Metabotropic Glutamate Receptor 5 (GRM5) (NM_000842) Human Untagged Clone

CAT#: SC119648

GRM5 (untagged)-Human glutamate receptor, metabotropic 5 (GRM5), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRM5
• Synonyms: GPRC1E; mGlu5; MGLUR5; PPP1R86
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000842 edited
  TCGGCACGAGGGGCACGTGCTCCCGGCGCTGGCGCGAGAGAGCGAGCGCCACCGCCGCGG
  GCCCCGCAGCCGTTCTGCCTGCTGTCACCGCTGCCTCCATCGCCGACACTAGCGCTCCAG
  CTGCAGCCAAGGCCGCTACGAGAGCGCAGGAAGCCCTCGAGGAGCGGCTGCCTGGGGGCG
  CAAGGCTCAGGGCGCGCACCTGGTTTAGAAGATCATGACCACATGGATCATCTAACTAAA
  TGGTACATGGGGACAAAATGGTCCTTTAGAAAATACATCTGAATTGCTGGCTAATTTCTT
  GATTTGCGACTCAACGTAGGACATCGCTTGTTCGTAGCTATCAGAACCCTCCTGAATTTT
  CCCCACCATGCTATCTTTATTGGCTTGAACTCCTTTCCTAAAATGGTCCTTCTGTTGATC
  CTGTCAGTCTTACTTTTGAAAGAAGATGTCCGTGGGAGTGCACAGTCCAGTGAGAGGAGG
  GTGGTGGCTCACATGCCGGGTGACATCATTATTGGAGCTCTCTTTTCTGTTCATCACCAG
  CCTACTGTGGACAAAGTTCATGAGAGGAAGTGTGGGGCGGTCCGTGAACAGTATGGCATT
  CAGAGAGTGGAGGCCATGCTGCATACCCTGGAAAGGATCAATTCAGACCCCACACTCTTG
  CCCAACATCACACTGGGCTGTGAGATAAGGGACTCCTGCTGGCATTCGGCTGTGGCCCTA
  GAGCAGAGCATTGAGTTCATAAGAGATTCCCTCATTTCTTCAGAAGAGGAAGAAGGCTTG
  GTACGCTGTGTGGATGGCTCCTCCTCTTCCTTCCGCTCCAAGAAGCCCATAGTAGGGGTC
  ATTGGGCCTGGCTCCAGTTCTGTAGCCATTCAGGTCCAGAATTTGCTCCAGCTTTTCAAC
  ATACCTCAGATTGCTTACTCAGCAACCAGCATGGATCTGAGTGACAAGACTCTGTTCAAA
  TATTTCATGAGGGTTGTGCCTTCAGATGCTCAGCAGGCAAGGGCCATGGTGGACATAGTG
  AAGAGGTACAACTGGACCTATGTATCAGCCGTGCACACAGAAGGCAACTATGGAGAAAGT
  GGGATGGAAGCCTTCAAAGATATGTCAGCGAAGGAAGGGATTTGCATCGCCCACTCTTAC
  AAAATCTACAGTAATGCAGGGGAGCAGAGCTTTGATAAGCTGCTGAAGAAGCTCACAAGT
  CACTTGCCCAAGGCCCGGGTGGTGGCCTGCTTCTGTGAGGGCATGACGGTGAGAGGTCTG
  CTGATGGCCATGAGGCGCCTGGGTCTAGCGGGAGAATTTCTGCTTCTGGGCAGTGATGGC
  TGGGCTGACAGGTATGATGTGACAGATGGATATCAGCGAGAAGCTGTTGGTGGCATCACA
  ATCAAGCTCCAATCTCCCGATGTCAAGTGGTTTGATGATTATTATCTGAAGCTCCGGCCA
  GAAACAAACCACCGAAACCCTTGGTTTCAAGAATTTTGGCAGCATCGTTTTCAGTGCCGA
  CTGGAAGGGTTTCCACAGGAGAACAGCAAATACAACAAGACTTGCAATAGTTCTCTGACT
  CTGAAAACACATCATGTTCAGGATTCCAAAATGGGATTTGTGATCAACGCCATCTATTCG
  ATGGCCTATGGGCTCCACAACATGCAGATGTCCCTCTGCCCAGGCTATGCAGGACTCTGT
