RPGR (NM_000328) Human Untagged Clone

CAT#: SC119976

RPGR (untagged)-Human retinitis pigmentosa GTPase regulator (RPGR), transcript variant A

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPGR
• Synonyms: COD1; CORDX1; CRD; orf15; PCDX; RP3; RP15; XLRP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119976 sequence for NM_000328 edited (data generated by NextGen Sequencing)
  ATGAGGGAGCCGGAAGAGCTGATGCCCGATTCGGGTGCTGTGTTTACATTTGGGAAAAGT
  AAATTTGCTGAAAATAATCCCGGTAAATTCTGGTTTAAAAATGATGTCCCTGTACATCTT
  TCATGTGGAGATGAACATTCTGCTGTTGTTACCGGAAATAATAAACTTTACATGTTTGGC
  AGTAACAACTGGGGTCAGTTAGGATTAGGATCAAAGTCAGCCATCAGCAAGCCAACATGT
  GTCAAAGCTCTAAAACCTGAAAAAGTGAAATTAGCTGCCTGTGGAAGGAACCACACCCTG
  GTGTCAACAGAAGGAGGCAATGTATATGCAACTGGTGGAAATAATGAAGGACAGTTGGGG
  CTTGGTGACACCGAAGAAAGAAACACTTTTCATGTAATTAGCTTTTTTACATCCGAGCAT
  AAGATTAAGCAGCTGTCTGCTGGATCTAATACTTCAGCTGCCCTAACTGAGGATGGAAGA
  CTTTTTATGTGGGGTGACAATTCCGAAGGGCAAATTGGTTTAAAAAATGTAAGTAATGTC
  TGTGTCCCTCAGCAAGTGACCATTGGGAAACCTGTCTCCTGGATCTCTTGTGGATATTAC
  CATTCAGCTTTTGTAACAACAGATGGTGAGCTATATGTGTTTGGAGAACCTGAGAATGGG
  AAGTTAGGTCTTCCCAATCAGCTCCTGGGCAATCACAGAACACCCCAGCTGGTGTCTGAA
  ATTCCGGAGAAGGTGATCCAAGTAGCCTGTGGTGGAGAGCATACTGTGGTTCTCACGGAG
  AATGCTGTGTATACCTTTGGGCTGGGACAATTTGGTCAGCTGGGTCTTGGCACTTTTCTT
  TTTGAAACTTCAGAACCCAAAGTCATTGAGAATATTAGGGATCAAACAATAAGTTATATT
  TCTTGTGGAGAAAATCACACAGCTTTGATAACAGATATCGGCCTTATGTATACTTTTGGA
  GATGGTCGCCACGGAAAATTAGGACTTGGACTGGAGAATTTTACCAATCACTTCATTCCT
  ACTTTGTGCTCTAATTTTTTGAGGTTTATAGTTAAATTGGTTGCTTGTGGTGGATGTCAC
  ATGGTAGTTTTTGCTGCTCCTCATCGTGGTGTGGCAAAAGAAATTGAATTCGATGAAATA
  AATGATACTTGCTTATCTGTGGCGACTTTTCTGCCGTATAGCAGTTTAACCTCAGGAAAT
  GTACTGCAGAGGACTCTATCAGCACGTATGCGGCGAAGAGAGAGGGAGAGGTCTCCAGAT
  TCTTTTTCAATGAGGAGAACACTACCTCCAATAGAAGGGACTCTTGGCCTTTCTGCTTGT
  TTTCTCCCCAATTCAGTCTTTCCACGATGTTCTGAGAGAAACCTCCAAGAGAGTGTCTTA
  TCTGAACAGGACCTCATGCAGCCAGAGGAACCAGATTATTTGCTAGATGAAATGACCAAA
  GAAGCAGAGATAGATAATTCTTCAACTGTAGAAAGCCTTGGAGAAACTACTGATATCTTA
  AACATGACACACATCATGAGCCTGAATTCCAATGAAAAGTCATTAAAATTATCACCAGTT
  CAGAAACAAAAGAAACAACAAACAATTGGGGAACTGACGCAGGATACAGCTCTTACTGAA
  AACGATGATAGTGATGAATATGAAGAAATGTCAGAAATGAAAGAAGGGAAAGCATGTAAA
  CAACATGTGTCACAAGGGATTTTCATGACGCAGCCAGCTACGACTATCGAAGCATTTTCA
  GATGAGGAAGTAGAGATCCCAGAGGAGAAGGAAGGAGCAGAGGATTCAAAAGGAAATGGA
  ATAGAGGAGCAAGAGGTAGAAGCAAATGAGGAAAATGTGAAGGTGCATGGAGGAAGAAAG
  GAGAAAACAGAGATCCTATCAGATGACCTTACAGACAAAGCAGAGGATCATGAATTTTCT
  AAAACTGAGGAACTAAAACTAGAAGATGTGGATGAGGAAATTAATGCTGAAAATGTGGAA
  AGCAAGAAGAAAACTGTGGGAGATGATGAAAGTGTTCCTACAGGTTATCACAGTAAAACA
  GAAGGAGCAGAAAGAACCAATGATGATAGCTCAGCTGAAACTATTGAAAAGAAAGAAAAA
  GCCAACCTAGAGGAACGGGCCATTTGTGAGTACAATGAAAACCCAAAAGGATACATGCTT
  GATGATGCAGATAGCAGTTCATTAGAAATCCTAGAAAACAGTGAAACAACACCAAGCAAA
  GACATGAAAAAAACAAAGAAGATTTTTCTGTTCAAAAGAGTCCCCTCAATAAATCAAAAG
  ATTGTCAAGAATAACAATGAGCCGCTCCCAGAGATAAAATCCATAGGAGACCAGATCATT
  TTAAAAAGTGATAATAAAGATGCCGACCAGAACCACATGAGTCAGAATCATCAGAATATC
  CCACCAACAAATACAGAGAGAAGATCAAAATCCTGTACAATACTATAA
  
