Muscarinic Acetylcholine Receptor M4 (CHRM4) (NM_000741) Human Untagged Clone

CAT#: SC119737

CHRM4 (untagged)-Human cholinergic receptor, muscarinic 4 (CHRM4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRM4
• Synonyms: HM4; M4R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000741, RT-PCR generated
  ATGGCCAACTTCACACCTGTCAATGGCAGCTCGGGCAATCAGTCCGTGCGCCTGGTCACG
  TCATCATCCCACAATCGCTATGAGACGGTGGAAATGGTCTTCATTGCCACAGTGACAGGC
  TCCCTGAGCCTGGTGACTGTCGTGGGCAACATCCTGGTGATGCTGTCCATCAAGGTCAAC
  AGGCAGCTGCAGACAGTCAACAACTACTTCCTCTTCAGCCTGGCGTGTGCTGATCTCATC
  ATAGGCGCCTTCTCCATGAACCTCTACACCGTGTACATCATCAAGGGCTACTGGCCCCTG
  GGCGCCGTGGTCTGCGACCTGTGGCTGGCCCTGGACTACGTGGTGAGCAACGCCTCCGTC
  ATGAACCTTCTCATCATCAGCTTTGACCGCTACTTCTGCGTCACCAAGCCTCTCACCTAC
  CCTGCCCGGCGCACCACCAAGATGGCAGGCCTCATGATTGCTGCTGCCTGGGTACTGTCC
  TTCGTGCTCTGGGCGCCTGCCATCTTGTTCTGGCAGTTTGTGGTGGGTAAGCGGACGGTG
  CCCGACAACCAGTGCTTCATCCAGTTCCTGTCCAACCCAGCAGTGACCTTTGGCACAGCC
  ATTGCTGCCTTCTACCTGCCTGTGGTCATCATGACGGTGCTGTACATCCACATCTCCCTG
  GCCAGTCGCAGCCGAGTCCACAAGCACCGGCCCGAGGGCCCGAAGGAGAAGAAAGCCAAG
  ACGCTGGCCTTCCTCAAGAGCCCACTAATGAAGCAGAGCGTCAAGAAGCCCCCGCCCGGG
  GAGGCCGCCCGGGAGGAGCTGCGCAATGGCAAGCTGGAGGAGGCCCCCCCGCCAGCGTTG
  CCACCGCCACCGCGCCCCGTGGCTGATAAGGACACTTCCAATGAGTCCAGCTCAGGCAGT
  GCCACCCAGAACACCAAGGAACGCCCAGCCACAGAGCTGTCCACCACAGAGGCCACCACG
  CCCGCCATGCCCGCCCCTCCCCTGCAGCCGCGGGCCCTCAACCCAGCCTCCAGATGGTCC
  AAGATCCAGATTGTGACGAAGCAGACAGGCAATGAGTGTGTGACAGCCATTGAGATTGTG
  CCTGCCACGCCGGCTGGCATGCGCCCTGCGGCCAACGTGGCCCGCAAGTTCGCCAGCATC
  GCTCGCAACCAGGTGCGCAAGAAGCGGCAGATGGCGGCCCGGGAGCGCAAAGTGACACGA
  ACGATCTTTGCCATTCTGCTAGCCTTCATCCTCACCTGGACGCCCTACAACGTCATGGTC
  CTGGTGAACACCTTCTGCCAGAGCTGCATCCCTGACACGGTGTGGTCCATTGGCTACTGG
  CTCTGCTACGTCAACAGCACCATCAACCCTGCCTGCTATGCTCTGTGCAACGCCACCTTT
  AAAAAGACCTTCCGGCACCTGCTGCTGTGCCAGTATCGGAACATCGGCACTGCCAGGTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000741 unedited
  NGGTAATTTTAGATTTGTAAACGACTCATATAGGCGGCCGCCATGTGATGTGAATCTGCA
  GAATTCGGCTTCAACATGGCCAACTTCACACCTGTCAATGGCAGCTCGGGCAATCAGTCC
  GTGCGCCTGGTCACGTCATCATCCCACAATCGCTATGAGACGGTGGAAATGGTCTTCATT
  GCCACAGTGACAGGCTCCCTGAGCCTGGTGACTGTCGTGGGCAACATCCTGGTGATGCTG
