Cyclin D2 (CCND2) (NM_001759) Human Untagged Clone

CAT#: SC119036

CCND2 (untagged)-Human cyclin D2 (CCND2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCND2
• Synonyms: KIAK0002; MPPH3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119036 sequence for NM_001759 edited (data generated by NextGen Sequencing)
  ATGGAGCTGCTGTGCCACGAGGTGGACCCGGTCCGCAGGGCCGTGCGGGACCGCAACCTG
  CTCCGAGACGACCGCGTCCTGCAGAACCTGCTCACCATCGAGGAGCGCTACCTTCCGCAG
  TGCTCCTACTTCAAGTGCGTGCAGAAGGACATCCAACCCTACATGCGCAGAATGGTGGCC
  ACCTGGATGCTGGAGGTCTGTGAGGAACAGAAGTGCGAAGAAGAGGTCTTCCCTCTGGCC
  ATGAATTACCTGGACCGTTTCTTGGCTGGGGTCCCGACTCCGAAGTCCCATCTGCAACTC
  CTGGGTGCTGTCTGCATGTTCCTGGCCTCCAAACTCAAAGAGACCAGCCCGCTGACCGCG
  GAGAAGCTGTGCATTTACACCGACAACTCCATCAAGCCTCAGGAGCTGCTGGAGTGGGAA
  CTGGTGGTGCTGGGGAAGTTGAAGTGGAACCTGGCAGCTGTCACTCCTCATGACTTCATT
  GAGCACATCTTGCGCAAGCTGCCCCAGCAGCGGGAGAAGCTGTCTCTGATCCGCAAGCAT
  GCTCAGACCTTCATTGCTCTGTGTGCCACGGACTTTAAGTTTGCCATGTACCCACCGTCG
  ATGATCGCAACTGGAAGTGTGGGAGCAGCCATCTGTGGGCTCCAGCAGGATGAGGAAGTG
  AGCTCGCTCACTTGTGATGCCCTGACTGAGCTGCTGGCTAAGATCACCAACACAGACGTG
  GATTGTCTCAAAGCTTGCCAGGAGCAGATTGAGGCGGTGCTCCTCAATAGCCTGCAGCAG
  TACCGTCAGGACCAACGTGACGGATCCAAGTCGGAGGATGAACTGGACCAAGCCAGCACC
  CCTACAGACGTGCGGGATATCGACCTGTGA
  
  Clone variation with respect to NM_001759.3
  570 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001759 unedited
  TACGACTCTCTATAGGGCGGCCGCGAATTCGGCACGAGGGTGCGAGTGAGGCAGCCCCGA
  GGCTCTGCTCGCCCACCACCCAATCCTCGCCTCCCTTCTGCTCCACCTTCTCTCTCTGCC
  CTCACCTCTCCCCCGAAAACCCCCTATTTAGCCAAAGGAAGGAGGTCAGGGGAACGCTCT
  CCCCTCCCCTTCCAAAAAACAAAAACAGAAAAACCCTTTTCCAGGCCGGGGAAAGCAGGA
  GGGAGAGGGGCCGCCGGGCTGGCCATGGAGCTGCTGTGCCACGAGGTGGACCCGGTCCGC
  AGGGCCGTGCGGGACCGCAACCTGCTCCGAGACGACCGCGTCCTGCAGAACCTGCTCACC
  ATCGAGGAGCGCTACCTTCCGCAGTGCTCCTACTTCAAGTGCGTGCAGAAGGACATCCAA
  CCCTACATGCGCAGAATGGTGGCCACCTGGATGCTGGAGGTCTGTGAGGAACAGAAGTGC
  GAAGAAGAGGTCTTCCCTCTGGCCATGAATTACCTGGACCGTTTCTTGGCTGGNGTCCCG
  ACTCCGAAGTCCCATCTGCAACTCCTGGGTGCTGTCTGCATGTTCCTGGCCTCCAAACTC
  AAGAGACCAGCCCGCTGNACCGCGAGAAGCTGTGCATTTACACCCGACACTNCATTCAGC
  CTCAAGAGCTGCTGGAGTGGAAACTGGTGTGCTGGGGAAGTTGAAGTGGAAACCTGCACC
  TGTCACTCCTCATGAACTCATTGAGCACATTTTTGCCCAAGCTGGCCCCACACCGGGAGA
  AACTGTTTCTGGATCGCAAGCATGCTTCAAACCTCATTGGTCTTGGGGGCCACGGCCTTT
  AGTTTGCATGGCCCCCCCGCATGATCCCACTGTAATGGGGGAACACCCTTGGGGGCCTCA
  CCAGAGAGGAAGGACCCCCTCATGGGAGCCCTCACTGACGCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001759 unedited
  GCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTGCTGTACTTCAAATAGGCACCAA
  ATGCTTTTTATTATTCCGTTTTCGTAGGACCTTTTTCTCTCACTTCTAAAAGATCTTTAA
  ATATGATCTAAGTGGGGGCAGAATTTTTTTTTTAAGTTTCACCCTAAAACACAAAGAACA
  AAAACAACCAGAAAGAAGAAGAGACCAGATTATGGACGCGTCTCTCTCTTTCGGCCCAAC
  TGGCATCCTCACAGGTCGATATCCCGCACGTCTGTAAGGGTGCTGGCTTGGTCCAGTTCA
  TCCTCCGACTTGGATCCGTCACGTTGGTCCTGACGGTACTGCTGCAGGCTATTGAGGAGC
  ACCGCCTCAATCTGCTCCTGGCAAGCTTTGAGACAATCCACGTCTGTGTTGGTGATCTTA
  GCCAGCAGCTCAGTCAGGGCATCACAAGTGAGCGAGCTCACTTCCTCATCCTGCTGGAGC
  CCACAGATGGCTGCTCCCACACTTCCAGTTGCGATCATCGACGGTGGGTACATGGCAAAC
  TTAAAGTCCGTGGCACACAGAGCAATGAAAGTCTGAGCATGCTTGCGGATCAGAGACAGC
  TTCTCCCGCTGCTGGGGCAGCTTGCGCAAGATGTGCTCAATGAAGTCATGAGGAGTGACA
  GCTGCCAGGTTCCACTTAAATTACCCAGCACAACAGTTCCACTTCAGCAGCTCCTGAAGC
  TTGAGGAGTTGTCCGGGTATATGCCAAGTTCTCCGCGGGCAACGGGGCTGGTTTTCTTTG
  ATTTTGAAGGCCCAGAACATGCAAACACGACCCAGAAATTTGCAATGGTATTTTGGAATT
  CGGGACCCCATCCATAAAACGGTCACGCTATTCATGGGCCAGAGGGAAAACCCTCTTTTT
  GCACTCTTGGTCCTTACATAATCTCAATCCCCAGGTGGCACCATTCTGCCCAGGAGCGGG
  GGATTGCCTTTCCGCCCCCTTGAAGAAGAGCCCTGGCAAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001759
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001759.2, NP_001750.1
• RefSeq Size: 6480 bp
• RefSeq ORF: 870 bp
• Locus ID: 894
• Cytogenetics: 12p13.32
• Domains: cyclin_C, CYCLIN, cyclin
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Focal adhesion, Jak-STAT signaling pathway, p53 signaling pathway, Wnt signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3). [provided by RefSeq, Sep 2014]'