RPL22 (NM_000983) Human Untagged Clone

CAT#: SC119490

RPL22 (untagged)-Human ribosomal protein L22 (RPL22)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL22
• Synonyms: EAP; HBP15; HBP15/L22; L22
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000983 edited
  ATGGCTCCTGTGAAAAAGCTTGTGGTGAAGGGGGGCAAAAAAAAGAAGCAAGTTCTGAAG
  TTCACTCTTGATTGCACCCACCCTGTAGAAGATGGAATCATGGATGCTGCCAATTTTGAG
  CAGTTTTTGCAAGAAAGGATCAAAGTGAACGGAAAAGCTGGGAACCTTGGTGGAGGGGTG
  GTGACCATCGAAAGGAGCAAGAGCAAGATCACCGTGACATCCGAGGTGCCTTTCTCCAAA
  AGGTATTTGAAATATCTCACCAAAAAATATTTGAAGAAGAATAATCTACGTGACTGGTTG
  CGCGTAGTTGCTAACAGCAAAGAGAGTTACGAATTACGTTACTTCCAGATTAACCAGGAC
  GAAGAAGAGGAGGAAGACGAGGATTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000983 unedited
  GTTACCATTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGCACGAGGTGCCGCCAT
  GGCTCCTGTGAAAAACTTGTGGTGAAGGGGGGCAAAAAAAAGAAGCAAGTTCTGAAGTTC
  ACTCTTGATTGCACCCACCCTGTAGAAGATGGAATCATGGATGCTGCCAATTTTGAGCAG
  TTTTTGCAAGAAAGGATCAAAGTGAACGGAAAAGCTGGGAACCTTGGTGGAGGGGTGGTG
  ACCATCGAAAGGAGCAAGAGCAAGATCACCGTGACATCCGAGGTGCCTTTCTCCAAAAGG
  TATTTGAAATATCTCACCAAAAAATATTTGAAGAAGAATAATCTACGTGACTGGTTGCGC
  GTAGTTGCTAACAGCAAAGAGAGTTACGAATTACGTTACTTCCAGATTAACCAGGACGAA
  GAAGAGGAGGAAGACGAGGATTAAATTTCATTTATCTGGAAAATTTTGTATGAGTTCTTG
  AATAAAACTTGGGAACCAAAATGGTGGTTTATCCTTGTATCTCTGCAGTGTGGATTGAAC
  AGAAAATTGGAAATCATAGTCAAAGGGCTTCCCTTGGTTCGCCACTCATTTATTTGTAAC
  TTGACTTCTTTTTTTTTCTGCTTAAAAATTTCAATTCTCGTGGGTATACCAGAGTAGAAG
  GAGAGGGTGACTTTACCGAACTGACAGCCATTGGGGAGGCANATGCGGGTGTGGGAGTGT
  GGGCCTGAAGTANTGACTGTTTGATTTAAAAAGTGTGACTGTCACGTGTATCTGTTGCTT
  TTCTCAATGATTCAGGGATACAAATGGGGCTTCTCTCATTCATTAAAAGAAAACCCGACA
  TCTTTTCTAAGATCTCTTGGGGAAAATGACTGTCAATAAATGCCGGGTTTTGGGGCCATC
  CGCCTACTTTCATTTTTGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000983 unedited
  TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAGCCAGGTGTT
  AGCCACTTTAATAGAAATATGATCAAAACTCGATTACAAGAGTTCAAAAAGACATAGAAA
  ACCAGTGAGTTTCAATTTTATTACAAGTTTTCAAATCTGGGACTAGTTTCTTTTTTTCTT
  TTAACTGAAATGCCAACTTCAGCCCAGGGTTTTTTCACAACCAAACTAAAAATGACTTAC
  TACATGGGAACATCAATGCAACAAGTAGAATTTGTAAACTCAAGCCACAAACTTAGTTAA
  TAATCATGGTTAAGGGACATTGCCAAAGAGCAACTGATGCCTCAGTGAAGTTTGAAAGAA
  ACTCTGCTTTCTGTGACGGCAGAGAAGAAATATGCAAGCAATTCTGCTTCAAAGAAATTT
  GCATAGAAATGGAAAAATGCCAGAGCCTTTAACACAAGTGAAATTGCAAAGCCTCAACAC
  GTTCAACTCAATCCACAGAGCACCAAATGTTTAATGGGAGCCAAGGTAGGACTGAGCATT
  GAACTTCCAGCTATGCAACTCGCAGGGCACAATTTCAAGTGTGGAAACCATCTGTAGGCA
  AGCTCTTTTAAAAACATGAATTTTAGACACCGTANATTCTAATGCAGACACTTTTGCATT
  ACTGTTTGAATTTCAGAAGGGCACCACAAGGCACCAGTCTTTCAAGTCACTCACAGNCAC
  ANNTGCATTNTTTTCCCAGCCTTGGTAGCCCCTTTCAGTTGTCTAGCCTCTGCACTGTGG
  CACACCACTGACATTAAATCCAGTCATGGCCTAAGTGGCATGAGGCAGCTATTTCCAGGG
  CACCTNCCACCTCCTTGGCATGGGAGGGCTTCCAGCACTNCTNCTTCNGTTCCCTCTTTC
  AGACCTNCAAGCTCTGNCATCAGGGCCCCCATGCTGGCCCCACCGGAGACCCTCGGAACC
  TATTCTACGCTCCTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000983
• Insert Size: 2170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000983.3, NP_000974.1
• RefSeq Size: 2099 bp
• RefSeq ORF: 387 bp
• Locus ID: 6146
• Cytogenetics: 1p36.31
• Domains: Ribosomal_L22e
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]'