XPB (ERCC3) (NM_000122) Human Untagged Clone

CAT#: SC120091

ERCC3 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERCC3
• Synonyms: BTF2; GTF2H; RAD25; TFIIH; TTD2; XPB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000122, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAAAAGAGACCGAGCGGACCGCGACAAGAAGAAATCCAGGAAGCGGCACTATGAGGATGAAGAGG
  ATGATGAAGAGGACGCCCCGGGGAACGACCCTCAGGAAGCGGTTCCCTCGGCGGCGGGGAAGCAGGTGGA
  TGAGTCAGGCACCAAAGTGGATGAATATGGAGCCAAGGACTACAGGCTGCAAATGCCGCTGAAGGACGAC
  CACACCTCCAGGCCCCTCTGGGTGGCTCCCGATGGCCATATCTTCTTGGAAGCCTTCTCTCCAGTTTACA
  AATATGCCCAAGACTTCTTGGTGGCTATTGCAGAGCCAGTGTGCCGACCAACCCATGTGCATGAGTACAA
  ACTAACTGCCTACTCCTTGTATGCAGCTGTCAGCGTTGGGCTGCAAACCAGTGACATCACCGAGTACCTC
  AGGAAGCTCAGCAAGACTGGAGTCCCTGATGGAATTATGCAGTTTATTAAGTTGTGTACTGTCAGCTATG
  GAAAAGTCAAGCTGGTCTTGAAGCACAACAGATACTTCGTTGAAAGTTGCCACCCTGATGTAATCCAGCA
  TCTTCTCCAGGACCCCGTGATCCGAGAATGCCGCTTAAGAAACTCTGAAGGGGAGGCCACTGAGCTCATC
  ACAGAGACTTTCACAAGCAAATCTGCCATTTCTAAGACTGCTGAAAGCAGTGGTGGGCCCTCCACTTCCC
  GAGTGACAGATCCACAGGGTAAATCTGACATCCCCATGGACCTGTTTGACTTCTATGAGCAAATGGACAA
  GGATGAAGAAGAAGAAGAAGAGACACAGACAGTGTCTTTTGAAGTCAAGCAGGAAATGATTGAGGAACTC
  CAGAAACGTTGCATCCACCTGGAGTACCCTCTGTTGGCAGAATATGACTTCCGGAATGATTCTGTCAACC
  CTGATATCAACATTGACCTAAAGCCCACAGCTGTCCTCAGACCCTATCAGGAGAAGAGCTTGCGAAAGAT
  GTTTGGAAACGGGCGTGCACGTTCGGGGGTCATTGTTCTTCCCTGCGGTGCTGGAAAGTCCCTGGTTGGT
  GTGACTGCTGCATGCACTGTCAGAAAACGCTGTCTGGTGCTGGGCAACTCAGCTGTTTCTGTGGAGCAGT
  GGAAAGCCCAGTTCAAGATGTGGTCCACCATTGACGACAGCCAGATCTGCCGGTTCACCTCCGATGCCAA
  GGACAAGCCCATCGGCTGCTCCGTTGCCATTAGCACCTACTCCATGCTGGGCCACACCACCAAAAGGTCC
  TGGGAGGCCGAGCGAGTCATGGAGTGGCTCAAGACCCAGGAGTGGGGCCTCATGATCCTGGATGAAGTGC
  ACACCATACCAGCCAAGATGTTCCGAAGGGTGCTCACCATCGTGCAGGCCCACTGTAAGCTGGGTTTGAC
  TGCGACCCTCGTCCGCGAAGATGACAAAATTGTGGATTTAAATTTTCTGATTGGGCCTAAGCTCTACGAA
  GCCAACTGGATGGAGCTGCAGAATAATGGCTACATCGCCAAAGTCCAGTGTGCTGAGGTCTGGTGCCCTA
  TGTCTCCTGAATTTTACCGGGAATATGTGGCAATCAAAACCAAGAAACGAATCTTGCTGTACACCATGAA
  CCCCAACAAATTTAGAGCTTGCCAGTTTCTGATCAAGTTTCATGAAAGGAGGAATGACAAGATTATTGTC
  TTTGCTGACAATGTGTTTGCCCTAAAGGAATATGCCATTCGACTGAACAAACCCTATATCTACGGACCTA
  CGTCTCAGGGGGAAAGGATGCAAATTCTCCAGAATTTCAAGCACAACCCCAAAATTAACACCATCTTCAT
  ATCCAAGGTAGGTGACACTTCGTTTGATCTGCCGGAAGCAAATGTCCTCATTCAGATCTCATCCCATGGT
  GGCTCCAGGCGTCAGGAAGCCCAAAGGCTAGGGCGGGTGCTTCGAGCTAAAAAAGGGATGGTTGCAGAAG
  AGTACAATGCCTTTTTCTACTCACTGGTATCCCAGGACACACAGGAAATGGCTTACTCAACCAAGCGGCA
  GAGATTCTTGGTAGATCAAGGTTATAGCTTCAAGGTGATCACGAAACTCGCTGGCATGGAGGAGGAAGAC
  TTGGCGTTTTCGACAAAAGAAGAGCAACAGCAGCTCTTACAGAAAGTCCTGGCAGCCACTGACCTGGATG
  CCGAGGAGGAGGTGGTGGCTGGGGAATTTGGCTCCAGATCCAGCCAGGCATCTCGGCGCTTTGGCACCAT
