VMAT1 (SLC18A1) (NM_003053) Human Untagged Clone

CAT#: SC122643

SLC18A1 (untagged)-Human solute carrier family 18 (vesicular monoamine), member 1 (SLC18A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC18A1
• Synonyms: CGAT; VAT1; VMAT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003053 edited
  AGATCCACAAGTTTGTACAAAAAAGCAGGCTTGTAAAACGACGGCCAGTAACTATAACGG
  TCCTAAGGTAGCGAGGCCTGGGTGGCGAATTCGGCACGAGGCTGCTGTCTTGCTAACAGC
  TGCCAATACCTCACTGAGTGCCTCACACCAACATGGGCTCCAAGTGAGTTTCCTTCGTCT
  GGGCAGACTCCCTCCCCTCTTCCATAAAGGCTGCAGGAGACCTGTAGCTGTCACAGGACC
  TTCCCTAAGAGCCCGCAGGGGAAGACTGCCCCAGTCCGGCCATCACCATGCTCCGGACCA
  TTCTGGATGCTCCCCAGCGGTTGCTGAAGGAGGGGAGAGCGTCCCGGCAGCTGGTGCTGG
  TGGTGGTATTCGTCGCTTTGCTCCTGGACAACATGCTGTTTACTGTGGTGGTGCCAATTG
  TGCCCACCTTCCTATATGACATGGAGTTCAAAGAAGTCAACTCTTCTCTGCACCTCGGCC
  ATGCCGGAAGTTCCCCACATGCCCTCGCCTCTCCTGCCTTTTCCACCATCTTCTCCTTCT
  TCAACAACAACACCGTGGCTGTTGAAGAAAGCGTACCTAGTGGAATAGCATGGATGAATG
  ACACTGCCAGCACCATCCCACCTCCAGCCACTGAAGCCATCTCAGCTCATAAAAACAACT
  GCTTGCAAGGCACAGGTTTCTTGGAGGAAGAGATTACCCGGGTCGGGGTTCTGTTTGCTT
  CAAAGGCTGTGATGCAACTTCTGGTCAACCCATTCGTGGGCCCTCTCACCAACAGGATTG
  GATATCATATCCCCATGTTTGCTGGCTTTGTTATCATGTTTCTCTCCACAGTTATGTTTG
  CTTTTTCTGGGACCTATACTCTACTCTTTGTGGCCCGAACCCTTCAAGGCATTGGATCTT
  CATTTTCATCTGTTGCAGGTCTTGGAATGCTGGCCAGTGTCTACACTGATGACCATGAGA
  GAGGACGAGCCATGGGAACTGCTCTGGGGGGCCTGGCCTTGGGGTTGCTGGTGGGAGCTC
  CCTTTGGAAGTGTAATGTACGAGTTTGTTGGGAAGTCTGCACCCTTCCTCATCCTGGCCT
  TCCTGGCACTACTGGATGGAGCACTCCAGCTTTGCATCCTACAGCCTTCCAAAGTCTCTC
  CTGAGAGTGCCAAGGGGACTCCCCTCTTTATGCTTCTCAAAGACCCTTACATCCTGGTGG
  CTGCAGGGTCCATCTGCTTTGCCAACATGGGGGTGGCCATCCTGGAGCCCACACTGCCCA
  TCTGGATGATGCAGACCATGTGCTCCCCCAAGTGGCAGCTGGGTCTAGCTTTCTTGCCTG
  CCAGTGTGTCCTACCTCATTGGCACCAACCTCTTTGGTGTGTTGGCCAACAAGATGGGTC
  GGTGGCTGTGTTCCCTAATCGGGATGCTGGTAGTAGGTACCAGCTTGCTCTGTGTTCCTC
  TGGCTCACAATATTTTTGGTCTCATTGGCCCCAATGCAGGGCTTGGCCTTGCCATAGGCA
  TGGTGGATTCTTCTATGATGCCCATCATGGGGCACCTGGTGGATCTACGCCACACCTCGG
  TGTATGGGAGTGTCTACGCCATCGCTGATGTGGCTTTTTGCATGGGCTTTGCTATAGGTC
  CATCCACCGGTGGTGCCATTGTAAAGGCCATCGGTTTTCCCTGGCTCATGGTCATCACTG
  GGGTCATCAACATCGTCTATGCTCCACTCTGCTACTACCTGCGGAGCCCCCCGGCAAAGG
  AAGAGAAGCTTGCTATTCTGAGTCAGGACTGCCCCATGGAGACCCGGATGTATGCAACCC
  AGAAGCCCACGAAGGAATTTCCTCTGGGGGAGGACAGTGATGAGGAGCCTGACCATGAGG
  AGTAGCAGCAGAAGGTGCTCCTTGAATTCATGATGCCTCAGTGACCACCTCTTTCCCTGG
  GACCAGATCACCATGGCTGAGCCCACGGCTCAGTGGGCTTCACATACCTCTGCCTGGGAA
  TCTTCTTTCCTCCCCTCCCATGGACACTGTCCCTGATACTCTTCTCACCTGTGTAACTTG
  TAGCTCTTCCTCTATGCCTTGGTGCCGCAGTGGCCCATCTTTTATGGGAAGACAGAGTGA
  TGCACCTTCCCGCTGCTGTGAGGTTGATTAAACTTGAGCTGTGACGGGTTCTGCAAGGGG
  TGACTCATTGCATAGAGGTGGTAGTGAGTAATGTGCCCCTGAAACCAGTGGGGTGACTGA
  CAAGCCTCTTTAATCTGTTGCCTGATTTTCTCTGGCATAGTCCCAACAGATCGGAAGAGT
  GTTACCCTCTTTTCCTCAACGTGTTCTTTCCCGGGTTTTCCCAGCCGAGTTGAGAAAATG
  TTCTCAGCATTGTCTTGCTGCCAAATGCCAGCTTGAAGAGTTTTGTTTTGTTTTTTTTCC
  ATTTATTTTTTTTTTTTAATAAAGTGAGTGATTTTTCTGTGGCTAAATCTAGAGCTGCTA
  AAAGGGCTTTACCCTCAGTGAAAAGTGTCTTCTATTTTCATTATCTTTCAGAAACAGGAG
  CCCATTTCTCTTCTGCTGGAGTTATTGACATTCTCCTGACCTCCCCTGTGTGTTCCTACC
  TTTTCTGAACCTCTTAGACTCTTAGAAATAAAAGTAGAAGAAAGACAGAAAAAATAACTG
  ATTAGACCCAAGATTTCATGGGAAGAAGTTAAAAGAAACTGCCTTGAAATCCCTCCTGAT
  TGTAGATTTCCTAATAGGAGGGGTGTAATGTGACATTGTTCATACTTGCTAATAAATACA
  TTATTGCCTAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_003053
• Insert Size: 2797 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_003053.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003053.1, NP_003044.1
• RefSeq Size: 2749 bp
• RefSeq ORF: 1578 bp
• Locus ID: 6570
• Cytogenetics: 8p21.3
• Protein Families: Transmembrane
• Protein Pathways: Parkinson's disease
• Gene Summary: 'The vesicular monoamine transporter acts to accumulate cytosolic monoamines into vesicles, using the proton gradient maintained across the vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine (Peter et al., 1993 [PubMed 7905859]). See also SLC18A2 (MIM 193001).[supplied by OMIM, Mar 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein (isoform a).