MYL12B (NM_033546) Human Untagged Clone

CAT#: SC120113

MYL12B (untagged)-Human myosin, light chain 12B, regulatory (MYL12B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYL12B
• Synonyms: MLC-B; MRLC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120113 sequence for NM_033546 edited (data generated by NextGen Sequencing)
  ATGTCGAGCAAAAAGGCAAAGACCAAGACCACCAAGAAGCGCCCTCAGCGTGCAACATCC
  AATGTGTTTGCYATGTTTGACCAGTCACAGATTCAGGAGTTCAAAGAGGCCTTCAACATG
  ATTGATCAGAACAGAGATGGYTTCATCGACAAGGAAGATTTGCATGATATGCTTGCTTCT
  CTAGGGAAGAATCCCACTGATGCATACCTTGATGCCATGATGAATGAGGCYCCAGGGCCC
  ATCAATTTCACCATGTTCCTSACCATGTTTGGTGAGAAGTTAAATGGCACAGATCCTGAA
  GATGTCATCAGAAAYGCCTTTGCTTGCTTTGATGAAGAAGCAACWGGCACCATTCAGGAA
  GATTACCTAAGAGAGCTGCTGACAACCATGGGGGATCGGTTTACAGATGAGGAAGTGGAT
  GAGCTGTACAGAGAAGCACCTATTGAYAAAAAGGGGAATTTCAATTACATCGAGTTCACA
  CGCATCCTGAAACATGGAGCCAAAGACAAAGATGACTGA
  
  Clone variation with respect to NM_033546.3
  72 c=>y;141 c=>y;231 c=>y;261 g=>s;315 c=>y;345 a=>w;447 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033546 unedited
  TGTATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGGTGTTTGGCGTCGGAA
  TTAAACAACCACCATGTCGAGCAAAAAGGCAAAGACCAAGACCACCAAGAAGCGCCCTCA
  GCGTGCAACATCCAATGTGTTTGCCATGTTTGACCAGTCACAGATTCAGGAGTTCAAAGA
  GGCCTTCAACATGATTGATCAGAACAGAGATGGCTTCATCGACAAGGAAGATTTGCATGA
  TATGCTTGCTTCTCTAGGGAAGAATCCCACTGATGCATACCTTGATGCCATGATGAATGA
  GGCCCCAGGGCCCATCAATTTCACCATGTTCCTGACCATGTTTGGTGAGAAGTTAAATGG
  CACAGATCCTGAAGATGTCATCAGAAACGCCTTTGCTTGCTTTGATGAAGAAGCAACAGG
  CACCATTCAGGAAGATTACCTAAGAGAGCTGCTGACAACCATGGGGGATCGGTTTACAGA
  TGAGGAAGTGGATGAGCTGTACAGAGAAGCACCTATTGACAAAAAGGGGAATTTCAATTA
  CATCGAGTTCACACGCATCCTGAAACATGGAGCCAAAGACAAAGATGACTGAAAGAACTT
  TAGCTAAAATCTTCCAGTTACATTGTCTTACTCTCTTTTACTTCTCAGACACTTCCCCCA
  CCCTCATAGAACCTGTTGCATGCAACTTAGTTTCACAGCTTTGCCTCTTCTTTTTGATGT
  ATTTATTCCAGACCTTTCTGCCACTTAGCACTTGTATAATCAGACTGGAAATGGGGATGA
  GGGTGTAAATTGTATTGAAAAAGATCGCGAATAAAAATCAACAAATGTGAAAGCCCAGAA
  NNAAAAAAAAAAAAACTCGACTCTAGATTGCNNGCCGCGTCATAGCTGTTTCCTTGACAG
  ATCCCCGGNTGGCATCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033546 unedited
  GCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTGGGCTTTCCATTTGTTG
  ATTTTTATTCGCGATCTTTTTCAATACAATTTACACCCTCATCCCCATTTCCAGTCTGAT
  TATACAAGTGCTAAGTGGCAGAAAGGTCTGGAATAAATACATCAAAAAGAAGAGGCAAAG
  CTGTGAAACTAAGTTGCATGCAACAGGTTCTATGAGGGTGGGGGAAGTGTCTGAGAAGTA
  AAAGAGAGTAAGACAATGTAACTGGAAGATTTTAGCTAAAGTTCTTTCAGTCATCTTTGT
  CTTTGGCTCCATGTTTCAGGATGCGTGTGAACTCGATGTAATTGAAATTCCCCTTTTTGT
  CAATAGGTGCTTCTCTGTACAGCTCATCCACTTCCTCATCTGTAAACCGATCCCCCATGG
  TTGTCAGCAGCTCTCTTAGGTAATCTTCCTGAATGGTGCCTGTTGCTTCTTCATCAAAGC
  AAGCAAAGGCGTTTCTGATGACATCTTCAGGATCTGTGCCATTTAACTTCTCACCAAACA
  TGGTCAGGAACATGGTGAAATTGATGGGCCCTGGGGCCTCATTCATCATGGCATCAAGGT
  ATGCATCAGTGGGATTCTTCCCTAGAGAAGCAAGCATATCATGCAAATCTTCCTTGTCGA
  TGAAGCCATCTCTGTTCTGATCAATCATGTTGAAGGCCTCTTTGAACTCCTGAATCTGTG
  ACTGGTCAAACATGGCAAACACATTGGATGTTGCACGCTGAGGGCGCTTCTTGGAGTCTT
  GGTCTTTGCCTTTNTGCTCGAATGGGGGNNTGTTAATTCCGACCCAAACACCCCTCGTGC
  CGAATTCGCGGCCGCCTATAGTGAGTCGTATACAAAATCTGACGGTCACTAACGAGCCTG
  CTATATGACCNCCACGTACAGCCTACGCCATTTGGTNACGGGGCGGGTATACAATTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033546
• ORF Size: 519 bp
• Insert Size: 820
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_033546.2, NP_291024.1
• RefSeq Size: 878
• RefSeq ORF: 519
• Locus ID: 103910
• Domains: EFh
• Protein Pathways: Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction
• Gene Summary: The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 all encode the same protein.