  TCCATCAAGGTCAACAGGCAGCTGCAGACAGTCAACAACTACTTCCTCTTCAGCCTGGCG
  TGTGCTGATCTCATCATAGGCGCCTTCTCCATGAACCTCTACACCGTGTACATCATCAAG
  GGCTACTGGCCCCTGGGCGCCGTGGTCTGCGACCTGTGGCTGGCCCTGGACTACGTGGTG
  AGCAACGCCTCCGTCATGAACCTTCTCATCATCAGCTTTGGCCGCTACTTCTGCGTCACC
  AAGCCTCTCACCTACCCTGCCCGGCGCACCACCAAGATGGCAGGCCTCATGATTGCTGCT
  GCCTGGGTACTGTCCTTCGTGCTCTGGGCGCCTGCCATCTTGTTCTGGCAGTTTGTGGTG
  GGTAAGCGGACGGTGCCCGACAACCAGTGCTTCATCCAGTTCCTGTCCAACCCAGCAGTG
  ACCTTTGGCACGGCCATTGCTGCCTTCTACCTGCCTGTGGTCATCATGACGGTGCTGTAC
  ATCCACATCTCCCTGGCCAGTCGCAGCCGAGTCCACAAGCACCGGCCCGAGGGCCCGANA
  GAGAAGAAAGCCAAGACGCTGGCCTTCCTCAAGAGCCCCTAATGAAGCANAGCGTCAAGA
  AGCCCCCGCCCCGGGGAGTCCNGCCTGGNNAGNAGCTGCGNCATGNNCAGCTNGNNAGAG
  GCCCCCCCGNCAGCGCTGCCACCGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000741 unedited
  CTTTCCCNNAAATACTGTGNNACCGCGGCCGCTTACTAGATCGNAAGNCTTGNAATCGGT
  ACCGAGCTCGGATCCACTATAACGGCCGCCATGTGCTGGAATTCGGCTTCCTGCCTGCCT
  ACCTGGCAGTGCCGATGTTCCGATACTGGCACAGCAGCAGGTGCCGGAAGGTCTTTTTAA
  AGGTGGCGTTGCACAGAGCATAGCAGGCAGGGTTGATAGTGCTGTTGACGTAGCAGAGCC
  AGTAGCCAATGGACCACACCGTGTCAGGGATGCAGCTCTGGCAGAAGGTGTTCACCAGGA
  CCATGACGTTGTAGGGCGTCCAGGTGAGGATGAAGGCTAGCAGAATGGCAAAGATCGTTC
  GTGTCACTTTGCGCTCCCGGGCCGCCATCTGCCGCTTCTTGCGCACCTGGTTGCGAGCGA
  TGCTGGCGAACTTGCGGGCCACGTTGGCCGCAGGGCGCATGCCAGCCGGCGTGGCAGGCA
  CAATCTCAATGGCTGTCACACACTCATTGCCTGTCTGCTTCGTCACAATCTGGATCTTGG
  ACCATCTGGAGGCTGGGTTGAGGGCCCGCGGCTGCAGGGGAGGGGCGGGCATGGCGGGCG
  TGGTGGCCTCTGTGGTGGACAGCTCTGTGGCTGGGCGTTCCTTGGTGTTCTGGGTGGCAC
  TGCCTGAGCTGGACTCATTGGAAGTGTCCTTATCANCCACGGGGCGCGGTGGCGGTGGCA
  GCGCTGGCGGGGGGGCCTCCTCCAGCTTGCCATTGCCCAACTCCTCCCGGGCGGCCTCCC
  GGGCGGGGGCCTCTTTGACCCCTTGTTTCATTAATGGGCTCTTTGAGAAGGCCACGGTTT
  TGGGTTTTTCTTCCTTCCGGCCCTCGGCCGGTGCTTGGGGACTCG
  
• Restriction Sites: Please inquire
• ACCN: NM_000741
• Insert Size: 1550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000741.2, NP_000732.2
• RefSeq Size: 1468 bp
• RefSeq ORF: 1440 bp
• Locus ID: 1132
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• Protein Pathways: Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• Gene Summary: 'The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. [provided by RefSeq, Jul 2008]'