GUCY1A1 (NM_000856) Human Untagged Clone

CAT#: SC119658

GUCY1A3 (untagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GUCY1A1
• Synonyms: GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3; GUCA3; GUCSA3; GUCY1A3; MYMY6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119658 sequence for NM_000856 edited (data generated by NextGen Sequencing)
  ATGTTCTGCACGAAGCTCAAGGATCTCAAGATCACAGGAGAGTGTCCTTTCTCCTTACTG
  GCACCAGGTCAAGTTCCTAACGAGTCTTCAGAGGAGGCAGCAGGAAGCTCAGAGAGCTGC
  AAAGCAACCGTGCCCATCTGTCAAGACATTCCTGAGAAGAACATACAAGAAAGTCTTCCT
  CAAAGAAAAACCAGTCGGAGCCGAGTCTATCTTCACACTTTGGCAGAGAGTATTTGCAAA
  CTGATTTTCCCAGAGTTTGAACGGCTGAATGTTGCACTTCAGAGAACATTGGCAAAGCAC
  AAAATAAAAGAAAGCAGGAAATCTTTGGAAAGAGAAGACTTTGAAAAAACAATTGCAGAG
  CAAGCAGTTGCAGCAGGAGTTCCAGTGGAGGTTATCAAAGAATCTCTTGGTGAAGAGGTT
  TTTAAAATATGTTACGAGGAAGATGAAAACATCCTTGGGGTGGTTGGAGGCACCCTTAAA
  GATTTTTTAAACAGCTTCAGTACCCTTCTGAAACAGAGCAGCCATTGCCAAGAAGCAGGA
  AAAAGGGGCAGGCTTGAGGACGCCTCCATTCTATGCCTGGATAAGGAGGATGATTTTCTA
  CATGTTTACTACTTCTTCCCTAAGAGAACCACCTCCCTGATTCTTCCCGGCATCATAAAG
  GCAGCTGCTCACGTATTATATGAAACGGAAGTGGAAGTGTCGTTAATGCCTCCCTGCTTC
  CATAATGATTGCAGCGAGTTTGTGAATCAGCCCTACTTGTTGTACTCCGTTCACATGAAA
  AGCACCAAGCCATCCCTGTCCCCCAGCAAACCCCAGTCCTCGCTGGTGATTCCCACATCG
  CTATTCTGCAAGACATTTCCATTCCATTTCATGTTTGACAAAGATATGACAATTCTGCAA
  TTTGGCAATGGCATCAGAAGGCTGATGAACAGGAGAGACTTTCAAGGAAAGCCTAATTTT
  GAAGAATACTTTGAAATTCTGACTCCAAAAATCAACCAGACGTTTAGCGGGATCATGACT
  ATGTTGAATATGCAGTTTGTTGTACGAGTGAGGAGATGGGACAACTCTGTGAAGAAATCT
  TCAAGGGTTATGGACCTCAAAGGCCAAATGATCTACATTGTTGAATCCAGTGCAATCTTG
  TTTTTGGGGTCACCCTGTGTGGACAGATTAGAAGATTTTACAGGACGAGGGCTCTACCTC
  TCAGACATCCCAATTCACAATGCACTGAGGGATGTGGTCTTAATAGGGGAACAAGCCCGA
  GCTCAAGATGGCCTGAAGAAGAGGCTGGGGAAGCTGAAGGCTACCCTTGAGCAAGCCCAC
  CAAGCCCTGGAGGAGGAGAAGAAAAAGACAGTAGACCTTCTGTGCTCCATATTTCCCTGT
  GAGGTTGCTCAGCAGCTGTGGCAAGGGCAAGTTGTGCAAGCCAAGAAGTTCAGTAATGTC
  ACCATGCTCTTCTCAGACATCGTTGGGTTCACTGCCATCTGCTCCCAGTGCTCACCGCTG
  CAGGTCATCACCATGCTCAATGCACTGTACACTCGCTTCGACCAGCAGTGTGGAGAGCTG
  GATGTCTACAAGGTGGAGACCATTGGCGATGCCTATTGTGTAGCTGGGGGATTACACAAA
  GAGAGTGATACTCATGCTGTTCAGATAGCGCTGATGGCCCTGAAGATGATGGAGCTCTCT
  GATGAAGTTATGTCTCCCCATGGAGAACCTATCAAGATGCGAATTGGACTGCACTCTGGA
  TCAGTTTTTGCTGGCGTCGTTGGAGTTAAAATGCCCCGTTACTGTCTTTTTGGAAACAAT
  GTCACTCTGGCTAACAAATTTGAGTCCTGCAGTGTACCACGAAAAATCAATGTCAGCCCA
  ACAACTTACAGATTACTCAAAGACTGTCCTGGTTTCGTGTTTACCCCTCGATCAAGGGAG
  GAACTTCCACCAAACTTCCCTAGTGAAATCCCCGGAATCTGCCATTTTCTGGATGCTTAC
  CAACAAGGAACAAACTCAAAACCATGCTTCCAAAAGAAAGATGTGGAAGATGGCAATGCC
  AATTTTTTAGGCAAAGCATCAGGAATAGATTAG
  
