DDB1 (NM_001923) Human Untagged Clone

CAT#: SC118946

DDB1 (untagged)-Human damage-specific DNA binding protein 1, 127kDa (DDB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDB1
• Synonyms: DDBA; UV-DDB1; XAP1; XPCE; XPE; XPE-BF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001923, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGTACAACTACGTGGTAACGGCCCAGAAGCCCACCGCCGTGAACGGCTGCGTGACCGGACACTTTA
  CTTCGGCCGAAGACTTAAACCTGTTGATTGCCAAAAACACGAGATTAGAGATCTATGTGGTCACCGCCGA
  GGGGCTTCGGCCCGTCAAAGAGGTGGGCATGTATGGGAAGATTGCGGTCATGGAGCTTTTCAGGCCCAAG
  GGGGAGAGCAAGGACCTGCTGTTTATCTTGACAGCGAAGTACAATGCCTGCATCCTGGAGTATAAACAGA
  GTGGCGAGAGCATTGACATCATTACGCGAGCCCATGGCAATGTCCAGGACCGCATTGGCCGCCCCTCAGA
  GACCGGCATTATTGGCATCATTGACCCTGAGTGCCGGATGATTGGCCTGCGTCTCTATGATGGCCTTTTC
  AAGGTTATTCCACTAGATCGCGATAATAAAGAACTCAAGGCCTTCAACATCCGCCTGGAGGAGCTGCATG
  TCATTGATGTCAAGTTCCTATATGGTTGCCAAGCACCTACTATTTGCTTTGTCTACCAGGACCCTCAGGG
  GCGGCACGTAAAAACCTATGAGGTGTCTCTCCGAGAAAAGGAATTCAATAAGGGCCCTTGGAAACAGGAA
  AATGTCGAAGCTGAAGCTTCCATGGTGATCGCAGTCCCAGAGCCCTTTGGGGGGGCCATCATCATTGGAC
  AGGAGTCAATCACCTATCACAATGGTGACAAATACCTGGCTATTGCCCCTCCTATCATCAAGCAAAGCAC
  GATTGTGTGCCACAATCGAGTGGACCCTAATGGCTCAAGATACCTGCTGGGAGACATGGAAGGCCGGCTC
  TTCATGCTGCTTTTGGAGAAGGAGGAACAGATGGATGGCACCGTCACTCTCAAGGATCTCCGTGTAGAAC
  TCCTTGGAGAGACCTCTATTGCTGAGTGCTTGACATACCTTGATAATGGTGTTGTGTTTGTCGGGTCTCG
  CCTGGGTGACTCCCAGCTTGTGAAGCTCAACGTTGACAGTAATGAACAAGGCTCCTATGTAGTGGCCATG
  GAAACCTTTACCAACTTAGGACCCATTGTCGATATGTGCGTGGTGGACCTGGAGAGGCAGGGGCAGGGGC
  AGCTGGTCACTTGCTCTGGGGCTTTCAAGGAAGGTTCTTTGCGGATCATCCGGAATGGAATTGGAATCCA
  CGAGCATGCCAGCATTGACTTACCAGGCATCAAAGGATTATGGCCACTGCGGTCTGACCCTAATCGTGAG
  ACTGATGACACTTTGGTGCTCTCTTTTGTGGGCCAGACAAGAGTTCTCATGTTAAATGGAGAGGAGGTAG
  AAGAAACCGAACTGATGGGTTTCGTGGATGATCAGCAGACTTTCTTCTGTGGCAACGTGGCTCATCAGCA
  GCTTATCCAGATCACTTCAGCATCGGTGAGGTTGGTCTCTCAAGAACCCAAAGCTCTGGTCAGTGAATGG
  AAGGAGCCTCAGGCCAAGAACATCAGTGTGGCCTCCTGCAATAGCAGCCAGGTGGTGGTGGCTGTAGGCA
  GGGCCCTCTACTATCTGCAGATCCATCCTCAGGAGCTCCGGCAGATCAGCCACACAGAGATGGAACATGA
  AGTGGCTTGCTTGGACATCACCCCATTAGGAGACAGCAATGGACTGTCCCCTCTTTGTGCCATTGGCCTC
  TGGACGGACATCTCGGCTCGTATCTTGAAGTTGCCCTCTTTTGAACTACTGCACAAGGAGATGCTGGGTG
