BPGM (NM_001724) Human Untagged Clone

CAT#: SC119062

BPGM (untagged)-Human 2,3-bisphosphoglycerate mutase (BPGM), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BPGM
• Synonyms: DPGM; ECYT8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119062 sequence for NM_001724 edited (data generated by NextGen Sequencing)
  ATGTCCAAGTACAAACTTATTATGTTAAGACATGGAGAGGGTGCTTGGAATAAGGAGAAC
  CGTTTTTGTAGCTGGGTGGATCAGAAACTCAACAGCGAAGGAATGGAGGAAGCTCGGAAC
  TGTGGGAAGCAACTCAAAGCGTTAAACTTTGAGTTTGATCTTGTATTCACATCTGTCCTT
  AATCGGTCCATTCACACAGCCTGGCTGATCCTGGAAGAGCTAGGCCAGGAATGGGTGCCT
  GTGGAAAGCTCCTGGCGTCTAAATGAGCGTCACTATGGGGCCTTGATCGGTCTCAACAGG
  GAGCAGATGGCTTTGAATCATGGTGAAGAACAAGTGAGGCTCTGGAGAAGAAGCTACAAT
  GTAACCCCGCCTCCCATTGAGGAGTCTCATCCTTACTACCAAGAAATCTACAACGACCGG
  AGGTATAAAGTATGCGATGTGCCCTTGGATCAACTGCCACGGTCGGAAAGCTTAAAGGAT
  GTTCTGGAGAGACTCCTTCCCTATTGGAATGAAAGGATTGCTCCCGAAGTATTACATGGC
  AAAACCATTCTGATATCTGCTCATGGAAATAGCAGTAGGGCACTCCTAAAACACCTGGAA
  GGTATCTCAGATGAAGACATCATCAACATTACTCTTCCTACTGGAGTCCCCATTCTTCTG
  GAATTGGATGAAAACCTGCGTGCTGTTGGGCCTCATCAGTTCCTGGGTGACCAAGAGGCG
  ATCCAAGCAGCCATTAAGAAAGTAGAAGATCAAGGAAAAGTGAAACAAGCTAAAAAATAG
  
  Clone variation with respect to NM_001724.4
  536 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001724 unedited
  TACGACTCTCTATAGGCGGTCGCGAATTCGGCACGAGGTGCTGTCCTTGAATATTAGCCC
  ATTTGAAAACGCCTGGGAAGTTCAGCCATCAGTATGTCCAAGTACAAACTTATTATGTTA
  AGACATGGAGAGGGTGCTTGGAATAAGGAGAACCGTTTTTGTAGCTGGGTGGATCAGAAA
  CTCAACAGCGAAGGAATGGAGGAAGCTCGGAACTGTGGGAAGCAACTCAAAGCGTTAAAC
  TTTGAGTTTGATCTTGTATTCACATCTGTCCTTAATCGGTCCATTCACACAGCCTGGCTG
  ATCCTGGAAGAGCTAGGCCAGGAATGGGTGCCTGTGGAAAGCTCCTGGCGTCTAAATGAG
  CGTCACTATGGGGCCTTGATCGGTCTCAACAGGGAGCAGATGGCTTTGAATCATGGTGAA
  GAACAAGTGAGGCTCTGGAGAAGAAGCTACAATGTAACCCCGCCTCCCATTGAGGAGTCT
  CATCCTTACTACCAAGANATCTACAACGACCGGAGGTATAAAGTATGCGATGTGCCCTTG
  GATCAACTGCCACGGTCGGAAAGCTTCTAGGATGTTCTGGAGAGACTCCTTCCCTATTGG
  AATGAAAGGATTGCTCCCGAAGTATTACATGGCAAAACCATTCTGATATCTGCTCATGGA
  AATAGCAGTAGGGCACTCCTAAAACACCTGGAAGGTATCTCAGATGAAGACATCATCAAC
  ATTACTCTTTCTACTGGAGTCCCCATTCTTCTGAAATTGGATGAAAACCTGCGTGCTGTT
  GGGCCTCATCAGGTCCTGGGTGACCCAAGAGGCGATCCAAGCCGCCTTTTAAGAAGTNNA
  AGATCCAGGAAAAGTGGAACCAGCTAAAAAATAGCCTTTCTCAACTGTTGCCTAAAAAAA
  ATGCCAAAGAGTGGCCTAGGAATGGGGTATGGGGGCTGACTTTTCTTTTTTTCCCCGATT
  TCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001724 unedited
  CGGCCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGCCATTTTCAGTCACTTTAT
  TATCAGGAACTTTATTTTTAAACTAATTACAGGTACCCAAAGTTCAAAAAATTAAAACAA
  ACCAAAATGAGAACCTCAACAGCCATAGTTGTTTATTTGCTGCTTATTCTTGTGGCCAAA
  GAGGAATTCTACTAAATGTCAAATGAAGGATGTCTCTAAACTAGAAGACAAGTTAAAGGG
  CCTTGTTGTCATTTCTGTCATTGTGGTGGTGGTTGTTGGCTTTCTTGGAGAAATATATCT
  GTGACTTTCTCAACTAATGGGCCTCTGGCCTTAATTATCAAAGTATCCCTTTTCCTAGAG
  GAAAAGACTAGTAATAAATACATACGGGATATTATCTAATATCCCTTACTGTTATCCCAG
  AAAATCTTATTAAATATTAAAGTGAACTACTGATTAGAATAGTGCCTGTCAATGGTGACT
  CAATAATGATTTCTTGAATGATAAATACCTTTAACTTCATCTCTACTTGTTGCTCTCCCA
  TCTCAGAAACTTATGACCTTCATTAAGCCCCACTAGTTACTAGTTTGGTGACCGACTAGG
  GGGCTAGCAAGAAAGCTAACTCATAAGGCTATGACATAAGCACTGAGGCATCCTAAAGCC
  TTATCTGGGCCACAATAAGTTACCTAGTTACCTATACAAACTCTACTCCACAGCCTACCT
  CCTGAAAATCGGGGAAAAAGAGAGAGAGTTCAGCACCCATAACACACTCCTATGCCACTT
  CTTTTGCATTTCTTCTTACCACAGTTGAGAAGACTATTTTTAGCTGGNTTCACTTTTCCT
  GAACTTCACTTTCTTAGGCCTGCTNGAACCGCTTTTGTCCCCCCGAACTGATAAGCCCAC
  CGCCCCCAGGTTTCTACAATCCACAAAAAGGGACCCCCTAGAAAAGACGTCGAAATGGTT
  CTTTCAAAACTTCAGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001724
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001724.3, NP_001715.1
• RefSeq Size: 1769 bp
• RefSeq ORF: 780 bp
• Locus ID: 669
• Cytogenetics: 7q33
• Domains: PGAM
• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways
• Gene Summary: '2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1, 2, and 3 encode the same protein.