GART (NM_000819) Human Untagged Clone

CAT#: SC119632

GART (untagged)-Human phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (GART), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GART
• Synonyms: AIRS; GARS; GARTF; PAIS; PGFT; PRGS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000819, the custom clone sequence may differ by one or more nucleotides
  
  
  CCGCGAATTCGGCACGAGGCGGGAACCAGGTGGCCACCCGGTGTCGGTTTCATTTTCCTTTGGAATTTCT
  GCTTTACAGACAGAACAATGGCAGCCCGAGTACTTATAATTGGCAGTGGAGGAAGGGAACATACGCTGGC
  CTGGAAACTTGCACAGTCTCATCATGTCAAACAAGTGTTGGTTGCCCCAGGAAACGCAGGCACTGCCTGC
  TCTGAAAAGATTTCAAATACCGCCATCTCAATCAGTGACCACACTGCCCTTGCTCAATTCTGCAAAGAGA
  AGAAAATTGAATTTGTAGTTGTTGGACCAGAAGCACCTCTGGCTGCTGGGATTGTTGGGAACCTGAGGTC
  TGCAGGAGTGCAATGCTTTGGCCCAACAGCAGAAGCGGCTCAGTTAGAGTCCAGCAAAAGGTTTGCCAAA
  GAGTTTATGGACAGACATGGAATCCCAACCGCACAATGGAAGGCTTTCACCAAACCTGAAGAAGCCTGCA
  GCTTCATTTTGAGTGCAGACTTCCCTGCTTTGGTTGTGAAGGCCAGTGGTCTTGCAGCTGGAAAAGGGGT
  GATTGTTGCAAAGAGCAAAGAAGAGGCCTGCAAAGCTGTACAAGAGATCATGCAGGAGAAAGCCTTTGGG
  GCAGCTGGAGAAACAATTGTCATTGAAGAACTTCTTGACGGAGAAGAGGTGTCGTGTCTGTGTTTCACTG
  ATGGCAAGACTGTGGCCCCCATGCCCCCAGCACAGGACCATAAGCGATTACTGGAGGGAGATGGTGGCCC
  TAACACAGGGGGAATGGGAGCCTATTGTCCAGCCCCTCAGGTTTCTAATGATCTATTACTAAAAATTAAA
  GATACTGTTCTTCAGAGGACAGTGGATGGCATGCAGCAAGAGGGTACTCCATATACAGGTATTCTCTATG
  CTGGAATAATGCTGACCAAGAATGGCCCAAAAGTTCTAGAGTTTAATTGCCGTTTTGGTGATCCAGAGTG
  CCAAGTAATCCTCCCACTTCTTAAAAGTGATCTTTATGAAGTGATTCAGTCCACCTTAGATGGACTGCTC
  TGCACATCTCTGCCTGTTTGGCTAGAAAACCACACCGCCCTAACTGTTGTCATGGCAAGTAAAGGTTATC
  CTGGAGACTACACCAAGGGTGTAGAGATAACAGGGTTTCCTGAGGCTCAAGCTCTAGGACTGGAGGTGTT
  CCATGCAGGCACTGCCCTCAAAAATGGCAAAGTAGTAACTCATGGGGGTAGAGTTCTTGCAGTCACAGCC
  ATCCGGGAAAATCTCATATCAGCCCTTGAGGAAGCCAAGAAAGGACTAGCTGCTATAAAGTTTGAGGGAG
  CAATTTATAGGAAAGACATCGGCTTTCGTGCCATAGCTTTCCTCCAGCAGCCCAGGAGTTTGACTTACAA
  GGAATCTGGAGTAGATATCGCAGCTGGAAATATGCTGGTCAAGAAAATTCAGCCTTTAGCAAAAGCCACT
  TCCAGATCAGGCTGTAAAGTTGATCTTGGAGGTTTTGCTGGTCTTTTTGATTTAAAAGCAGCTGGTTTCA
