Adenylate Kinase 1 (AK1) (NM_000476) Human Untagged Clone

CAT#: SC119850

AK1 (untagged)-Human adenylate kinase 1 (AK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AK1
• Synonyms: HTL-S-58j
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119850 sequence for NM_000476 edited (data generated by NextGen Sequencing)
  ATGGAAGAGAAGCTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGG
  AAGGGCACCCAGTGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGG
  GACCTCCTGCGGTCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATC
  ATGGAGAAGGGGCAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATG
  GTGGCCAAAGTCAATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAG
  CAAGGAGAAGAGTTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCA
  GGCCCTGAGACCATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGAC
  GACAATGAGGAGACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTC
  ATCGCCTTCTATGAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGAC
  AGTGTCTTCTCCCAGGTCTGCACCCACCTGGACGCCCTAAAGTAG
  
  Clone variation with respect to NM_000476.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000476 unedited
  GTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCACGCACTGGC
  CCCGGCGCCCACCCGCACCCCTCCCCAAAGCACTGACACGGCTCCCGGGACCTCGGCAGG
  ATGGAAGAGAAGCTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGG
  AAGGGCACCCAGTGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGG
  GACCTCCTGCGGTCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATC
  ATGGAGAAGGGGCAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATG
  GTGGCCAAAGTCAATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAG
  CAAGGAGAAGAGTTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCA
  GGCCCTGAGACCATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGAC
  CGACATGAGGAGACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTC
  ATCGCCTTCTATGAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGNAGGCTCCGTGGAC
  AGTGTCTTCTCCAAGTCTGCACCACCTGNACGCCCTAAGTAGCAACGCTGGAGCCGCTTC
  CCCAGCTCAAAGCCCCGCCCCACCCCGTCCTGATTAGAGGTCCCTCTGGCCTGAGCGCAG
  CGCCTNCACCCTGGCCTGCTGAGCACAGACGGAGGAAGCCGCTTTATCTGTTTCATGGAC
  AGCTGAGCCCTAAGGAAATTTCTTAGGAAAAAANNNNNNNNNNANNNNNNNNNNNNANNN
  ANANANAAAAAAAAANCCCTTCGCTTTAGTTGGGGCCCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000476 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTCCTTAAAAATTCCTTTAGGGCTCACCTGCCCATAAAAACA
  GGATAAGCGGTTTCCTCCGTTTGGGCTAAAAAGGGCAGGGGGGAGGCCCTGCCCTAAGGC
  CAGAAGGACCTTTAATAAGAACGGGGGGGGGCGGGGCTTTAACCTGGGAAAGCGGTTCCA
  GCGTTGTTACTTTAGGGCGTCCAGGGGGGGGCAAACCTGGAAAAAAACACTGTCCACGGA
  CCCCTAAGGGTTAACCTTGCCCACAATGCCACGTTTTTAATAAAAGGCAAAAACGGGTTC
  TGGGGCCTTGAAAAAGGTCTCCACCCGTTTTTTGAGGGCCTCCTCATTGGCGCCCACACC
  CCCGCTGGTCTCTCCACGTTTAAAAACCCGCTGGGTCATGGTCTAAGGGCCTGCGTCCAC
  ATACAGAAGAAGGGGGGGCTGTCCAATCCGGCGTTAAAACTTTTTTCCTTGCTGCACCTC
  CCGGGGGTACCCATCAATCAGGAACCCTTTGAAAGTATTGACTTTGGCCACCATGGCATC
  CCGGAGCATGTCCAACACTGTCTCCAGGGGAACCAGCTGCCCCTTCTCCATGATTTCCAA
  CAGCTTCTTGCCCCTGGCCGACCCTGAGCTAACCTCGGACCCAGGAGGCCCCCGGGGGAA
  AAGTGGGTTGACCCATACTTTTGGAGATCTTTTAACACTGGGTGCCCTTCCCTGAACCAG
  GCCCACCCACCACAAAGAGGATCTTGGNTTTNTTAAGATTCTCTTCCCTCCTGCCGAGGC
  CCGGGAACCCCTGTAAATGCTTTTGGGAAGGGTGCGGGGGGCCCCCGGGCCAAGGCGTGC
  CCTCTGCCGAATATCGGGCCCCCTAAAGGGAGTCCATTACAAAATTTGCAGGTACAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000476
• Insert Size: 870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000476.1, NP_000467.1
• RefSeq Size: 2271 bp
• RefSeq ORF: 585 bp
• Locus ID: 203
• Cytogenetics: 9q34.11
• Domains: ADK
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: 'This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the shorter isoform (1). Both variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.