CHD2 (NM_001271) Human Untagged Clone

CAT#: SC119350

CHD2 (untagged)-Human chromodomain helicase DNA binding protein 2 (CHD2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHD2
• Synonyms: EEOC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001271 edited
  GGTCGATTCGAACCTTTTTTTGGGAGAAAAGCAGCTTTTAGGAGCTTTCTTTTCGTGCCT
  TGTTGGAAAGAAGCAGCCGTACTGAGAGCCCAGGTCGTTGTTTTTTCCAGCTTAGAAGCC
  ATGGCGCACCTCCATTTTTGTGCGCTCTCCTAATGAGGTTTTTTTTCTTTCGGACCTGTT
  TTAGTATTAATTATTGCTTTATTTTTTTGACCAGTTAACATATTTGAGGGTTATTTTATT
  TATTTTTCGTTTTTTAACGGAGGATTTTGCCTTTATTTTTAATTATTTGGGATCTGATAT
  TTTTCTACTAGTAGATAGGACTCTTGGTTTGGACATACTACATGGATCAGTAAATACCTG
  GGCACAGGACTTCAAAGCAAACACAGATTCCCCCTCCCCCTTAATATTTAAGAATTAAAA
  GATGATGAGAAATAAGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATC
  GAGTCACTCAGCCTCTGAAGAAGCTTCGGGTTCAGACTCAGGCAGTCAGTCGGAAAGTGA
  GCAGGGAAGTGATCCAGGAAGTGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTC
  TGAGAGTCAGTCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCC
  AGAAGCCAAAGAGAAGCCAGCCTCTAAGAACGAACGGATAGCTGATGTGAAGAAGATGTG
  GGAAGAATATCCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACCATC
  GCGATTTAATATTAAGGAAGAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAG
  AGGCCAGAGGCAGCTGAAAAAACAAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACA
  GGAACAAGGCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACC
  TGTCCCCAGAAGAACAGTGCCCAAACCTCGTGTTAAAAAGCAGCCGAAGACTCAGCGTGG
  AAAGAGAAAAAAGCAAGATTCTTCTGATGAGGATGATGATGATGACGAAGCTCCCAAAAG
  GCAGACTCGTCGAAGAGCGGCTAAAAACGTTAGTTACAAAGAAGATGATGACTTTGAGAC
  TGACTCAGATGATCTCATTGAAATGACTGGAGAAGGAGTTGATGAACAGCAAGATAATAG
  TGAAACTATTGAAAAGGTCTTAGATTCAAGACTGGGAAAGAAAGGAGCCACTGGAGCATC
  TACTACTGTATATGCGATTGAAGCTAATGGCGACCCTAGTGGTGACTTTGACACTGAAAA
  GGATGAAGGTGAAATCCAGTACCTCATCAAGTGGAAGGGTTGGTCTTACATCCACAGCAC
  ATGGGAGAGTGAAGAATCCTTACAGCAACAGAAAGTGAAGGGCCTAAAAAAACTAGAGAA
  CTTCAAGAAAAAAGAGGACGAAATCAAACAATGGTTAGGGAAAGTTTCTCCTGAAGATGT
  AGAATATTTCAATTGCCAACAGGAGCTGGCTTCAGAGTTGAATAAACAGTATCAGATAGT
  AGAAAGAGTAATAGCTGTGAAGACAAGTAAATCTACATTGGGTCAAACAGATTTTCCAGC
  TCATAGTCGGAAGCCGGCACCCTCAAATGAGCCCGAATATCTATGTAAATGGATGGGACT
  CCCCTATTCAGAGTGTAGCTGGGAAGATGAAGCCCTCATTGGAAAGAAATTCCAGAATTG
  CATTGACAGCTTCCACAGTAGGAACAACTCAAAAACCATCCCAACAAGAGAATGCAAGGC
  CCTGAAGCAGAGACCACGATTTGTAGCTTTAAAGAAACAACCTGCATATTTAGGAGGGGA
  GAATCTGGAACTTCGAGATTATCAGCTAGAAGGTCTAAACTGGCTAGCTCATTCCTGGTG