  AAGATCCCCTTCTGGCCTCTGGAACAGATGGCGTTGGAACTAAACTAAAGATTGCCCAGCTATGCAATAA
  ACATGATACCATTGGTCAAGATTTGGTAGCAATGTGTGTTAATGATATTCTGGCACAAGGAGCAGAGCCC
  CTCTTCTTCCTTGATTACTTTTCCTGTGGAAAACTTGACCTCAGTGTAACTGAAGCTGTTGTTGCTGGAA
  TTGCTAAAGCTTGTGGAAAAGCTGGATGTGCTCTCCTTGGAGGTGAAACAGCAGAAATGCCTGACATGTA
  TCCCCCTGGAGAGTATGACCTAGCTGGGTTTGCCGTTGGTGCCATGGAGCGAGATCAGAAACTCCCTCAC
  CTGGAAAGAATCACTGAGGGTGATGTTGTTGTTGGAATAGCTTCATCTGGTCTTCATAGCAATGGATTTA
  GCCTTGTGAGGAAAATCGTGGCAAAATCTTCCCTCCAGTACTCCTCTCCAGCACCTGATGGTTGTGGTGA
  CCAGACTTTAGGGGACTTACTTCTCACGCCTACCAGAATCTACAGCCATTCACTGTTACCTGTCCTACGT
  TCAGGACATGTCAAAGCCTTTGCCCATATTACTGGTGGAGGATTACTAGAGAACATCCCCAGAGTCCTCC
  CTGAGAAACTTGGGGTAGATTTAGATGCCCAGACCTGGAGGATCCCCAGGGTCTTCTCATGGTTGCAGCA
  GGAAGGACACCTCTCTGAGGAAGAGATGGCCAGAACATTTAACTGTGGGGTTGGCGCTGTCCTTGTGGTA
  TCAAAGGAGCAGACAGAGCAGATTCTGAGGGATATCCAGCAGCACAAGGAAGAAGCCTGGGTGATTGGCA
  GTGTGGTTGCACGAGCTGAAGGTTCCCCACGTGTGAAAGTCAAGAATCTGATTGAAAGCATGCAAATAAA
  TGGGTCAGTGTTGAAGAATGGCTCCCTGACAAATCATTTCTCTTTTGAAAAAAAAAAGGCCAGAGTGGCT
  GTCTTAATATCTGGAACAGGAAAAATGCTCAATATCCACCCATCCTTGCTCCCTTCTTTTAAGGGTTCAA
  ATGCCCATGAGCAAGCCCTGGAAACCGGAGTCACAGTTACTGGGTGCACTGTACACTTTGTAGCTGAAGA
  TGTGGATGCTGGACAGATTATTTTGCAAGAAGCTGTTCCCGTGAAGAGGGGTGATACTGTCGCAACTCTT
  TCTGAAAGAGTAAAATTAGCAGAACATAAAATATTTCCTGCAGCCCTTCAGCTGGTGGCCAGTGGAACTG
  TACAGCTTGGAGAAAATGGCAAGATCTGTTGGGTTAAAGAGGAATGAAGCCTTTTAATTCAGAAATGGGG
  CCAGTTTAGAAAGAATTATTTGCTGTTTGCATGGTGGTTTTTTATCATGGACTTGGCCCAAAAGAAAAAC
  TGCTAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000819 unedited
  NNGGTTCAGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGGAAC
  CAGGTGGCCACCCGGTGTCGGTTTCATTTTCCTTTGGAATTTCTGCTTTACAGACAGAAC
  AATGGCAGCCCGAGTACTTATAATTGGCAGTGGAGGAAGGGAACATACGCTGGCCTGGAA
  ACTTGCACAGTCTCATCATGTCAAACAAGTGTTGGTTGCCCCAGGAAACGCAGGCACTGC
  CTGCTCTGAAAAGATTTCAAATACCGCCATCTCAATCAGTGACCACACTGCCCTTGCTCA
  ATTCTGCAAAGAGAAGAAAATTGAATTTGTAGTTGTTGGACCAGAAGCACCTCTGGCTGC
  TGGGATTGTTGGGAACCTGAGGTCTGCAGGAGTGCAATGCTTTGGCCCAACAGCAGAAGC
