CHRND (NM_000751) Human Untagged Clone

CAT#: SC119700

CHRND (untagged)-Human cholinergic receptor, nicotinic, delta (CHRND)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRND
• Synonyms: ACHRD; CMS2A; CMS3A; CMS3B; CMS3C; FCCMS; SCCMS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000751, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGGGCCAGTGCTGACACTGGGGCTGCTGGCTGCCCTGGCGGTGTGTGGCAGCTGGGGGCTGAACG
  AGGAGGAGCGGCTGATCCGGCACCTGTTTCAAGAGAAGGGCTACAACAAGGAGCTCCGGCCCGTGGCACA
  CAAAGAGGAGAGTGTGGACGTTGCCCTGGCCCTCACACTCTCCAACCTCATCTCCCTGAAAGAAGTTGAG
  GAGACCCTCACTACCAATGTGTGGATAGAGCACGGCTGGACAGACAACCGGCTGAAGTGGAATGCTGAAG
  AATTTGGAAACATCAGTGTCCTGCGCCTCCCCCCGGACATGGTGTGGCTCCCAGAGATTGTGCTGGAGAA
  CAACAATGACGGCTCCTTCCAGATCTCCTACTCCTGCAACGTGCTTGTCTACCACTACGGCTTCGTGTAC
  TGGCTGCCACCTGCCATCTTCCGCTCCTCCTGCCCCATCTCTGTCACCTATTTCCCCTTCGACTGGCAGA
  ACTGCTCCCTCAAGTTCAGTTCCCTCAAGTATACGGCCAAAGAGATCACCCTGAGCCTGAAACAGGATGC
  CAAGGAGAACCGCACCTACCCCGTGGAGTGGATCATCATTGATCCTGAAGGCTTCACAGAGAACGGGGAG
  TGGGAGATAGTCCACCGGCCGGCCAGGGTCAACGTGGACCCCAGAGCCCCTCTGGACAGCCCCAGCCGCC
  AGGACATCACCTTCTACCTCATCATCCGCCGCAAGCCCCTCTTCTACATCATCAACATCCTGGTGCCCTG
  CGTGCTCATCTCCTTCATGGTCAACCTGGTCTTCTACCTACCGGCTGACAGTGGTGAGAAGACATCAGTG
  GCCATCTCGGTGCTCCTGGCTCAGTCTGTCTTCCTGCTGCTCATCTCCAAGCGTCTGCCTGCCACATCCA
  TGGCCATCCCCCTTATCGGCAAGTTCCTGCTCTTCGGCATGGTGCTGGTCACCATGGTTGTGGTGATCTG
  TGTCATCGTGCTCAACATCCACTTCCGAACACCCAGCACCCATGTGCTGTCTGAGGGGGTCAAGAAGCTC
  TTCCTGGAGACCCTGCCGGAGCTCCTGCACATGTCCCGCCCAGCAGAGGATGGACCCAGCCCTGGGGCCC
  TGGTGCGGAGGAGCAGCTCCCTGGGATACATCTCCAAGGCCGAGGAGTACTTCCTGCTCAAGTCCCGCAG
  TGACCTCATGTTCGAGAAGCAGTCAGAGCGGCATGGGCTGGCCAGGCGCCTCACCACTGCACGCCGGCCC
  CCAGCAAGCTCTGAGCAGGCCCAGCAGGAACTCTTCAATGAGCTGAAGCCAGCTGTGGATGGGGCAAACT
  TCATTGTTAACCACATGAGGGACCAGAACAATTACAATGAGGAGAAAGACAGCTGGAACCGAGTGGCCCG
  CACAGTGGACCGCCTCTGCCTGTTTGTGGTGACGCCTGTCATGGTGGTGGGCACAGCCTGGATCTTCCTG
  CAGGGCGTTTACAACCAGCCACCACCCCAGCCTTTTCCTGGGGACCCCTACTCCTACAACGTGCAGGACA
  AGCGCTTCATCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000751 unedited
  AGGTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGGGAGGGGC
  CGGTGCTGACACTGGGGCTGCTGGCTGCCCTGGCGGTGTGTGGCAGCTGGGGGCTGAACG
  AGGAGGAGCGGCTGATCCGGCACCTGTTTCAAGAGAAGGGCTACAACAAGGAGCTCCGGC
  CCGTGGCACACAAAGAGGAGAGTGTGGACGTTGCCCTGGCCCTCACACTCTCCAACCTCA
  TCTCCCTGAAAGAAGTTGAGGAGACCCTCACTACCAATGTGTGGATAGAGCACGGCTGGA
  CAGACAACCGGCTGAAGTGGAATGCTGAAGAATTTGGAAACATCAGTGTCCTGCGCCTCC
  CCCCGGACATGGTGTGGCTCCCAGAGATTGTGCTGGAGAACAACAATGACGGCTCCTTCC
  AGATCTCCTACTCCTGCAACGTGCTTGTCTACCACTACGGCTTCGTGTACTGGCTGCCAC
  CTGCCATCTTCCGCTCCTCCTGCCCCATCTCTGTCACCTATTTCCCCTTCGACTGGCAGA
  ACTGCTCCCTCAAGTTCAGTTCCCTCAAGTATACGGCCAAAGAGATCACCCTGAGCCTGA
  NACAGGATGCCAAGGAGAACCGCACCTACCCCGTGGAGTGGATCATCATTGATCCTGAAG
  GCTTCACAGAGAACGGGGAGTGGGAGATAGTCCACCGGGCCGCCAGGGTCAACGTGGACC
  CCAGAGCCCCTCTGGACAGCCCCAGCCGCCAGGACATCACCTTCTACCTCATCATCCGCC
  GCAAGCCCCTCTTCTAATCATCAACATCCCTGGTGCCCTGCGTGCTCATCTCCTTTATGG
  TCAACCTGGTCCTCTACCTAACCGCTGACAGTGGTGGAGAAGACATCAGTGGCCATCTCG
  GTGCCTACTGGCTCAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000751
• Insert Size: 2880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000751.1, NP_000742.1
• RefSeq Size: 1741 bp
• RefSeq ORF: 1554 bp
• Locus ID: 1144
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Gene Summary: 'The acetylcholine receptor of muscle has 5 subunits of 4 different types: 2 alpha and 1 each of beta, gamma and delta subunits. After acetylcholine binding, the receptor undergoes an extensive conformation change that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in this gene are a cause of multiple pterygium syndrome lethal type (MUPSL), congenital myasthenic syndrome slow-channel type (SCCMS), and congenital myasthenic syndrome fast-channel type (FCCMS). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.