Plasminogen (PLG) (NM_000301) Human Untagged Clone

CAT#: SC119955

PLG (untagged)-Human plasminogen (PLG), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLG
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119955 sequence for NM_000301 edited (data generated by NextGen Sequencing)
  ATGGAACATAAGGAAGTGGTTCTTCTACTTCTTTTATTTCTGAAATCAGGTCAAGGAGAG
  CCTCTGGATGACTATGTGAATACCCAGGGGGCTTCACTGTTCAGTGTCACTAAGAAGCAG
  CTGGGAGCAGGAAGTATAGAAGAATGTGCAGCAAAATGTGAGGAGGACGAAGAATTCACC
  TGCAGGGCATTCCAATATCACAGTAAAGAGCAACAATGTGTGATAATGGCTGAAAACAGG
  AAGTCCTCCATAATCATTAGGATGAGAGATGTAGTTTTATTTGAAAAGAAAGTGTATCTC
  TCAGAGTGCAAGACTGGGAATGGAAAGAACTACAGAGGGACGATGTCCAAAACAAAAAAT
  GGCATCACCTGTCAAAAATGGAGTTCCACTTCTCCCCACAGACCTAGATTCTCACCTGCT
  ACACACCCCTCAGAGGGACTGGAGGAGAACTACTGCAGGAATCCAGACAACGATCCGCAG
  GGGCCCTGGTGCTATACTACTGATCCAGAAAAGAGATATGACTACTGCGACATTCTTGAG
  TGTGAAGAGGAATGTATGCATTGCAGTGGAGAAAACTATGACGGCAAAATTTCCAAGACC
  ATGTCTGGACTGGAATGCCAGGCCTGGGACTCTCAGAGCCCACACGCTCATGGATACATT
  CCTTCCAAATTTCCAAACAAGAACCTGAAGAAGAATTACTGTCGTAACCCCGATAGGGAG
  CTGCGGCCTTGGTGTTTCACCACCGACCCCAACAAGCGCTGGGAACTTTGTGACATCCCC
  CGCTGCACAACACCTCCACCATCTTCTGGTCCCACCTACCAGTGTCTGAAGGGAACAGGT
  GAAAACTATCGCGGGAATGTGGCTGTTACCGTGTCCGGGCACACCTGTCAGCACTGGAGT
  GCACAGACCCCTCACACACATAACAGGACACCAGAAAACTTCCCCTGCAAAAATTTGGAT
  GAAAACTACTGCCGCAATCCTGACGGAAAAAGGGCCCCATGGTGCCATACAACCAACAGC
  CAAGTGCGGTGGGAGTACTGTAAGATACCGTCCTGTGACTCCTCCCCAGTATCCACGGAA
  CAATTGGCTCCCACAGCACCACCTGAGCTAACCCCTGTGGTCCAGGACTGCTACCATGGT
  GATGGACAGAGCTACCGAGGCACATCCTCCACCACCACCACAGGAAAGAAGTGTCAGTCT
  TGGTCATCTATGACACCACACCGGCACCAGAAGACCCCAGAAAACTACCCAAATGCTGGC
  CTGACAATGAACTACTGCAGGAATCCAGATGCCGATAAAGGCCCCTGGTGTTTTACCACA
  GACCCCAGCGTCAGGTGGGAGTACTGCAACCTGAAAAAATGCTCAGGAACAGAAGCGAGT
  GTTGTAGCACCTCCGCCTGTTGTCCTGCTTCCAGATGTAGAGACTCCTTCCGAAGAAGAC
  TGTATGTTTGGGAATGGGAAAGGATACCGAGGCAAGAGGGCGACCACTGTTACTGGGACG
  CCATGCCAGGACTGGGCTGCCCAGGAGCCCCATAGACACAGCATTTTCACTCCAGAGACA
  AATCCACGGGCGGGTCTGGAAAAAAATTACTGCCGTAACCCTGATGGTGATGTAGGTGGT
  CCCTGGTGCTACACGACAAATCCAAGAAAACTTTACGACTACTGTGATGTCCCTCAGTGT
  GCGGCCCCTTCATTTGATTGTGGGAAGCCTCAAGTGGAGCCGAAGAAATGTCCTGGAAGG
  GTTGTAGGGGGGTGTGTGGCCCACCCACATTCCTGGCCCTGGCAAGTCAGTCTTAGAACA
  AGGTTTGGAATGCACTTCTGTGGAGGCACCTTGATATCCCCAGAGTGGGTGTTGACTGCT
  GCCCACTGCTTGGAGAAGTCCCCAAGGCCTTCATCCTACAAGGTCATCCTGGGTGCACAC