  GATGCCATGAAGCCAATTGATGGACGGAAACTTTTGGAGTCCCTGATGAAAACCAATTTT
  ACTGGGGTTTCTGGAGATACGATCCTATTCGATGAGAATGGAGACTCTCCAGGAAGGTAT
  GAAATAATGAATTTCAAGGAAATGGGAAAAGATTACTTTGATTATATCAACGTTGGAAGT
  TGGGACAATGGAGAATTAAAAATGGATGATGATGAAGTATGGTCCAAGAAAAGCAACATC
  ATCAGATCTGTGTGCAGTGAACCATGTGAGAAAGGCCAGATCAAGGTGATCCGAAAGGGA
  GAAGTCAGCTGTTGTTGGACCTGTACACCTTGTAAGGAGAATGAGTATGTCTTTGATGAG
  TACACATGCAAGGCATGCCAACTGGGGTCTTGGCCCACTGATGATCTCACAGGTTGTGAC
  TTGATCCCAGTACAGTATCTTCGATGGGGTGACCCTGAACCCATTGCAGCTGTGGTGTTT
  GCCTGCCTTGGCCTCCTGGCCACCCTGTTTGTTACTGTAGTCTTCATCATTTACCGTGAT
  ACACCAGTAGTCAAGTCCTCAAGCAGGGAACTCTGCTACATTATCCTTGCTGGCATCTGC
  CTGGGCTACTTATGTACCTTCTGCCTCATTGCGAAGCCCAAACAGATTTACTGCTACCTT
  CAGAGAATTGGCATTGGTCTCTCCCCAGCCATGAGCTACTCAGCCCTTGTAACAAAGACC
  AACCGTATTGCAAGGATCCTGGCTGGCAGCAAGAAGAAGATCTGTACCAAAAAGCCCAGA
  TTCATGAGTGCCTGTGCCCAGCTAGTGATTGCTTTCATTCTCATATGCATCCAGTTGGGC
  ATCATCGTTGCCCTCTTTATAATGGAGCCTCCTGACATAATGCATGACTACCCAAGCATT
  CGAGAAGTCTACCTGATCTGTAACACCACCAACCTAGGAGTTGTCACTCCACTTGGATAC
  AATGGATTGTTGATTTTGAGCTGCACCTTCTATGCGTTCAAGACCAGAAATGTTCCAGCT
  AACTTCAACGAGGCCAAGTATATCGCCTTCACAATGTACACGACCTGCATTATATGGCTA
  GCTTTTGTGCCAATCTACTTTGGCAGCAACTACAAAATCATCACCATGTGTTTCTCGGTC
  AGCCTCAGTGCCACAGTGGCCCTAGGCTGCATGTTTGTGCCGAAGGTGTACATCATCCTG
  GCCAAACCAGAGAGAAACGTGCGCAGCGCCTTCACCACATCTACCGTGGTGCGCATGCAT
  GTAGGGGATGGCAAGTCATCCTCCGCAGCCAGCAGATCCAGCAGCCTAGTCAACCTGTGG
  AAGAGAAGGGGCTCCTCTGGGGAAACCTTAAGTTCCAATGGAAAATCCGTCACGTGGGCC
  CAGAATGAGAAGAGCAGCCGGGGGCAGCACCTGTGGCAGCGCCTGTCCATCCACATCAAC
  AAGAAAGAAAACCCCAACCAAACGGCCGTCATCAAGCCCTTCCCCAAGAGCACGGAGAGC
  CGTGGCCTGGGCGCTGGCGCTGGCGCAGGCGGGAGCGCTGGGGGCGTGGGGGCCACGGGC
  GGTGCGGGCTGCGCAGGCGCCGGCCCAGGCGGGCCCGAGTCCCCAGACGCCGGCCCCAAG
  GCGCTGTATGATGTGGCCGAGGCTGAGGAGCACTTCCCGGCGCCCGCGCGGCCGCGCTCA
  CCGTCGCCCATCAGCACGCTGAGCCACCGCGCGGGCTCGGCCAGCCGCACGGACGACGAT
  GTGCCGTCGCTGCACTCGGAGCCTGTGGCGCGCAGCAGCTCCTCGCAGGGCTCCCTCATG
  GAGCAGATCAGCAGTGTGGTCACCCGCTTCACGGCCAACATCAGCGAGCTCAACTCCATG
  ATGCTGTCCACCGCGGCCCCCAGCCCCGGCGTCGGCGCCCCGCTCTGCTCGTCCTACCTG
  ATCCCCAAAGAGATCCAGTTGCCCACGACCATGACGACCTTTGCCGAAATCCAGCCTCTG
