DAZL (BC027595) Human Untagged Clone

CAT#: SC122232

DAZL (untagged)-Human deleted in azoospermia-like (cDNA clone MGC:26406 IMAGE:4829381), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAZL
• Synonyms: DAZH; DAZL1; DAZLA; deleted in azoospermia-like; deleted in azoospermia-like autosomal; germline specific RNA binding protein; MGC26406; spermatogenesis gene on the Y-like autosomal; SPGYLA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC027595 edited
  ATTGGCTGAGGCCGATACCACGCGCCCCGATACCCGGCGCAGGAGCCACCTCCCTGAGCC
  CCGCGGACCACGCCTCAGTCCGCCTGCGCTCCTCAGCCTGACGGTCCGCCTTTCGGGGCT
  CCTCAGCCTTGTCACCCGCTCTTGGTTTTCCTTTTCTCTTCATCTTTGGCTCCTTTGACC
  ACTCGAAGCCGCGCAGCGGGTTCCAGTGGACCTCACAGCAGCCCCAGAAGTGGTGCGCCA
  AGCACAGCCTCTGCTCCTCCTCGAGCCGGTCGGGAACTGCTGCCTGCCGCCATCATGTCT
  ACTGCAAATCCTGAAACTCCAAACTCAACCATCTCCAGAGAGGCCAGCACCCAGTCCTCA
  TCAGCTGCAACCAGCCAAGGCTATATTTTACCAGAAGGCAAAATCATGCCAAACACTGTT
  TTTGTTGGAGGAATTGATGTTAGGATGGATGAAACTGAGATTAGAAGCTTCTTTGCTAGA
  TATGGTTCAGTGAAAGAAGTGAAGATAATCACTGATCGAACTGGTGTGTCCAAAGGCTAT
  GGATTTGTTTCATTTTTTAATGACGTGGATGTGCAGAAGATAGTAGAATCACAGATAAAT
  TTCCATGGTAAAAAGCTGAAGCTGGGCCCTGCAATCAGGAAACAAAATTTATGTGCTTAT
  CATGTGCAGCCACGTCCTTTGGTTTTTAATCATCCTCCTCCACCACAGTTTCAGAATGTC
  TGGACTAATCCAAACACTGAAACTTATATGCAGCCCACAACCACGATGAATCCTATAACT
  CAGTATGTTCAGGCATATCCTACTTACCCAAATTCACCAGTTCAGGTCATCACTGGATAT
  CAGTTGCCTGTATATAATTATCAGATGCCACCACAGTGGCCTGTTGGGGAGCAAAGGAGC
  TATGTTGTACCTCCGGCTTATTCAGCTGTTAACTACCACTGTAATGAAGTTGATCCAGGA
  GCTGAAGTTGTGCCAAATGAATGTTCAGTTCATGAAGCTACTCCACCCTCTGGAAATGGC
  CCACAAAAGAAATCTGTGGACCGAAGCATACAAACGGTGGTATCTTGTCTGTTTAATCCA
  GAGAACAGACTGAGAAACTCTGTTGTTACTCAAGATGACTACTTCAAGGATAAAAGAGTG
  CATCACTTTAGAAGAAGTCGGGCAATGCTTAAATCTGTTTGATCCTCCTGGCTTATCTAG
  TTACATGGGAAGTTGCTGGTTTTGAATATTAAGCTAAAAGGTTTCCACTATTATAGAAAT
  TCTGAATTTTGGTAAATCACACTCAAACTTTGTGTATAAGTTGTATTATTAGACTCTCTA
  GTTTTATCTTAAACTGTTCTTCATTAGATGTTTATTTAGAAACTGGTTCTGTGTTGAAAT
  ATAGTTGAAAGTAAAAAAATAATTGAGACTGAAAGAAACTAAGATTTATCTGCAAGGATT
  TTTTAAAAATTGGCATTTTAAGTGTTTAAAAGCAAATACTGATTTTCAAAAAAATGTTTT
  TAAAAACCTATTTTGAAAGGTCAGAATTTTGTTGGTCTGAATACAAACATTTCACTTCTC
  CAACAAGTACCTGTGAACAGTACAGTATTTACAGTATTGAGCTTTGCATTTATGATTTCT
  CCAGAAATTTACCACAAAAGCAAAATTTTTAAAACTGCATTTTTAATCAGTGGAACTCAA
  TATATAGTTAGCTTTATTGAAGTCTTCTTATCTAAACCCAGCAAAACAGATTCAAAGCAA
  ACAGTCCAATCAGTGGGTCATATGTTTATTCAAAATATTTTATCTTTTAGCTAGAATCCA
  CACATATATATCCTATTTGATTAGGGTAGTAATTAGGATAACTAAAATTCTGGGCCTAAT
  TTTTTAAAGAATCCAAGACAAACTAAACTTTACTAGGTACATAAGCTTCTCAATGAGTCA
  CCATTCTTCTTTTTTGTAAAAACTTTTTTCTTTGAAATGCTAAACTTGGCTGTATGTCAA
  ATTGTGCAAAATATTGGTATTAAAGAATGCTGCAACTTTAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC027595 unedited
  GTTTGGGATTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACAT
  TATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGATTGG
  CTGAGGCCGATACCACGCGCCCCGATACCCGGCGCATGAGCCACCTCCCTGAGCCCCGCG
  GACCACGCCTCAGTCCGCCTGCGCTCCTCAGCCTGACGGTCCGCCTTTCGGGGCTCCTCA
  GCCTTGTCACCCGCTCTTGGTTTTCCTTTTCTCTTCATCTTTGGCTCCTTTGACCACTCG
  AAGCCGCGCAGCGGGTTCCAGTGGACCTCACAGCAGCCCCAGAAGTGGTGCGCCAAGCAC
  AGCCTCTGCTCCTCCTCGAGCCGGTCGGGAACTGCTGCCTGCCGCCATCATGTCTACTGC
  AAATCCTGANACTCCAAACTCAACCATCTCCAGAGAGGCCAGCACCCAGTCCTCATCAGC
  TGCAACCAGCCAAGGCTATATTTTACCAGAAGGCATAATCATGCCAAACACTGTTTTTGT
  TGGAGGAATTGATGTTAGGATGGATGAAACTGAGATTAGAAGCTTCTTTGCTAGATATGG
  TTCAGTGAAAGAAGTGAAGATAATCACTGATCGAACTGGTGTGTCCAAAGGCTATGGATT
  TGTTTCATTTTTTAATGACGTGGATGTGCAGAAGATAGTAGAATCACAGATAAATTTCCA
  TGGTAAAAAGCTGAAGCTGGGCCCCTGCATCAGGANACANAATTTATGTGCTTATCATGT
  GCAGCCACGTCCTTTGGNTTTTAATCATCCTCCTNCACCACAGTTTNCAGATGTCTGGAC
  TAATCCAACACTGAANCTTATATGCAGCCCCACACCACGATGAATCCTATNACTCAGTAT
  GTTCAGGCATATCCTACTTTACCAATTNCACAGTTCAGG
  
• Restriction Sites: Please inquire
• ACCN: BC027595
• Insert Size: 2038 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027595.2, AAH27595.1
• RefSeq Size: 2033 bp
• Locus ID: 1618
• Cytogenetics: 3p24.3
• Gene Summary: 'The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'