CPT1A (NM_001876) Human Untagged Clone

CAT#: SC119000

CPT1A (untagged)-Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CPT1A
• Synonyms: CPT1; CPT1-L; L-CPT1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119000 sequence for NM_001876 edited (data generated by NextGen Sequencing)
  ATGGCAGAAGCTCACCAAGCTGTGGCCTTTCAGTTCACGGTCACTCCGGACGGGATTGAC
  CTGCGGCTGAGCCATGAAGCTCTTAGACAAATCTATCTCTCTGGACTTCATTCCTGGAAA
  AAGAAGTTCATCAGATTCAAGAACGGCATCATCACTGGCGTGTACCCGGCAAGCCCCTCC
  AGTTGGCTTATCGTGGTGGTGGGCGTGATGACAACGATGTACGCCAAGATCGACCCCTCG
  TTAGGAATAATTGCAAAAATCAATCGGACTCTGGAAACGGCCAACTGCATGTCCAGCCAG
  ACGAAGAACGTGGTCAGCGGCGTGCTGTTTGGCACCGGCCTGTGGGTGGCCCTCATCGTC
  ACCATGCGCTACTCCCTGAAAGTGCTGCTCTCCTACCACGGGTGGATGTTCACTGAGCAC
  GGCAAGATGAGTCGTGCCACCAAGATCTGGATGGGTATGGTCAAGATCTTTTCAGGCCGA
  AAACCCATGTTGTACAGCTTCCAGACATCGCTGCCTCGCCTGCCGGTCCCGGCTGTCAAA
  GACACTGTGAACAGGTATCTACAGTCGGTGAGGCCTCTTATGAAGGAAGAAGACTTCAAA
  CGGATGACAGCACTTGCTCAAGATTTTGCTGTCGGTCTTGGACCAAGATTACAGTGGTAT
  TTGAAGTTAAAATCCTGGTGGGCTACAAATTACGTGAGCGACTGGTGGGAGGAGTACATC
  TACCTCCGAGGACGAGGGCCGCTCATGGTGAACAGCAACTATTATGCCATGGATCTGCTG
  TATATCCTTCCAACTCACATTCAGGCAGCAAGAGCCGGCAACGCCATCCATGCCATCCTG
  CTTTACAGGCGCAAACTGGACCGGGAGGAAATCAAACCAATTCGTCTTTTGGGATCCACG
  ATTCCACTCTGCTCCGCTCAGTGGGAGCGGATGTTTAATACTTCCCGGATCCCAGGAGAG
  GAGACAGACACCATCCAGCACATGAGAGACAGCAAGCACATCGTCGTGTACCATCGAGGA
  CGCTACTTCAAGGTCTGGCTCTACCATGATGGGCGGCTGCTGAAGCCCCGGGAGATGGAG
  CAGCAGATGCAGAGGATCCTGGACAATACCTCGGAGCCTCAGCCCGGGGAGGCCAGGCTG
  GCAGCCCTCACCGCAGGAGACAGAGTTCCCTGGGCCAGGTGTCGTCAGGCCTATTTTGGA
  CGTGGGAAAAATAAGCAGTCTCTTGATGCTGTGGAGAAAGCAGCGTTCTTCGTGACGTTA
  GATGAAACTGAAGAAGGATACAGAAGTGAAGACCCGGATACGTCAATGGACAGCTACGCC
  AAATCTCTACTACACGGCCGATGTTACGACAGGTGGTTTGACAAGTCGTTCACGTTTGTT
  GTCTTCAAAAACGGGAAGATGGGCCTCAACGCTGAACACTCCTGGGCAGATGCGCCGATC
  GTGGCCCACCTTTGGGAGTACGTCATGTCCATTGACAGCCTCCAGCTGGGCTATGCGGAG
  GATGGGCACTGCAAAGGCGACATCAATCCGAACATTCCGTACCCCACCAGGCTGCAGTGG
  GACATCCCGGGGGAATGTCAAGAGGTTATAGAGACCTCCCTGAACACCGCAAATCTTCTG
  GCAAACGACGTGGATTTCCATTCCTTCCCATTCGTAGCCTTTGGTAAAGGAATCATCAAG
  AAATGTCGCACGAGCCCAGACGCCTTTGTGCAGCTGGCCCTCCAGCTGGCGCACTACAAG
  GACATGGGCAAGTTTTGCCTCACATACGAGGCCTCCATGACCCGGCTCTTCCGAGAGGGG
  AGGACGGAGACCGTGCGCTCCTGCACCACTGAGTCATGCGACTTCGTGCGGGCCATGGTG
  GACCCGGCCCAGACGGTGGAACAGAGGCTGAAGTTGTTCAAGTTGGCGTCTGAGAAGCAT
  CAGCATATGTATCGCCTCGCCATGACCGGCTCTGGGATCGATCGTCACCTCTTCTGCCTT
  TACGTGGTGTCTAAATATCTCGCTGTGGAGTCCCCTTTCCTTAAGGAAGTTTTATCTGAG
  CCTTGGAGATTATCAACAAGCCAGACCCCTCAGCAGCAAGTGGAGCTGTTTGACTTGGAG
  AATAACCCAGAGTACGTGTCCAGCGGAGGGGGCTTTGGACCGGTTGCTGATGACGGCTAT
  GGTGTGTCGTACATCCTTGTGGGAGAGAACCTCATCAATTTCCACATTTCTTCCAAGTTC
  TCTTGCCCTGAGACGGATTCTCATCGCTTTGGAAGGCACCTGAAAGAAGCAATGACTGAC
  ATCATCACTTTGTTTGGTCTCAGTTCTAATTCCAAAAAGTAA
  