  GGCTCAGTTAGAGTCCAGCAAAAGGTTTGCCAAAGAGTTTATGGACAGACATGGAATCCC
  AACCGCACAATGGAAGGCTTTCACCAAACCTGAAGAAGCCTGCAGCTTCATTTTGAGTGC
  AGACTTCCCTGCTTTGGTTGTGAAGGCCAGTGGTCTTGCAGCTGGAAAAGGGGTGATTGT
  TGCAAAGAGCAAAGAAGAGGCCTGCAAAGCTGTACAAGAGATCATGCAGGAGAAAGCCTT
  TGGGGCAGCTGGAGAAACAATTGTCATTGAAGAACTTCTTGACGGAGAAGAGGTGTCGTG
  TCTGTGTTTCACTGATGGCAAGACTGTGGCCCCCATGCCCNCAGCACAGGACCATTAGCG
  ATTACTGGAGGGAGATGGTGGGCCCTACACAGGNGGAATGGGAGCCTATTGTNCAGCCCT
  CAGGTTCTAATGATCTATTACTAANAAATAAGATACTGNTCTTCANNAAGACAGTGGATG
  GCATGCAGCNAGAGGGGTACTCTNATACAGGGTATTCTCTATGCTGGAATAATGCTGACC
  AGAATGGNCCAAAGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000819 unedited
  CTANNGATCGATTTTTTTTTTTTGTTTTTAGTGAGTCTCTATTTATTAAAAAAAAGATGA
  AGTAAGGGTGAGGTCTTTTTTGTCTTTTAGCAGTTTTTCTTTTGGGCCAAGTCCATGATA
  AAAAACCACCATGCAAACAGCAAATAATTCTTTCTAAACTGGCCCCATTTCTGAATTAAA
  AGGCTTCATTCCTCTTTAACCCAACAGATCTTGCCATTTTCTCCAAGCTGTACAGTTCCA
  CTGGCCACCAGCTGAAGGGCTGCAGGAAATATTTTATGTTCTGCTAATTTTACTCTTTCA
  GAAAGAGTTGCGACAGTATCACCCCTCTTCACGGGAACAGCTTCTTGCAAAATAATCTGT
  CCAGCATCCACATCTTCAGCTACAAAGTGTACAGTGCACCCAGTAACTGTGACTCCGGTT
  TCCAGGGCTTGCTCATGGGCATTTGAACCCTTAAAAGAAGGGAGCAAGGATGGGTGGATA
  TTGAGCATTTTTCCTGTTCCAGATATTAAGACAGCCACTCTGGCCTTTTTTTTTTCAAAA
  GAGAAATGATTTGTCAGGGAGCCATTCTTCAACACTGACCCATTTATTTGCATGCTTTCA
  ATCAGATTCTTGACTTTCACACGTGGGGAACCTTCAGCTCGTGCAACCACACTGCCAATC
  ACCCAGGCTTCTTCCTTGTGCTGCTGGATATCCCTCAGAATCTGCTCTGTCTGCTCCTTT
  GATACCACAAGGACAGCGCCAACCCCACAGTTAAATGTTCTGGCCATCTCTTCCTCAGAA
  AGGTGTCCTTCCTGCTGCAACCATGAGAAGACCCTGGGGATCCTCCAGGTCCTGGGCATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000819
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000819.3, NP_000810.1
• RefSeq Size: 3376 bp
• RefSeq ORF: 3033 bp
• Locus ID: 2618
• Cytogenetics: 21q22.11
• Domains: GARS, AIRS, formyl_transf
• Protein Pathways: Metabolic pathways, One carbon pool by folate, Purine metabolism
• Gene Summary: 'The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Variants 1, 3, and 4 encode the same protein.