  CAAAAATAATAGTGTAATCCTTGCTGATGAAATGGGCCTAGGAAAGACCATCCAGACCAT
  ATCATTCCTCTCCTACCTGTTCCACCAACACCAGCTGTATGGCCCCTTTCTTATAGTCGT
  CCCTTTATCCACCCTCACCTCATGGCAGAGAGAGTTTGAAATCTGGGCACCAGAGATTAA
  CGTAGTGGTTTACATAGGTGACCTGATGAGCAGAAATACGATACGGGAATATGAATGGAT
  TCATTCCCAAACCAAAAGATTGAAGTTCAACGCACTTATAACAACATATGAGATCCTCTT
  GAAAGATAAGACTGTGCTGGGCAGTATTAACTGGGCCTTTCTGGGAGTGGATGAAGCCCA
  TCGGTTGAAGAATGATGACTCTTTATTGTATAAAACTCTGATTGATTTCAAGTCCAACCA
  TAGGCTCCTGATTACGGGGACCCCTCTTCAGAATTCCCTCAAAGAGCTCTGGTCCTTGCT
  GCACTTTATTATGCCGGAGAAGTTTGAATTTTGGGAAGATTTTGAAGAAGACCATGGGAA
  GGGGAGAGAGAATGGCTACCAGAGTCTTCATAAGGTGCTAGAGCCTTTCCTTCTCCGGAG
  AGTCAAAAAAGATGTGGAGAAATCCCTTCCTGCTAAAGTGGAACAGATTCTCAGGGTGGA
  GATGTCAGCCCTTCAGAAACAGTATTACAAGTGGATTCTGACCAGGAATTACAAGGCTCT
  TGCCAAAGGAACAAGAGGCAGCACATCTGGTTTTCTTAATATTGTGATGGAACTGAAAAA
  ATGTTGCAACCACTGCTATCTGATTAAACCCCCTGAAGAAAATGAAAGGGAAAATGGACA
  GGAGATTCTTCTGTCCCTCATAAGGAGCAGTGGGAAGTTGATTTTATTAGACAAACTGTT
  GACAAGACTTCGAGAAAGGGGGAATCGAGTGCTTATCTTCTCTCAGATGGTGAGAATGTT
  GGATATCCTGGCTGAATACCTAACTATTAAACACTATCCTTTCCAGCGTCTGGATGGTTC
  CATCAAGGGAGAAATCCGAAAACAGGCACTGGACCACTTCAATGCAGATGGGTCTGAGGA
  CTTCTGTTTCCTGCTCTCGACAAGGGCTGGTGGCCTGGGAATCAATTTGGCTTCAGCGGA
  CACAGTCGTCATCTTTGACTCTGACTGGAACCCCCAGAATGACTTGCAGGCACAAGCCCG
  AGCGCATAGAATTGGTCAAAAGAAGCAGGTAAATATTTACCGCTTAGTTACAAAGGGGAC
  TGTGGAGGAGGAGATCATAGAACGGGCCAAAAAGAAGATGGTATTAGATCATCTGGTGAT
  TCAGCGCATGGACACCACTGGCCGGACGATCCTGGAAAACAACTCAGGAAGGTCCAACTC
  AAATCCTTTTAATAAAGAAGAGCTGACAGCTATTTTGAAATTTGGAGCAGAGGATCTCTT
  CAAAGAACTGGAAGGGGAGGAATCAGAACCTCAGGAAATGGATATAGATGAAATTTTGCG
  GTTGGCTGAAACGAGAGAGAATGAAGTGTCAACAAGTGCAACAGATGAACTTCTATCACA
  GTTTAAGGTTGCCAACTTTGCAACAATGGAAGATGAAGAAGAGCTAGAAGAGCGTCCTCA
  CAAGGACTGGGATGAGATCATTCCAGAGGAACAAAGGAAAAAAGTAGAGGAGGAAGAGCG
  GCAGAAGGAGCTAGAAGAAATTTATATGCTGCCTCGAATTCGGAGTTCCACTAAAAAGGC
  TCAGACAAATGACAGTGACTCTGACACTGAGTCTAAGAGGCAGGCCCAGAGATCCTCTGC
  TTCTGAGAGTGAAACGGAAGACTCTGATGATGACAAGAAGCCAAAGCGCAGAGGGCGTCC
  GAGGAGTGTGCGGAAGGACCTCGTGGAGGGATTTACTGATGCAGAGATCCGAAGGTTCAT
  CAAGGCTTATAAGAAGTTTGGTCTCCCTCTTGAACGGCTGGAGTGCATAGCACGTGATGC
  TGAGCTGGTAGATAAGTCGGTGGCAGATCTGAAGCGCCTGGGTGAACTGATCCACAACAG
  CTGTGTGTCAGCAATGCAGGAATACGAAGAGCAGCTGAAAGAAAATGCCAGCGAGGGAAA
  AGGACCAGGGAAAAGGAGAGGTCCAACAATCAAGATATCCGGAGTTCAGGTTAATGTGAA
  ATCCATTATCCAACATGAAGAGGAGTTTGAGATGCTGCATAAATCTATCCCTGTGGACCC
  TGAAGAAAAAAAAAAATACTGCTTAACCTGTCGTGTCAAAGCTGCACATTTTGATGTAGA
