Muscarinic Acetylcholine Receptor M3 (CHRM3) (NM_000740) Human Untagged Clone

CAT#: SC119736

CHRM3 (untagged)-Human cholinergic receptor, muscarinic 3 (CHRM3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRM3
• Synonyms: EGBRS; HM3; PBS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119736 sequence for NM_000740 edited (data generated by NextGen Sequencing)
  ATGACCTTGCACAATAACAGTACAACCTCGCCTTTGTTTCCAAACATCAGCTCCTCCTGG
  ATACACAGCCCCTCCGATGCAGGGCTGCCCCCGGGAACCGTCACTCATTTCGGCAGCTAC
  AATGTTTCTCGAGCAGCTGGCAATTTCTCCTCTCCAGACGGTACCACCGATGACCCTCTG
  GGAGGTCATACCGTCTGGCAAGTGGTCTTCATCGCTTTCTTAACGGGCATCCTGGCCTTG
  GTGACCATCATCGGCAACATCCTGGTAATTGTGTCATTTAAGGTCAACAAGCAGCTGAAG
  ACGGTCAACAACTACTTCCTCTTAAGCCTGGCCTGTGCCGATCTGATTATCGGGGTCATT
  TCAATGAATCTGTTTACGACCTACATCATCATGAATCGATGGGCCTTAGGGAACTTGGCC
  TGTGACCTCTGGCTTGCCATTGACTACGTAGCCAGCAATGCCTCTGTTATGAATCTTCTG
  GTCATCAGCTTTGACAGATACTTTTCCATCACGAGGCCGCTCACGTACCGAGCCAAACGA
  ACAACAAAGAGAGCCGGTGTGATGATCGGTCTGGCTTGGGTCATCTCCTTTGTCCTTTGG
  GCTCCTGCCATCTTGTTCTGGCAATACTTTGTTGGAAAGAGAACTGTGCCTCCGGGAGAG
  TGCTTCATTCAGTTCCTCAGTGAGCCCACCATTACTTTTGGCACAGCCATCGCTGCTTTT
  TATATGCCTGTCACCATTATGACTATTTTATACTGGAGGATCTATAAGGAAACTGAAAAG
  CGTACCAAAGAGCTTGCTGGCCTGCAAGCCTCTGGGACAGAGGCAGAGACAGAAAACTTT
  GTCCACCCCACGGGCAGTTCTCGAAGCTGCAGCAGTTACGAACTTCAACAGCAAAGCATG
  AAACGCTCCAACAGGAGGAAGTATGGCCGCTGCCACTTCTGGTTCACAACCAAGAGCTGG
  AAACCCAGCTCCGAGCAGATGGACCAAGACCACAGCAGCAGTGACAGTTGGAACAACAAT
  GATGCTGCTGCCTCCCTGGAGAACTCCGCCTCCTCCGACGAGGAGGACATTGGCTCCGAG
  ACGAGAGCCATCTACTCCATCGTGCTCAAGCTTCCGGGTCACAGCACCATCCTCAACTCC
  ACCAAGTTACCCTCATCGGACAACCTGCAGGTGCCTGAGGAGGAGCTGGGGATGGTGGAC
  TTGGAGAGGAAAGCCGACAAGCTGCAGGCCCAGAAGAGCGTGGACGATGGAGGCAGTTTT
  CCAAAAAGCTTCTCCAAGCTTCCCATCCAGCTAGAGTCAGCCGTGGACACAGCTAAGACT
  TCTGACGTCAACTCCTCAGTGGGTAAGAGCACGGCCACTCTACCTCTGTCCTTCAAGGAA
  GCCACTCTGGCCAAGAGGTTTGCTCTGAAGACCAGAAGTCAGATCACTAAGCGGAAAAGG
  ATGTCCCTGGTCAAGGAGAAGAAAGCGGCCCAGACCCTCAGTGCGATCTTGCTTGCCTTC
  ATCATCACTTGGACCCCATACAACATCATGGTTCTGGTGAACACCTTTTGTGACAGCTGC
  ATACCCAAAACCTTTTGGAATCTGGGCTACTGGCTGTGCTACATCAACAGCACCGTGAAC
  CCCGTGTGCTATGCTCTGTGCAACAAAACATTCAGAACCACTTTCAAGATGCTGCTGCTG
  TGCCAGTGTGACAAAAAAAAGAGGCGCAAGCAGCAGTACCAGCAGAGACAGTCGGTCATT
  TTTCACAAGCGCGCACCCGAGCAGGCCTTGTAG
  