  GAGAGATCATTCCTCGCTCCATCCTGATGACCACCTTTGAGAGTAGCCATTACCTCCTTTGTGCCTTGGG
  AGATGGAGCGCTTTTCTACTTTGGGCTCAACATTGAGACAGGTCTGTTGAGCGACCGTAAGAAGGTGACT
  TTGGGCACCCAGCCCACCGTATTGAGGACTTTTCGTTCTCTTTCTACCACCAACGTCTTTGCTTGTTCTG
  ACCGCCCCACTGTCATCTATAGCAGCAACCACAAATTGGTCTTCTCAAATGTCAACCTCAAGGAAGTGAA
  CTACATGTGTCCCCTCAATTCAGATGGCTATCCTGACAGCCTGGCGCTGGCCAACAATAGCACCCTCACC
  ATTGGCACCATCGATGAGATCCAGAAGCTGCACATTCGCACAGTTCCCCTCTATGAGTCTCCAAGGAAGA
  TCTGCTACCAGGAAGTGTCCCAGTGTTTCGGGGTCCTCTCCAGCCGCATTGAAGTCCAAGACACGAGTGG
  GGGCACGACAGCCTTGAGGCCCAGCGCTAGCACCCAGGCTCTGTCCAGCAGTGTAAGCTCCAGCAAGCTG
  TTCTCCAGCAGCACTGCTCCTCATGAGACCTCCTTTGGAGAAGAGGTGGAGGTGCACAACCTACTTATCA
  TTGACCAACACACCTTTGAAGTGCTTCATGCCCACCAGTTTCTGCAGAATGAATATGCCCTCAGTCTGGT
  TTCCTGCAAGCTGGGCAAAGACCCCAACACTTACTTCATTGTGGGCACAGCAATGGTGTATCCTGAAGAG
  GCAGAGCCCAAGCAGGGTCGCATTGTGGTCTTTCAGTATTCGGATGGAAAACTACAGACTGTGGCTGAAA
  AGGAAGTGAAAGGGGCCGTGTACTCTATGGTGGAATTTAACGGGAAGCTGTTAGCCAGCATCAATAGCAC
  GGTGCGGCTCTATGAGTGGACAACAGAGAAGGAGCTGCGCACTGAGTGCAACCACTACAACAACATCATG
  GCCCTCTACCTGAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTTATGCGCTCAGTGCTGCTGCTTG
  CCTACAAGCCCATGGAAGGAAACTTTGAAGAGATTGCTCGAGACTTTAATCCCAACTGGATGAGTGCTGT
  GGAAATCTTGGATGATGACAATTTTCTGGGGGCTGAAAATGCCTTTAACTTGTTTGTGTGTCAAAAGGAT
  AGCGCTGCCACCACTGACGAGGAGCGGCAGCACCTCCAGGAGGTTGGTCTTTTCCACCTGGGCGAGTTTG
  TCAATGTCTTTTGCCACGGCTCTCTGGTAATGCAGAATCTGGGTGAGACTTCCACCCCCACACAAGGCTC
  GGTGCTCTTCGGCACGGTCAACGGCATGATAGGGCTGGTGACCTCACTGTCAGAGAGCTGGTACAACCTC
  CTGCTGGACATGCAGAATCGACTCAATAAAGTCATCAAAAGTGTGGGGAAGATCGAGCACTCCTTCTGGA
  GATCCTTTCACACCGAGCGGAAGACAGAACCAGCCACAGGTTTCATCGACGGTGACTTGATTGAGAGTTT
  CCTGGATATTAGCCGCCCCAAGATGCAGGAGGTGGTGGCAAACCTACAGTATGACGATGGCAGCGGTATG
  AAGCGAGAGGCCACTGCAGACGACCTCATCAAGGTTGTGGAGGAGCTAACTCGGATCCATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001923 unedited
  TCACTATTTGTATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGCTGGAGGCT
  GCAGGGCTGACGGCTCTCCTGGCTCATTAAGGTTCCCTACGGAGAAATACTGGTTTTGTG
  TTTGAGGTTCGAATTTGTAGAGCAATAGGGGCACTAATAACGTTGGGCAAGCAGACCGGT
  GGGCCCTTTGAAGTCTGAAGAATCAACGCTCAAAAGGGAGGAGTGTCGCGCTCGAGTCCC
  GACGGGCCGCTCCAAGCCTCGACATGTCGTACAACTACGTGGTAACGGCCCAGAAGCCCA
  CCGCCGTGAACGGCTGCGTGACCGGACACTTTACTTCGGCCGAAGACTTAAACCTGTTGA