  Clone variation with respect to NM_000328.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000328 unedited
  TGTAGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTTTGTTGACT
  TCCGGGAGCGCGGTTGTAGTTGATCTCCGGAGTTTCGCCATGCGGAACTTGGGGGCTTTC
  GCGGCCCGCGTCGGTGCGGAGTAGCTGCTTTAGCCCCGACCAAACCGTCCTCTACAGCCT
  CCTGGCCCCGGCGCAGGCTGCCCGTACTGCCCGTGGCATGAGGGAGCCGGAAGAGCTGAT
  GCCCGATTCGGGTGCTGTGTTTACATTTGGGAAAAGTAAATTTGCTGAAAATAATCCCGG
  TAAATTCTGGTTTAAAAATGATGTCCCTGTACATCTTTCATGTGGAGATGAACATTCTGC
  TGTTGTTACCGGAAATAATAAACTTTACATGTTTGGCAGTAACAACTGGGGTCAGTTAGG
  ATTAGGATCAAAGTCAGCCATCAGCAAGCCAACATGTGTCAAAGCTCTAAAACCTGAAAA
  AGTGAAATTAGCTGCCTGTGGAAGGAACCACACCCTGGTGTCAACAGAAGGAGGCAATGT
  ATATGCAACTGGTGGAAATAATGAAGGACAGCTGGGGCTTGGTGACACCGAAGAAAGACC
  ACTTTTTATGTAATTAGCTCTTTTACATCCGAGCATAAGATTAAGCAGCTGTCTGCTGGA
  TCTAATACTTCAGCTGCCCTAACTGATGATGGGAGACTTTTTATGTGGGGTGAACATTCC
  CAAAGGCCAATTGCTTTAAAAAATGTAAGTCATGTCTTGTGTCCCTTACCAGGGACCATT
  GGGAAACCTGTCTCCTGNATTCCTTGGGGATATTACCATTCACTTTTGTAACACAGATGG
  TGAGCTTTTGTGTTTGAAGAACTGAGAATGGAAATTAGGTCTTCCAATCACTTCCTGGCA
  TCCCAAAACCCCACCTGGGGTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000328 unedited
  TGGCCGCGGCCGCAATCTAGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTAACAGGGGAAACTGACCAGATATAGTTTATTAAAATATGTTCATTATAAACAA
  TTTTACCTCAGTTTTGTAAAAATTGTAAATACATCAAAAACTGTTACAGGGTCGAAAAAT
  TGCAAATATTGCCTTATAAAATTATAAAGAAGTAAAATTGTTTAGTAAAAACTAGGAAAT
  ATTTTATTTCAATTTTCCACATATAAAAATATATTTTTTATTGCAATACTGAACAGTTAA
  GGCCACAACACTTTAGGGAGAATTTGAGATACACATATTTTGAAATGACTTGTTAAAAAT
  ATTAGCATAAATAAATATCAAAACTGGTCACACTTATAGAAGCTGAATAAAACATATTAA
  GTCTTATATCTTTTAAAGACTACTATCAAAAACTTTACTTCATAAGTTATAACATTTTTC
  AAGTATACATTACAATACACTTGGGGACTGTGAAAACATAAATATATATTTATAGTATTG
  GACAGGATTTTGATCTTCTCTCTGTATTTGGGGGGGGGATATTCTGATGATTCTGACTCA
  TGTGGTTCTGGTCGGCATCTTTATTATCACTTTTTAAAATGATCTGGCCTCCTATGGATT
  TTATCTCTGGGAGCGGCTCATTGTTATTCTTGACAATCTTTTGATTTATTGAGGGGACTC
  TTTTGAACAAAAAAATCTCTTTGTTTTTTCATGCCTTTGCTTGGGGGTGTTCACTGTTTC
  CTAGATTTCTAATGACTGCTATTTGATATCAACATGTTCCCTTGGGTTTTATGGACTCAC
  AATGGCCGTCCTCTAGTGGCTTTCCTTTTTTAAAAGTTAAGTGACTTTTCATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000328
• Insert Size: 3240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000328.2, NP_000319.1
• RefSeq Size: 3082 bp
• RefSeq ORF: 2448 bp
• Locus ID: 6103
• Cytogenetics: Xp11.4
• Domains: RCC1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. [provided by RefSeq, Dec 2008]'
  Transcript Variant: This variant (A) uses an alternate splice site and contains multiple alternative exons in the 3' coding region, compared to variant C. The resulting isoform (A, also referred to as isoform 1) is shorter and has a distinct C-terminus, compared to isoform C.