  CCGGCCATCGAAGTCACGGGCGGCGCGCAGCCCGCGGCAGGGGCGCAGGCGGCTGGGGAC
  GCGGCCCGGGAGAGCCCCGCGGCCGGTCCCGAGGCTGCGGCCGCCAAGCCAGACCTGGAG
  GAGCTGGTGGCTCTCACCCCGCCGTCCCCCTTCAGAGACTCGGTGGACTCGGGGAGCACA
  ACCCCCAACTCGCCAGTGTCCGAGTCGGCCCTCTGTATCCCGTCGTCTCCCAAATATGAC
  ACTCTTATCATAAGAGATTACACTCAGAGCTCCTCGTCGTTGTGAATGTCCCTGGAAAGC
  ACGCCGGCCTGCGCGTGCGGAGCGGAGCCCCCCGTGTTCACACACACACAATGGCAAGCA
  TAGTCGCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000842 unedited
  NGGTTAGAATTGTAAACGACTCATATAGGCGGCCGCGAAATCGGCACGAGGGGCACGTGC
  TCCCGGCGCTGGCGCGAGAGACGAGCGCCACCGCCGCGGGCCCCGCAGCCGTTCTGCCTG
  CTGTCACCGCTGCCTCCATCGCCGACACTAGCGCTCCAGCTGCAGCCAAGGCCGCTACGA
  GAGCGCAGGAAGCCCTCGAGGAGCGGCTGCCTGGGGGCGCAAGGCTCAGGGCGCGCACCT
  GGTTTAGAAGATCATGACCACATGGATCATCTAACTAAATGGTACATGGGGACAAAATGG
  TCCTTTAGAAAATACATCTGAATTGCTGGCTAATTTCTTGATTTGCGACTCAACGTAGGA
  CATCGCTTGTTCGTAGCTATCAGAACCCTCCTGAATTTTCCCCACCATGCTATCTTTATT
  GGCTTGAACTCCTTTCCTAAAATGGTCCTTCTGTTGATCCTGTCAGTCTTACTTTTGAAA
  GAAGATGTCCGTGGGAGTGCACAGTCCAGTGAGAGGAGGGTGGTGGCTCACATGCCGGGT
  GACATCATTATTGGAGCTCTCTTTTCTGTTCATCACCAGCCTACTGTGGACAAAGTTCAT
  GAGAGGAAGTGTGGGGCGGTCCGTGAACAGTATGGCATTCAGAGAGTGGAGGCCATGCTG
  CATACCCTGGAAAGGATCAATTCAGACCCCACACTCTTGCCCAACATCACACTGNGCTGT
  GAGATAAGGGACTCCTGCTGGCATTCGGCTGTGGCCCTAGAGCAGAGCATTGAGTTCATA
  AGAGATTCCCTCATTTCTTCAGAAGAGGAAGAGGCTTGGTACGCTGTGTGGAGGGCTCCT
  CCTCTTCCTTCCGCTCCAGAAGCCCCATAGTAGGGTCATTGGCCCTGGCTTCAGATCTGG
  AGCCATTTCAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000842
• Insert Size: 6000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000842.1, NP_000833.1
• RefSeq Size: 4518 bp
• RefSeq ORF: 3543 bp
• Locus ID: 2915
• Cytogenetics: 11q14.2-q14.3
• Domains: 7tm_3, ANF_receptor
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Gap junction, Huntington's disease, Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (b) has a longer 5' UTR and lacks an in-frame exon in the 3' coding region, compared to variant a. The encoded isoform (b, also known as mGluR5a) is shorter, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.