  GTGGGGGGTGGAAGATGATTCTCGCCTGTTGCTGGGGATTTATGAACATGGCTATGGAAA
  CTGGGAGTTAATTAAAACAGACCCAGAGCTTAAATTAACTGACAAAATTCTGCCGGTGGA
  GACAGATAAAAAGCCTCAGGGGAAGCAGCTACAGACCCGAGCGGATTACTTGTTGAAGCT
  GCTCAGAAAGGGTCTGGAGAAGAAGGGGGCTGTGACAGGTGGGGAAGAGGCCAAATTAAA
  GAAGCGGAAGCCTCGGGTAAAGAAGGAAAACAAAGTGCCCAGGCTGAAAGAGGAGCATGG
  AATTGAGCTTTCATCTCCTAGGCATTCAGATAATCCATCAGAAGAGGGAGAAGTGAAAGA
  TGATGGCTTGGAAAAAAGTCCAATGAAAAAAAAACAGAAGAAGAAAGAGAACAAGGAGAA
  CAAGGAGAAACAAATGAGTTCTAGGAAAGACAAAGAAGGGGACAAGGAAAGAAAGAAGTC
  AAAAGATAAGAAAGAGAAGCCTAAAAGTGGTGATGCCAAATCTTCGAGTAAATCAAAGCG
  ATCTCAGGGTCCTGTCCATATTACAGCAGGAAGTGAACCTGTCCCCATTGGAGAGGATGA
  GGATGATGATCTGGACCAGGAGACATTCAGCATATGTAAGGAGAGGATGAGGCCCGTGAA
  AAAGGCACTGAAACAGCTCGACAAACCTGACAAGGGGCTCAACGTGCAAGAACAGCTGGA
  ACACACCCGGAACTGCCTGCTGAAAATCGGAGACCGGATAGCCGAGTGCCTTAAAGCCTA
  CTCAGATCAGGAGCACATCAAACTCTGGAGGAGGAACCTATGGATTTTTGTTTCCAAGTT
  TACAGAATTTGATGCTCGAAAACTGCATAAGTTATACAAGATGGCTCATAAGAAAAGGTC
  TCAAGAAGAAGAGGAGCAAAAGAAGAAAGACGACGTGACTGGGGGTAAGAAACCATTTCG
  TCCAGAGGCCTCAGGCTCCAGCCGGGACTCTCTGATATCTCAGTCCCATACCTCACACAA
  CCTTCACCCTCAGAAGCCTCATTTGCCTGCCTCCCATGGCCCACAGATGCATGGACACCC
  AAGAGATAACTACAATCACCCCAACAAGAGACACTTCAGTAATGCAGATCGAGGAGACTG
  GCAGAGGGAAAGAAAGTTCAACTATGGTGGTGGCAACAACAATCCACCATGGGGAAGCGA
  CAGGCACCATCAGTATGAGCAGCACTGGTACAAGGACCACCATTATGGGGACCGGCGACA
  TATGGATGCCCACCGTTCCGGAAGCTATCGACCCAACAACATGTCCAGAAAGAGGCCTTA
  TGACCAGTACAGCAGTGACCGAGACCACCGGGGACACAGAGATTATTATGACAGGCACCA
  TCATGACTCCAAGCGGAGGAGATCCGATGAATTTAGGCCTCAAAATTACCACCAGCAGGA
  TTTCCGACGAATGTCTGATCACCGCCCCGCTATGGGCTACCATGGCCAGGGACCCTCAGA
  CCATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCCATT
  GCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCACGATTCCAA
  GTCACCCCTGGATCATAGGTCTCCTTTGGAGAGATCACTAGAACAGAAAAACAACCCAGA
  TTATAACTGGAATGTTCGGAAAACATAAAGGACAGCTCGTAAAGGAGAGAGTAAGAGTCA
  CCAAACACGTGGATATTTTTGGTCTGATCCTACAGTAGCCGGTTATCTAGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001271
• Insert Size: 6100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: It is not a varient. This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271.1, NP_001262.1
• RefSeq Size: 7764 bp
• RefSeq ORF: 5220 bp
• Locus ID: 1106
• Cytogenetics: 15q26.1
• Domains: SNF2_N, CHROMO, DEAD, helicase_C
• Protein Families: Transcription Factors
• Gene Summary: 'The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).