CKS1 (CKS1B) (NM_001826) Human Untagged Clone

CAT#: SC119010

CKS1B (untagged)-Human CDC28 protein kinase regulatory subunit 1B (CKS1B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CKS1B
• Synonyms: CKS1; ckshs1; PNAS-16; PNAS-18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001826 edited
  ATGTCGCACAAACAAATTTACTATTCGGACAAATACGACGACGAGGAGTTTGAGTATCGA
  CATGTCATGCTGCCCAAGGACATAGCCAAGCTGGTCCCTAAAACCCATCTGATGTCTGAA
  TCTGAATGGAGGAATCTTGGCGTTCAGCAGAGTCAGGGATGGGTCCATTATATGATCCAT
  GAACCAGAACCTCACATCTTGCTGTTCCGGCGCCCACTACCCAAGAAACCAAAGAAATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001826 unedited
  TAGATTTGTATACGACNACTATAGGCGGCCGCGNAATTCGCACGAGGGTTGGGAGTTGCT
  TGGAGGTTGGCGGCGCGGGGCTGAAGGCTAGCAAACCGAGCGATCATGTCGCACAAACAA
  ATTTACTATTCGGACAAATACGACGACGAGGAGTTTGAGTATCGACATGTCATGCTGCCC
  AAGGACATAGCCAAGCTGGTCCCTAAAACCCATCTGATGTCTGAATCTGAATGGAGGAAT
  CTTGGCGTTCAGCAGAGTCAGGGATGGGTCCATTATATGATCCATGAACCAGAACCTCAC
  ATCTTGCTGTTCCGGCGCCCACTACCCAAGAAACCAAAGAAATGAAGCTGGCAAGCTACT
  TTTCAGCCTCAAGCTTTACACAGCTGTCCTTACTTCCTAACATCTTTCTGATAACATTAT
  TATGTTGCCTTCTTGTTTCTCACTTTGATATTTAAAAGATGTTCAATACACTGTTTGAAT
  GTGCTGGTAACTGCTTTGCTTCTTGAGTAGAGCCACCACCACCATAGCCCAGCCAGATGA
  GTGCTCTGTGGACCCACAGCCTAAGCTGAGTGTGACCCCAGAAGCCACGATGTGCTCTGT
  ATCCAGAACACACTTGGCAGATGGAGGAAGCATCTGAGTTTGAGACCATGGCTGTTACAG
  GGATCATGTAAACTTGCTGTTTTTGTTTTTTCCTGCCGGGTGTTGTATGTGTGGTGACTT
  GCGGATTTATGTTTCAGTGTACTGGAAACTTTNCATTNTATTCAAGAAATCTGTTCATGT
  TAAAAGCCTTGATTAAAGAGGAAGTTTTTATAATCTAGTGCTGTAATTGTACGGGTTTTT
  TTCCCCTCACTCAGGGTGAGCATCATCAATATCATGGGTCTAANTATATGCCCCTTNCAG
  TTGATAATGAAGCTCAGCTCTTTGCTGATAATGAGGGTTAACATTGAGATACTGGNTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001826
• Insert Size: 2630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001826.1, NP_001817.1
• RefSeq Size: 717 bp
• RefSeq ORF: 240 bp
• Locus ID: 1163
• Cytogenetics: 1q21.3
• Domains: CKS
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Pathways in cancer, Small cell lung cancer
• Gene Summary: 'CKS1B protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS1B mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects a specialized role for the encoded protein. At least two transcript variants have been identified for this gene, and it appears that only one of them encodes a protein. [provided by RefSeq, Sep 2008]'
  Transcript Variant: This variant (1) is the protein-coding variant.