  Clone variation with respect to NM_000740.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000740 unedited
  CCCGCCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAG
  CTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTC
  GGCACGAGGCCTCGTGCCGAATTCGGCACGAGGTTGCCCTGGCAGAGGGAACCTACCCAG
  TCCATTGCTGCCTGCTACAAGATATGAACAGTAATGGCACATATTTTGGTTATGAGTCAC
  TCAGTGGACTGTGGATTGAATGAACTGTATCCATCCCCATCATGATGTACAGAACCAAGT
  CTCTTCACTACACTTGTGTTCTGATTAGTGGCCAAATAAATGACAGTCAGAACTTCAGCT
  AAGGTACAATAAGGTTTTGCTGTGGCGTGGCACCTGGTCTCTTTCTAGAAGGAAAGTTCA
  ACATACAGCACAATTCTGGACACATTGTATTGGTTTGATGCTCCTACCTGGAACAGGTGG
  AGCTGGTCTCTTGGGCAGCCTGACATCTGGTCCACTCCTCTGTCCTATGCCGGGATCATC
  ATGACCGTAGAGATTATGTCACTGTTTTGCATCCTTGTTACATAACTCAGTTCCTGGTAG
  ATTACTATGTCAGAGAGTCACAATGACCTTGCACAATAACAGTACAACCTCGCCTTTGTT
  TCCAAACATCAGCTCCTCCTGGATACACAGCCCCTCCGATGCANGGCTGCCCCCGGGAAC
  CGTCACTCATTTCGGCAGCTACAATGTTTCTCGAGCAGCTGGCAATTTCTCCTCTCCAGA
  CGGTACCACCGATGACCCTCTGGGGAGTCATACCGTCTGGCAAGTGTCTTCATCGCTTTT
  TTACGGGCATTCTGGCCTTGGTGACCATCATCGGCCACATCCTGGTAATTGNGTTATTTT
  AAGGCAACAAGCAGTTGAAGACGGNCACAACTACTTCTCCTAAACCTGGCCTGGGCCGAT
  CTGATTATGGGGGCACTTCAATGAATGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000740 unedited
  TGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTGAATTGGTAT
  TGACTTTTTCTATTAAAATGGGTTTATTCAATGGATCAGTAAACAGGCAGCTTCTTTCAC
  ATCTGGGTGATAAAACCATTTTTATCATCACCAGAAGTCACCCCGCCCTCGCCTTCCTCC
  TCCTAAGGTCTGTGGGTTGATGTGTGCGTTTTGTCACTGCTATTGATACAACCTCATTCT
  ACAAGGCCTGCTCGGGTGCGCGCTTGTGAAAAATGACCGACTGTCTCTGCTGGTACTGCT
  GCTTGCGCCTCTTTTTTTTGTCACACTGGCACAGCAGCAGCATCTTGAAAGTGGTTCTGA
  ATGTTTTGTTGCACAGAGCATAGCACACGGGGTTCACGGTGCTGTTGATGTAGCACAGCC
  AGTAGCCCAGATTCCAAAAGGTTTTGGGTATGCAGCTGTCACAAAAGGTGTTCACCAGAA
  CCATGATGTTGTATGGGGTCCAAGTGATGATGAAGGCAAGCAAGATCGCACTGAGGGTCT
  GGGCCGCTTTCTTCTCCTTGACCAGGGACATCCTTTTCCGCTTAGTGATCTGACTTCTGG
  TCTTCAGAGCAAACCTCTTGGCCAGAGTGGCTTCCTTGAANGACAGAGGTANAGTGGCCG
  TGCTCTTACCCACTGANGAGTTGACGTCAGAAGTCTTACCTGTGTCCACGGCTGACTCTA
  CCTGGATGGGAAACCTTGGAGAAGCTTTTTGGAAAACTGGCTTCATCGTTCCCGCTTTTT
  TGGCCTGCAGCTTGTCGGCTTTCCTTTCAAGTCCACATCCCCAGTTCTTCTTAAGACCTT
  GAGGGTGTCCCATGAAGGAACTTGGGGGAATTAGGAATGGCCTTTCACCCGAACTCTGCA
  CAAAGGATAAATGCTTCCTTCTGAACCAGGTCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000740
• Insert Size: 2630 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000740.2, NP_000731.1
• RefSeq Size: 2757 bp
• RefSeq ORF: 1773 bp
• Locus ID: 1131
• Cytogenetics: 1q43
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• Gene Summary: 'The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]'
  Transcript Variant: This variant (2) differs in the 5' UTR and represents use of an alternate promoter, compared to variant 1. Both variants 1 and 2 encode the same protein. The 5' end of this transcript contains L1 element sequence, initiates from an L1 element promoter and expression was predominantly found in placenta. (PMID: 19669628). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.