  Clone variation with respect to NM_000856.4
  1074 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000856 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGGGAGACACAGA
  CACAGAGAGACAAAGGCAAGGAGGACTGTCTGGGAGCCACGCGGGCGATACAGTTTCCGA
  GGCACGCCGCGTCCCGCCTAGCCTGTTGAACAGGTAGACATGAGCGACCCAAGCTGCGGA
  TTTGCGAGGCGCGCCCTGGAGCTGCTAGAGATCCGGAAGCACAGCCCCGAGGTGTGCGAA
  GCCACCAATCTCATATAAGAACTACAGCTCATCAGGAGGAGATCGCAGCAGGGTAAGAGA
  CACCAACACCATGTTCTGCACGAAGCTCAAGGATCTCAAGATCACAGGAGAGTGTCCTTT
  CTCCTTACTGGCACCAGGTCAAGTTCCTAACGAGTCTTCAGAGGAGGCAGCAGGAAGCTC
  AGAGAGCTGCAAAGCAACCGTGCCCATCTGTCAAGACATTCCTGAGAAGAACATACAAGA
  AAGTCTTCCTCAAAGAAAAACCAGTCGGAGCCGAGTCTATCTTCACACTTTGGCAGAGAG
  TATTTGCAAACTGATTTTCCCAGAGTTTGAACGGCTGAATGTTGCACTTCAGAGAACATT
  GGCAAAGCACAAAATAAAAGAAAGCAGGAAATCTTTGGAAAGAGAAGACTTTGAAAAAAC
  AATTGCAGAGCAAGCAGTTGCAGCAGGAGTTCCAGTGGAGGTTATCAAAGAATCTCTTGG
  TGAAAGAGTTTTTAAAATATGTTACGAGGAAGATGAAAACATCTTGGGGGTGTNTGCAGG
  CACCCTTAAAGATTTTNTAAACAGCTTCAGTACCCTTCTGAACAGAGCAGCCATTGCCAA
  GAAGCAGAAAAGGGGCAGCTTGAGGACGCCTCATTCTATGCTGATAGGNGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000856 unedited
  TTTAAAAAAANNNTTTCTTATGNACCGCGGNCCCGCATTTTANGATCGAGTTTTTTTTTT
  TTTTTTTGAAAAGCTGAAGTACTGAAGTGAGTACATTTTGTCATGTTAAACAGGAGAAAG
  ATTGTGACTTGGCTCCTGAAATTTTAATACTGCTTGTTAGCCATCTACAATCCCTAAAGT
  GCTTTCAGAGGCTCTACACATCTTCAATGAGTCAAACCCCAAAGACTTATAAATAGGTAT
  ATAGGTTGCTAATCTATTCCTGATGCTTTGCCTAAAAAATTGGCATTGCCATCTTCCACA
  TCTTTCTTTTGGAAGCATGGTTTTGAGTTTGTTCCTTGTTGGTAAGCATCCAGAAAATGG
  CAGATTCCGGGGATTTCACTANGGAAGTTTGGTGGAAGTTCCTCCCTTGATCGAGGNGGT
  AACACGANACCAGGACAGTCTTTGAGTAATCTGTAAAGTGTTGGGCTGACATTGATTTTT
  CGTGGTACACTGCANGACTCAAATTTGTAGCCAGAGTGACATTGNNTCCAAAAGACAGTA
  ACGGNGCATTTACTNCAACGACGCCAGCAAAACTGATCCAGAGTGCAGTCCAATTCGCAT
  CTTGATAGTNCTCCATGGNGAGACATAACTTCATAGAAAGCTCCATCATCTTTAGGGCCA
  TCANCGCTATCTGAAAACATGAGTTCACTCTCTTGGGTAATTCCCCAGCTTCCCATAGGC
  ATTGCCAATGGTCTCCACCTTGTGAAATCCAGCTTTCCACTGNTGGTCAAACGAGGGCCC
  AGGCCTTGACCTGGGGATGATTCAACGGGTAACCCTTGGACCAATGCCTTGGACCCACAA
  TTCTGAAAAAACTGGGGACCTTCTGAATTTTGGGTTGCCACATGCCTTTGCCAATTGTTG
  CCCCCCTCCGGAAAATTGGGCCCAAAGGTCCTGTGTTTTTTTTCCCTCCAAGCTGGGGGG
  TTTGCAGGGGCCCTAATTCCCACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000856
• Insert Size: 2570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000856.2, NP_000847.2
• RefSeq Size: 3015 bp
• RefSeq ORF: 2073 bp
• Locus ID: 2982
• Cytogenetics: 4q32.1
• Domains: CYCc
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
• Gene Summary: 'Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (A). Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.