  TTGCCAAAAACACGAGATTAGAGATCTATGTGGTCACCGCCGAGGGGCTTCGGCCCGTCA
  AAGAGGTGGGCATGTATGGGAAGATTGCGGTCATGGAGCTTTTCAGGCCCAAGGGGGAGA
  GCAAGGACCTGCTGTTTATCTTGACAGCGAAGTACAATGCCTGCATCCTGNAGTATAAAC
  AGAGTGGCGAGAGCATTGACATCATTACGCGAGCCCATGGCAATGTCCAGGACCGCATTG
  GCCGCCCCTCACAGACCGGCATTATTGGCATCATTGACCCTGAGTGCCGGATGATTGGCC
  CTGCGCTCTATGATGGCCTTTTAAGGTTATTCACTAGATCGCGATATTAAGAACTCAAGG
  CCTTCACATCCGCCTGGAGGAACTGCATGCCCATGACAGCCAGCTCCTCATTGGTTGCCC
  AAGCACCTACTATTTGCTTTGGTAACAAGACCCTCAGGGGCGGGACGTAAAAAACTATGA
  GGGGTTTTCCCGAAAAAGGAATTCTATAAAGGCCCTTTGGAAAAAGGAAAATGTCCAACC
  TGAACCTTCCTGGGGAACGCATACCCCAACACCTTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001923 unedited
  TCTTTTTTGGTAACTAGAACAACATTACATCCCCACCTTTTTTTCCAAAAGCAGTTTGCG
  AGACAAAAGGCTGTGGCGTGGGGATCATCCACCATCTCCAGGTTTTACACCCAGGCTACC
  CATGGCTTGGCAGTCAGGCCTCTAGGCCTGATGCTCTCAGAGGCAATAGAAGAAAAGTAA
  AAGGAAGGTCTCACTTCACAGACAATGAAACCCTCCTAACCCTCTTCCCCACTACCCACA
  ACTCCCTACACTGCCAATCTAAATAAAAAGAGGACAATGCATGAGTGTGAGATACCACAT
  ACACACACACACATACACACACACACACGCACAGCTTCCTTTCAGCCAAAGAACTGCAAA
  ATCCTTCCCCGGAAGGAGGACAACTGGCAACACCAATCAAGGCTTGGTGGTCTAAGGTGA
  TGGCTGGAATCATGTGAGACTGGTAAAAATCCAGGGAGAAAATGTTTCACCTTCAGCTCA
  TTCCCAAGTCTCTATGAAGCCCGCCCCACTTCCACATAGGGGAACTGTGGCTCTGGNGGC
  AGCTGGCTTANGGAAAGGCCTCCCATGGCCAAAAAGACNATGGTGGAGAGGAGGGGGAGG
  GCANCANGGCANAGCCTTTGNGGAGGGTCAGCAAAGGGGCCCNCTGCCCCTTGCTAATGG
  ATCCCGAGTAGCTCCTNCACAACCTTGATGAGGTCGTCTGCAGTGGCCTCTCGCTTCATA
  CCGCTGNCATCGTCATACTGTNAGGGTTGCCACCACCTNCTGCATCTTGGGGGCGGCTAT
  ATNCAGNAAACTCTCAATCAAGTCACCGTCGATGAAACCTGTNNGCTGGTCTGTCTTTCC
  GCTCGTGTGAAAGGATCTNCANAANNGATGCTCGATCTTNCCCACACTTTGATGACTTTA
  ATGANTCGATNCTGCATGTCCAGCCAGAAGTTGTACCAGCCTNTGACAGTGAGGTCACAG
  CCTTTAATGCCGNTGACCGTGCCGAAACCACCGAGCCTGGGTGGGGTGGGAGTCTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001923
• Insert Size: 423 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001923.2, NP_001914.2
• RefSeq Size: 4221 bp
• RefSeq ORF: 3423 bp
• Locus ID: 1642
• Cytogenetics: 11q12.2
• Domains: CPSF_A
• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis
• Gene Summary: 'The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins. [provided by RefSeq, May 2012]'