  CAAGAAGTGAATCTCGAACCGCATGTTCAGGAAATAGAAGTGTCTAGGCTGTTCTTGGAG
  CCCACACGAAAAGATATTGCCTTGCTAAAGCTAAGCAGTCCTGCCGTCATCACTGACAAA
  GTAATCCCAGCTTGTCTGCCATCCCCAAATTATGTGGTCGCTGACCGGACCGAATGTTTC
  ATCACTGGCTGGGGAGAAACCCAAGGTACTTTTGGAGCTGGCCTTCTCAAGGAAGCCCAG
  CTCCCTGTGATTGAGAATAAAGTGTGCAATCGCTATGAGTTTCTGAATGGAAGAGTCCAA
  TCCACCGAACTCTGTGCTGGGCATTTGGCCGGAGGCACTGACAGTTGCCAGGGTGACAGT
  GGAGGGCCTCTGGTTTGCTTCGAGAAGGACAAATACATTTTACAAGGAGTCACTTCTTGG
  GGTCTTGGCTGTGCACGCCCCAATAAGCCTGGTGTCTATGTTCGTGTTTCAAGGTTTGTT
  ACTTGGATTGAGGGAGTGATGAGAAATAATTAA
  
  Clone variation with respect to NM_000301.3
  2286 t=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000301 unedited
  NGGGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGAATCATT
  AACTTAATTTGACTATCTGGTTTGTGGATGCGTTTACTCTCATGTAAGTCAACAACATCC
  TGGGATTGGGACCCACTTTCTGGGCACTGCTGGCCAGTCCCAAAATGGAACATAAGGAAG
  TGGTTCTTCTACTTCTTTTATTTCTGAAATCAGGTCAAGGAGAGCCTCTGGATGACTATG
  TGAATACCCAGGGGGCTTCACTGTTCAGTGTCACTAAGAAGCAGCTGGGAGCAGGAAGTA
  TAGAAGAATGTGCAGCAAAATGTGAGGAGGACGAAGAATTCACCTGCAGGGCATTCCAAT
  ATCACAGTAAAGAGCAACAATGTGTGATAATGGCTGAAAACAGGAAGTCCTCCATAATCA
  TTAGGATGAGAGATGTAGTTTTATTTGAAAAGAAAGTGTATCTCTCAGAGTGCAAGACTG
  GGAATGGAAAGAACTACAGAGGGACGATGTCCAAAACAAAAAATGGCATCACCTGTCAAA
  AATGGAGTTCCACTTCTCCCCACAGACCTAGATTCTCACCTGCTACACACCCCTCAGAGG
  GACTGGAGGAGAACTACTGCAGGAATCCAGACAACGATCCGCAGGGGCCCTGGTGCTATA
  CTACTGATCCAGAAAAGAGATATGACTACTGCGACATTCTTGAGTGTGAAGAGGAATGTA
  TGCATTGCAGTGGAGAAAACTATGACGNGCAAATTTCCAAGACCATGTCTGGACTGGGAT
  GCCAGGCCTGNGACTCTCANAGCCACACGCTCATGGATACATTNCTTNNCAATTTNNCAA
  CAGAACCTGAGAGAANTACTGTCGTAACCCGATAGGAGCTGCGNCTTTGGTGTTTACACC
  GACCCACAGCGCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000301 unedited
  NNCCTTACTTGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTCAA
  AGTTAAGTACAGAGTTTATTTTTAACAAATGTCATAGCTATGTCACCTTACTACAGAATC
  CAGCAGTCAGAAAATAACACAAAAAATGCCGAGGTTTGGCGTAGCTGGTAGCTGGGGACA
  GTGTCTTCGTTTGATTACTGCCAGTTATTTCCAGCATGCTAAATCCCTACCCACGTCCCA
  GCCTCTAGGTGAGTCAGTGCGTCACTCTGTCTCCCGTCCAATTAATTATTTCTCATCACT
  CCCTCAATCCAAGTAACAAACCTTGAAACACGAACATAGACACCAGGCTTATTGGGGCGT
  GCACAGCCAAGACCCCAAGAAGTGACTCCTTGTAAAATGTATTTGTCCTTCTCGAAGCAA
  ACCAGAGGCCCTCCACTGTCACCCTGGCAACTGTCAGTGCCTCCGGCCAAATGCCCAGCA
  CAGAGTTCGGTGGATTGGACTCTTCCATTCAGAAACTCATAGCGATTGCACACTTTATTC
  TCAATCACAGGGAGCTGGGCTTCCTTGAGAAGGCCAGCTCCAAAAGTACCTTGGGTTTCT
