CHAD (NM_001267) Human Untagged Clone

CAT#: SC119347

CHAD (untagged)-Human chondroadherin (CHAD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHAD
• Synonyms: SLRR4A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119347 sequence for NM_001267 edited (data generated by NextGen Sequencing)
  ATGGTCCGCCCAATGCTCTTGCTCAGCCTCGGCCTCCTGGCTGGTCTGCTGCCGGCGCTG
  GCCGCCTGCCCCCAGAACTGCCACTGCCACAGCGACCTGCAGCACGTCATCTGCGACAAG
  GTGGGGCTGCAGAAGATCCCCAAGGTGTCAGAGAAGACCAAGCTGCTCAACCTACAGCGC
  AACAACTTCCCGGTGCTGGCTGCCAATTCGTTCCGGGCCATGCCGAACCTCGTGTCATTG
  CACCTGCAGCACTGCCAGATCCGCGAGGTGGCCGCCGGTGCCTTCCGCGGCCTCAAGCAA
  CTTATCTACTTGTACCTGTCCCATAACGACATCCGCGTGCTGCGCGCAGGTGCCTTCGAC
  GACCTGACCGAGCTGACCTACCTCTACCTGGACCACAACAAGGTCACTGAGCTGCCCCGG
  GGGTTGCTCTCCCCGCTGGTCAACCTCTTCATCTTGCAGCTCAACAACAACAAGATCCGT
  GAGCTGCGCGCAGGCGCCTTCCAGGGAGCCAAGGACCTGCGCTGGCTCTACCTGTCGGAA
  AACGCGTTGAGCTCCCTGCAGCCCGGGGCCCTGGACGATGTGGAGAACCTCGCCAAATTC
  CACGTGGACAGGAACCAGCTGTCCAGCTACCCCTCAGCTGCCCTGAGCAAGCTACGGGTG
  GTGGAGGAGCTGAAGCTGTCCCACAACCCCCTGAAAAGCATCCCGGACAATGCCTTCCAG
  TCCTTTGGCAGATACCTGGAGACCCTCTGGCTGGACAACACCAACCTGGAGAAGTTCTCA
  GATGGTGCCTTCCTGGGTGTAACCACGCTGAAACACGTCCATTTGGAGAACAACCGCTTG
  AACCAGCTACCCTCCAACTTCCCCTTCGACAGCCTGGAGACCCTCGCCCTTACCAATAAC
  CCCTGGAAGTGTACCTGCCAGCTCCGGGGCCTTCGGCGGTGGCTGGAAGCCAAGGCCTCC
  CGCCCAGATGCCACCTGTGCCTCACCTGCCAAGTTCAAGGGCCAGCACATCCGTGACACG
  GACGCCTTCCGCAGCTGCAAGTTCCCCACCAAGAGGTCCAAGAAAGCTGGCCGCCATTAA
  