  Clone variation with respect to NM_001876.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001876 unedited
  ACCCCGCCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG
  AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA
  TTCGGCACGAGGCCGGCGTCGGGTGCGCTCGGCCTCGCCCGCGGCCCTCCTTCCCCGGCT
  CCCGCTCGCCGCTCGTTCACTCCACCGCCGCCGCCGCCGCCGCCGCTGCCGCTGCCGCTG
  CCGCACCTCCGTAGCTGACTCGGTACTCTCTGAAGATGGCAGAAGCTCACCAAGCTGTGG
  CCTTTCAGTTCACGGTCACTCCGGACGGGATTGACCTGCGGCTGAGCCATGAAGCTCTTA
  GACAAATCTATCTCTCTGGACTTCATTCCTGGAAAAAGAAGTTCATCAGATTCAAGAACG
  GCATCATCACTGGCGTGTACCCGGCAAGCCCCTCCAGTTGGCTTATCGTGGTGGTGGGCG
  TGATGACAACGATGTACGCCAAGATCGACCCCTCGTTAGGAATAATTGCAAAAATCAATC
  GGACTCTGGAAACGGCCAACTGCATGTCCAGCCAGACGAAGAACGTGGTCAGCGGCGTGC
  TGTTTGGCACCGGCCTGTGGGTGGCCCTCATCGTCACCATGCGCTACTCCCTGAAAGTGC
  TGCTCTCCTACCACGGGTGGATGTTCACTGAGCACGGNCAGATGAGTCGTGCCACCAAGA
  TCTGGATGGGTATGGTCAAGATCTTTTCANGCCGAANACCCATGTTGTACAGCTTCCAGA
  CATCGCTGCCTCGCCTGCCGGTCCCGGCTGTCAAGACACTGTGAACAGGTATCTACAGTC
  GGTGAAGCCCTCTATGAAGGAAGAAAGACTCANACGGATGACAGCACTTGCTCAGAATTN
  TGCTGTCGTCTTGGACCAGATACAGTGTATTTGAGTTAAATCCTGTGGCTACAATACTGA
  GCACTGTGGGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001876 unedited
  CGCCTACTAGTGTACGTTCCGCNCTCNANGATCNATTTTTTTTTTTTTTTTTTGTCTTTT
  GTTTTCATTTTTTCTTGCTTTCAGGGAGAAAAAAAATCTTTGGCCCATAAAGGTGGGATT
  TTTACATCATGAAGTATACATACATAAAACATGCATGTGGGCCGCGCATGGTGGCTCATG
  CCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTCG
  AGACCAGCCTGACCAATATGATGAAACCCCGTCTCTACTAAAAATACAAAAATCAGCAGG
  GCATGGTGTTGGGCGTGTAATCCCAGCCACTCGGGAGGCTGAGACAGGAGAATCGCTTGA
  ACCTGCAAGGCAGATGCTGCAGTGAGCCGAGATCGCACCACTGCACTCCAGCCTGGGCAA
  CAAGAGCGAAACTCCATCTCAAAAAAAAAAAAAAAAAAAAAAGGCACGTGTTCTCCGGCA
  AGCACCTTCCATCGACTCCAACAGTTCAGCCATCGCTGTTGTACTATCGGGGTGCAGCAC
  TCTGAAGGAGTCAGGCCCAACTAGAACCAAGTGGTCAAAAAAATAAATACACAATTTAAA
  ACAATGGACAGCTAAGCTTGTCATGACAGACAGTGGGAATTTCATTCCATGATGCTTCAT
  TTTTGCATTAAATACTGAAGATGACTCAGTGCATAATTGCTTTGTGGNTCATGAGACGCC
  TTCGTGAAGGCACTCCTATCTGAAGGACAGGCTGGGTGAACACCTAGCACCGTCACTTGA
  GACATCTTGGCTGGTCACCAAGTGAGCCCGGCTACTGTCTCCATCACCCTCTGGTGAGGT
  CTTCCCCTCACCCGGTGGGAAGAGAAAGCCATCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001876
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001876.2, NP_001867.2
• RefSeq Size: 2941 bp
• RefSeq ORF: 2322 bp
• Locus ID: 1374
• Cytogenetics: 11q13.3
• Domains: Carn_acyltransf
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, PPAR signaling pathway
• Gene Summary: 'The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).