  CCCCAGCCAGTGATGAAACATTCGGTCCGGTCAGCGACCACATAATTTGGGGATGGCAGA
  CAAGCTGGGATTACTTTGTCAGTGATGACGGCAGGACTGCTTAGCTTTAGCAAGGCAATA
  TCTTTTCGTGTGGGCTCCAAGACAGCCTAGACACTTCTATNTNCTGAACATGCGGGTCGA
  GATTCACTTCTTGGTGTGCACCCAGNATGACCTTGTANGATGAAAGGCCTTNGGGACTCT
  CCAAGCAGTGGGCAGCAGTCAACACCCACTCTGGNGATATCAAGTGCCTNCACAGAGTGC
  ATTCCAAACTTTGTNCTAGACTGACTTGCAGGNCCAGAATGTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000301
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000301.1, NP_000292.1
• RefSeq Size: 2732 bp
• RefSeq ORF: 2433 bp
• Locus ID: 5340
• Cytogenetics: 6q26
• Domains: KR, Tryp_SPc, PAN, PAN_AP
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Neuroactive ligand-receptor interaction
• Gene Summary: 'The plasminogen protein encoded by this gene is a serine protease that circulates in blood plasma as an inactive zymogen and is converted to the active protease, plasmin, by several plasminogen activators such as tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). The conversion of plasminogen to plasmin involves the cleavage of the peptide bond between Arg-561 and Val-562. Plasmin cleavage also releases the angiostatin protein which inhibits angiogenesis. Plasmin degrades many blood plasma proteins, including fibrin-containing blood clots. As a serine protease, plasmin cleaves many products in addition to fibrin such as fibronectin, thrombospondin, laminin, and von Willebrand factor. Plasmin is inactivated by proteins such as alpha-2-macroglobulin and alpha-2-antiplasmin in addition to inhibitors of the various plasminogen activators. Plasminogen also interacts with plasminogen receptors which results in the retention of plasmin on cell surfaces and in plasmin-induced cell signaling. The localization of plasminogen on cell surfaces plays a role in the degradation of extracellular matrices, cell migration, inflamation, wound healing, oncogenesis, metastasis, myogenesis, muscle regeneration, neurite outgrowth, and fibrinolysis. This protein may also play a role in acute respiratory distress syndrome (ARDS) which, in part, is caused by enhanced clot formation and the suppression of fibrinolysis. Compared to other mammals, the cluster of plasminogen-like genes to which this gene belongs has been rearranged in catarrhine primates. [provided by RefSeq, May 2020]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.