MGP (NM_000900) Human Untagged Clone

CAT#: SC119598

MGP (untagged)-Human matrix Gla protein (MGP), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGP
• Synonyms: GIG36; MGLAP; NTI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119598 sequence for NM_000900 edited (data generated by NextGen Sequencing)
  ATGAAGAGCCTGATCCTTCTTGCCATCCTGGCCGCCTTAGCGGTAGTAACTTTGTGTTAT
  GAATCACATGAAAGCATGGAATCTTATGAACTTAATCCCTTCATTAACAGGAGAAATGCA
  AATACCTTCATATCCCCTCAGCAGAGATGGAGAGCTAAAGTCCAAGAGAGGATCCGAGAA
  CGCTCTAAGCCTGTCCACGAGCTCAATAGGGAAGCCTGTGATGACTACAGACTTTGCGAA
  CGCTACGCCATGGTTTATGGATACAATGCTGCCTATAATCGCTACTTCAGGAAGCGCCGA
  GGGACCAAATGA
  
  Clone variation with respect to NM_000900.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000900 unedited
  GCACGAGGATCCCGTAGGAGCCTCTCTCCCTACTGCTGCTACACAAGACCCTGAGACTGA
  CCTGCAGGACGAAACCATGAAGAGCCTGATCCTTCTTGCCATCCTGGCCGCCTTAGCGGT
  AGTAACTTTGTGTTATGAATCACATGAAAGCATGGAATCTTATGAACTTAATCCCTTCAT
  TAACAGGAGAAATGCAAATACCTTCATATCCCCTCAGCAGAGATGGAGAGCTAAAGTCCA
  AGAGAGGATCCGAGAACGCTCTAAGCCTGTCCACGAGCTCAATAGGGAAGCCTGTGATGA
  CTACAGACTTTGCGAACGCTACGCCATGGTTTATGGATACAATGCTGCCTATAATCGCTA
  CTTCAGGAAGCGCCGAGGGACCAAATGAGACTGAGGGAAGAAAAAAAATCTCTTTTTTTC
  TGGAGGCTGGCACCTGATTTTGTATCCCCCTGTAGCAGCATTACTGAAATACATAGGCTT
  ATATACAATGCTTCTTTCCTGTATATTCTCTTGTCTGGCTGCACCCCTTTTTCCCGCCCC
  CAGATTGATAAGTAATGAAAGTGCACTGCAGTGAGGGTCAAAGGAGAGTCAACATATGTG
  ATTGTTCCATAATAAACTTCTGGTGTGATACTTTCAAAAAAAAAAAAAAAAAACTCGACT
  CTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGA
  CCCCTCCCCAGTGCCTCTCCTGGGCCCTGGAGTTGCCACTCCAGTGCCCACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000900 unedited
  TTATCTTGNACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAAGTATCA
  CACCAGAAGTTTATTATGGAACAATCACATATGTTGACTCTCCTTTGACCCTCACTGCAG
  TGCACTTTCATTACTTATCAATCTGGGGGCGGGAAAAAGGGGTGCAGCCAGACAAGAGAA
  TATACAGGAAAGAAGCATTGTATATAAGCCTATGTATTTCAGTAATGCTGCTACAGGGGG
  ATACAAAATCAGGTGCCAGCCTCCAGAAAAAAAGAGATTTTTTTTCTTCCCTCAGTCTCA
  TTTGGTCCCTCGGCGCTTCCTGAAGTAGCGATTATAGGCAGCATTGTATCCATAAACCAT
  GGCGTAGCGTTCGCAAAGTCTGTAGTCATCACAGGCTTCCCTATTGAGCTCGTGGACAGG
  CTTAGAGCGTTCTCGGATCCTCTCTTGGACTTTAGCTCTCCATCTCTGCTGAGGGGATAT
  GAAGGTATTTGCATTTCTCCTGTTAATGAAGGGATTAAGTTCATAAGATTCCATGCTTTC
  ATGTGATTCATAACACAAAGTTACTACCGCTAAGGCGGCCAGGATGGCAAGAAGGATCAG
  GCTCTTCATGGTTTCGTCCTGCAGGTCAGTCTCAGGGTCTTGTGTAGCAGCAGTAGGGAG
  AGAGGCTCCTACGGGATCCTCGTGCCGAATTCGCCGCCGNCCTATAGTGAGTCGTATTAC
  AAAATTCTGACGGTTCCACTAACGAGCTCTGGCTATATAGACCTCCCACCGTACACGCCT
  ACCGCCCATTTGCGTCAACGGGGGCGGGGTATTACGACATTTTGGAAAGTCCCGTTGATT
  TTGTGCCCAAACAACTCCAATTGACGGTCATGGGGGGGAGACTTGGAAACCCCGGGAGTC
  AAACCGCTATCCGGCCCATGGGGTACTGCCAAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000900
• ORF Size: 312 bp
• Insert Size: 700
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_000900.2, NP_000891.2
• RefSeq Size: 661
• RefSeq ORF: 312
• Locus ID: 4256
• Domains: GLA
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes a member of the osteocalcin/matrix Gla family of proteins. The encoded vitamin K-dependent protein is secreted by chondrocytes and vascular smooth muscle cells, and functions as a physiological inhibitor of ectopic tissue calcification. Carboxylation status of the encoded protein is associated with calcification of the vasculature in human patients with cardiovascular disease and calcification of the synovial membranes in osteoarthritis patients. Mutations in this gene cause Keutel syndrome in human patients, which is characterized by abnormal cartilage calcification, peripheral pulmonary stenosis and facial hypoplasia. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (2) represents the shorter transcript and encodes the shorter isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.