  GAGTTCTATGTCTGGGGCCGACGACACTGTGTACATGGAGTACCACTCATCGCGGAGCAAGGCGCCCAGC
  AAACATGTACACCCGCTCTTCAAGCGCTTTAGGAAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000122 unedited
  ATATCCAGATTTGTATACGACTCATATGGGCGGCCGCGCAATTCGCACGAGGGCGGGCCT
  GTGGGAGCGGGGTCATCTTCTCTCTGCTGCTGTAGCTGCCATGGGCAAAAGAGACCGAGC
  GGACCTGCGACAAGTAAGAAATCCAGGAAGCGGCACTATGAGGATGAAGAGGATGATGAA
  GAGGACGCCCCGGGGAACGACCCTCAGGAAGCGGTTCCCTCGGCGGCGGGGAAGCAGGTG
  GATGAGTCAGGCACCAAAGTGGATGAATATGGAGCCAAGGACTACAGGCTGCAAATGCCG
  CTGAAGGACGACCACACCTCCAGGCCCCTCTGGGTGGCTCCCGATGGCCATATCTTCTTG
  GAAGCCTTCTCTCCAGTTTACAAATATGCCCAAGACTTCTTGGTGGCTATTGCAGAGCCA
  GTGTGCCGACCAACCCATGTGCATGAGTACAAACTAACTGCCTACTCCTTGTATGCAGCT
  GTCAGCGTTGGGCTGCAAACCAGTGACATCACCGAGTACCTCAGGAAGCTCAGCAAGACT
  GGAGTCCCTGATGGAATTATGCAGTTTATTAAGTTGTGTACTGTCAGCTATGGAAAAGTC
  AAGCTGGTCTTGAAGCACAACAGGTAAGAGATTCCATGACAGGCCTGTCCCCAGGCACTG
  TCACTTTCTGCGCACCAGCAGGAATAAAGGGATATGGGCAGGACCTGATGTTTATGCTGT
  CTTTTCTGTGAGCCCAGATAAGAGCAGAGAAACAGAGATGTGCCCCGACTGGAACTCATC
  CCTGCCCACTGTATTCCTCTAGCATGGAGCTGGCTCAGCCCCTGCCTACTTGCCTCAGGT
  CCCACCCTCTATGTTAAATCATGCCGCAGCTCATGCTCAGANTTGGCGAAGGGTGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000122 unedited
  NTTTTACTATGGACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTCGGGGTCAAGGGGCCATTCTGTTTATTCAAAACGGGAA
  CATAAATACCCCTTAAATATTAACATTTTTTATATACAGAAATGACCCCACTCCCCAAAA
  AGTTTGAAAAAATATTGGCTCCTCCTCCTTTATAAAACCCTAAATGACCTATGAAGGCAC
  AGCCAAGCCCTTGATGCATTTTCCTAAGCACAATTTGGCCAACGCTGGAGGGAAGGTCAA
  AGAGGGGGAAGGAAAATGTTATGCTGAAAATCCCTTTCCAACAAGGGGGCCAAGCGCCGG
  TCTTGAACAAAGTACCCTGCCTAAGCATCATTTCCTAAAGCGCTTGAAAAGCGGGTGTAC
  ATGTTTGCTGGGCGCCTTGCTCCGCGATGAGGGGTACTCCATGTACACAGGGTCGTCGGC
  CCCAAACATAAAACTCATGGGGCCAAAGCGCCGAAATGCCTGGCTGGATCTGGAGCCAAA
  TTCCCCAGCCACCACCTCCTCCTGGGAATCCAGGTCAGGGGCTGCCAGGACTTTCTGTAA
  AAGCTGCTGTTGCTCTTCTTTTGTCGAAAACGCCAAGTTTTCCTCCTCCATGCCAGCGAG
  TTTCGTGATCACCTTGAAGCTATAACCTTGATCTACCAAGAATCTCTGCCGCTTGGTTGA
  GTAAGCCATTTCCTGTGTGTCCTGGGATACCAGGGAGTAGAAAAAGGCATTGTACTCTTC
  TGCAACCATCCCTTTTTTAGCTCGAAGCACCCGCCCTAACCTTTGGGCTTCCTGACGCCT
  GGAGCCCCATGGGATGAGATCTGAATGAGACATTTGCTCCGGCAATCAAACGAGTGCACC
  TACCTTGGATTGAAAATGGNGTATTTTGGGTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000122
• Insert Size: 3260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000122.1, NP_000113.1
• RefSeq Size: 2751 bp
• RefSeq ORF: 2349 bp
• Locus ID: 2071
• Cytogenetics: 2q14.3
• Domains: DEAD, helicase_C
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair
• Gene Summary: 'This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]'
  Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (a).