  Clone variation with respect to NM_001267.2
  579 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001267 unedited
  GCAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCTCGAGCT
  CCACGTAGAGCTGGCTCGGCTGCCGGCTGCGGTCAGGGCCACCGTATAAAGAGGGCGGCA
  GACCCGAGCGCCTAGGACTCGCCGCTGCCCGCGCCCCGCCGCCGCTGCTGCCCCCAGCCC
  CGGCCCCAGGCGTCCCAGCCATGGTCCGCCCAATGCTCTTGCTCAGCCTCGGCCTCCTGG
  CTGGTCTGCTGCCGGCGCTGGCCGCCTGCCCCCAGAACTGCCACTGCCACAGCGACCTGC
  AGCACGTCATCTGCGACAAGGTGGGGCTGCAGAAGATCCCCAAGGTGTCAGAGAAGACCA
  AGCTGCTCAACCTACAGCGCAACAACTTCCCGGTGCTGGCTGCCAATTCGTTCCGGGCCA
  TGCCGAACCTCGTGTCATTGCACCTGCAGCACTGCCAGATCCGCGAGGTGGCCGCCGGTG
  CCTTCCGCGGCCTCAAGCAACTTATCTACTTGTACCTGTCCCATAACGACATCCGCGTGC
  TGCGCGCAGGTGCCTTCGACGACCTGACCGAGCTGACCTACCTCTACCTGGACCACAACA
  AGGTCACTGAGCTGCCCCGGGGGTTGCTCTCCCCGCTGGTCAACCTCTTCATCTTGCAGC
  TCAACAACAACAAGATCCGTGAGCTGCGCGCAGGCGCCTTCCAGGGAGCCAAGGACCTGC
  GCTGGCTCTACCTGTCGGAAAACGCGTTGAGCTCCCTGCAGCCCGGNGCCCTGGACGATG
  TGNGAGACCTCGCCAAATTCCACGTGGACAGGAACCAGCTGTCCAGCTACCCCTCAGCTG
  CCCTGAGCAGCTACGGNTGGGGGGAGAGCTGAAGCTGTCCCACACCCCCTGGAAAGCATC
  CCGGACATGCNCTNCAGTCCTTTGGCAGATACCTGGAGACCTCTGCTGGACACACCACCT
  GGAGAAGTCTCAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001267 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTAATACATACATACATTTAT
  ATTTATAGAAATCTCAGGAAGAAATTGCAGCATGGGAAGATGGACAGCAAAGCACCGATG
  TCCTGCTAGAACGTCCTGGCAGGTGGGGGCTGGCAGAGTTGGGGCATGGGCTTTAAAACC
  CTTTCTGGAAGCAAGGGGTGGGGCAAGGATCTGATGATTGTTTCCACGTGGCAGGGAAAG
  AGGAACAGAGGAAGGATGATCCTGGGAGGGTGGCCATCCTGATGACCAACCTGTTGTGGT
  TCTGATTGACTTGGGGGGGGGGGGGTCTCAGCAACAGCTTCTCCAAGAGGAGCTGGAGGG
  TGGGGAAGGCATGAAGGCTGGGGAGAGGCTCATCCTTCCTTCTCCCCAGGACACTGCTGG
  GTGGAGCTGGGTTGGTAGTGGAGTGCCCGAGGCCCCCTGCCAGGACGTGTCTAGGTGTAG
  GCAGCTCTGTGCATCAGCAGAGGCTGTGGGGAGAAGGTATGGGTGGAGGTGAGAAGGTCA
  GTAGTCTCTCCGGTGGAAGGCAGAGGCCAGTCACCAGGACTGGCTGGGTCAGAACCTGTT
  TAATGGCGGCCAGCTTTCTTGGACCTCTTGGTGGGGAACTTGCAGCTGCGGAAGGCGTCC
  GTGTCACGGATGTGCTGGCCCTTGAACTTGGCAGGTGAGGCACAGGTGGCATCTGGGCGG
  GAGGCCTTGGTTTCAGCCACCGCCGAAGGCCCCGGAGCTGGCAGGTACACTCCCAGGGGT
  TATTGTTAAGGGCCAGGGTCTCCAGCCTTGCCAAGGGAAATTGGAAGGTACCTGCTCAAG
  CCGTTGTCCTCCAATGACCGGTTTAAACGGGTTACCCCAAAAAGCACCTCTGAGACTTTC
  CAGGTGGGGGTAGCCCACACAAGGATCCAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001267
• Insert Size: 1920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267.1, NP_001258.1
• RefSeq Size: 1789 bp
• RefSeq ORF: 1080 bp
• Locus ID: 1101
• Cytogenetics: 17q21.33
• Domains: LRRNT, LRRCT, LRR, LRR_TYP, LRR_PS
• Protein Families: Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• Gene Summary: 'Chondroadherin is a cartilage matrix protein thought to mediate adhesion of isolated chondrocytes. The protein contains 11 leucine-rich repeats flanked by cysteine-rich regions. The chondroadherin messenger RNA is present in chondrocytes at all ages. [provided by RefSeq, Jul 2008]'