTTAGGGACACCACCTGCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002100 unedited
  GGTTACTATGNAACCGCGGCCGCAATCTAGNGATCAGTTTTTTTTTTTTTTTTTTAACGG
  TTTGTATTTTGTTTTATTTTTATTTAGAAGTAGAGAATACAGTAATAGTGAGGCAGGAGA
  ACAGGGAATTAGGATAGCCAGGGGTAGGGGCATAAGCAAAGGAATAGCAGGTGCAGCCAG
  TTTGCATAAACAAGAGAACAGCAGGTGCAGCCGGTTCTAGGCAAGATCAGGCAGCATGCA
  GGCCACATCCTCATGCCTTTATCAGTCGGCGAATAGTGTAAGAAATTAAGAGGATCGTTC
  CAATAATACCAGCCATCACACACAAAATAATGAGTATTATCACTACAGGAGCTGGTACAG
  TGAAACGATGGACAAGTTGTCCCGTTTCTCCATTTGTCTGTGATGAGATGTAACTCTTTG
  TGACTGAAGAAGAGGTTGAAGTGTGCATTGCCACCTCAGTGGTACTTAATGCTGATATGC
  TCACAATTTCTGACAATAGTAATACAAAGATTATTTTTCCATACATCCTGAGATCATGAG
  CTGGGTCCTGCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAAATCT
  GACGGTTCACTAAACGAGCTCTGCTTATATAGNACCTCCCACCGTACACGCCTAACCGCC
  ATTTGCGTCAACGGGGCGGGGGTTATTACAACATTTTGGGAAAGTCCCGTTGATTTTGGG
  GCCCAACAAAATCCCCTTGACGTCAATGGGGTTGGAGACTTTGGAAATCCCCTGTGGGTA
  AAACCGCTTCCCCGCCCCATTGGTTGTCTTGGCCAAAACCCCTCACCATGGTGAAAACCG
  ATGAACAATCCCTTAAATGTCCGGCCAATTAGGAAAATCCCCTTAGGGCATTGTACCTGG
  GACAATATGCCCGCGGGGGCCTTTTTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002100
• Insert Size: 600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002100.2, NP_002091.1
• RefSeq Size: 526 bp
• RefSeq ORF: 276 bp
• Locus ID: 2994
• Cytogenetics: 4q31.21
• Domains: Glycophorin_A
• Gene Summary: 'Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPB gene consists of 5 exons and has 97% sequence homology with GYPA from the 5' UTR to the coding sequence encoding the first 45 amino acids. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).