Adenylate Kinase 1 (AK1) (NM_000476) Human Untagged Clone

CAT#: SC119850

AK1 (untagged)-Human adenylate kinase 1 (AK1)


  "NM_000476" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol AK1
Synonyms HTL-S-58j
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119850 sequence for NM_000476 edited (data generated by NextGen Sequencing)
ATGGAAGAGAAGCTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGG
AAGGGCACCCAGTGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGG
GACCTCCTGCGGTCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATC
ATGGAGAAGGGGCAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATG
GTGGCCAAAGTCAATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAG
CAAGGAGAAGAGTTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCA
GGCCCTGAGACCATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGAC
GACAATGAGGAGACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTC
ATCGCCTTCTATGAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGAC
AGTGTCTTCTCCCAGGTCTGCACCCACCTGGACGCCCTAAAGTAG

Clone variation with respect to NM_000476.2
>OriGene 5' read for NM_000476 unedited
GTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCACGCACTGGC
CCCGGCGCCCACCCGCACCCCTCCCCAAAGCACTGACACGGCTCCCGGGACCTCGGCAGG
ATGGAAGAGAAGCTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGG
AAGGGCACCCAGTGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGG
GACCTCCTGCGGTCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATC
ATGGAGAAGGGGCAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATG
GTGGCCAAAGTCAATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAG
CAAGGAGAAGAGTTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCA
GGCCCTGAGACCATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGAC
CGACATGAGGAGACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTC
ATCGCCTTCTATGAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGNAGGCTCCGTGGAC
AGTGTCTTCTCCAAGTCTGCACCACCTGNACGCCCTAAGTAGCAACGCTGGAGCCGCTTC
CCCAGCTCAAAGCCCCGCCCCACCCCGTCCTGATTAGAGGTCCCTCTGGCCTGAGCGCAG
CGCCTNCACCCTGGCCTGCTGAGCACAGACGGAGGAAGCCGCTTTATCTGTTTCATGGAC
AGCTGAGCCCTAAGGAAATTTCTTAGGAAAAAANNNNNNNNNNANNNNNNNNNNNNANNN
ANANANAAAAAAAAANCCCTTCGCTTTAGTTGGGGCCCCG
>OriGene 3' read for NM_000476 unedited
CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTCCTTAAAAATTCCTTTAGGGCTCACCTGCCCATAAAAACA
GGATAAGCGGTTTCCTCCGTTTGGGCTAAAAAGGGCAGGGGGGAGGCCCTGCCCTAAGGC
CAGAAGGACCTTTAATAAGAACGGGGGGGGGCGGGGCTTTAACCTGGGAAAGCGGTTCCA
GCGTTGTTACTTTAGGGCGTCCAGGGGGGGGCAAACCTGGAAAAAAACACTGTCCACGGA
CCCCTAAGGGTTAACCTTGCCCACAATGCCACGTTTTTAATAAAAGGCAAAAACGGGTTC
TGGGGCCTTGAAAAAGGTCTCCACCCGTTTTTTGAGGGCCTCCTCATTGGCGCCCACACC
CCCGCTGGTCTCTCCACGTTTAAAAACCCGCTGGGTCATGGTCTAAGGGCCTGCGTCCAC
ATACAGAAGAAGGGGGGGCTGTCCAATCCGGCGTTAAAACTTTTTTCCTTGCTGCACCTC
CCGGGGGTACCCATCAATCAGGAACCCTTTGAAAGTATTGACTTTGGCCACCATGGCATC
CCGGAGCATGTCCAACACTGTCTCCAGGGGAACCAGCTGCCCCTTCTCCATGATTTCCAA
CAGCTTCTTGCCCCTGGCCGACCCTGAGCTAACCTCGGACCCAGGAGGCCCCCGGGGGAA
AAGTGGGTTGACCCATACTTTTGGAGATCTTTTAACACTGGGTGCCCTTCCCTGAACCAG
GCCCACCCACCACAAAGAGGATCTTGGNTTTNTTAAGATTCTCTTCCCTCCTGCCGAGGC
CCGGGAACCCCTGTAAATGCTTTTGGGAAGGGTGCGGGGGGCCCCCGGGCCAAGGCGTGC
CCTCTGCCGAATATCGGGCCCCCTAAAGGGAGTCCATTACAAAATTTGCAGGTACAAAA
Restriction Sites NotI-NotI     
ACCN NM_000476
Insert Size 870 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000476.1, NP_000467.1
RefSeq Size 2271 bp
RefSeq ORF 585 bp
Locus ID 203
Cytogenetics 9q34.11
Domains ADK
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Purine metabolism
Gene Summary 'This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the shorter isoform (1). Both variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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