GSTA3 (NM_000847) Human Untagged Clone

CAT#: SC122577

GSTA3 (untagged)-Human glutathione S-transferase alpha 3 (GSTA3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GSTA3
• Synonyms: GSTA3-3; GTA3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000847 edited
  GGTGGCCTTGAGAAGCTGAGCGGAGACCGGCTAGACTTTACTCAAAGAAACCAAGAGACT
  GTTACCATGGCAGGGAAGCCCAAGCTTCACTACTTCAATGGACGGGGCAGAATGGAGCCC
  ATCCGGTGGCTCTTGGCTGCAGCTGGAGTGGAGTTTGAAGAGAAATTTATAGGATCTGCA
  GAAGATTTGGGAAAGTTAAGAAATGATGGGAGTTTGATGTTCCAGCAAGTACCAATGGTT
  GAGATTGATGGGATTAAGTTGGTACAGACCAGAGCCATTCTCAACTACATTGCCAGCAAA
  TACAACCTCTACGGGAAAGACATAAAGGAGAGAGCCCTAATTGATATGTATACAGAAGGT
  ATGGCAGATTTGAATGAAATGATCCTTCTTCTGCCCTTATGTCGACCTGAGGAAAAAGAT
  GCCAAGATTGCCTTGATCAAAGAGAAAACAAAAAGTCGCTATTTCCCTGCCTTCGAAAAA
  GTGTTACAGAGCCATGGACAAGACTACCTTGTTGGCAACAAGCTGAGCCGGGCTGACATT
  AGCCTGGTGGAACTTCTCTACTATGTGGAAGAGCTTGACTCCAGCCTTATCTCCAACTTC
  CCTCTGCTGAAGGCCCTGAAAACCAGAATCAGCAACCTGCCCACGGTGAAGAAGTTTCTA
  CAGCCTGGCAGCCCAAGGAAGCCTCCCGCAGATGCAAAAGCTTTAGAAGAAGCCAGAAAG
  ATTTTCAGGTTTTAATAAAGCAGCCATGGAGGCTAAGAACATGCAAGACCAATATTCTAA
  AGTTTTGCAACAATGAAGTGCTTTACTTAAGTGTTGATTGTGCCTGTTGTAAAGCTAATG
  AACCCTTTCCAATTATATGCTAATTAAATAATAAAAACTCCTATTTGCAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000847 unedited
  CGTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCCATTACGGCCGGGGGG
  TGGCCTTGAGAAGCTGAGCGGAGACCGGCTAGACTTTACTCAAAGAAACCAAGAGACTGT
  TACCATGGCAGGGAAGCCCAAGCTTCACTACTTCAATGGACGGGGCAGAATGGAGCCCAT
  CCGGTGGCTCTTGGCTGCAGCTGGAGTGGAGTTTGAAGAGAAATTTATAGGATCTGCAGA
  AGATTTGGGAAAGTTAAGAAATGATGGGAGTTTGATGTTCCAGCAAGTACCAATGGTTGA
  GATTGATGGGATTAAGTTGGTACAGACCAGAGCCATTCTCAACTACATTGCCAGCAAATA
  CAACCTCTACGGGAAAGACATAAAGGAGAGAGCCCTAATTGATATGTATACAGAAGGTAT
  GGCAGATTTGAATGAAATGATCCTTCTTCTGCCCTTATGTCGACCTGAGGAAAAAGATGC
  CAAGATTGCCTTGATCAAAGAGAAAACAAAAAGTCGCTATTTCCCTGCCTTCGAAAAAGT
  GTTACAGAGCCATGGACAAGACTACCTTGTTGGCAACAAGCTGAGCCGGGCTGACATTAG
  CCTGGTGGAACTTCTCTACTATGTGGAAGAGCTTGACTCCAGCCTTATCTCCAACTTCCC
  TCTGCTGAAGGCCCTNGANACCAGAAATCAGCACCTGCCCACGGTGAAAGAAGTTTCTAC
  AGCCTGGCAGCCCAAGGAAGCCTCCCGCAGATGCANAAGCTTTAGAAGAAGCAGAAAGAT
  TTTCCGTTTTTATAAAGCAGCCATGGGAGCTAAAGACATTGCAGACCAATATCTAAAGTT
  TTGCACAATGAAGTGCTTACTTAGTGGTGATGGGGCTGTTGTAAGCTATGGACCCTTTCC
  AATAATGCCAATAAATAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000847
• Insert Size: 918 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000847.3, NP_000838.3
• RefSeq Size: 937 bp
• RefSeq ORF: 669 bp
• Locus ID: 2940
• Cytogenetics: 6p12.2
• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
• Gene Summary: 'Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).